Polypeptides comprising an ice-binding activity

ABSTRACT

The present invention relates to novel polypeptides comprising an ice-binding capability resulting in an ice crystal formation and/or growth reducing or inhibiting activity. The present invention also relates to an edible product and to a solid support comprising the novel polypeptide. Furthermore, the present invention also relates to a method for producing the novel polypeptide and to different uses of the novel polypeptide.

This application is a non-provisional of U.S. provisional application Ser. No. 61/003,979 filed on Nov. 21, 2007, which is hereby incorporated by reference in its entirety. All patent and non-patent references cited in the afore-mentioned provisional application, or in the present application, are hereby incorporated by reference in their entirety.

FIELD OF INVENTION

The present invention relates to novel polypeptides comprising an ice-binding capability resulting in an ice crystal formation and/or growth reducing or inhibiting activity. Methods for producing and using such polypeptides are also disclosed.

BACKGROUND OF INVENTION

The body temperature of marine teleosts living in polar waters is in temperature equilibrium with the surrounding sea, and is thus at a temperature of approximately −1.8° C. during winter or year round in for example Antarctic waters. The blood of marine teleosts is hypoosmotic to the seawater and its melting point is predicted to be approximately −0.7° C. The polar teleosts are thus supercooled and lethal freezing would be expected in the absence of an adaptation to such harsh climatic conditions.

Similar observations are applicable for a variety of cold-adapted terrestrial organisms, including arthropods, plants, fungi and bacteria although many of these organisms are subjected to much lower temperatures than marine fish. Psychrophilic (cold-loving) organisms have successfully adapted to all the permanently cold regions on earth: They can be found in the deep sea, on freezing mountain tops and in the Polar regions even at temperatures as low as −60° C. Despite the lethal conditions, these organisms have overcome key barriers inherent to permanently cold environments. These barriers include: reduced enzyme activity, decreased membrane fluidity, altered transport of nutrients and waste products, decreased rates of transcription, translation and cell division, polypeptide cold-denaturation, inappropriate polypeptide folding and intracellular ice formation.

Research on the mechanisms that allow certain organisms to exist at subzero temperatures has revealed that they rely on at least two strategies: Lowering of the freezing point of water (colligatively by synthesis of low molecular weight substances and non-colligatively via synthesis of unique polypeptides) by either inhibiting ice growth or by giving rise to controlled ice crystal formation. Anti-freeze polypeptides (AFPs) and low molecular weight substances, such as polyalcohols, free amino acids and sugars are believed to be responsible for the former process, while Ice Nucleating polypeptides (INPs) are responsible for the latter.

Anti-freeze polypeptides (AFP—in some publications also known as thermal hysteresis polypeptides, THP, or ice structuring polypeptides, ISP) lower the freezing point of a solution substantially while the predicted melting point is only moderately depressed. This means that whereas the freezing point is lowered dramatically, the melting point of the solution is predicted by the colligative melting point depression. This is true for solutions where ice is present—the question as to whether anti-freeze polypeptides can lower the supercooling point of an ice-free solution is largely unsolved.

The displacement of the freezing temperature is limited and rapid ice growth will take place at a sufficiently low temperature. The separation of the melting and freezing temperature is usually referred to as thermal hysteresis (TH) (Knight et al. 1991, Raymond and DeVries 1977, Wilson 1993), and the temperature of ice growth is referred to as the hysteresis freezing point. The difference between the melting point and the hysteresis freezing point is called the hystersis or the anti-freeze activity. A second functionality of the AFPs is in the frozen state, where they show ice recrystallization inhibition (RI). The AFPs inhibit the formation of large crystals at the expense of small crystals at temperatures above the temperature of recrystrallisation. (Knight et 1.1984, 1995, Knight and Duman 1986, Ramlov et al. 1996).

Mechanism of Inhibition of Ice Formation

The mechanism by which anti-freeze polypeptides inhibit ice growth is still under investigation. AFPs seem all to be amphiphilic. This means that they have one part which is more hydrophobic than the rest of the molecule. Hitherto the explanation for their activity is that their hydrophilic side binds to the ice crystal. However, this view has during the last decade been challenged as when looking at ice/water one can with good reason ask which is per definition most hydrophilic-ice or water. Various evidence for the binding of the AFPs to the ice via their hydrophobic side/domains is emerging, but as the exact mechanism for the binding is not known all evidence so far has been circumstantial.

However, consensus at this point in time is that the anti-freeze polypeptides recognize and bind to various ice surface planes, depending on the type (and isoform) of anti-freeze polypeptide. Ice growth stops where the anti-freeze polypeptides are bound, but continues to a certain extent between the anti-freeze molecules (Raymond and DeVries 1977, DeLuca et al. 1998, Marshall et al. 2004). When the curvature of the ice growing between the anti-freeze polypeptide molecules becomes sufficiently large (or curved), ice growth stops due to the increased surface tension of the curved surfaces (known as the Kelvin effect (Atkins and De Paula 2002, Wilson 1994, 2005, Kristiansen 2005)). It is now not energetically favourable for the water molecules to bind to the curved ice surfaces.

The hysteresis freezing point is thus the temperature where it again becomes energetically favourable for the water molecules to bind to the ice and ice growth continues explosively (Knight et al. 1991, Raymond and DeVries 1977, Wilson 1993).

In most fish anti-freeze solutions, spicular ice growth is seen at the hysteresis freezing point. It is assumed that this is due to the fact that the fish anti-freeze polypeptides bind to the prismplanes on the ice crystals but not to the basal planes. Growth at the hysteresis freezing point is in this case due to binding (addition) of water molecules to the basal planes where growth at the prism planes is still inhibited, thus the ice crystals grow like long spears (spicules).

In solutions containing anti-freeze polypeptides from insects the growth pattern at the hysteresis freezing point is more random and it is suggested that the reason for this is that insect anti-freeze polypeptides also bind to the basal planes (also giving rise to the much higher anti-freeze activity observed in solutions from these animals) and growth thus occurs at spots at any place on the ice crystals once the temperature is low enough (the hysteresis freezing point).

When ice growth is occurring at the hysteresis freezing point in the presence of insect anti-freeze polypeptides the ice growth pattern is cauliflower like in stead of spicular. The thermal hysteresis is thus dependent on at least 2 parameters: 1) the type of anti-freeze polypeptide (dependent on organism, isoform) and 2) the concentration, albeit that the concentration dependency shows saturation (DeVries 1983, Kao et al. 1985, Schrag et al. 1982).

Apparently, there is also a positive correlation between the size of the anti-freeze polypeptide molecules and the amount of hysteresis observed (Kao et al. 1985, Schrag et al. 1982, Wu et al. 2001). Apart from the above mentioned parameters determining the anti-freeze effect it is also substantiated that there is a reciprocal relationship between anti-freeze activity and ice crystal fraction (when the ice crystal is within the hysteresis gap). This effect is most noticeable in the case of insect anti-freeze polypeptides (Zachariassen et al. 2002).

Insect-Derived Anti-Freeze Polypeptides

Several AFPs have been found in insects. This is presumably due to the exposure of insects to much lower temperatures than those which fish may encounter (the freezing point of sea water is approximately −1.8° C.).

To date, the structure of insect AFPs has been elucidated for 3 species: Tenebrio molitor, Choristoneura fumiferana and Dendroides canadensis. Two of the three characterized AFPs from insects (from T. molitor and D. canadensis) have many structures in common, presumably because they both come from beetles, whereas C. fumiferana is a moth.

An example of an insect AFP that is very well characterized is the AFP from the beetle Tenebrio molitor. This polypeptide is found in a least 9 isoforms which are all very much alike, but with lengths from 84, 96 to 120 amino acids (Liou et al. 1999). In all the 9 known isoforms a repetitive sequence of 12 amino acids is found: T/A-C-T-X-S-X-X-C-X-X-A-X. This sequence is repeated 6 to 9 times in these AFPs. TmAFP is a right handed beta-helix structure of repetitive amino acid sequences. A highly regular array of threonine residues on the flat beta sheet is thought to interact with water/ice, as the distances between the threonine residues is predicted to fit exactly with the oxygen atom positions in the water molecules of the ice lattice structure. This AFP has an extremely regular structure and a predicted high structural stability provided by a high number for cysteine-cysteine sulphur bridges; every sixth amino acid is a cysteine.

From D. canadensis 13 isoforms with varying length and weight (7.3-12.3 kDa) have been isolated (Andorfer and Duman 2000, Duman et al. 2002). In these AFPs the repeating sequence is the same as is found in T. molitor albeit a 13eth amino acid is sometimes present in some of the repeating sequences ((Duman 1998, Li et al 1998a, 1998b). The cysteines are placed exactly in the same positions as in the AFPs from T. molitor (Li et al. 1998a, 1998b).

The amino acid sequences of the AFPs from C. fumiferana are not homologous to the two other known AFP amino acid sequences from insects. However, a sequence T-X-T is found at every 15th amino acid but only the last threonine is conserved; the first Threonine are in many cases substituted with valine (V), arginine (R) or isoleucine (I). The AFPs from C. fumiferana also contain fewer cysteines than the other two insect AFPs (Doucet et al. 2000). Howvever, all cysteines participate in disulfide bindings (Gauthier et al. 1998). Apparently the AFPs from C. fumiferana contains a hydrophobic core and the polypeptides are stabilized by a network of hydrogen bonds and the disulfide bridges, which all together stabilises the structure (Graether et al. 2000, 2003, Leinala et al 2002).

SUMMARY OF THE INVENTION

The present invention is directed in one aspect to anti-freeze polypeptides and fragments thereof, including anti-freeze polypeptides produced by certain Cerambycid bark beetles and comprising a plurality of ice-binding sites. Methods for making and using such polypeptides, as disclosed herein below in more detail, are also within the scope of the present invention.

The Cerambycid bark beetles Rhagium inquisitor and Rhagium mordax, both in their adult and larval stages, can be subjected to temperatures as low as −30° C. and still overwinter under bark on tree stumps in northern Scandinavia. Both of these species belong to the group of cold tolerant animals called freeze avoiders, which means that they survive the extremely low temperatures without ice formation in their tissues. They can be regarded to adopt a supercooled (or undercooled) conditions for long time periods.

The supercooled state is potentially lethal to the Cerambycid bark beetles as ice formation in a metastable, supercooled liquid can in principle occur due to random nucleation or as a consequence of inoculation of ice from the surroundings. The Cerambycid bark beetles cannot survive such an ice formation.

Accordingly, both R. inquisitor and R. mordax have adapted to the climatic conditions and they are able to survive at low temperatures for extended time periods. This makes the Cerambycid bark beetles interesting as a source for anti-freeze polypeptides (AFPs).

In R. inquisitor, adaptation to low temperatures has involved accumulation of cryoprotective glycerol in the haemolymph regions, elimination of potential ice nucleating agents from body fluids (giving a much higher probability of supercooling) and synthesis of a number of anti-freeze polypeptides prior to the onset of winter. So far, the anti-freeze activity (7° C.) of the anti-freeze polypeptides found in R. inquisitor has been considered the highest known.

Larvae of R. mordax are capable of surviving almost as cold temperatures as R. inquisitor—in spite of having a very limited accumulation of cryoprotective compounds. According to one presently preferred hypothesis, the ability of Rhagium mordax to withstand low temperatures can almost exclusively be ascribed to the synthesis and accumulation of one or more anti-freeze polypeptides (AFP's) prior to the onset of winter.

Anti-freeze polypeptides according to the present invention are surprisingly found to have a significantly lower number of cysteine residues than other insect AFPs presently known. This feature, together with the fact that the polypeptides according to the present invention have fewer repeated sequences than many state-of-the-art insect anti-freeze polypeptides, make them better candidates for expression in heterologous host organisms.

In presently preferred embodiments, the anti-freeze polypeptides according to the present invention, and functional fragments thereof exhibiting anti-freeze activity, preferably have less than 4 cysteine residues, such as less than 3 cysteine residues, for example 2 cysteine residues.

The anti-freeze polypeptides according to the present invention have a variety of utilities and industrial applications as will be clear from the below disclosure. The polypeptides, or genes encoding the polypeptides, can be used in various ways to suppress ice crystal growth. The polypeptides may be introduced directly, or they may be introduced as a gene which is expressed in a host cell under the control of a suitable expression signal to produce the polypeptide(s).

Suitable concentrations of anti-freeze polypeptides will vary depending on the use, but will typically be in the range of from about one part per billion to about one part per thousand (i.e., from about 1 μg/l to about 1 mg/l).

In some aspects of the present invention, the polypeptides are introduced into edible products, or brought into contact with edible products, so as to reduce or inhibit ice crystal growth and/or formation e.g. during production and/or storage of the edible products in their frozen condition.

It has surprisingly been found that the polypeptides according to the present invention provides ice crystallisation that are markedly different than crystals obtained in the presence of other known anti-freeze polypeptides. In particular it has been found that in the presence of the polypeptides according to the present invention crystals with a small spheric structure are obtained while known anti-freeze polypeptides such as e.g. anti-freeze protein type III HPLC 12 mentioned in U.S. Pat. No. 6,914,043 or the ice crystal growth inhibiting agent as mentioned in U.S. Pat. No. 6,312,733 provides ice crystals with a spicular structure.

Hence, one major advantage of the present invention is that when the polypeptides according to the present invention are incorporated into e.g. ice cream an improved mouth feel is obtained due to the fact that the crystals formed in the ice cream during production and storage will have an essentially small spheric structure compared to the rough spicular crystals obtained when using known anti-freeze polypeptides of type III of herein above.

The texture, taste, and useful storage life of frozen edible products, including vegetables, will be greatly improved as a result of the action of the polypeptides according to the present invention. For example, the texture, taste, and useful storage life of frozen vegetables, such as e.g. celery, potatoes, asparagus, peas, carrots, beans, broccoli, sweet corn, spinach, and the like, will be improved. Similarly, the texture, taste and useful storage life of various frozen fruits will be enhanced, including strawberries, blueberries, raspberries, citrus fruits, bananas, grapes, kiwis, peaches, pineapples, plums, cherries, tomatoes and mangoes.

The introduction into vegetables, and other edible products, can be accomplished e.g. by genetic introduction of appropriate polynucleotides into the target organism. Expression of a polynucleotide, either constitutively or inducibly, before food processing has begun, or after harvesting and processing has begun, results in sufficiently high levels of polypeptides according to the present invention to effectively protect the edible product, including a food product, such as up to about 0.1% of total plant polypeptide by mass. Expression can also be on a tissue specific basis. For example, linkage to ripening genes in fruits may result in expression even after harvesting from the producing plant.

The polypeptides, in one important aspect of the invention, are added to foods which are expected to be or remain frozen until, or even during, consumption—and in particular edible products which are consumed in a frozen or cold state.

Many frozen food products are intended to be consumed in the frozen or cold state, for example, ice cream, frozen yogurt, ice milk, sherbet, popsicles, frozen whipped cream, frozen cream pies, frozen puddings and the like. In particular, texture and flavour are adversely affected by the formation of large ice crystals throughout a freeze-thaw cycle that occurs in most home frost-free freezers, or upon sustained storage in the frozen state. This ice crystal growth process may be reduced or even prevented entirely, or at least minimized, by using the anti-freeze polypeptides according to the present invention. The anti-freeze polypeptides according to the present invention may be either incorporated throughout the edible product, and/or they may, i.e. such as alternatively, be applied to the surface of the edible product, where condensation and ice crystal formation is expected to occur most readily.

Another important aspect of the present invention relates to yeasts, including bakers yeast, comprising polynucleotides encoding the polypeptides according to the present invention. Methods related to this aspect include methods for transform dough yeast with polynucleotides encoding these polypeptides. Upon incorporation and expression of the polynucleotides into the yeasts, and use of these yeasts e.g. in frozen dough, the dough will naturally leaven upon thawing because the yeast viability will remain high upon thawing. Because less damage accumulates from storage in the presence of these anti-freeze polypeptides and because thawed samples will have preserved a high viability of the yeast cells, either longer storage times in a frozen state will be possible, or smaller samples of dough will need to be stored.

An alternative way of incorporating anti-freeze polypeptides into frozen, fermented edible products is to have the organism responsible for the fermentation process produce the anti-freeze polypeptides while fermenting the food. Hence, the present invention also embraces methods for preparing a frozen fermented food product. This method comprises the steps of (a) contacting a food product with a microorganism that is capable of secreting a polypeptide according to the present invention, wherein the microorganism is capable of fermenting the food product to produce the fermented food product, (b) incubating the food product with the microorganism under conditions in which fermentation takes place so that a fermented food product is produced having anti-freeze polypeptides according to the present invention present in an amount effective for inhibiting ice crystal growth and/or formation in the product or on the surface of the product; and (c) freezing the fermented food product at a temperature of preferably below −5° C., so as to produce a frozen, fermented food product.

Yet another aspect of the present invention is directed to the introduction of anti-freeze polypeptides according to the present invention present into biological cells, or extracts thereof destined for frozen storage. For example, bacterial cells, yeast cells, plant cells and animal cells comprising the anti-freeze polypeptides according to the present invention present have an increased cell or tissue viability with minimal or no loss of inherent characteristics due to the freeze-thaw process. Sub-cellular samples or cellular extracts may have similar sensitivities to freezing, especially on prolonged storage. Typical examples will be in vitro polypeptide translation systems, enzyme preparations, and particularly samples which contain sensitive membrane components, such as chloroplast or mitochondrial membrane preparations.

In particular, samples containing organelles may display increased resistance to freezing damage upon addition of the anti-freeze polypeptides according to the present invention present. Soft animal tissues will exhibit less damage upon freezing in the presence of the subject polypeptides, and addition of the polypeptides according to the present invention present will be useful in situations, when cellular integrity upon freezing and subsequent thawing is important or desired, such as for tissue culture deposits. Thus, samples destined for frozen storage, such as for cell or tissue depositories, might routinely have the polypeptides according to the present invention present added to them. Among the biological cell types often stored are genetic variants of bacteria, fungi (including yeast), and, particularly, higher eucaryote cells (such as hybridoma strains and tissue culture cell lines).

The present invention in other aspects are directed to applications which are not specific to the food area. One non-food application of the polypeptides according to the present invention present is the protection of crops and plants from climatic freezing conditions. The anti-freeze polypeptides according to the present invention present may be either internally incorporated into the cytoplasm by expression of an introduced gene, or the polypeptides may be externally applied to the plants—e.g. by spraying or otherwise. External application may thus be achieved either by direct application of the polypeptides to the plant, or by the external deposit onto the plant of an organism which secretes the polypeptide. These same alternatives for introduction apply to other uses as well.

Another embodiment is the introduction of an anti-freeze polypeptide into a liquid surrounding an organ, tissue or other biological sample. One particular use would be during transportation to a hospital for a transplantation operation or for storage purposes. The anti-freeze polypeptide according to the present invention present should allow short- or long-term storage at a subfreezing temperature, thereby minimizing inherent metabolism or degradation, but with substantially diminished cellular damage from ice crystal growth. Other medically important temperature sensitive biological samples are blood and blood products, therapeutic agents, polypeptide drugs, bioassay reagents and vaccines.

The present invention also provides a cosmetic or dermatological preparation which comprises the polypeptides according to the present invention. The use of the polypeptides according to the present invention in cosmetic or topical dermatological preparations renders possible an effective treatment, but also a prophylaxis of structural and cellular damage in the skin due to cold, which damage with distinct climate- and weather-induced drops in temperature cause changes in the cell physiology in the cell and in the extracellular space through loss of the temperature optima of cellular enzymes, skin damage, skin redness and tight feeling of the skin and increased sensory sensitivities, induced, e.g., by cold, wind and/or UV light, temperature-sensitive skin, negative changes in the skin, the lips and the mucous membranes in the nose and mouth area and the integumentary appendage caused by environmental stress (caused by temperature changes and UV light, smoking, smog, reactive oxygen species, free radicals).

Also included in the present invention are compositions and uses based on the mixture of anti-freeze polypeptides according to the present invention with state-of-the-art stabilizers, emulsifiers and surfactants well known to those skilled in the art and other additives. These compounds may be present to inhibit decay, inhibit oxidation, prevent discoloration, inhibit microbial growth, stabilize emulsions and so forth.

As will be clear from the above, the present invention is directed in one aspect to polypeptides capable of inhibiting and/or reducing ice crystal formation associated with the freezing or supercooling of an object or substance, including an edible product. Supercooling conditions are conditions allowing the cooling of a substance below the temperature at which a change of state would ordinarily take place (i.e. from a water phase to ice) without such a change of state occurring. Accordingly, the cooling of a liquid below its freezing point without freezing taking place constitutes supercooling and results in a metastable state.

The polypeptides according to the present invention will interchangeably be denoted anti-freeze polypeptides and, for short, polypeptides, throughout the present description.

In one aspect there is provided a polypeptide selected from the group consisting of SEQ ID NO:1 to SEQ ID NO:8. The annotation SEQ ID NO:1 to SEQ ID NO:8, and other similar annotations indicating a starting number and an end number of a range of sequence identity numbers, shall, when used herein, denote each and every one of said sequence identity numbers, such as, in the above cited example, the sequence identity numbers SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8, unless otherwise noted.

Polypeptides comprising or consisting of any of SEQ ID NO:1 to SEQ ID NO:8, fragments thereof having anti-freeze activity, and variants thereof being at least about 75% identical to any of SEQ ID NO:1 to SEQ ID NO:8, or a fragment thereof, also fall within the scope of the present invention. In particular, a fragment of the polypeptide comprising or consisting of any of SEQ ID NO:1 to SEQ ID NO:8 having at least 20 amino acids and an ice binding and anti-freeze activity are also disclosed. The fragment preferably has less than 200 amino acids, such as preferably less than 150 amino acids, for example preferably less than 100 amino acids, such as 80 amino acids, for example 60 amino acids.

The invention is also directed to polypeptides comprising one or more “ice binding sites” (IBSs) according to the present invention, such as polypeptides comprising one or more of any of SEQ ID NO:9 to SEQ ID NO: 72—a total of 64 specific “ice binding sites”, IBSs, as indicated in FIG. 3.

In particular, the present invention is directed towards the following polypeptides:

A polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 9 to SEQ ID NO:16.

A polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 17 to SEQ ID NO:24.

A polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 25 to SEQ ID NO:32.

A polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 33 to SEQ ID NO:40.

A polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 41 to SEQ ID NO:48.

A polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 49 to SEQ ID NO:56.

A polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 57 to SEQ ID NO:64.

A polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 65 to SEQ ID NO:72.

There is also provided polypeptides comprising one or more of the sequences SEQ ID NO: 73 to 80, such as 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences of SEQ ID NO: 73 to 80—in the form of conserved “ice binding domains”, IBDs.

SEQ ID NO: 81 to 89 relate to further conserved domains which may also be present, singly, or in any combination, in the polypeptides according to the present invention.

The present invention is also directed to SEQ ID NO: 90, in the form of a general ice binding domain, general IBD, which can be present a number of times, such as 2 times, 3 times, 4 times, 5 times, 6 times, 7 times, 8 times, more than 8 times and preferably less than 30 times in the polypeptides according to the present invention. Identical sequences can be present a plurality of times, or variants of SEQ ID NO:90 can be present a plurality of times, as disclosed herein below in the section citing items of the present invention.

Polypeptides comprising pair-wise combinations of ice binding domains are listed in Table 1 herein below. A total of 64 different combinations are listed—in the form of each and every pair-wise and independent combination of any of the 8 ice binding domains (IBDs) disclosed herein as SEQ ID NO: 73 to SEQ ID NO:80.

The present invention is also directed to a polypeptide comprising 4 or more sequences, such as 5 or more sequences, for example 6 or more sequences, such as 7 or all of the sequences selected from the group consisting of SEQ ID NO: 73 to SEQ ID NO:80

In a further aspect, there is provided a polypeptide comprising 4 or more copies, such as 5 or more copies, for example 6 or more copies, such as 7 or more copies, for example 8 or more copies, and preferably less than 20 copies, of the sequence SEQ ID NO: 90.

As stated herein above, the present invention in one aspect is directed to a polypeptide comprising a combination of different ice binding domains (IBDs), said combination comprising as least 1 combination, such as at least 2 combinations, of any of the combinations of 2 IBDs selected from the group of 64 pair-wise ice binding domain sequence combinations presented in Table 1, herein immediately below, said polypeptide preferably having less than 20 general ice binding domains, such as less than 15 general ice binding domains, for example less than 12 general ice binding domains, such as less than 10 general ice binding domains defined by SEQ ID NO:90.

TABLE 1 IBD I IBD II IBD III IBD IV IBD V IBD VI IBD VII IBD VIII IBD I A1 A2 A3 A4 A5 A6 A7 A8 IBD II B1 B2 B3 B4 B5 B6 B7 B8 IBD III C1 C2 C3 C4 C5 C6 C7 C8 IBD IV D1 D2 D3 D4 D5 D6 D7 D8 IBD V E1 E2 E3 E4 E5 E6 E7 E8 IBD VI F1 F2 F3 F4 F5 F6 F7 F8 IBD VII G1 G2 G3 G4 G5 G6 G7 G8 IBD VIII H1 H2 H3 H4 H5 H6 H7 H8 IBD: Ice Binding Domain Each of the sequences SEQ ID NO: 1 to SEQ ID NO: 8 contains a total of 8 IBDs, denoted I, II, III, IV, V, VI, VII and VIII, herein above in Table 1. In Table 1 IBD I represents SEQ ID NO 73, IBD II represents SEQ ID NO 74, IBD III represents SEQ ID NO 75, IBD IV represents SEQ ID NO 76, IBD V represents SEQ ID NO 77, IBD VI represents SEQ ID NO 78, IBD VII represents SEQ ID NO 79 and IBD VIII represents SEQ ID NO 80.

Each of the separate letter/number combinations presented in the matrix denoted Table 1 herein above is cross-combined with each other to produce the combinations listed herein below in Table 2. To exemplify, the combination A1A1 originates from the combination of two IBD I domains with another two IBD I domains whereas the combination B3A6 originates from the combination of one IBD II and one IBD III with one IBD I and one IBD VI. The resulting polypeptide thus comprises the following polypeptide ice binding domains: IBD I, IBD II, IBD III and IBD VI.

The present invention thus further provides a polypeptide comprising a combination of different ice binding domains (IBDs), said combination comprising as least 1 combination, such as at least 2 combinations, for example 3 combinations of any of the combination of 4 IBDs selected from the group of 4096 combinations of any 4 ice-binding domain sequences presented in Table 2, herein immediately below, said polypeptide preferably having less than 20 general ice binding domains, such as less than 15 general ice binding domains, for example less than 12 general ice binding domains, such as less than 10 general ice binding domains defined by SEQ ID NO:90.

To further exemplify Table 2, the combinations originates from a 64×64 matrix and A1A1 is the combination of two IBD I domains with another two IBD I domains whereas the combination B3A6 is originated from the combination of one IBD II and one IBD III domains with one IBD I and one IBD VI domain. The later thereby consisting of the following polypeptide domains: IBD I, IBD II, IBD III and IBD VI.

TABLE 2 A1A1 A1A2 A1A3 A1A4 A1A5 A1A6 A1A7 A1A8 A2A1 A2A2 A2A3 A2A4 A2A5 A2A6 A2A7 A2A8 A3A1 A3A2 A3A3 A3A4 A3A5 A3A6 A3A7 A3A8 A4A1 A4A2 A4A3 A4A4 A4A5 A4A6 A4A7 A4A8 A5A1 A5A2 A5A3 A5A4 A5A5 A5A6 A5A7 A5A8 A6A1 A6A2 A6A3 A6A4 A6A5 A6A6 A6A7 A6A8 A7A1 A7A2 A7A3 A7A4 A7A5 A7A6 A7A7 A7A8 A8A1 A8A2 A8A3 A8A4 A8A5 A8A6 A8A7 A8A8 B1A1 B1A2 B1A3 B1A4 B1A5 B1A6 B1A7 B1A8 B2A1 B2A2 B2A3 B2A4 B2A5 B2A6 B2A7 B2A8 B3A1 B3A2 B3A3 B3A4 B3A5 B3A6 B3A7 B3A8 B4A1 B4A2 B4A3 B4A4 B4A5 B4A6 B4A7 B4A8 B5A1 B5A2 B5A3 B5A4 B5A5 B5A6 B5A7 B5A8 B6A1 B6A2 B6A3 B6A4 B6A5 B6A6 B6A7 B6A8 B7A1 B7A2 B7A3 B7A4 B7A5 B7A6 B7A7 B7A8 B8A1 B8A2 B8A3 B8A4 B8A5 B8A6 B8A7 B8A8 C1A1 C1A2 C1A3 C1A4 C1A5 C1A6 C1A7 C1A8 C2A1 C2A2 C2A3 C2A4 C2A5 C2A6 C2A7 C2A8 C3A1 C3A2 C3A3 C3A4 C3A5 C3A6 C3A7 C3A8 C4A1 C4A2 C4A3 C4A4 C4A5 C4A6 C4A7 C4A8 C5A1 C5A2 C5A3 C5A4 C5A5 C5A6 C5A7 C5A8 C6A1 C6A2 C6A3 C6A4 C6A5 C6A6 C6A7 C6A8 C7A1 C7A2 C7A3 C7A4 C7A5 C7A6 C7A7 C7A8 C8A1 C8A2 C8A3 C8A4 C8A5 C8A6 C8A7 C8A8 D1A1 D1A2 D1A3 D1A4 D1A5 D1A6 D1A7 D1A8 D2A1 D2A2 D2A3 D2A4 D2A5 D2A6 D2A7 D2A8 D3A1 D3A2 D3A3 D3A4 D3A5 D3A6 D3A7 D3A8 D4A1 D4A2 D4A3 D4A4 D4A5 D4A6 D4A7 D4A8 D5A1 D5A2 D5A3 D5A4 D5A5 D5A6 D5A7 D5A8 D6A1 D6A2 D6A3 D6A4 D6A5 D6A6 D6A7 D6A8 D7A1 D7A2 D7A3 D7A4 D7A5 D7A6 D7A7 D7A8 D8A1 D8A2 D8A3 D8A4 D8A5 D8A6 D8A7 D8A8 E1A1 E1A2 E1A3 E1A4 E1A5 E1A6 E1A7 E1A8 E2A1 E2A2 E2A3 E2A4 E2A5 E2A6 E2A7 E2A8 E3A1 E3A2 E3A3 E3A4 E3A5 E3A6 E3A7 E3A8 E4A1 E4A2 E4A3 E4A4 E4A5 E4A6 E4A7 E4A8 E5A1 E5A2 E5A3 E5A4 E5A5 E5A6 E5A7 E5A8 E6A1 E6A2 E6A3 E6A4 E6A5 E6A6 E6A7 E6A8 E7A1 E7A2 E7A3 E7A4 E7A5 E7A6 E7A7 E7A8 E8A1 E8A2 E8A3 E8A4 E8A5 E8A6 E8A7 E8A8 F1A1 F1A2 F1A3 F1A4 F1A5 F1A6 F1A7 F1A8 F2A1 F2A2 F2A3 F2A4 F2A5 F2A6 F2A7 F2A8 F3A1 F3A2 F3A3 F3A4 F3A5 F3A6 F3A7 F3A8 F4A1 F4A2 F4A3 F4A4 F4A5 F4A6 F4A7 F4A8 F5A1 F5A2 F5A3 F5A4 F5A5 F5A6 F5A7 F5A8 F6A1 F6A2 F6A3 F6A4 F6A5 F6A6 F6A7 F6A8 F7A1 F7A2 F7A3 F7A4 F7A5 F7A6 F7A7 F7A8 F8A1 F8A2 F8A3 F8A4 F8A5 F8A6 F8A7 F8A8 G1A1 G1A2 G1A3 G1A4 G1A5 G1A6 G1A7 G1A8 G2A1 G2A2 G2A3 G2A4 G2A5 G2A6 G2A7 G2A8 G3A1 G3A2 G3A3 G3A4 G3A5 G3A6 G3A7 G3A8 G4A1 G4A2 G4A3 G4A4 G4A5 G4A6 G4A7 G4A8 G5A1 G5A2 G5A3 G5A4 G5A5 G5A6 G5A7 G5A8 G6A1 G6A2 G6A3 G6A4 G6A5 G6A6 G6A7 G6A8 G7A1 G7A2 G7A3 G7A4 G7A5 G7A6 G7A7 G7A8 G8A1 G8A2 G8A3 G8A4 G8A5 G8A6 G8A7 G8A8 H1A1 H1A2 H1A3 H1A4 H1A5 H1A6 H1A7 H1A8 H2A1 H2A2 H2A3 H2A4 H2A5 H2A6 H2A7 H2A8 H3A1 H3A2 H3A3 H3A4 H3A5 H3A6 H3A7 H3A8 H4A1 H4A2 H4A3 H4A4 H4A5 H4A6 H4A7 H4A8 H5A1 H5A2 H5A3 H5A4 H5A5 H5A6 H5A7 H5A8 H6A1 H6A2 H6A3 H6A4 H6A5 H6A6 H6A7 H6A8 H7A1 H7A2 H7A3 H7A4 H7A5 H7A6 H7A7 H7A8 H8A1 H8A2 H8A3 H8A4 H8A5 H8A6 H8A7 H8A8 A1B1 A1B2 A1B3 A1B4 A1B5 A1B6 A1B7 A1B8 A2B1 A2B2 A2B3 A2B4 A2B5 A2B6 A2B7 A2B8 A3B1 A3B2 A3B3 A3B4 A3B5 A3B6 A3B7 A3B8 A4B1 A4B2 A4B3 A4B4 A4B5 A4B6 A4B7 A4B8 A5B1 A5B2 A5B3 A5B4 A5B5 A5B6 A5B7 A5B8 A6B1 A6B2 A6B3 A6B4 A6B5 A6B6 A6B7 A6B8 A7B1 A7B2 A7B3 A7B4 A7B5 A7B6 A7B7 A7B8 A8B1 A8B2 A8B3 A8B4 A8B5 A8B6 A8B7 A8B8 B1B1 B1B2 B1B3 B1B4 B1B5 B1B6 B1B7 B1B8 B2B1 B2B2 B2B3 B2B4 B2B5 B2B6 B2B7 B2B8 B3B1 B3B2 B3B3 B3B4 B3B5 B3B6 B3B7 B3B8 B4B1 B4B2 B4B3 B4B4 B4B5 B4B6 B4B7 B4B8 B5B1 B5B2 B5B3 B5B4 B5B5 B5B6 B5B7 B5B8 B6B1 B6B2 B6B3 B6B4 B6B5 B6B6 B6B7 B6B8 B7B1 B7B2 B7B3 B7B4 B7B5 B7B6 B7B7 B7B8 B8B1 B8B2 B8B3 B8B4 B8B5 B8B6 B8B7 B8B8 C1B1 C1B2 C1B3 C1B4 C1B5 C1B6 C1B7 C1B8 C2B1 C2B2 C2B3 C2B4 C2B5 C2B6 C2B7 C2B8 C3B1 C3B2 C3B3 C3B4 C3B5 C3B6 C3B7 C3B8 C4B1 C4B2 C4B3 C4B4 C4B5 C4B6 C4B7 C4B8 C5B1 C5B2 C5B3 C5B4 C5B5 C5B6 C5B7 C5B8 C6B1 C6B2 C6B3 C6B4 C6B5 C6B6 C6B7 C6B8 C7B1 C7B2 C7B3 C7B4 C7B5 C7B6 C7B7 C7B8 C8B1 C8B2 C8B3 C8B4 C8B5 C8B6 C8B7 C8B8 D1B1 D1B2 D1B3 D1B4 D1B5 D1B6 D1B7 D1B8 D2B1 D2B2 D2B3 D2B4 D2B5 D2B6 D2B7 D2B8 D3B1 D3B2 D3B3 D3B4 D3B5 D3B6 D3B7 D3B8 D4B1 D4B2 D4B3 D4B4 D4B5 D4B6 D4B7 D4B8 D5B1 D5B2 D5B3 D5B4 D5B5 D5B6 D5B7 D5B8 D6B1 D6B2 D6B3 D6B4 D6B5 D6B6 D6B7 D6B8 D7B1 D7B2 D7B3 D7B4 D7B5 D7B6 D7B7 D7B8 D8B1 D8B2 D8B3 D8B4 D8B5 D8B6 D8B7 D8B8 E1B1 E1B2 E1B3 E1B4 E1B5 E1B6 E1B7 E1B8 E2B1 E2B2 E2B3 E2B4 E2B5 E2B6 E2B7 E2B8 E3B1 E3B2 E3B3 E3B4 E3B5 E3B6 E3B7 E3B8 E4B1 E4B2 E4B3 E4B4 E4B5 E4B6 E4B7 E4B8 E5B1 E5B2 E5B3 E5B4 E5B5 E5B6 E5B7 E5B8 E6B1 E6B2 E6B3 E6B4 E6B5 E6B6 E6B7 E6B8 E7B1 E7B2 E7B3 E7B4 E7B5 E7B6 E7B7 E7B8 E8B1 E8B2 E8B3 E8B4 E8B5 E8B6 E8B7 E8B8 F1B1 F1B2 F1B3 F1B4 F1B5 F1B6 F1B7 F1B8 F2B1 F2B2 F2B3 F2B4 F2B5 F2B6 F2B7 F2B8 F3B1 F3B2 F3B3 F3B4 F3B5 F3B6 F3B7 F3B8 F4B1 F4B2 F4B3 F4B4 F4B5 F4B6 F4B7 F4B8 F5B1 F5B2 F5B3 F5B4 F5B5 F5B6 F5B7 F5B8 F6B1 F6B2 F6B3 F6B4 F6B5 F6B6 F6B7 F6B8 F7B1 F7B2 F7B3 F7B4 F7B5 F7B6 F7B7 F7B8 F8B1 F8B2 F8B3 F8B4 F8B5 F8B6 F8B7 F8B8 G1B1 G1B2 G1B3 G1B4 G1B5 G1B6 G1B7 G1B8 G2B1 G2B2 G2B3 G2B4 G2B5 G2B6 G2B7 G2B8 G3B1 G3B2 G3B3 G3B4 G3B5 G3B6 G3B7 G3B8 G4B1 G4B2 G4B3 G4B4 G4B5 G4B6 G4B7 G4B8 G5B1 G5B2 G5B3 G5B4 G5B5 G5B6 G5B7 G5B8 G6B1 G6B2 G6B3 G6B4 G6B5 G6B6 G6B7 G6B8 G7B1 G7B2 G7B3 G7B4 G7B5 G7B6 G7B7 G7B8 G8B1 G8B2 G8B3 G8B4 G8B5 G8B6 G8B7 G8B8 H1B1 H1B2 H1B3 H1B4 H1B5 H1B6 H1B7 H1B8 H2B1 H2B2 H2B3 H2B4 H2B5 H2B6 H2B7 H2B8 H3B1 H3B2 H3B3 H3B4 H3B5 H3B6 H3B7 H3B8 H4B1 H4B2 H4B3 H4B4 H4B5 H4B6 H4B7 H4B8 H5B1 H5B2 H5B3 H5B4 H5B5 H5B6 H5B7 H5B8 H6B1 H6B2 H6B3 H6B4 H6B5 H6B6 H6B7 H6B8 H7B1 H7B2 H7B3 H7B4 H7B5 H7B6 H7B7 H7B8 H8B1 H8B2 H8B3 H8B4 H8B5 H8B6 H8B7 H8B8 A1C1 A1C2 A1C3 A1C4 A1C5 A1C6 A1C7 A1C8 A2C1 A2C2 A2C3 A2C4 A2C5 A2C6 A2C7 A2C8 A3C1 A3C2 A3C3 A3C4 A3C5 A3C6 A3C7 A3C8 A4C1 A4C2 A4C3 A4C4 A4C5 A4C6 A4C7 A4C8 A5C1 A5C2 A5C3 A5C4 A5C5 A5C6 A5C7 A5C8 A6C1 A6C2 A6C3 A6C4 A6C5 A6C6 A6C7 A6C8 A7C1 A7C2 A7C3 A7C4 A7C5 A7C6 A7C7 A7C8 A8C1 A8C2 A8C3 A8C4 A8C5 A8C6 A8C7 A8C8 B1C1 B1C2 B1C3 B1C4 B1C5 B1C6 B1C7 B1C8 B2C1 B2C2 B2C3 B2C4 B2C5 B2C6 B2C7 B2C8 B3C1 B3C2 B3C3 B3C4 B3C5 B3C6 B3C7 B3C8 B4C1 B4C2 B4C3 B4C4 B4C5 B4C6 B4C7 B4C8 B5C1 B5C2 B5C3 B5C4 B5C5 B5C6 B5C7 B5C8 B6C1 B6C2 B6C3 B6C4 B6C5 B6C6 B6C7 B6C8 B7C1 B7C2 B7C3 B7C4 B7C5 B7C6 B7C7 B7C8 B8C1 B8C2 B8C3 B8C4 B8C5 B8C6 B8C7 B8C8 C1C1 C1C2 C1C3 C1C4 C1C5 C1C6 C1C7 C1C8 C2C1 C2C2 C2C3 C2C4 C2C5 C2C6 C2C7 C2C8 C3C1 C3C2 C3C3 C3C4 C3C5 C3C6 C3C7 C3C8 C4C1 C4C2 C4C3 C4C4 C4C5 C4C6 C4C7 C4C8 C5C1 C5C2 C5C3 C5C4 C5C5 C5C6 C5C7 C5C8 C6C1 C6C2 C6C3 C6C4 C6C5 C6C6 C6C7 C6C8 C7C1 C7C2 C7C3 C7C4 C7C5 C7C6 C7C7 C7C8 C8C1 C8C2 C8C3 C8C4 C8C5 C8C6 C8C7 C8C8 D1C1 D1C2 D1C3 D1C4 D1C5 D1C6 D1C7 D1C8 D2C1 D2C2 D2C3 D2C4 D2C5 D2C6 D2C7 D2C8 D3C1 D3C2 D3C3 D3C4 D3C5 D3C6 D3C7 D3C8 D4C1 D4C2 D4C3 D4C4 D4C5 D4C6 D4C7 D4C8 D5C1 D5C2 D5C3 D5C4 D5C5 D5C6 D5C7 D5C8 D6C1 D6C2 D6C3 D6C4 D6C5 D6C6 D6C7 D6C8 D7C1 D7C2 D7C3 D7C4 D7C5 D7C6 D7C7 D7C8 D8C1 D8C2 D8C3 D8C4 D8C5 D8C6 D8C7 D8C8 E1C1 E1C2 E1C3 E1C4 E1C5 E1C6 E1C7 E1C8 E2C1 E2C2 E2C3 E2C4 E2C5 E2C6 E2C7 E2C8 E3C1 E3C2 E3C3 E3C4 E3C5 E3C6 E3C7 E3C8 E4C1 E4C2 E4C3 E4C4 E4C5 E4C6 E4C7 E4C8 E5C1 E5C2 E5C3 E5C4 E5C5 E5C6 E5C7 E5C8 E6C1 E6C2 E6C3 E6C4 E6C5 E6C6 E6C7 E6C8 E7C1 E7C2 E7C3 E7C4 E7C5 E7C6 E7C7 E7C8 E8C1 E8C2 E8C3 E8C4 E8C5 E8C6 E8C7 E8C8 F1C1 F1C2 F1C3 F1C4 F1C5 F1C6 F1C7 F1C8 F2C1 F2C2 F2C3 F2C4 F2C5 F2C6 F2C7 F2C8 F3C1 F3C2 F3C3 F3C4 F3C5 F3C6 F3C7 F3C8 F4C1 F4C2 F4C3 F4C4 F4C5 F4C6 F4C7 F4C8 F5C1 F5C2 F5C3 F5C4 F5C5 F5C6 F5C7 F5C8 F6C1 F6C2 F6C3 F6C4 F6C5 F6C6 F6C7 F6C8 F7C1 F7C2 F7C3 F7C4 F7C5 F7C6 F7C7 F7C8 F8C1 F8C2 F8C3 F8C4 F8C5 F8C6 F8C7 F8C8 G1C1 G1C2 G1C3 G1C4 G1C5 G1C6 G1C7 G1C8 G2C1 G2C2 G2C3 G2C4 G2C5 G2C6 G2C7 G2C8 G3C1 G3C2 G3C3 G3C4 G3C5 G3C6 G3C7 G3C8 G4C1 G4C2 G4C3 G4C4 G4C5 G4C6 G4C7 G4C8 G5C1 G5C2 G5C3 G5C4 G5C5 G5C6 G5C7 G5C8 G6C1 G6C2 G6C3 G6C4 G6C5 G6C6 G6C7 G6C8 G7C1 G7C2 G7C3 G7C4 G7C5 G7C6 G7C7 G7C8 G8C1 G8C2 G8C3 G8C4 G8C5 G8C6 G8C7 G8C8 H1C1 H1C2 H1C3 H1C4 H1C5 H1C6 H1C7 H1C8 H2C1 H2C2 H2C3 H2C4 H2C5 H2C6 H2C7 H2C8 H3C1 H3C2 H3C3 H3C4 H3C5 H3C6 H3C7 H3C8 H4C1 H4C2 H4C3 H4C4 H4C5 H4C6 H4C7 H4C8 H5C1 H5C2 H5C3 H5C4 H5C5 H5C6 H5C7 H5C8 H6C1 H6C2 H6C3 H6C4 H6C5 H6C6 H6C7 H6C8 H7C1 H7C2 H7C3 H7C4 H7C5 H7C6 H7C7 H7C8 H8C1 H8C2 H8C3 H8C4 H8C5 H8C6 H8C7 H8C8 A1D1 A1D2 A1D3 A1D4 A1D5 A1D6 A1D7 A1D8 A2D1 A2D2 A2D3 A2D4 A2D5 A2D6 A2D7 A2D8 A3D1 A3D2 A3D3 A3D4 A3D5 A3D6 A3D7 A3D8 A4D1 A4D2 A4D3 A4D4 A4D5 A4D6 A4D7 A4D8 A5D1 A5D2 A5D3 A5D4 A5D5 A5D6 A5D7 A5D8 A6D1 A6D2 A6D3 A6D4 A6D5 A6D6 A6D7 A6D8 A7D1 A7D2 A7D3 A7D4 A7D5 A7D6 A7D7 A7D8 A8D1 A8D2 A8D3 A8D4 A8D5 A8D6 A8D7 A8D8 B1D1 B1D2 B1D3 B1D4 B1D5 B1D6 B1D7 B1D8 B2D1 B2D2 B2D3 B2D4 B2D5 B2D6 B2D7 B2D8 B3D1 B3D2 B3D3 B3D4 B3D5 B3D6 B3D7 B3D8 B4D1 B4D2 B4D3 B4D4 B4D5 B4D6 B4D7 B4D8 B5D1 B5D2 B5D3 B5D4 B5D5 B5D6 B5D7 B5D8 B6D1 B6D2 B6D3 B6D4 B6D5 B6D6 B6D7 B6D8 B7D1 B7D2 B7D3 B7D4 B7D5 B7D6 B7D7 B7D8 B8D1 B8D2 B8D3 B8D4 B8D5 B8D6 B8D7 B8D8 C1D1 C1D2 C1D3 C1D4 C1D5 C1D6 C1D7 C1D8 C2D1 C2D2 C2D3 C2D4 C2D5 C2D6 C2D7 C2D8 C3D1 C3D2 C3D3 C3D4 C3D5 C3D6 C3D7 C3D8 C4D1 C4D2 C4D3 C4D4 C4D5 C4D6 C4D7 C4D8 C5D1 C5D2 C5D3 C5D4 C5D5 C5D6 C5D7 C5D8 C6D1 C6D2 C6D3 C6D4 C6D5 C6D6 C6D7 C6D8 C7D1 C7D2 C7D3 C7D4 C7D5 C7D6 C7D7 C7D8 C8D1 C8D2 C8D3 C8D4 C8D5 C8D6 C8D7 C8D8 D1D1 D1D2 D1D3 D1D4 D1D5 D1D6 D1D7 D1D8 D2D1 D2D2 D2D3 D2D4 D2D5 D2D6 D2D7 D2D8 D3D1 D3D2 D3D3 D3D4 D3D5 D3D6 D3D7 D3D8 D4D1 D4D2 D4D3 D4D4 D4D5 D4D6 D4D7 D4D8 D5D1 D5D2 D5D3 D5D4 D5D5 D5D6 D5D7 D5D8 D6D1 D6D2 D6D3 D6D4 D6D5 D6D6 D6D7 D6D8 D7D1 D7D2 D7D3 D7D4 D7D5 D7D6 D7D7 D7D8 D8D1 D8D2 D8D3 D8D4 D8D5 D8D6 D8D7 D8D8 E1D1 E1D2 E1D3 E1D4 E1D5 E1D6 E1D7 E1D8 E2D1 E2D2 E2D3 E2D4 E2D5 E2D6 E2D7 E2D8 E3D1 E3D2 E3D3 E3D4 E3D5 E3D6 E3D7 E3D8 E4D1 E4D2 E4D3 E4D4 E4D5 E4D6 E4D7 E4D8 E5D1 E5D2 E5D3 E5D4 E5D5 E5D6 E5D7 E5D8 E6D1 E6D2 E6D3 E6D4 E6D5 E6D6 E6D7 E6D8 E7D1 E7D2 E7D3 E7D4 E7D5 E7D6 E7D7 E7D8 E8D1 E8D2 E8D3 E8D4 E8D5 E8D6 E8D7 E8D8 F1D1 F1D2 F1D3 F1D4 F1D5 F1D6 F1D7 F1D8 F2D1 F2D2 F2D3 F2D4 F2D5 F2D6 F2D7 F2D8 F3D1 F3D2 F3D3 F3D4 F3D5 F3D6 F3D7 F3D8 F4D1 F4D2 F4D3 F4D4 F4D5 F4D6 F4D7 F4D8 F5D1 F5D2 F5D3 F5D4 F5D5 F5D6 F5D7 F5D8 F6D1 F6D2 F6D3 F6D4 F6D5 F6D6 F6D7 F6D8 F7D1 F7D2 F7D3 F7D4 F7D5 F7D6 F7D7 F7D8 F8D1 F8D2 F8D3 F8D4 F8D5 F8D6 F8D7 F8D8 G1D1 G1D2 G1D3 G1D4 G1D5 G1D6 G1D7 G1D8 G2D1 G2D2 G2D3 G2D4 G2D5 G2D6 G2D7 G2D8 G3D1 G3D2 G3D3 G3D4 G3D5 G3D6 G3D7 G3D8 G4D1 G4D2 G4D3 G4D4 G4D5 G4D6 G4D7 G4D8 G5D1 G5D2 G5D3 G5D4 G5D5 G5D6 G5D7 G5D8 G6D1 G6D2 G6D3 G6D4 G6D5 G6D6 G6D7 G6D8 G7D1 G7D2 G7D3 G7D4 G7D5 G7D6 G7D7 G7D8 G8D1 G8D2 G8D3 G8D4 G8D5 G8D6 G8D7 G8D8 H1D1 H1D2 H1D3 H1D4 H1D5 H1D6 H1D7 H1D8 H2D1 H2D2 H2D3 H2D4 H2D5 H2D6 H2D7 H2D8 H3D1 H3D2 H3D3 H3D4 H3D5 H3D6 H3D7 H3D8 H4D1 H4D2 H4D3 H4D4 H4D5 H4D6 H4D7 H4D8 H5D1 H5D2 H5D3 H5D4 H5D5 H5D6 H5D7 H5D8 H6D1 H6D2 H6D3 H6D4 H6D5 H6D6 H6D7 H6D8 H7D1 H7D2 H7D3 H7D4 H7D5 H7D6 H7D7 H7D8 H8D1 H8D2 H8D3 H8D4 H8D5 H8D6 H8D7 H8D8 A1E1 A1E2 A1E3 A1E4 A1E5 A1E6 A1E7 A1E8 A2E1 A2E2 A2E3 A2E4 A2E5 A2E6 A2E7 A2E8 A3E1 A3E2 A3E3 A3E4 A3E5 A3E6 A3E7 A3E8 A4E1 A4E2 A4E3 A4E4 A4E5 A4E6 A4E7 A4E8 A5E1 A5E2 A5E3 A5E4 A5E5 A5E6 A5E7 A5E8 A6E1 A6E2 A6E3 A6E4 A6E5 A6E6 A6E7 A6E8 A7E1 A7E2 A7E3 A7E4 A7E5 A7E6 A7E7 A7E8 A8E1 A8E2 A8E3 A8E4 A8E5 A8E6 A8E7 A8E8 B1E1 B1E2 B1E3 B1E4 B1E5 B1E6 B1E7 B1E8 B2E1 B2E2 B2E3 B2E4 B2E5 B2E6 B2E7 B2E8 B3E1 B3E2 B3E3 B3E4 B3E5 B3E6 B3E7 B3E8 B4E1 B4E2 B4E3 B4E4 B4E5 B4E6 B4E7 B4E8 B5E1 B5E2 B5E3 B5E4 B5E5 B5E6 B5E7 B5E8 B6E1 B6E2 B6E3 B6E4 B6E5 B6E6 B6E7 B6E8 B7E1 B7E2 B7E3 B7E4 B7E5 B7E6 B7E7 B7E8 B8E1 B8E2 B8E3 B8E4 B8E5 B8E6 B8E7 B8E8 C1E1 C1E2 C1E3 C1E4 C1E5 C1E6 C1E7 C1E8 C2E1 C2E2 C2E3 C2E4 C2E5 C2E6 C2E7 C2E8 C3E1 C3E2 C3E3 C3E4 C3E5 C3E6 C3E7 C3E8 C4E1 C4E2 C4E3 C4E4 C4E5 C4E6 C4E7 C4E8 C5E1 C5E2 C5E3 C5E4 C5E5 C5E6 C5E7 C5E8 C6E1 C6E2 C6E3 C6E4 C6E5 C6E6 C6E7 C6E8 C7E1 C7E2 C7E3 C7E4 C7E5 C7E6 C7E7 C7E8 C8E1 C8E2 C8E3 C8E4 C8E5 C8E6 C8E7 C8E8 D1E1 D1E2 D1E3 D1E4 D1E5 D1E6 D1E7 D1E8 D2E1 D2E2 D2E3 D2E4 D2E5 D2E6 D2E7 D2E8 D3E1 D3E2 D3E3 D3E4 D3E5 D3E6 D3E7 D3E8 D4E1 D4E2 D4E3 D4E4 D4E5 D4E6 D4E7 D4E8 D5E1 D5E2 D5E3 D5E4 D5E5 D5E6 D5E7 D5E8 D6E1 D6E2 D6E3 D6E4 D6E5 D6E6 D6E7 D6E8 D7E1 D7E2 D7E3 D7E4 D7E5 D7E6 D7E7 D7E8 D8E1 D8E2 D8E3 D8E4 D8E5 D8E6 D8E7 D8E8 E1E1 E1E2 E1E3 E1E4 E1E5 E1E6 E1E7 E1E8 E2E1 E2E2 E2E3 E2E4 E2E5 E2E6 E2E7 E2E8 E3E1 E3E2 E3E3 E3E4 E3E5 E3E6 E3E7 E3E8 E4E1 E4E2 E4E3 E4E4 E4E5 E4E6 E4E7 E4E8 E5E1 E5E2 E5E3 E5E4 E5E5 E5E6 E5E7 E5E8 E6E1 E6E2 E6E3 E6E4 E6E5 E6E6 E6E7 E6E8 E7E1 E7E2 E7E3 E7E4 E7E5 E7E6 E7E7 E7E8 E8E1 E8E2 E8E3 E8E4 E8E5 E8E6 E8E7 E8E8 F1E1 F1E2 F1E3 F1E4 F1E5 F1E6 F1E7 F1E8 F2E1 F2E2 F2E3 F2E4 F2E5 F2E6 F2E7 F2E8 F3E1 F3E2 F3E3 F3E4 F3E5 F3E6 F3E7 F3E8 F4E1 F4E2 F4E3 F4E4 F4E5 F4E6 F4E7 F4E8 F5E1 F5E2 F5E3 F5E4 F5E5 F5E6 F5E7 F5E8 F6E1 F6E2 F6E3 F6E4 F6E5 F6E6 F6E7 F6E8 F7E1 F7E2 F7E3 F7E4 F7E5 F7E6 F7E7 F7E8 F8E1 F8E2 F8E3 F8E4 F8E5 F8E6 F8E7 F8E8 G1E1 G1E2 G1E3 G1E4 G1E5 G1E6 G1E7 G1E8 G2E1 G2E2 G2E3 G2E4 G2E5 G2E6 G2E7 G2E8 G3E1 G3E2 G3E3 G3E4 G3E5 G3E6 G3E7 G3E8 G4E1 G4E2 G4E3 G4E4 G4E5 G4E6 G4E7 G4E8 G5E1 G5E2 G5E3 G5E4 G5E5 G5E6 G5E7 G5E8 G6E1 G6E2 G6E3 G6E4 G6E5 G6E6 G6E7 G6E8 G7E1 G7E2 G7E3 G7E4 G7E5 G7E6 G7E7 G7E8 G8E1 G8E2 G8E3 G8E4 G8E5 G8E6 G8E7 G8E8 H1E1 H1E2 H1E3 H1E4 H1E5 H1E6 H1E7 H1E8 H2E1 H2E2 H2E3 H2E4 H2E5 H2E6 H2E7 H2E8 H3E1 H3E2 H3E3 H3E4 H3E5 H3E6 H3E7 H3E8 H4E1 H4E2 H4E3 H4E4 H4E5 H4E6 H4E7 H4E8 H5E1 H5E2 H5E3 H5E4 H5E5 H5E6 H5E7 H5E8 H6E1 H6E2 H6E3 H6E4 H6E5 H6E6 H6E7 H6E8 H7E1 H7E2 H7E3 H7E4 H7E5 H7E6 H7E7 H7E8 H8E1 H8E2 H8E3 H8E4 H8E5 H8E6 H8E7 H8E8 A1F1 A1F2 A1F3 A1F4 A1F5 A1F6 A1F7 A1F8 A2F1 A2F2 A2F3 A2F4 A2F5 A2F6 A2F7 A2F8 A3F1 A3F2 A3F3 A3F4 A3F5 A3F6 A3F7 A3F8 A4F1 A4F2 A4F3 A4F4 A4F5 A4F6 A4F7 A4F8 A5F1 A5F2 A5F3 A5F4 A5F5 A5F6 A5F7 A5F8 A6F1 A6F2 A6F3 A6F4 A6F5 A6F6 A6F7 A6F8 A7F1 A7F2 A7F3 A7F4 A7F5 A7F6 A7F7 A7F8 A8F1 A8F2 A8F3 A8F4 A8F5 A8F6 A8F7 A8F8 B1F1 B1F2 B1F3 B1F4 B1F5 B1F6 B1F7 B1F8 B2F1 B2F2 B2F3 B2F4 B2F5 B2F6 B2F7 B2F8 B3F1 B3F2 B3F3 B3F4 B3F5 B3F6 B3F7 B3F8 B4F1 B4F2 B4F3 B4F4 B4F5 B4F6 B4F7 B4F8 B5F1 B5F2 B5F3 B5F4 B5F5 B5F6 B5F7 B5F8 B6F1 B6F2 B6F3 B6F4 B6F5 B6F6 B6F7 B6F8 B7F1 B7F2 B7F3 B7F4 B7F5 B7F6 B7F7 B7F8 B8F1 B8F2 B8F3 B8F4 B8F5 B8F6 B8F7 B8F8 C1F1 C1F2 C1F3 C1F4 C1F5 C1F6 C1F7 C1F8 C2F1 C2F2 C2F3 C2F4 C2F5 C2F6 C2F7 C2F8 C3F1 C3F2 C3F3 C3F4 C3F5 C3F6 C3F7 C3F8 C4F1 C4F2 C4F3 C4F4 C4F5 C4F6 C4F7 C4F8 C5F1 C5F2 C5F3 C5F4 C5F5 C5F6 C5F7 C5F8 C6F1 C6F2 C6F3 C6F4 C6F5 C6F6 C6F7 C6F8 C7F1 C7F2 C7F3 C7F4 C7F5 C7F6 C7F7 C7F8 C8F1 C8F2 C8F3 C8F4 C8F5 C8F6 C8F7 C8F8 D1F1 D1F2 D1F3 D1F4 D1F5 D1F6 D1F7 D1F8 D2F1 D2F2 D2F3 D2F4 D2F5 D2F6 D2F7 D2F8 D3F1 D3F2 D3F3 D3F4 D3F5 D3F6 D3F7 D3F8 D4F1 D4F2 D4F3 D4F4 D4F5 D4F6 D4F7 D4F8 D5F1 D5F2 D5F3 D5F4 D5F5 D5F6 D5F7 D5F8 D6F1 D6F2 D6F3 D6F4 D6F5 D6F6 D6F7 D6F8 D7F1 D7F2 D7F3 D7F4 D7F5 D7F6 D7F7 D7F8 D8F1 D8F2 D8F3 D8F4 D8F5 D8F6 D8F7 D8F8 E1F1 E1F2 E1F3 E1F4 E1F5 E1F6 E1F7 E1F8 E2F1 E2F2 E2F3 E2F4 E2F5 E2F6 E2F7 E2F8 E3F1 E3F2 E3F3 E3F4 E3F5 E3F6 E3F7 E3F8 E4F1 E4F2 E4F3 E4F4 E4F5 E4F6 E4F7 E4F8 E5F1 E5F2 E5F3 E5F4 E5F5 E5F6 E5F7 E5F8 E6F1 E6F2 E6F3 E6F4 E6F5 E6F6 E6F7 E6F8 E7F1 E7F2 E7F3 E7F4 E7F5 E7F6 E7F7 E7F8 E8F1 E8F2 E8F3 E8F4 E8F5 E8F6 E8F7 E8F8 F1F1 F1F2 F1F3 F1F4 F1F5 F1F6 F1F7 F1F8 F2F1 F2F2 F2F3 F2F4 F2F5 F2F6 F2F7 F2F8 F3F1 F3F2 F3F3 F3F4 F3F5 F3F6 F3F7 F3F8 F4F1 F4F2 F4F3 F4F4 F4F5 F4F6 F4F7 F4F8 F5F1 F5F2 F5F3 F5F4 F5F5 F5F6 F5F7 F5F8 F6F1 F6F2 F6F3 F6F4 F6F5 F6F6 F6F7 F6F8 F7F1 F7F2 F7F3 F7F4 F7F5 F7F6 F7F7 F7F8 F8F1 F8F2 F8F3 F8F4 F8F5 F8F6 F8F7 F8F8 G1F1 G1F2 G1F3 G1F4 G1F5 G1F6 G1F7 G1F8 G2F1 G2F2 G2F3 G2F4 G2F5 G2F6 G2F7 G2F8 G3F1 G3F2 G3F3 G3F4 G3F5 G3F6 G3F7 G3F8 G4F1 G4F2 G4F3 G4F4 G4F5 G4F6 G4F7 G4F8 G5F1 G5F2 G5F3 G5F4 G5F5 G5F6 G5F7 G5F8 G6F1 G6F2 G6F3 G6F4 G6F5 G6F6 G6F7 G6F8 G7F1 G7F2 G7F3 G7F4 G7F5 G7F6 G7F7 G7F8 G8F1 G8F2 G8F3 G8F4 G8F5 G8F6 G8F7 G8F8 H1F1 H1F2 H1F3 H1F4 H1F5 H1F6 H1F7 H1F8 H2F1 H2F2 H2F3 H2F4 H2F5 H2F6 H2F7 H2F8 H3F1 H3F2 H3F3 H3F4 H3F5 H3F6 H3F7 H3F8 H4F1 H4F2 H4F3 H4F4 H4F5 H4F6 H4F7 H4F8 H5F1 H5F2 H5F3 H5F4 H5F5 H5F6 H5F7 H5F8 H6F1 H6F2 H6F3 H6F4 H6F5 H6F6 H6F7 H6F8 H7F1 H7F2 H7F3 H7F4 H7F5 H7F6 H7F7 H7F8 H8F1 H8F2 H8F3 H8F4 H8F5 H8F6 H8F7 H8F8 A1G1 A1G2 A1G3 A1G4 A1G5 A1G6 A1G7 A1G8 A2G1 A2G2 A2G3 A2G4 A2G5 A2G6 A2G7 A2G8 A3G1 A3G2 A3G3 A3G4 A3G5 A3G6 A3G7 A3G8 A4G1 A4G2 A4G3 A4G4 A4G5 A4G6 A4G7 A4G8 A5G1 A5G2 A5G3 A5G4 A5G5 A5G6 A5G7 A5G8 A6G1 A6G2 A6G3 A6G4 A6G5 A6G6 A6G7 A6G8 A7G1 A7G2 A7G3 A7G4 A7G5 A7G6 A7G7 A7G8 A8G1 A8G2 A8G3 A8G4 A8G5 A8G6 A8G7 A8G8 B1G1 B1G2 B1G3 B1G4 B1G5 B1G6 B1G7 B1G8 B2G1 B2G2 B2G3 B2G4 B2G5 B2G6 B2G7 B2G8 B3G1 B3G2 B3G3 B3G4 B3G5 B3G6 B3G7 B3G8 B4G1 B4G2 B4G3 B4G4 B4G5 B4G6 B4G7 B4G8 B5G1 B5G2 B5G3 B5G4 B5G5 B5G6 B5G7 B5G8 B6G1 B6G2 B6G3 B6G4 B6G5 B6G6 B6G7 B6G8 B7G1 B7G2 B7G3 B7G4 B7G5 B7G6 B7G7 B7G8 B8G1 B8G2 B8G3 B8G4 B8G5 B8G6 B8G7 B8G8 C1G1 C1G2 C1G3 C1G4 C1G5 C1G6 C1G7 C1G8 C2G1 C2G2 C2G3 C2G4 C2G5 C2G6 C2G7 C2G8 C3G1 C3G2 C3G3 C3G4 C3G5 C3G6 C3G7 C3G8 C4G1 C4G2 C4G3 C4G4 C4G5 C4G6 C4G7 C4G8 C5G1 C5G2 C5G3 C5G4 C5G5 C5G6 C5G7 C5G8 C6G1 C6G2 C6G3 C6G4 C6G5 C6G6 C6G7 C6G8 C7G1 C7G2 C7G3 C7G4 C7G5 C7G6 C7G7 C7G8 C8G1 C8G2 C8G3 C8G4 C8G5 C8G6 C8G7 C8G8 D1G1 D1G2 D1G3 D1G4 D1G5 D1G6 D1G7 D1G8 D2G1 D2G2 D2G3 D2G4 D2G5 D2G6 D2G7 D2G8 D3G1 D3G2 D3G3 D3G4 D3G5 D3G6 D3G7 D3G8 D4G1 D4G2 D4G3 D4G4 D4G5 D4G6 D4G7 D4G8 D5G1 D5G2 D5G3 D5G4 D5G5 D5G6 D5G7 D5G8 D6G1 D6G2 D6G3 D6G4 D6G5 D6G6 D6G7 D6G8 D7G1 D7G2 D7G3 D7G4 D7G5 D7G6 D7G7 D7G8 D8G1 D8G2 D8G3 D8G4 D8G5 D8G6 D8G7 D8G8 E1G1 E1G2 E1G3 E1G4 E1G5 E1G6 E1G7 E1G8 E2G1 E2G2 E2G3 E2G4 E2G5 E2G6 E2G7 E2G8 E3G1 E3G2 E3G3 E3G4 E3G5 E3G6 E3G7 E3G8 E4G1 E4G2 E4G3 E4G4 E4G5 E4G6 E4G7 E4G8 E5G1 E5G2 E5G3 E5G4 E5G5 E5G6 E5G7 E5G8 E6G1 E6G2 E6G3 E6G4 E6G5 E6G6 E6G7 E6G8 E7G1 E7G2 E7G3 E7G4 E7G5 E7G6 E7G7 E7G8 E8G1 E8G2 E8G3 E8G4 E8G5 E8G6 E8G7 E8G8 F1G1 F1G2 F1G3 F1G4 F1G5 F1G6 F1G7 F1G8 F2G1 F2G2 F2G3 F2G4 F2G5 F2G6 F2G7 F2G8 F3G1 F3G2 F3G3 F3G4 F3G5 F3G6 F3G7 F3G8 F4G1 F4G2 F4G3 F4G4 F4G5 F4G6 F4G7 F4G8 F5G1 F5G2 F5G3 F5G4 F5G5 F5G6 F5G7 F5G8 F6G1 F6G2 F6G3 F6G4 F6G5 F6G6 F6G7 F6G8 F7G1 F7G2 F7G3 F7G4 F7G5 F7G6 F7G7 F7G8 F8G1 F8G2 F8G3 F8G4 F8G5 F8G6 F8G7 F8G8 G1G1 G1G2 G1G3 G1G4 G1G5 G1G6 G1G7 G1G8 G2G1 G2G2 G2G3 G2G4 G2G5 G2G6 G2G7 G2G8 G3G1 G3G2 G3G3 G3G4 G3G5 G3G6 G3G7 G3G8 G4G1 G4G2 G4G3 G4G4 G4G5 G4G6 G4G7 G4G8 G5G1 G5G2 G5G3 G5G4 G5G5 G5G6 G5G7 G5G8 G6G1 G6G2 G6G3 G6G4 G6G5 G6G6 G6G7 G6G8 G7G1 G7G2 G7G3 G7G4 G7G5 G7G6 G7G7 G7G8 G8G1 G8G2 G8G3 G8G4 G8G5 G8G6 G8G7 G8G8 H1G1 H1G2 H1G3 H1G4 H1G5 H1G6 H1G7 H1G8 H2G1 H2G2 H2G3 H2G4 H2G5 H2G6 H2G7 H2G8 H3G1 H3G2 H3G3 H3G4 H3G5 H3G6 H3G7 H3G8 H4G1 H4G2 H4G3 H4G4 H4G5 H4G6 H4G7 H4G8 H5G1 H5G2 H5G3 H5G4 H5G5 H5G6 H5G7 H5G8 H6G1 H6G2 H6G3 H6G4 H6G5 H6G6 H6G7 H6G8 H7G1 H7G2 H7G3 H7G4 H7G5 H7G6 H7G7 H7G8 H8G1 H8G2 H8G3 H8G4 H8G5 H8G6 H8G7 H8G8 A1H1 A1H2 A1H3 A1H4 A1H5 A1H6 A1H7 A1H8 A2H1 A2H2 A2H3 A2H4 A2H5 A2H6 A2H7 A2H8 A3H1 A3H2 A3H3 A3H4 A3H5 A3H6 A3H7 A3H8 A4H1 A4H2 A4H3 A4H4 A4H5 A4H6 A4H7 A4H8 A5H1 A5H2 A5H3 A5H4 A5H5 A5H6 A5H7 A5H8 A6H1 A6H2 A6H3 A6H4 A6H5 A6H6 A6H7 A6H8 A7H1 A7H2 A7H3 A7H4 A7H5 A7H6 A7H7 A7H8 A8H1 A8H2 A8H3 A8H4 A8H5 A8H6 A8H7 A8H8 B1H1 B1H2 B1H3 B1H4 B1H5 B1H6 B1H7 B1H8 B2H1 B2H2 B2H3 B2H4 B2H5 B2H6 B2H7 B2H8 B3H1 B3H2 B3H3 B3H4 B3H5 B3H6 B3H7 B3H8 B4H1 B4H2 B4H3 B4H4 B4H5 B4H6 B4H7 B4H8 B5H1 B5H2 B5H3 B5H4 B5H5 B5H6 B5H7 B5H8 B6H1 B6H2 B6H3 B6H4 B6H5 B6H6 B6H7 B6H8 B7H1 B7H2 B7H3 B7H4 B7H5 B7H6 B7H7 B7H8 B8H1 B8H2 B8H3 B8H4 B8H5 B8H6 B8H7 B8H8 C1H1 C1H2 C1H3 C1H4 C1H5 C1H6 C1H7 C1H8 C2H1 C2H2 C2H3 C2H4 C2H5 C2H6 C2H7 C2H8 C3H1 C3H2 C3H3 C3H4 C3H5 C3H6 C3H7 C3H8 C4H1 C4H2 C4H3 C4H4 C4H5 C4H6 C4H7 C4H8 C5H1 C5H2 C5H3 C5H4 C5H5 C5H6 C5H7 C5H8 C6H1 C6H2 C6H3 C6H4 C6H5 C6H6 C6H7 C6H8 C7H1 C7H2 C7H3 C7H4 C7H5 C7H6 C7H7 C7H8 C8H1 C8H2 C8H3 C8H4 C8H5 C8H6 C8H7 C8H8 D1H1 D1H2 D1H3 D1H4 D1H5 D1H6 D1H7 D1H8 D2H1 D2H2 D2H3 D2H4 D2H5 D2H6 D2H7 D2H8 D3H1 D3H2 D3H3 D3H4 D3H5 D3H6 D3H7 D3H8 D4H1 D4H2 D4H3 D4H4 D4H5 D4H6 D4H7 D4H8 D5H1 D5H2 D5H3 D5H4 D5H5 D5H6 D5H7 D5H8 D6H1 D6H2 D6H3 D6H4 D6H5 D6H6 D6H7 D6H8 D7H1 D7H2 D7H3 D7H4 D7H5 D7H6 D7H7 D7H8 D8H1 D8H2 D8H3 D8H4 D8H5 D8H6 D8H7 D8H8 E1H1 E1H2 E1H3 E1H4 E1H5 E1H6 E1H7 E1H8 E2H1 E2H2 E2H3 E2H4 E2H5 E2H6 E2H7 E2H8 E3H1 E3H2 E3H3 E3H4 E3H5 E3H6 E3H7 E3H8 E4H1 E4H2 E4H3 E4H4 E4H5 E4H6 E4H7 E4H8 E5H1 E5H2 E5H3 E5H4 E5H5 E5H6 E5H7 E5H8 E6H1 E6H2 E6H3 E6H4 E6H5 E6H6 E6H7 E6H8 E7H1 E7H2 E7H3 E7H4 E7H5 E7H6 E7H7 E7H8 E8H1 E8H2 E8H3 E8H4 E8H5 E8H6 E8H7 E8H8 F1H1 F1H2 F1H3 F1H4 F1H5 F1H6 F1H7 F1H8 F2H1 F2H2 F2H3 F2H4 F2H5 F2H6 F2H7 F2H8 F3H1 F3H2 F3H3 F3H4 F3H5 F3H6 F3H7 F3H8 F4H1 F4H2 F4H3 F4H4 F4H5 F4H6 F4H7 F4H8 F5H1 F5H2 F5H3 F5H4 F5H5 F5H6 F5H7 F5H8 F6H1 F6H2 F6H3 F6H4 F6H5 F6H6 F6H7 F6H8 F7H1 F7H2 F7H3 F7H4 F7H5 F7H6 F7H7 F7H8 F8H1 F8H2 F8H3 F8H4 F8H5 F8H6 F8H7 F8H8 G1H1 G1H2 G1H3 G1H4 G1H5 G1H6 G1H7 G1H8 G2H1 G2H2 G2H3 G2H4 G2H5 G2H6 G2H7 G2H8 G3H1 G3H2 G3H3 G3H4 G3H5 G3H6 G3H7 G3H8 G4H1 G4H2 G4H3 G4H4 G4H5 G4H6 G4H7 G4H8 G5H1 G5H2 G5H3 G5H4 G5H5 G5H6 G5H7 G5H8 G6H1 G6H2 G6H3 G6H4 G6H5 G6H6 G6H7 G6H8 G7H1 G7H2 G7H3 G7H4 G7H5 G7H6 G7H7 G7H8 G8H1 G8H2 G8H3 G8H4 G8H5 G8H6 G8H7 G8H8 H1H1 H1H2 H1H3 H1H4 H1H5 H1H6 H1H7 H1H8 H2H1 H2H2 H2H3 H2H4 H2H5 H2H6 H2H7 H2H8 H3H1 H3H2 H3H3 H3H4 H3H5 H3H6 H3H7 H3H8 H4H1 H4H2 H4H3 H4H4 H4H5 H4H6 H4H7 H4H8 H5H1 H5H2 H5H3 H5H4 H5H5 H5H6 H5H7 H5H8 H6H1 H6H2 H6H3 H6H4 H6H5 H6H6 H6H7 H6H8 H7H1 H7H2 H7H3 H7H4 H7H5 H7H6 H7H7 H7H8 H8H1 H8H2 H8H3 H8H4 H8H5 H8H6 H8H7 H8H8

The above-cited polypeptides can further comprise one or more sequences selected from the group consisting of SEQ ID NO: 81 to 89.

There is also provided a composition comprising a plurality of identical or different polypeptides as defined herein above and a physiologically acceptable carrier. The composition can be a dried composition and the dried composition can be in freeze dried form or spray dried form.

In an even further aspect the present invention is directed to a polypeptide having an ice-binding activity and being capable of reducing or inhibiting the growth and/or formation of ice-crystals, wherein the polypeptide comprises the sequence X₁-X₂-X₃-X₄-X₅-X₆-X₇-X₈-X₉ (SEQ ID NO:90),

wherein X₁ is selected from the group of amino acid residues consisting of S, A, G and D;

X₂ is selected from the group of amino acid residues consisting of A, V, I, T and S;

X₃ is selected from the group of amino acid residues consisting of non-bulky amino acid residues;

X₄ is selected from the group of amino acid residues consisting of S, I, T and V;

X₅ is selected from the group of amino acid residues consisting of S, A, I and T;

X₆ is selected from the group of amino acid residues consisting of S, T and V;

X₇ is selected from the group of amino acid residues consisting of non-bulky amino acid residues;

X₈ is selected from the group of amino acid residues consisting of S, T and V;

X₉ is selected from the group of amino acid residues consisting of S, A and G; and

wherein at least one of the residues X₂, X₄, X₆ and X₈ of SEQ ID NO:1 is T or V; and

wherein the total number of amino acid residues of the polypeptide is less than 250.

The sequence X₁-X₂-X₃-X₄-X₅-X₆-X₇-X₈-X₉ (SEQ ID NO:90) will be referred to herein as a general “ice-binding domain”. Whether or not said domain is directly involved in ice-binding or only indirectly involved in ice-binding (i.e. is required in order for the polypeptide to have an ice-binding activity) is immaterial to this definition.

The invention further relates to polypeptides comprising a plurality of general “ice-binding domains”, such as sequences having a substantial homology/identity to SEQ ID NO:90. A substantial homology to SEQ ID NO:90 shall in this respect encompass any sequence, which differs from the sequence of SEQ ID NO:90 in only one or at the most two of the positions X₁, X₂, X₃, X₄, X₅, X₆ , X₇, X₈ and X₉.

Plurality as used in this respect shall encompass the intergers 2, 3, 4, 5, 6, 7, 8, 8, 9, such as less than 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, for example less than 25, wherein any of said plurality of general “ice-binding domains” can be identical to or substantially identical to or substantially homologous to SEQ ID NO:90.

The invention also relates to modifications and derivatives of the polypeptide according to the present invention comprising one or more copies of SEQ ID NO:90 and optionally further comprising one or more copies of SEQ ID NO:90, or sequences substantially identical or substantially homologous thereto.

The term “isolated polypeptide” clarifies that the polypeptide according to the present invention is at least essentially free from contaminating cellular components natively associated with the polypeptide.

The polypeptides according to the present invention can be expressed as fusion polypeptides. Such fusion polypeptides can serve many functions, such as aiding in purification and/or production of the polypeptide in an active conformation. One example of a fusion polypeptide is a polypeptide according to the present invention fused to an affinity tag. The fusion polypeptides can also form part of a complement/anti-complement pair as defined herein, although this term is not limited exclusively to polypeptides. Modifications of the polypeptides, such as splice variants, allelic variants, orthologs and paralogs as defined herein are also within the scope of the present invention. Examples of fusions polypeptides are disclosed herein below in more detail.

The polypeptides according to the present invention can be labelled with a detactable label, for example a fluorescently detactable label. This can help the practitioner in isolating or identifying the polypeptides according to the present invention.

Also provided are polynucleotides encoding the polypeptides according to the present invention, polynucleotide constructs, such as vectors comprising said nucleotides in linear or circular form, host cells transformed with said polynucleotides or vectors comprising said nucleotides, and transgenic organisms comprising said host cells.

Polynucleotide is used interchangably with “nucleic acid” and “nucleic acid molecule” unless otherwise indicated. In principle the invention can make use of such a polynucleotide or a complement of such a polynucleotide, for example in the form of a cDNA or an “anti-sense oligonucleotide”. The polynucleotide can be a degenerated sequence of individual nucleotides as long as the polynucleotide encodes a polypeptide according to the present invention.

The polynucleotide need not comprise all of the individual nucleotides of a native gene as isolated from a natural host organism. Any truncation of such as native gene, including sequences which are at least 75% homologous or identical, such as at least 80% homologous or identical, for example at least 85% homologous or identical, such as at least 90% homologous or identical, for example at least 91% homologous or identical, such as at least 92% homologous or identical, for example at least 93% homologous or identical, such as at least 94% homologous or identical, for example at least 95% homologous or identical, such as at least 96% homologous or identical, for example at least 97% homologous or identical, such as at least 98% homologous or identical, for example at least 99% homologous or identical, such as at least 99.5% homologous or identical to the native gene shall be encompassed by the present invention. Any such functional truncation of a native gene, including any derivative or modification thereof capable of being expressed in a suitable host organism, shall be denoted a “structural gene”.

Expression can be obtained e.g. when a polynucleotide sequence cloned in a cloning vector or expression vector is introduced into a host organism and expressed. The expression is suitably directed by a regulatory sequence typically comprising a promoter which may again comprising elements such as a core promoter and one or more regulatory elements, including an enhancer of expression.

The host organism will typically be a recombinant host, i.e. a host not natively harbouring the polynucleotide sequence to be expressed, or not natively comprising the polynucleotide sequence to be expressed operably linked to the native expression signal. When preceded by a secretory signal sequence the polypeptide according to the present invention is destined for secretion—irrespective of whether such a secretion takes place or not.

For the sake of clarity a polynucleotide according to the present invention will be referred to as an “isolated” polynucleotide in order to distinguish the polynucleotide from the same or a related sequence in its native environment. Likewise, the term “heterologous polynucleotide” as defined herein signifies a polynucleotide or polynucleotide construct which differs from the native form of the polypeptide according to the present invention. The polynucleotides according to the present invention can be chromosomally integrated or episomal.

The invention also provides antibodies, or binding fragments thereof, specific for the polypeptides according to the invention. The antibodies can be produced by any state-of-the-art method and the antibodies can be e.g. a naked antibody, a binding fragment of an antibody, an antibody component, an anti-idiotype antibody, a chimeric antibody and a humanized antibody.

Also provided with the scope of the present invention are methods for producing and using both a) polynucleotides according to the present invention, b) polypeptides according to the present invention, and c) antibodies according to the present invention specific for said polypeptides. The antibodies according to the present invention can be used for identifying or partitioning from a population of polypeptides a “target polypeptide” or “target peptide” as deined herein. The “target peptide” can be an antigenic peptide” as defined herein.

In a further aspect there is provided a solid support comprising the polypeptides and/or the antibodies according to the present invention as well as methods for making and using such a solid support.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 illustrates the amino acid sequence of a fragment of a R. Inquisitor AFP. Putative ice binding motifs are indicated by black boxes. The forward (F) and reverse (R) primers are given by sequence, and the parts of the polypeptide they are derived from is also indicated. The amino acid sequence has a domain which represents or harbours an ice-binding (IB) motif (consensus A/G-Q/R-G-T-A-T-T-T-A-T-G-X-A/G) repeated X times and separated by 1-8 amino acid residues.

FIG. 2 illustrates the full-length cDNA sequences of AFPs 1-8 from R. mordax. Coding sequences for putative signal peptides are underlined. The AFP's corresponding to these cDNAs (AFP 1-8) mainly differed in their N-terminal part, where AFP4 would appear to be without a signal sequence. The AFPs each contained 8 repeats of an amino acid sequence with the core concensus sequence T-A-T-T-T-A-T. This matches part of the putative IB-motif also observed for R. inquisitor (FIG. 1), and it is conceivable that this represents the IB motif of R. mordax or at least part of it.

FIG. 3. Full length amino acid sequences of AFPs 1-8 from Rhagium mordax. These sequences are denoted SEQ ID NO:1 to SEQ ID NO:8, respectively. Putative signal sequences are indicated in underscore and putative ice binding motives are indicated in boxes.

FIG. 4. Illustration of E. coli cells harbouring full length cDNA's cloned into the expression vector pET26B(+). IPTG can be used to induce synthesis of the pertinent AFP. Initially we have induced synthesis of AFP1 in E. coli. The results demonstrate that AFP1 produced in E. coli confer on the E. coli cells the ability to withstand freezing.

FIG. 5. Illustrates an SDS-PAGE gel of wild type (wt) soform RmAFP1 and deletion variants (Δ 2-9, WC (Trp-Cys)). The RmAFP variants were constructed as deletions of the ice binding domains one at a time from the C-terminal of RmAFP1 (Δ2-Δ9) (for example Δ4 indicates that putative ice binding domain 4-9 (see FIG. 2) has been deleted) and a variant containing a Trp and Cys residue in the C-terminal (WC). These were cloned into pGEX vector system (GE Healthcare) and transformed in E. coli (strains: Origami, BL21). The proteins were expressed as a fusion protein to Glutathione-S-transferase (GST) containing a thrombin cleavage site between GST and RmAFP.

FIG. 6. Shows the progression of the “ice growth-explosion” which occurs at the hysteresis freezing point in 1) a solution of RmAFP1 and 2) in serum of the eel pout Zoarces viviparus (a danish fish with type 3 AFP). In both cases a small ice crystal was allowed to anneal in the solution before the temperature was lowered. When decreasing the temperature in the RmAFP solution the initial ice crystal (at the arrow in 1A) does not grow or change shape before the hysteresis freezing point is reached. At the hysteresis freezing point (1B) the ice crystal bursts and due to the super cooled surroundings the solution freezes. Note that in this case the ice growth pattern at the hysteresis freezing point is cauliflower like (1B, 1C). This is in contrast to the events seen in 2) (the Z. viviparus serum) where, upon cooling, the ice crystal slowly changes shape and become a bipyramid with a hexagonal base plane (2A). At the hysteresis freezing point the ice growth is spicular (2B) and all ice in the solution is growing as spicules (2C).

FIG. 7. Shows the molecular weight determination of cloned and purified Rhagium mordax AFP 1.

FIG. 8. Shows dimerization of rRmAFP1. A: Under native conditions rRmAFP behaves as a dimer, since when it is passed over a size exclusion column (Superdex 75, 10/30, GE Healthcare) it has shorter retention times than other proteins in the same Mw range. Mw estimation from SEC of rRmAFP1 gave 28 KDa when the Superdex 75 was calibrated with bovine serum albumin (68 KDa, BSA), trypsin (25 KDa), and human cystatin C (13 KDa). B: Likewise RmAFP behaves as a dimer when run in an SDS PAGE giving an estimated Mw of 28 KDa. Lane 1: Bovine serum albumin (BSA); 2: Trypsin; 3: RNAse A; 4 Cystatin C; 5: Lysozyme; 6 RmAFP1; 7: Glutathione S transferase (GST).

FIG. 9. Shows a circular dichroism spectroscopy (CD) of a polypeptide according to the invention.

FIG. 10. Shows the temperature stability of RmAFP1 within the temperature range 6-100° C. and succeeding 11 scans were performed within the temperature range 6-70° C.

FIG. 11. Show the influence of pH on activity and stability of RmAFP1.

DEFINITIONS

Antifreeze proteins lower the freezing temperature of a solution noncolligatively by binding to ice crystals and inhibiting crystal growth, but the proteins alter the melting temperature of the solution only by colligative effects. This thermal hysteresis (the difference between freezing and melting temperatures) is determined by observing the effect of temperature on the growth of a single ice crystal. Melting occurs when faces of the ice crystal become round; freezing occurs when the ice crystal elongates along its c-axis.

Hence, the term “anti-freeze activity” refers to the separation of the melting and freezing temperature. It also refers to the difference between melting point and the freezing point. It further refers to the inhibition of formation of large crystals at the expense of small crystals at temperatures above the temperature of recrystallisation. The term anti-freeze activity can be used interchangeably with thermal hysteresis.

As used herein, “nucleic acid” or “nucleic acid molecule” refers to polynucleotides, such as deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), oligonucleotides, fragments generated by the polymerase chain reaction (PCR), and fragments generated by any of ligation, scission, endonuclease action, and exonuclease action. polynucleotide molecules can be composed of monomers that are naturally-occurring nucleotides (such as DNA and RNA), or analogs of naturally-occurring nucleotides (e.g., (alpha-enantiomeric forms of naturally-occurring nucleotides), or a combination of both. Modified nucleotides can have alterations in sugar moieties and/or in pyrimidine or purine base moieties. Sugar modifications include, for example, replacement of one or more hydroxyl groups with halogens, alkyl groups, amines, and azido groups, or sugars can be functionalized as ethers or esters. Moreover, the entire sugar moiety can be replaced with sterically and electronically similar structures, such as aza-sugars and carbocyclic sugar analogs. Examples of modifications in a base moiety include alkylated purines and pyrimidines, acylated purines or pyrimidines, or other well-known heterocyclic substitutes. polynucleotide monomers can be linked by phosphodiester bonds or analogs of such linkages. Analogs of phosphodiester linkages include phosphorothioate, phosphorodithioate, phosphoroselenoate, phosphorodiselenoate, phosphoroanilothioate, phosphoranilidate, phosphoramidate, and the like. The term “nucleic acid molecule” also includes so-called “peptide polynucleotides,” which comprise naturally-occurring or modified polynucleotide bases attached to a polyamide backbone. polynucleotides can be either single stranded or double stranded.

The term “complement of a polynucleotide molecule” refers to a polynucleotide molecule having a complementary nucleotide sequence and reverse orientation as compared to a reference nucleotide sequence. For example, the sequence 5′ ATGCACGGG 3′ is complementary to 5′ CCCGTGCAT 3′.

The term “degenerate nucleotide sequence” denotes a sequence of nucleotides that includes one or more degenerate codons as compared to a reference polynucleotide molecule that encodes a polypeptide. Degenerate codons contain different triplets of nucleotides, but encode the same amino acid residue (i.e., GAU and GAC triplets each encode Asp).

The term “structural gene” refers to a polynucleotide molecule that is transcribed into messenger RNA (mRNA), which is then translated into a sequence of amino acids characteristic of a specific polypeptide.

An “isolated polynucleotide molecule” is a polynucleotide molecule that is not integrated in the genomic DNA of an organism. For example, a DNA molecule that encodes a growth factor that has been separated from the genomic DNA of a cell is an isolated DNA molecule. Another example of an isolated polynucleotide molecule is a chemically-synthesized polynucleotide molecule that is not integrated in the genome of an organism. A polynucleotide molecule that has been isolated from a particular species is smaller than the complete DNA molecule of a chromosome from that species.

A “nucleic acid molecule construct” is a polynucleotide molecule, either single- or double-stranded, that has been modified through human intervention to contain segments of polynucleotide combined and juxtaposed in an arrangement not existing in nature.

“Linear DNA” denotes non-circular DNA molecules having free 5′ and 3′ ends. Linear DNA can be prepared from closed circular DNA molecules, such as plasmids, by enzymatic digestion or physical disruption.

“Complementary DNA (cDNA)” is a single-stranded DNA molecule that is formed from an mRNA template by the enzyme reverse transcriptase. Typically, a primer complementary to portions of mRNA is employed for the initiation of reverse transcription. Those skilled in the art also use the term “cDNA” to refer to a double-stranded DNA molecule consisting of such a single-stranded DNA molecule and its complementary DNA strand. The term “cDNA” also refers to a clone of a cDNA molecule synthesized from an RNA template.

A “promoter” is a nucleotide sequence that directs the transcription of a structural gene.

Typically, a promoter is located in the 5′ non-coding region of a gene, proximal to the transcriptional start site of a structural gene. Sequence elements within promoters that function in the initiation of transcription are often characterized by consensus nucleotide sequences. These promoter elements include RNA polymerase binding sites, TATA sequences, CAAT sequences, differentiation-specific elements (DSEs; McGehee et al., Mol. Endocrinol. 7:551 (1993)), cyclic AMP response elements (CREs), serum response elements (SREs; Treisman, Seminars in Cancer Biol. 1:47 (1990)), glucocorticoid response elements (GREs), and binding sites for other transcription factors, such as CRE/ATF (O'Reilly et al., J. Biol. Chem. 267:19938 (1992)), AP2 (Ye et al., J. Biol. Chem. 269:25728 (1994)), SP1, cAMP response element binding polypeptide (CREB; Loeken, Gene Expr. 3:253 (1993)) and octamer factors (see, in general, Watson et al., eds., Molecular Biology of the Gene, 4th ed. (The Benjamin/Cummings Publishing Company, Inc. 1987), and Lemaigre and Rousseau, Biochem. J. 303:1 (1994)). If a promoter is an inducible promoter, then the rate of transcription increases in response to an inducing agent. In contrast, the rate of transcription is not regulated by an inducing agent if the promoter is a constitutive promoter. Repressible promoters are also known.

A “core promoter” contains essential nucleotide sequences for promoter function, including the TATA box and start of transcription. By this definition, a core promoter may or may not have detectable activity in the absence of specific sequences that may enhance the activity or confer tissue specific activity.

A “regulatory element” is a nucleotide sequence that modulates the activity of a core promoter. For example, a regulatory element may contain a nucleotide sequence that binds with cellular factors enabling transcription exclusively or preferentially in particular cells, tissues, or organelles. These types of regulatory elements are normally associated with genes that are expressed in a “cell-specific,” “tissue-specific,” or “organelle-specific” manner.

An “enhancer” is a type of regulatory element that can increase the efficiency of transcription, regardless of the distance or orientation of the enhancer relative to the start site of transcription.

“Heterologous DNA” refers to a DNA molecule, or a population of DNA molecules, that does not exist naturally within a given host cell. DNA molecules heterologous to a particular host cell may contain DNA derived from the host cell species (i.e., endogenous DNA) so long as that host DNA is combined with non-host DNA (i.e., exogenous DNA). For example, a DNA molecule containing a non-host DNA segment encoding a polypeptide operably linked to a host DNA segment comprising a transcription promoter is considered to be a heterologous DNA molecule. Conversely, a heterologous DNA molecule can comprise an endogenous gene operably linked with an exogenous promoter. As another illustration, a DNA molecule comprising a gene derived from a wild-type cell is considered to be heterologous DNA if that DNA molecule is introduced into a mutant cell that lacks the wild-type gene.

A “polypeptide” is a polymer of amino acid residues preferably joined exclusively by peptide bonds, whether produced naturally or synthetically. A polypeptide produced by expression of a non-host DNA molecule is a “heterologous” peptide or polypeptide. An “amino acid residue” can be a natural or non-natural amino acid residue linked peptide bonds or bonds different from peptide bonds. The amino acid residues can be in D-configuration or L-configuration.

A “homopolymer” is a polypeptide which is built up by adding several similar polypeptides to an original polypeptide thereby creating multiple copies of the same polypeptide as one larger polypeptide.

A “heteropolymer” is a polypeptide which is built up by adding several different polypeptides to an original polypeptide thereby creating multiple copies of the different polypeptide as one larger polypeptide.

A “non-bulky amino acid residue” is preferably a natural amino acid excluding amino acids having either a cyclic (aliphatic or aromatic) side chain, such as e.g. Pro, Phe, Trp, Tyr and His, or a long or branched aliphatic side chain, such as e.g. Arg, Lys, Leu, Ile, Met and Val, or more generally, a bulky amino acid has a side chain having at least 3 carbons, which are linked and form a branched or unbranched side chain. Presently preferred examples of “non-bulky amino acids” comprise Gly, Ala and Ser.

A “polypeptide according to the present invention” is any polypeptide cited in the claims of the present patent application or the patent granted on the basis of claims of this patent application.

A “polynucleotide according to the present invention” or a “nucleic acid according to the present invention” is any polynucleotide encoding a “polypeptide according to the present invention”, including any polypeptide cited in the claims of the present patent application or the patent granted on the basis of claims of this patent application.

An “integrated genetic element” is a segment of DNA that has been incorporated into a chromosome of a host cell after that element is introduced into the cell through human manipulation. Within the present invention, integrated genetic elements are most commonly derived from linearized plasmids that are introduced into the cells by electroporation or other techniques. Integrated genetic elements are passed from the original host cell to its progeny.

A “cloning vector” is a polynucleotide molecule, such as a plasmid, cosmid, or bacteriophage, that has the capability of replicating autonomously in a host cell. Cloning vectors typically contain one or a small number of restriction endonuclease recognition sites that allow insertion of a polynucleotide molecule in a determinable fashion without loss of an essential biological function of the vector, as well as nucleotide sequences encoding a marker gene that is suitable for use in the identification and selection of cells transformed with the cloning vector. Marker genes typically include genes that provide tetracycline resistance or ampicillin resistance.

An “expression vector” is a polynucleotide molecule encoding a gene that is expressed in a host cell. Typically, an expression vector comprises a transcription promoter, a gene, and a transcription terminator. Gene expression is usually placed under the control of a promoter, and such a gene is said to be “operably linked to” the promoter. Similarly, a regulatory element and a core promoter are operably linked if the regulatory element modulates the activity of the core promoter.

A “recombinant host” is a cell that contains a heterologous polynucleotide molecule, such as a cloning vector or expression vector.

“Integrative transformants” are recombinant host cells, in which heterologous DNA has become integrated into the genomic DNA of the cells.

A “fusion polypeptide” is a hybrid polypeptide expressed by a polynucleotide molecule comprising nucleotide sequences of at least two genes. For example, a fusion polypeptide can comprise at least part of a polypeptide according to the present invention fused with a polypeptide that binds an affinity matrix. Such a fusion polypeptide provides a means to isolate large quantities of a polypeptide according to the present invention using affinity chromatography.

The term “secretory signal sequence” denotes a DNA sequence that encodes a peptide (a “secretory peptide”) that, as a component of a larger polypeptide, directs the larger polypeptide through a secretory pathway of a cell in which it is synthesized. The larger polypeptide is commonly cleaved to remove the secretory peptide during transit through the secretory pathway.

An “isolated polypeptide” is a polypeptide that is essentially free from contaminating cellular components, such as carbohydrate, lipid, or other polypeptideaceous impurities associated with the polypeptide in nature. Typically, a preparation of isolated polypeptide contains the polypeptide in a highly purified form, i.e., at least about 80% pure, at least about 90% pure, at least about 95% pure, greater than 95% pure, or greater than 99% pure. One way to show that a particular polypeptide preparation contains an isolated polypeptide is by the appearance of a single band following sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the polypeptide preparation and Coomassie Brilliant Blue staining of the gel. However, the term “isolated” does not exclude the presence of the same polypeptide in alternative physical forms, such as dimers or alternatively glycosylated or derivatized forms.

The terms “amino-terminal” and “carboxyl-terminal” are used herein to denote positions within polypeptides. Where the context allows, these terms are used with reference to a particular sequence or portion of a polypeptide to denote proximity or relative position. For example, a certain sequence positioned carboxyl-terminal to a reference sequence within a polypeptide is located proximal to the carboxyl terminus of the reference sequence, but is not necessarily at the carboxyl terminus of the complete polypeptide.

The term “expression” refers to the biosynthesis of a gene product. For example, in the case of a structural gene, expression involves transcription of the structural gene into mRNA and the translation of mRNA into one or more polypeptides.

The term “splice variant” is used herein to denote alternative forms of RNA transcribed from a gene. Splice variation arises naturally through use of alternative splicing sites within a transcribed RNA molecule, or less commonly between separately transcribed RNA molecules, and may result in several mRNAs transcribed from the same gene. Splice variants may encode polypeptides having altered amino acid sequence. The term splice variant is also used herein to denote a polypeptide encoded by a splice variant of an mRNA transcribed from a gene.

The term “complement/anti-complement pair” denotes non-identical moieties that form a non-covalently associated, stable pair under appropriate conditions. For instance, biotin and avidin (or streptavidin) are prototypical members of a complement/anti-complement pair. Other exemplary complement/anti-complement pairs include receptor/ligand pairs, antibody/antigen (or hapten or epitope) pairs, sense/antisense polynucleotide pairs, and the like. Where subsequent dissociation of the complement/anti-complement pair is desirable, the complement/anti-complement pair preferably has a binding affinity of less than 10⁹ M⁻¹.

An “anti-idiotype antibody” is an antibody that binds with the variable region domain of an immunoglobulin. In the present context, an anti-idiotype antibody binds with the variable region of an anti-antibody, and thus, an anti-idiotype antibody mimics an epitope of a polypeptide according to the present invention.

An “antibody fragment” is a portion of an antibody such as F(ab′)₂, F(ab)₂, Fab′, Fab, and the like. Regardless of structure, an antibody fragment binds with the same antigen that is recognized by the intact antibody. For example, an anti-(polypeptide according to the present invention) monoclonal antibody fragment binds an epitope of a polypeptide according to the present invention.

The term “antibody fragment” also includes a synthetic or a genetically engineered polypeptide that binds to a specific antigen, such as polypeptides consisting of the light chain variable region, “Fv” fragments consisting of the variable regions of the heavy and light chains, recombinant single chain polypeptide molecules in which light and heavy variable regions are connected by a peptide linker (“scFv polypeptides”), and minimal recognition units consisting of the amino acid residues that mimic the hypervariable region.

A “chimeric antibody” is a recombinant polypeptide that contains the variable domains and complementary determining regions derived from a rodent antibody, while the remainder of the antibody molecule is derived from a human antibody.

“Humanized antibodies” are recombinant polypeptides in which murine complementarity determining regions of a monoclonal antibody have been transferred from heavy and light variable chains of the murine immunoglobulin into a human variable domain.

A “detectable label” is a molecule or atom which can be conjugated to an antibody moiety to produce a molecule useful for diagnosis. Examples of detectable labels include chelators, photoactive agents, radioisotopes, fluorescent agents, paramagnetic ions, or other marker moieties.

The term “affinity tag” is used herein to denote a polypeptide segment that can be attached to a second polypeptide to provide for purification or detection of the second polypeptide or provide sites for attachment of the second polypeptide to a substrate. In principal, any peptide or polypeptide for which an antibody or other specific binding agent is available can be used as an affinity tag. Affinity tags include a poly-histidine tract, polypeptide A (Nilsson et al., EMBO J. 4:1075 (1985); Nilsson et al., Methods Enzymol. 198:3 (1991)), glutathione S transferase (Smith and Johnson, Gene 67:31 (1988)), Glu-Glu affinity tag (Grussenmeyer et al., Proc. Natl. Acad. Sci. USA 82:7952 (1985)), substance P, FLAG peptide (Hopp et al., Biotechnology 6:1204 (1988)), streptavidin binding peptide, or other antigenic epitope or binding domain. See, in general, Ford et al., polypeptide Expression and Purification 2:95 (1991). DNAs encoding affinity tags are available from commercial suppliers (e.g., Pharmacia Biotech, Piscataway, N.J.).

A “naked antibody” is an entire antibody, as opposed to an antibody fragment, which is not conjugated with a therapeutic agent. Naked antibodies include both polyclonal and monoclonal antibodies, as well as certain recombinant antibodies, such as chimeric and humanized antibodies.

As used herein, the term “antibody component” includes both an entire antibody and an antibody fragment.

A “target polypeptide” or a “target peptide” is an amino acid sequence that comprises at least one epitope, and that is expressed on a target cell, such as a tumor cell, or a cell that carries an infectious agent antigen. T cells recognize peptide epitopes presented by a major histocompatibility complex molecule to a target polypeptide or target peptide and typically lyse the target cell or recruit other immune cells to the site of the target cell, thereby killing the target cell.

An “antigenic peptide” is a peptide, which will bind a major histocompatibility complex molecule to form an MHC-peptide complex which is recognized by a T cell, thereby inducing a cytotoxic lymphocyte response upon presentation to the T cell. Thus, antigenic peptides are capable of binding to an appropriate major histocompatibility complex molecule and inducing a cytotoxic T cells response, such as cell lysis or specific cytokine release against the target cell which binds or expresses the antigen. The antigenic peptide can be bound in the context of a class I or class II major histocompatibility complex molecule, on an antigen presenting cell or on a target cell.

In eukaryotes, RNA polymerase II catalyzes the transcription of a structural gene to produce mRNA. A polynucleotide molecule can be designed to contain an RNA polymerase II template in which the RNA transcript has a sequence that is complementary to that of a specific mRNA. The RNA transcript is termed an “anti-sense RNA” and a polynucleotide molecule that encodes the anti-sense RNA is termed an “anti-sense gene.” Anti-sense RNA molecules are capable of binding to mRNA molecules, resulting in an inhibition of mRNA translation.

An “anti-sense oligonucleotide specific for a polynucletide encoding a polypeptide according to the present invention” is an oligonucleotide having a sequence (a) capable of forming a stable triplex with a portion of a gene encoding a polypeptide according to the present invention, or (b) capable of forming a stable duplex with a portion of an mRNA transcript of such a gene.

The term “variant gene” refers to polynucleotide molecules that encode a polypeptide having an amino acid sequence that is a modification of a polypeptide according to the present invention. Such variants include naturally-occurring polymorphisms of genes according to the present invention, as well as synthetic genes that contain conservative amino acid substitutions of the amino acid sequence of a polypeptide according to the present invention. Additional variant forms of genes are polynucleotide molecules that contain insertions or deletions of the nucleotide sequences described herein. A variant gene according to the present invention can be identified by determining whether the gene hybridizes with a polynucleotide molecule having the nucleotide sequence of a polypeptide according to the present invention, or its complement, under stringent conditions.

Alternatively, variant genes can be identified by sequence comparison. Two amino acid sequences have “100% amino acid sequence identity” if the amino acid residues of the two amino acid sequences are the same when aligned for maximal correspondence. Similarly, two nucleotide sequences have “100% nucleotide sequence identity” if the nucleotide residues of the two nucleotide sequences are the same when aligned for maximal correspondence. Sequence comparisons can be performed using standard software programs such as those included in the LASERGENE bioinformatics computing suite, which is produced by DNASTAR (Madison, Wis.). Other methods for comparing two nucleotide or amino acid sequences by determining optimal alignment are well-known to those of skill in the art (see, for example, Peruski and Peruski, The Internet and the New Biology: Tools for Genomic and Molecular Research (ASM Press, Inc. 1997), Wu et al. (eds.), “Information Superhighway and Computer Databases of polynucleotides and polypeptides,” in Methods in Gene Biotechnology, pages 123 151 (CRC Press, Inc. 1997), and Bishop (ed.), Guide to Human Genome Computing, 2nd Edition (Academic Press, Inc. 1998)). Particular methods for determining sequence identity are described below.

Regardless of the particular method used to identify a variant gene, a variant gene encodes a polypeptide which can be characterized by its ability to bind specifically to an anti-(polypeptide according to the invention) antibody.

The term “allelic variant” is used herein to denote any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in phenotypic polymorphism within populations. Gene mutations can be silent (no change in the encoded polypeptide) or may encode polypeptides having altered amino acid sequence. The term allelic variant is also used herein to denote a polypeptide encoded by an allelic variant of a gene.

The term “ortholog” denotes a polypeptide or polypeptide obtained from one species that is the functional counterpart of a polypeptide or polypeptide from a different species. Sequence differences among orthologs are the result of speciation.

“Paralogs” are distinct but structurally related polypeptides made by an organism. Paralogs are believed to arise through gene duplication. For example, alpha-globin, beta-globin, and myoglobin are paralogs of each other.

Due to the imprecision of standard analytical methods, molecular weights and lengths of polymers are understood to be approximate values. When such a value is expressed as “about” X or “approximately” X, the stated value of X will be understood to be accurate to +/−20%, such as +/−10%, for example +/−5%.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides in one embodiment an isolated polypeptide comprising or consisting of a sequence of amino acid residues selected from the group consisting of SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7 and SEQ ID NO:8, wherein said polypeptide is capable of reducing or inhibiting the formation and/or growth of ice crystals, or a fragment thereof capable of reducing or inhibiting the formation and/or growth of ice crystals, or a sequence which is at least 75% identical to any of said sequences.

There is also provided an isolated polynucleotide comprising a sequence of nucleotides encoding a polypeptide according to the present invention, wherein said polynucleotide can further comprise an expression signal capable of directing the expression, in a suitable host cell, of the sequence of nucleotides encoding a polypeptide according to the present invention. There is also provided a vector comprising a polynucleotide according to the present invention capable of expressing a polypeptide according to the present invention. An isolated, recombinant cell can comprise the polynucleotide according to the present invention or the vector according to the present invention or the polypeptide according to the present invention.

There is also provided an edible product comprising the polypeptide according to the present invention, wherein the edible product can be frozen, or in the form of a frozen confectionary product, such as ice cream product, or bread.

As an example of a non-food application there is provided a solid support material comprising the polypeptide according to the present invention

The present invention also pertains to methods for making or using the polypeptides according to the present invention, including, in one embodiment, a method for producing the polypeptide according to the present invention, said method comprising the steps of

-   -   i) providing the polynucleotide according to the present         invention or the vector according to the present invention,     -   ii) providing a host cell suitable for the production of a         polypeptide according to the present invention by recombinant         expression of the polynucleotide provided in step i),     -   iii) producing the polypeptide according to the present         invention, and optionally     -   iv) purifying and/or isolating said polypeptide.

When being directed to in situ production of a polypeptide according to the present invention, thereis provided a method comprising the steps of

-   -   i) providing a fermentable starting material     -   ii) providing a microorganism capable of fermenting said         fermentable food starting material and capable of producing a         polypeptide according to the present invention under suitable         conditions when fermenting said fermentable food starting         material,     -   iii) fermenting said food starting material in the presence of         said microorganism, thereby producing a fermented, edible         product,     -   wherein said fermented, edible product comprises the polypeptide         according to the present invention

In further embodiments there is provided the following methods:

A method for reducing or inhibiting ice crystal formation in a frozen, edible product, said method comprising the steps of

-   -   i) providing a frozen edible product, or one or more ingredients         required for the production thereof, and     -   ii) contacting said product and/or said ingredients, prior to,         during, or after, the production of the product, as the case may         be, with a polypeptide according to the present invention,     -   thereby reducing or inhibiting ice crystal formation in the         frozen, edible product.

A method for reducing or inhibiting ice crystal growth in a frozen, edible product, said method comprising the steps of

-   -   i) providing a frozen edible product, or one or more ingredients         required for the production thereof, and     -   ii) contacting said product and/or said ingredients, prior to,         during, or after, the production of the product, as the case may         be, with a polypeptide according to the present invention,     -   thereby reducing or inhibiting ice crystal growth in the frozen,         edible product.

A method for structuring ice crystals in a frozen, edible product, said method comprising the steps of

-   -   i) providing a frozen edible product, or one or more ingredients         required for the production thereof, and     -   ii) contacting said product and/or said ingredients, prior to,         during, or after, the production of the product, as the case may         be, with a polypeptide according to the present invention,     -   thereby structuring ice crystals in the frozen, edible product.

A method for modulating the texture or organoleptic qualities of a frozen, edible product, said method comprising the steps of

-   -   i) providing a frozen edible product, or one or more ingredients         required for the production thereof, and     -   ii) contacting said product and/or said ingredients, prior to,         during, or after, the production of the product, as the case may         be, with a polypeptide according to the present invention,     -   thereby modulating the texture or organoleptic qualities of the         frozen, edible product.

A method for monitoring ice crystal formation during the manufacture or storage of a frozen, edible product, said method comprising the steps of

-   -   i) providing a frozen edible product, or one or more ingredients         required for the production thereof, and     -   ii) contacting said product and/or said ingredients, prior to,         during, or after, the production of the product, as the case may         be, with a polypeptide according to the present invention, and     -   iii) monitoring ice crystal formation at different time points         during the manufacture or storage of the frozen, edible product.

A method for performing an in vitro fertilisation (IVF) treatment in a female individual, said method comprising the steps of removing one or more oocyte(s) from a female individual, optionally together with a biological sample comprising follicular fluid; freezing the one or more oocyte(s), optionally together with the biological sample, in the presence of a polypeptide according to the present invention; fertilising one or more of the removed oocytes in vitro; and implanting one or more of the fertilized oocytes into the female individual.

A method for increasing the likelihood or probability of pregnancy in a female individual, said method comprising the steps of removing one or more oocyte(s) from a female individual, optionally together with a biological sample comprising follicular fluid; freezing the one or more oocyte(s), optionally together with the biological sample, in the presence of a polypeptide according to the present invention; fertilising one or more of the removed oocytes in vitro; and implanting one or more fertilized oocytes into the female individual, wherein the freezing of the one or more oocyte(s) in the presence of the polypeptide according to the present invention reduces ice crystal growth and/or formation on the oocyte(s), or in an environment, wherein the oocyte(s) are present, thereby increasing the likelihood or probability of pregnancy.

The sample can further comprise granulosa-lutein cells or follicular cells and optionally also other ovarian cells recovered from the ovarian follicles of the female individual. In one embodiment, the sample further comprises frozen cells from the environment of an oocyte.

The present invention is in a further embodiment directed to a polypeptide having an ice-binding activity and comprising one or more copies of the sequence X₁-X₂-X₃-X₄-X₅-X₆-X₇-X₈-X₉ (SEQ ID NO:90), such as, for example, 2, 3, 4, 5, 6, 7, 8, 9 or 10 individually selected copies of the general ice binding domain SEQ ID NO:90, wherein X₁ is selected from the group of amino acid residues consisting of S, A, G and D;

X₂ is selected from the group of amino acid residues consisting of A, V, I, T and S;

X₃ is selected from the group of amino acid residues consisting of non-bulky amino acid residues;

X₄ is selected from the group of amino acid residues consisting of S, I, T and V;

X₅ is selected from the group of amino acid residues consisting of S, A, I and T;

X₆ is selected from the group of amino acid residues consisting of S, T and V;

X₇ is selected from the group of amino acid residues consisting of non-bulky amino acid residues;

X₈ is selected from the group of amino acid residues consisting of S, T and V;

X₉ is selected from the group of amino acid residues consisting of S, A and G; and

wherein at least one of the residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 is T or V; and

wherein the maximum number of amino acid residues of the polypeptide is less than 1000.

The maximum number of amino acid residues of a polypeptide according to the invention is preferably less than 500, such as less than 400, for example less than 300, such as less than 250, for example less than 240, such as less than 230, for example less than 220, such as less than 210, for example less than 200, such as less than 190, for example less than 180, such as less than 150, for example less than 140, such as less than 130, for example less than 120, such as less than 110, for example less than 100, such as less than 95, for example less than 90, such as less than 85, for example less than 80, such as less than 75, for example less than 70, such as less than 65, for example less than 60, such as less than 55, for example less than 50, such as less than 45, for example less than 40, such as less than 30, for example less than 20, such as less than 15.

In functional conjunction with the above-cited limitation of the size of the polypeptide, the minimum number of amino acid residues of the polypeptide according to the invention may be 10 or more, such as 12 or more, for example 14 or more, such as 16 or more, for example 18 or more, such as 20 or more, for example 22 or more, such as 24 or more, for example 26 or more, such as 28 or more, for example 30 or more, such as 32 or more, for example 34 or more, such as 36 or more, for example 38 or more, such as 40 or more, for example 42 or more, such as 44 or more, for example 46 or more, such as 48 or more, for example 50 or more, such as 55 or more, for example 60 or more, such as 65 or more, for example 70 or more, such as 75 or more, for example 80 or more, such as 85 or more, for example 90 or more, such as 95 or more, for example 100 or more, wherein, when any maximum number and minimum number is paired, the maximum number is larger than the minimum number.

In one embodiment, the polypeptide according to the invention comprises a plurality of general ice-binding domains each comprising the sequence of SEQ ID NO:90, or a variant or derivative or modification thereof, as described herein elsewhere, and preferably having 250 amino acid residues at most.

In one embodiment the invention relates to a polypeptide sequence that comprises a second sequence, in the form of a further independently selected copy of SEQ ID NO:90, wherein the further copy of SEQ ID NO:90 does not overlap with the first copy of SEQ ID NO:90. The invention in a another embodiment relates to a polypeptide which further comprises a third copy of SEQ ID NO: 90 (i.e. the polypeptide comprises three independently seleted copies of SEQ ID NO:90), and in a still further embodiment the polypeptide further comprises a fourth copy of SEQ ID NO:90 (i.e. the polypeptide comprises three independently seleted copies of SEQ ID NO:90). The independently selected copies of SEQ ID NO:90 can be identical or different as disclosed herein elsewhere.

The copies of SEQ ID NO:90 can be present in any order relative to each other, and any two sequences can be separated by at least 2 amino acid residues, such as at least 3 amino acid residues, for example at least 4 amino acid residues, such as at least 5 amino acid residues, for example at least 6 amino acid residues, such as at least 7 amino acid residues, for example at least 8 amino acid residues, such as at least 9 amino acid residues, for example at least 10 amino acid residues, such as at least 11 amino acid residues, for example at least 12 amino acid residues, such as at least 13 amino acid residues, for example at least 14 amino acid residues, such as at least 15 amino acid residues, for example at least 16 amino acid residues, such as at least 17 amino acid residues, for example at least 18 amino acid residues, such as at least 19 amino acid residues, for example at least 20 amino acid residues, such as at least 21 amino acid residues, for example at least 22 amino acid residues, such as at least 23 amino acid residues, for example at least 24 amino acid residues, such as at least 25 amino acid residues, for example at least 26 amino acid residues, such as at least 27 amino acid residues, for example at least 28 amino acid residues, such as at least 29 amino acid residues, for example at least 30 amino acid residues.

The polypeptide according to the invention can be linked to a carrier, such as a solid support or semi-solid support. The polypeptide can be covalently or non-covalently linked to any such carrier, for example a surface of a material desirably displaying the polypeptides according to the invention.

The invention further relates to a polypeptide according to the present invention fused to an affinity tag. Examples of such affinity tags are known from the litterature and can be selected from the group comprising for example: His-tag, polypeptide A tag,

Avidin/streptavidin, polypeptide G, GluthationeS-tranferase, dihyfrofolate reductase (DHFR), Green fluorescent polypeptide (GFP), polyarginine, polycysteine, c-myc, calmodulin binding polypeptide, influenzavirus hemagglutinin; maltos binding protein (MBP) (HA).

The invention also encompasses polypeptides wherein one or more amino acid residues are modified, wherein said one or more modification(s) are preferably selected from the group consisting of in vivo or in vitro chemical derivatization, such as acetylation or carboxylation, glycosylation, such as glycosylation resulting from exposing the polypeptide to enzymes which affect glycosylation, for example mammalian glycosylating or deglycosylating enzymes, phosphorylation, such as modification of amino acid residues which results in phosphorylated amino acid residues, for example phosphotyrosine, phosphoserine and phosphothreonine.

The polypeptide according to the invention can comprise one or more amino acids independently selected from the group consisting of naturally occurring L-amino acids, naturally occurring D-amino acids as well as non-naturally occuring, synthetic amino acids.

The invention also relates to polypeptides of the invention where blocking groups are introduced in order to protect and/or stabilize the N- and/or C-termini of the polypeptide from undesirable degradation. Such blocking groups may be selected from the group comprising branched or non-branched alkyl groups and acyl groups, such as formyl and acetly groups, as well substituted froms thereof, such as the acetamidomethyl.

The invention further relates to modifications and derivatives of the polypeptide according to the invention, nucleotides encoding said polypeptides, vectors comprising said nucleotides, host cells transformed with said vectors and transgenic organisms comprising said cells.

Patent Despositions Under the Budapest Treaty

The following bacterial strains have been deposited on 4 of Jun. 2007 with the DSMZ under the provisions of the Budapest Treaty:

Escherichia coli AL03231

DSM 19401: E. coli strain JM109 containing plasmid pGEM-T-Easy-RmAFP1

Escherichia coli AL03232

DSM 19402: E. coli strain JM109 containing plasmid pGEM-T-Easy-RmAFP2

Escherichia coli AL03233

DSM 19403: E. coli strain JM109 containing plasmid pGEM-T-Easy-RmAFP3

Escherichia coli AL03234

DSM 19404: E. coli strain JM109 containing plasmid pGEM-T-Easy-RmAFP4

Escherichia coli AL03235

DSM 19405: E. coli strain JM109 containing plasmid pGEM-T-Easy-RmAFP5

Escherichia coli AL03236

DSM 19406: E. coli strain JM109 containing plasmid pGEM-T-Easy-RmAFP6

Escherichia coli AL03237

DSM 19407: E. coli strain JM109 containing plasmid pGEM-T-Easy-RmAFP7

Escherichia coli AL03238

DSM 19408: E. coli strain JM109 containing plasmid pGEM-T-Easy-RmAFP8

The following statement is made for the below cited regional areas and national states in relation to the above-cited DSMZ deposition numbers.

EPO: The applicant hereby requests that until the publication of the mention of the grant of a European Patent or for 20 years from the date of filing if the application is refused or withdrawn or deemed to be withdrawn, the biological material shall be made available as provided in Rule 28(3) EPC only by the issue of a sample to an expert nominated by the requester (Rule 28 (4) EPC).

AUSTRALIA: The applicant hereby gives notice that the furnishing of a sample of a microorganism shall only be effected priot to the grant of a patent, or to the lapsing, refusal or withdrawal of an application, to a person who is a skilled addressee without an interest in the invention (Regulation 3.25(3) of the Autstralian Patents Regulation).

CANADA: The applicant requests that, until either a Canadian patent has been issued on the basis of the present application or the application has been refused, or is abandoned and no longer subject to reinstatement, or is withdrawn, the Commissioner of Patents only authorizes the furnishing of a sample of the deposited biological material referred to in the application to an independent expert nominated by the Commissioner.

CROATIA: The applicant hereby requests that, samples shall be, upon request, made available between the publication of the application and the granting of the patent only to an independent expert.

DENMARK: The applicant hereby requests that, until the present application has been laid open to public inspection (by the Danish Patent Office), or has been finally decided upon by the Danish Patent office without having been laid open to public inspection, the furnishing of a sample of the deposited biological material referred to in the application shall only be effected to an expert in the art.

FINLAND: The applicant hereby requests that, until the present application has been laid open to public inspection (by the National Board of Patents and Regulations), or has been finally decided upon by the National Board of Patents and Registration without having been laid open to public inspection, the furnishing of a sample of the deposited biological material referred to in the application shall only be effected to an expert in the art.

GERMANY: The applicant hereby requests that, until the grant of a patent or from 20 years from the date of filing if the application is refused or withdrawn, a sample shall only be issued to an independent expert nominated by the applicant.

ICELAND: The applicant hereby requests that until a patent has been granted or a final decision taken by the Icelandic Patent Office concerning the present application, which decision has not resulted in a patent, the furnishing of a sample of the deposited biological material referred to in the application shall only be effected to an expert in the art.

NORWAY: The applicant hereby requests that until the present application has been laid open to public inspection (by the Norwegian Patent Office), or has been finally decided upon by the Norwegian Patent Office without having been laid open inspection, the furnishing of a sample of the deposited biological material referred to in the application shall only be effected to an expert in the art.

SINGAPORE: The applicant hereby requests that the furnishing of a sample of the deposited biological material referred to in the application shall only be made available to an expert.

SPAIN: The applicant hereby requests that until the publication of the mention of the grant of a Spanish patent or for 20 years from the date of filing if the present application is refused or withdrawn, the biological material shall be made available as provided in Article 45 SPL only by the issue of a sample of the deposited biological material referred to in the application to an independent expert.

SWEDEN: The applicant hereby requests that, until the present application has been laid open to public inspection (by the Swedish Patent Office), or has been finally decided upon by the Swedish Patent Office without having been laid open to public inspection, the furnishing of a sample of the deposited biological material referred to in the application shall only be effected to an expert in the art.

UNITED KINGDOM: The applicant hereby requests that the furnishing of a sample of the deposited biological material referred to in the application shall only be made available to an expert.

Determination of Sequence Homologies and Identities

In one aspect the present invention also provides isolated polypeptides that have a substantially similar sequence identity to the polypeptides according to the present invention, such as any of SEQ ID NO:1 to SEQ ID NO:8, or their orthologs.

The term “substantially similar sequence identity” is used herein to denote polypeptides having at least 70%, such as at least 72%, for example at least 74%, such as at least 76%, for example at least 78%, such as at least 80%, for example at least 82%, such as at least 84%, for example at least 86%, such as at least 88%, for example at least 90%, such as at least 91%, for example at least 92%, such as at least 93%, for example at least 94%, such as at least 95%, for example at least 96%, such as at least 97%, for example at least 98%, such as at least 99%, or greater than 99% sequence identity to any of the sequences SEQ ID NO:1 to SEQ ID NO:8, or their orthologs.

The present invention also contemplates variant polynucleotide molecules that can be identified using two criteria: a) a determination of the identity or similarity between a polypeptide having the amino acid sequence of any of the sequences SEQ ID NO:1 to SEQ ID NO:8, cf above, and b) a hybridization assay carried out under stringent conditions. For example, certain gene variants comprise polynucleotides that remain hybridized with a polynucleotide encoding a polypeptide according to the present invention, such as any of the sequences SEQ ID NO:1 to SEQ ID NO:8, or a complement of such a polynucleotide, following washing under stringent washing conditions, in which the wash stringency is equivalent to 0.5× to 2×SSC with 0.1% SDS at 55° C. to 65° C. Alternatively, variant genes can be characterized as polynucleotide molecules that remain hybridized with a polynucleotide encoding a polypeptide according to the present invention, such as any of the sequences SEQ ID NO:1 to SEQ ID NO:8, or a complement of such a polynucleotide, following washing under stringent washing conditions, in which the wash stringency is equivalent to 0.1× to 0.2×SSC with 0.1% SDS at 55° C. to 65° C.

Percent sequence identity is determined by conventional methods. See, for example, Altschul et al., Bull. Math. Bio. 48:603 (1986), and Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 (1992). Briefly, two amino acid sequences are aligned to optimize the alignment scores using a gap opening penalty of 10, a gap extension penalty of 1, and the “BLOSUM62” scoring matrix of Henikoff and Henikoff (ibid.). The percent identity is then calculated as: ([Total number of identical matches]/[length of the longer sequence plus the number of gaps introduced into the longer sequence in order to align the two sequences])×(100).

Those skilled in the art appreciate that there are many established algorithms available to align two amino acid sequences. The “FASTA” similarity search algorithm of Pearson and Lipman is a suitable polypeptide alignment method for examining the level of identity shared by an amino acid sequence disclosed herein and the amino acid sequence of a putative or variant. The FASTA algorithm is described by Pearson and Lipman, Proc. Nat'l Acad. Sci. USA 85:2444 (1988), and by Pearson, Meth. Enzymol. 183:63 (1990).

Briefly, FASTA first characterizes sequence similarity by identifying regions shared by the query sequence (e.g., any of the sequences SEQ ID NO:1 to SEQ ID NO:8) and a test sequence that have either the highest density of identities (if the ktup variable is 1) or pairs of identities (if ktup=2), without considering conservative amino acid substitutions, insertions, or deletions. The ten regions with the highest density of identities are then rescored by comparing the similarity of all paired amino acids using an amino acid substitution matrix, and the ends of the regions are “trimmed” to include only those residues that contribute to the highest score. If there are several regions with scores greater than the “cutoff” value (calculated by a predetermined formula based upon the length of the sequence and the ktup value), then the trimmed initial regions are examined to determine whether the regions can be joined to form an approximate alignment with gaps. Finally, the highest scoring regions of the two amino acid sequences are aligned using a modification of the Needleman-Wunsch-Sellers algorithm (Needleman and Wunsch, J. Mol. Biol. 48:444 (1970); Sellers, SIAM J. Appl. Math. 26:787 (1974)), which allows for amino acid insertions and deletions. Preferred parameters for FASTA analysis are: ktup=1, gap opening penalty=10, gap extension penalty=1, and substitution matrix=BLOSUM62. These parameters can be introduced into a FASTA program by modifying the scoring matrix file (“SMATRIX”), as explained in Appendix 2 of Pearson, Meth. Enzymol. 183:63 (1990).

FASTA can also be used to determine the sequence identity of polynucleotide molecules using a ratio as disclosed above. For nucleotide sequence comparisons, the ktup value can range between one to six, preferably from three to six, and most preferably, three. The other parameters can be set as: gap opening penalty=10, and gap extension penalty=1.

Substitution of Amino Acid Residues in Polypeptides According to the Present Invention

The present invention is also directed to polypeptides having one or more conservative amino acid substitution(s) and polynucleotides encoding polypeptides having one or more conservative amino acid substitution(s), as compared with the amino acid sequence of any of the sequences SEQ ID NO:1 to SEQ ID NO:8. That is, variants can be obtained that contain one or more amino acid substitutions of any of the sequences SEQ ID NO:1 to SEQ ID NO:8. Variants include sequences wherein an alkyl amino acid is substituted for an alkyl amino acid, wherein an aromatic amino acid is substituted for an aromatic amino acid, wherein a sulfur-containing amino acid is substituted for a sulfur-containing amino acid in, wherein a hydroxy-containing amino acid is substituted for a hydroxy-containing amino acid, wherein an acidic amino acid is substituted for an acidic amino acid, wherien a basic amino acid is substituted for a basic amino acid, or wherein a dibasic monocarboxylic amino acid is substituted for a dibasic monocarboxylic amino acid.

Among the common amino acids, for example, a “conservative amino acid substitution” can also be illustrated by a substitution among amino acids within each of the following groups: (1) glycine, alanine, valine, leucine, and isoleucine, (2) phenylalanine, tyrosine, and tryptophan, (3) serine and threonine, (4) aspartate and glutamate, (5) glutamine and asparagine, and (6) lysine, arginine and histidine.

The BLOSUM62 table is an amino acid substitution matrix derived from about 2,000 local multiple alignments of polypeptide sequence segments, representing highly conserved regions of more than 500 groups of related polypeptides (Henikoff and Henikoff, Proc. Nat'l Acad. Sci. USA 89:10915 (1992)). Accordingly, the BLOSUM62 substitution frequencies can be used to define conservative amino acid substitutions that may be introduced into the amino acid sequences of the present invention.

Although it is possible to design amino acid substitutions based solely upon chemical properties (as discussed above), the language “conservative amino acid substitution” preferably refers to a substitution represented by a BLOSUM62 value of greater than—1. For example, an amino acid substitution is conservative if the substitution is characterized by a BLOSUM62 value of 0, 1, 2, or 3. According to this system, preferred conservative amino acid substitutions are characterized by a BLOSUM62 value of at least 1 (e.g., 1, 2 or 3), while more preferred conservative amino acid substitutions are characterized by a BLOSUM62 value of at least 2 (e.g., 2 or 3).

Particular variants of polypeptides are characterized by having at least 70%, at least 80%, at least 85%, at least 90%, at least 95% or greater than 95% sequence identity to a corresponding amino acid sequence disclosed herein (i.e., any of the sequences SEQ ID NO:1 to SEQ ID NO:8), e.g. when the variation in amino acid sequence is due to one or more conservative amino acid substitutions.

Variants of amino acid sequences, such as “conservative amino acid” variants, can be obtained, for example, by oligonucleotide-directed mutagenesis, linker-scanning mutagenesis, mutagenesis using the polymerase chain reaction, and the like (see Ausubel (1995) at pages 8 10 to 8 22; and McPherson (ed.), Directed Mutagenesis: A Practical Approach (IRL Press 1991)).

The polypeptides according to the present invention can also comprise non-naturally occurring amino acid residues. Non-naturally occurring amino acids include e.g., without limitation, trans-3-methylproline, 2,4-methanoproline, cis-4-hydroxyproline, trans-4-hydroxyproline, N-methylglycine, allo-threonine, methylthreonine, hydroxyethylcysteine, hydroxyethylhomocysteine, nitroglutamnine, homoglutamine, pipecolic acid, thiazolidine carboxylic acid, dehydroproline, 3- and 4-methylproline, 3,3-dimethylproline, tert-leucine, norvaline, 2-azaphenylalanine, 3-azaphenylalanine, 4-azaphenylalanine, and 4-fluorophenylalanine.

Several methods are known in the art for incorporating non-naturally occurring amino acid residues into polypeptides. For example, an in vitro system can be employed wherein nonsense mutations are suppressed using chemically aminoacylated suppressor tRNAs. Methods for synthesizing amino acids and aminoacylating tRNA are known in the art. Transcription and translation of plasmids containing nonsense mutations is typically carried out in a cell-free system comprising an E. coli S30 extract and commercially available enzymes and other reagents. polypeptides are purified by chromatography. See, for example, Robertson et al., J. Am. Chem. Soc. 113:2722 (1991), Ellman et al., Methods Enzymol. 202:301 (1991), Chung et al., Science 259:806 (1993), and Chung et al., Proc. Nat'l Acad. Sci. USA 90:10145 (1993).

Multiple amino acid substitutions can be made and tested using known methods of mutagenesis and screening, such as those disclosed by Reidhaar-Olson and Sauer (Science 241:53 (1988)) or Bowie and Sauer (Proc. Nat'l Acad. Sci. USA 86:2152 (1989)). Briefly, these authors disclose methods for simultaneously randomizing two or more positions in a polypeptide, selecting for functional polypeptide, and then sequencing the mutagenized polypeptides to determine the spectrum of allowable substitutions at each position. Other methods that can be used include phage display (e.g., Lowman et al., Biochem. 30:10832 (1991), Ladner et al., U.S. Pat. No. 5,223,409, Huse, international publication No. WO 92/06204, and region-directed mutagenesis (Derbyshire et al., Gene 46:145 (1986), and Ner et al., DNA 7:127, (1988)).

Variants of the disclosed nucleotide and polypeptide sequences according to the present invention can also be generated through DNA shuffling as disclosed by Stemmer, Nature 370:389 (1994), Stemmer, Proc. Nat'l Acad. Sci. USA 91:10747 (1994), and international publication No. WO 97/20078. Briefly, variant DNA molecules are generated by in vitro homologous recombination by random fragmentation of a parent DNA followed by reassembly using PCR, resulting in randomly introduced point mutations. This technique can be modified by using a family of parent DNA molecules, such as allelic variants or DNA molecules from different species, to introduce additional variability into the process. Selection or screening for the desired activity, followed by additional iterations of mutagenesis and assay provides for rapid “evolution” of sequences by selecting for desirable mutations while simultaneously selecting against detrimental changes.

Mutagenesis methods as disclosed herein can be combined with high-throughput, automated screening methods to detect activity of cloned, mutagenized polypeptides in host cells. Mutagenized DNA molecules that encode biologically active polypeptides, or polypeptides that bind specific antibodies, can be recovered from the host cells and rapidly sequenced using modern equipment. These methods allow the rapid determination of the importance of individual amino acid residues in a polypeptide of interest, and can be applied to polypeptides of unknown structure.

Fragments of Polypeptides According to the Present Invention

The present invention also includes “functional fragments” of polypeptides and polynucleotide molecules according to the present invention encoding such functional fragments. Routine deletion analyses of polynucleotide molecules can be performed to obtain functional fragments of a polynucleotide molecule that encodes a polypeptide according to the present inventon. As an illustration, DNA molecules encoding any of the sequences SEQ ID NO:1 to SEQ ID NO:8 can be digested with Ba131 nuclease to obtain a series of nested deletions. The fragments are then inserted into expression vectors in proper reading frame, and the expressed polypeptides are isolated and tested for the ability to bind specifically to anti-antibodies. One alternative to exonuclease digestion is to use oligonucleotide-directed mutagenesis to introduce deletions or stop codons to specify production of a desired fragment. Alternatively, particular fragments of a gene according to the present inventon can be synthesized using the polymerase chain reaction.

Fragments of SEQ ID NO:1 to SEQ ID NO:8 preferably comprises one or more of the ice binding sites denoted SEQ ID NO:9 to SEQ ID NO:72, as described herein elsewhere. Chimeric polypeptides and polypeptide fragments are also provided.

The following fragment: GSYSCRAVGVDASTVTDVQGTCHAKATGPGAVASGTSVDGSTSTATATGSC originates from the full length sequences SEQ ID NO 1, SEQ ID NO 2, SEQ NO 7 and SEQID NO 8 and contains the alternative residues C in the C-terminal end and GS in the N-terminal end.

Methods for identifying functional domains are well-known to those of skill in the art. For example, studies on the truncation at either or both termini of interferons have been summarized by Horisberger and Di Marco, Pharmac. Ther. 66:507 (1995). Moreover, standard techniques for functional analysis of polypeptides are described by, for example, Treuter et al., Molec. Gen. Genet. 240:113 (1993), Content et al., “Expression and preliminary deletion analysis of the 42 kDa 2 5 A synthetase induced by human interferon,” in Biological Interferon Systems, Proceedings of ISIR-TNO Meeting on Interferon Systems, Cantell (ed.), pages 65 72 (Nijhoff 1987), Herschman, “The EGF Receptor,” in Control of Animal Cell Proliferation, Vol. 1, Boynton et al., (eds.) pages 169 199 (Academic Press 1985), Coumailleau et al., J. Biol. Chem. 270:29270 (1995); Fukunaga et al., J. Biol. Chem. 270:25291 (1995); Yamaguchi et al., Biochem. Pharmacol. 50:1295 (1995), and Meisel et al., Plant Molec. Biol. 30:1 (1996).

The present invention also contemplates functional fragments of a polypeptide according to the present inventon that have amino acid changes, compared with the amino acid sequence of any of the sequences SEQ ID NO:1 to SEQ ID NO:8. A variant polypeptide can be identified on the basis of structure by determining the level of identity with a particular amino acid sequence disclosed herein. An alternative approach to identifying a variant polypeptide on the basis of structure is to determine whether a polynucleotide molecule encoding a potential variant polypeptide can hybridize to a polynucleotide molecule having the nucleotide sequence of any of the sequences SEQ ID NO:1 to SEQ ID NO:8, as discussed above.

The present invention also provides polypeptide fragments or peptides comprising an epitope-bearing portion of a polypeptide according to the present inventon as described herein. Such fragments or peptides may comprise an “immunogenic epitope,” which is a part of a polypeptide that elicits an antibody response when the entire polypeptide is used as an immunogen. Immunogenic epitope-bearing peptides can be identified using standard methods (see, for example, Geysen et al., Proc. Nat'l Acad. Sci. USA 81:3998 (1983)).

In contrast, polypeptide fragments or peptides may comprise an “antigenic epitope,” which is a region of a polypeptide molecule to which an antibody can specifically bind. Certain epitopes consist of a linear or contiguous stretch of amino acids, and the antigenicity of such an epitope is not disrupted by denaturing agents. It is known in the art that relatively short synthetic peptides that can mimic epitopes of a polypeptide can be used to stimulate the production of antibodies against the polypeptide (see, for example, Sutcliffe et al., Science 219:660 (1983)). Accordingly, antigenic epitope-bearing peptides and polypeptides of the present invention are useful to raise antibodies that bind with the polypeptides described herein.

Antigenic epitope-bearing peptides and polypeptides can contain at least four to ten amino acids, such as at least ten to fifteen amino acids, for example about 15 to about 30 amino acids of any of the sequences SEQ ID NO:1 to SEQ ID NO:8. Such epitope-bearing peptides and polypeptides can be produced by fragmenting a polypeptide according to the present inventon, or by chemical peptide synthesis, as described herein. Moreover, epitopes can be selected by phage display of random peptide libraries (see, for example, Lane and Stephen, Curr. Opin. Immunol. 5:268 (1993), and Cortese et al., Curr. Opin. Biotechnol. 7:616 (1996)). Standard methods for identifying epitopes and producing antibodies from small peptides that comprise an epitope are described, for example, by Mole, “Epitope Mapping,” in Methods in Molecular Biology, Vol. 10, Manson (ed.), pages 105 116 (The Humana Press, Inc. 1992), Price, “Production and Characterization of Synthetic Peptide-Derived Antibodies,” in Monoclonal Antibodies: Production, Engineering, and Clinical Application, Ritter and Ladyman (eds.), pages 60 84 (Cambridge University Press 1995), and Coligan et al. (eds.), Current Protocols in Immunology, pages 9.3.1 9.3.5 and pages 9.4.1 9.4.11 (John Wiley & Sons 1997).

Regardless of the particular nucleotide sequence of a variant gene according to the present inventon, the gene encodes a polypeptide that may be characterized by its ability to bind specifically to an antibody capable of specifically binding to any of the sequences SEQ ID NO:1 to SEQ ID NO:8.

Fusion Polypeptides Comprising Anti-Freeze Polypeptides or Ice Binding Sites or Ice Binding Domains According to the Invention

The present invention also includes anti-freeze fusion polypeptides. Anti-freeze fusion polypeptides of the present invention may be targeted to a particular cellular compartment or to the extracellular space, to a particular cell or to particular cell types. By attachment of polypeptide segments which specify or determine targeting to cellular compartments, the anti-freeze segments may be targeted to a particular cellular organelle. Not only will the peptide be directed to the organelle, but the anti-freeze function may remain functional even when surrounded by other polypeptide segments. By fusion to antibodies or other molecules having cell specificity in binding, the resistance to cellular damage upon freezing can be conferred to those cell types. This technique will also find use in organs. Examples of polypeptides to which the polypeptide according to the present invention can be bound are listed below:

Fussion to (protein) Advantages of fusion Protein A Detectability, secretion from GRAM-positive bacteria, purification, readily cleaved to yield free peptide Beta-Galactosidase Measurement by enzyme assay, detectability on Western Blots Beta-lactamase Detectability on Western Blots, secretion to periplasm in GRAM-negative bacteria Chlortamphenicol Detectability on Western blots, measurement acetyltransferase by enzyme assay in plant extracts Pathogenesis-related Secretion from dicotyledonous plants polypeptide prib Alpha-amylase Secretion from monocotyledonous plants Phytohemagglutinin Vacuole targeting in plants RuBPCASE small Chloroplast targeting in plants subunit Phaseolin Accumulation in seeds Alcohol dehydrogenase Expression in yeast Alpha mating factor Secretion from yeast Luciferase Detectability by light emmision

Fusion polypeptides comprising polypeptides according to the present invention can thus be used to express a polypeptide according to the present invention in a recombinant host, and to isolate expressed polypeptides. One type of fusion polypeptide comprises a peptide that guides a polypeptide according to the present invention from a recombinant host cell. To direct a polypeptide according to the present invention into the secretory pathway of a eukaryotic host cell, a secretory signal sequence (also known as a signal peptide, a leader sequence, prepro sequence or pre sequence) is provided in a suitable expression vector. While the secretory signal sequence may be derived from a polypeptide according to the present invention, a suitable signal sequence may also be derived from another secreted polypeptide or synthesized de novo. The secretory signal sequence is operably linked to a gene encoding sequence according to the present invention such that the two sequences are joined in the correct reading frame and positioned to direct the newly synthesized polypeptide into the secretory pathway of the host cell. Secretory signal sequences are commonly positioned 5′ to the nucleotide sequence encoding the polypeptide of interest, although certain secretory signal sequences may be positioned elsewhere in the nucleotide sequence of interest (see, e.g., Welch et al., U.S. Pat. No. 5,037,743; Holland et al., U.S. Pat. No. 5,143,830).

Although the secretory signal sequence of a gene according to the present invention, or another polypeptide produced by mammalian cells (e.g., tissue-type plasminogen activator signal sequence, as described, for example, in U.S. Pat. No. 5,641,655) is useful for expression of a gene according to the present invention in recombinant mammalian hosts, a yeast signal sequence is preferred for expression in yeast cells. Examples of suitable yeast signal sequences are those derived from yeast mating phermone alpha-factor (encoded by the MF-alpha1 gene), invertase (encoded by the SUC2 gene), or acid phosphatase (encoded by the PHO5 gene). See, for example, Romanos et al., “Expression of Cloned Genes in Yeast,” in DNA Cloning 2: A Practical Approach, 2.sup.nd Edition, Glover and Hames (eds.), pages 123 167 (Oxford University Press 1995).

In bacterial cells, it is often desirable to express a heterologous polypeptide as a fusion polypeptide to decrease toxicity, increase stability, and to enhance recovery of the expressed polypeptide. For example, a gene according to the present invention can be expressed as a fusion polypeptide comprising a glutathione S-transferase polypeptide. Glutathione S-transferease fusion polypeptides are typically soluble, and easily purifiable from E. coli lysates on immobilized glutathione columns. In similar approaches, a fusion polypeptide according to the present invention comprising a maltose binding polypeptide polypeptide can be isolated with an amylose resin column, while a fusion polypeptide comprising the C-terminal end of a truncated polypeptide A gene can be purified using IgG-Sepharose. Established techniques for expressing a heterologous polypeptide as a fusion polypeptide in a bacterial cell are described, for example, by Williams et al., “Expression of Foreign polypeptides in E. coli Using Plasmid Vectors and Purification of Specific Polyclonal Antibodies,” in DNA Cloning 2: A Practical Approach, 2.sup.nd Edition, Glover and Hames (Eds.), pages 15 58 (Oxford University Press 1995). In addition, commercially available expression systems are available. For example, the PINPOINT Xa polypeptide purification system (Promega Corporation; Madison, Wis.) provides a method for isolating a fusion polypeptide comprising a polypeptide that becomes biotinylated during expression with a resin that comprises avidin.

Peptide tags that are useful for isolating heterologous polypeptides expressed by either prokaryotic or eukaryotic cells include polyHistidine tags (which have an affinity for nickel-chelating resin), c-myc tags, calmodulin binding polypeptide (isolated with calmodulin affinity chromatography), substance P, the RYIRS tag (which binds with anti-RYIRS antibodies), the Glu-Glu tag, and the FLAG tag (which binds with anti-FLAG antibodies). See, for example, Luo et al., Arch. Biochem. Biophys. 329:215 (1996), Morganti et al., Biotechnol. Appl. Biochem. 23:67 (1996), and Zheng et al., Gene 186:55 (1997). polynucleotide molecules encoding such peptide tags are available, for example, from Sigma-Aldrich Corporation (St. Louis, Mo.).

Another form of fusion polypeptide comprises a polypeptide according to the present invention and an immunoglobulin heavy chain constant region, typically an F_(c) fragment, which contains two constant region domains and a hinge region but lacks the variable region. As an illustration, Chang et al., U.S. Pat. No. 5,723,125, describe a fusion polypeptide comprising a human interferon and a human immunoglobulin Fc fragment. The C-terminal of the interferon is linked to the N-terminal of the Fc fragment by a peptide linker moiety. An example of a peptide linker is a peptide comprising primarily a T cell inert sequence, which is immunologically inert. An exemplary peptide linker has the amino acid sequence: GGSGG SGGGG SGGGG S (SEQ ID NO:91). In this fusion polypeptide, a preferred F_(c) moiety is a human gamma4 chain, which is stable in solution and has little or no complement activating activity. Accordingly, the present invention contemplates a fusion polypeptide that comprises a polypeptide according to the present invention, or a fragment thereof, and a human F_(c) fragment, wherein the C-terminus of the polypeptide according to the present invention, or a fragment thereof, is attached to the N-terminus of the F_(c) fragment via a peptide linker.

In another variation, a fusion polypeptide comprising a polypeptide according to the present invention further comprises an IgG sequence. The polypeptide moiety according to the present invention is covalently joined to the amino terminal end of the IgG sequence, and a signal peptide that is covalently joined to the amino terminal of the polypeptide moiety according to the present invention, wherein the IgG sequence comprises or consists of the following elements in the following order: a hinge region, a CH₂ domain, and a CH₃ domain. Accordingly, the IgG sequence lacks a CH₁ domain. The polypeptide moiety according to the present invention displays an ice-binding activity. The above, general approaches for producing fusion polypeptides that comprise both antibody and nonantibody portions has been described by LaRochelle et al., EP 742830 (WO 95/21258).

Fusion polypeptides can be prepared by methods known to those skilled in the art by preparing each component of the fusion polypeptide and chemically conjugating them. Alternatively, a polynucleotide encoding both components of the fusion polypeptide in the proper reading frame can be generated using known techniques and expressed by the methods described herein. General methods for enzymatic and chemical cleavage of fusion polypeptides are described, for example, by Ausubel (1995) at pages 16 19 to 16 25.

General Methods for the Production of Polypeptides and Fragments thereof According to the Present Invention

Synthesis of anti-freeze polypeptides according to the present invention may be pursued in two forms, either biological or synthetic. The biological method is by expression of polypeptide coding sequence or gene; the synthetic method is by chemical synthesis of a polypeptide.

A preferred synthetic method utilizes solid phase peptide synthesis, such as that developed by Merrifield (J. Am. Chem. Soc., (1963) 85:2149-2156). This method will be particularly useful in testing particular compositions or formulations for anti-freeze activity.

For large scale production, the biological expression would typically be preferred. The encoding polynucleotide or gene can be a natural gene with recombinant modifications or a totally synthetic sequence that will be expressed in an appropriate expression system. The methods utilized for insertion of a natural sequence segment into an appropriate vector are well known to persons of ordinary skill in the art, see Maniatis or Wu, et al. (1987) Methods in Enzymology, Vol. 153, Academic Press, New York, N.Y.

Synthetic sequences can be synthesized by the phosphoramidite chemistry to make particular sections of the sequence (Beaucage and Carruthers, (1981) Tet. Letters, 22:1859-1862). Overlapping segments can be synthesized and then ligated together to produce a larger gene.

Finally, by selecting particular sequences for the anti-freeze segments, restriction enzyme cutting sites may be introduced which will provide convenient segments which may be easily linked together or inserted to generate tandem repeats, as will be obvious to one of ordinary skill in the art.

Purification of the anti-freeze polypeptides will be by methods known to a person of ordinary skill in the art of polypeptide purification. Standard purification techniques may be from either cell lysates or culture medium if the polypeptides are secreted. Typical methods are column chromatography, ammonium sulfate salt precipitations, antibody affinity column chromatography and others. With naturally occurring polypeptides (e.g., produced in fish), a preferred method of purification is as described by DeVries et al. (1977) Biochem Biophys. Acta 495:388-392.

Preferably, the anti-freeze polypeptides will be purified to substantial homogeneity, usually at least about 70% to 80% pure, preferably about 90-95% pure, most preferably 99% or more pure. Typically, the polypeptides will be substantially free of contaminating, naturally associated fish compounds.

It is clear from the above that the polypeptides of the present invention, including full-length polypeptides, functional fragments, and fusion polypeptides, can advantageously be produced in recombinant host cells following conventional techniques.

To express a gene according to the present invention, a polynucleotide molecule encoding the polypeptide must be operably linked to regulatory sequences that control transcriptional expression in an expression vector and then, introduced into a host cell. In addition to transcriptional regulatory sequences, such as promoters and enhancers, expression vectors can include translational regulatory sequences and a marker gene, which is suitable for selection of cells that carry the expression vector.

Expression vectors that are suitable for production of a foreign polypeptide in eukaryotic cells typically contain (1) prokaryotic DNA elements coding for a bacterial replication origin and an antibiotic resistance marker to provide for the growth and selection of the expression vector in a bacterial host; (2) eukaryotic DNA elements that control initiation of transcription, such as a promoter; and (3) DNA elements that control the processing of transcripts, such as a transcription termination/polyadenylation sequence.

As discussed above, expression vectors can also include nucleotide sequences encoding a secretory sequence that directs the heterologous polypeptide into the secretory pathway of a host cell. For example, an expression vector may comprise a gene according to the present invention and a secretory sequence derived from said gene or another secreted gene.

Examples of vectors commonly used with bacteria include the pET series (Novagen), pGEX series (Ge Healthcare), pBAD-series (Invitrogen). Examples of vectors in yeasts are the pPic series for Pichia (Invitrogen), the pKlac system from Kluyveromyces lactis (New England biolabs), S. cereviseae vectors (Patel, O., Fearnley, R., and Macreadie, I. 3002. Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tags. Biotechnol Lett. 2003 25(4):331-334) and the pYes system for S. cereviseae (Invitrogen).

Examples of vectors for use in funghi are the pBAR series (described in Pall, M. L. and J. Brunelli. 1993. A series of six compact fungal transformation vectors containing polylinkers with unique restrictions sites. Fungal Genetics Newsletter 40: 59-61). The pIEx plasmid based system (Merck) or the baculovirus based system (Merck) are two examples of systems useful for insect cells. Similar products are available from other companies.

Examples of vectors for use in insect cells include the tetracycline regulated systems pTet and pTre, the adenovirus-based system Adeno-X, the retrovirus-based system Rethro-X (all Clontech) and the pcDNA vectors (Invitrogen). Again, many more examples exist and are on the market.

Polypeptides according to the present invention may be expressed in mammalian cells. Examples of suitable mammalian host cells include African green monkey kidney cells (Vero; ATCC CRL 1587), human embryonic kidney cells (293-HEK; ATCC CRL 1573), baby hamster kidney cells (BHK-21, BHK-570; ATCC CRL 8544, ATCC CRL 10314), canine kidney cells (MDCK; ATCC CCL 34), Chinese hamster ovary cells (CHO-K1; ATCC CCL61; CHO DG44 [Chasin et al., Som. Cell. Molec. Genet. 12:555 1986]), rat pituitary cells (GH1; ATCC CCL82), HeLa S3 cells (ATCC CCL2.2), rat hepatoma cells (H-4-II-E; ATCC CRL 1548) SV40-transformed monkey kidney cells (COS-1; ATCC CRL 1650) and murine embryonic cells (NIH-3T3; ATCC CRL 1658).

For a mammalian host, the transcriptional and translational regulatory signals may be derived from viral sources, such as adenovirus, bovine papilloma virus, simian virus, or the like, in which the regulatory signals are associated with a particular gene which has a high level of expression. Suitable transcriptional and translational regulatory sequences also can be obtained from mammalian genes, such as actin, collagen, myosin, and metallothionein genes.

Transcriptional regulatory sequences include a promoter region sufficient to direct the initiation of RNA synthesis. Suitable eukaryotic promoters include the promoter of the mouse metallothionein I gene (Hamer et al., J. Molec. Appl. Genet. 1:273 (1982)), the TK promoter of Herpes virus (McKnight, Cell 31:355 (1982)), the SV40 early promoter (Benoist et al., Nature 290:304 (1981)), the Rous sarcoma virus promoter (Gorman et al., Proc. Nat'l Acad. Sci. USA 79:6777 (1982)), the cytomegalovirus promoter (Foecking et al., Gene 45:101 (1980)), and the mouse mammary tumor virus promoter (see, generally, Etcheverry, “Expression of Engineered polypeptides in Mammalian Cell Culture,” in polypeptide Engineering: Principles and Practice, Cleland et al. (eds.), pages 163 181 (John Wiley & Sons, Inc. 1996)).

Alternatively, a prokaryotic promoter, such as the bacteriophage T3 RNA polymerase promoter, can be used to control gene expression in mammalian cells if the prokaryotic promoter is regulated by a eukaryotic promoter (Zhou et al., Mol. Cell. Biol. 10:4529 (1990), and Kaufman et al., Nucl. Acids Res. 19:4485 (1991)).

An expression vector can be introduced into host cells using a variety of standard techniques including calcium phosphate transfection, liposome-mediated transfection, microprojectile-mediated delivery, electroporation, and the like. The transfected cells can be selected and propagated to provide recombinant host cells that comprise the expression vector stably integrated in the host cell genome.

Techniques for introducing vectors into eukaryotic cells and techniques for selecting such stable transformants using a dominant selectable marker are described, for example, by Ausubel (1995) and by Murray (ed.), Gene Transfer and Expression Protocols (Humana Press 1991). A gene according to the present invention may thus be expressed in higher eukaryots, such as avian, fungal, insect, yeast, and plant cells.

For example, one suitable selectable marker is a gene that provides resistance to the antibiotic neomycin. In this case, selection is carried out in the presence of a neomycin-type drug, such as G-418 or the like. Selection systems can also be used to increase the expression level of the gene of interest, a process referred to as “amplification.” Amplification is carried out by culturing transfectants in the presence of a low level of the selective agent and then increasing the amount of selective agent to select for cells that produce high levels of the products of the introduced genes.

A suitable amplifiable selectable marker is dihydrofolate reductase, which confers resistance to methotrexate. Other drug resistance genes (e.g., hygromycin resistance, multi-drug resistance, puromycin acetyltransferase) can also be used. Alternatively, markers that introduce an altered phenotype, such as green fluorescent polypeptide, or cell surface polypeptides such as CD4, CD8, Class I MHC, placental alkaline phosphatase may be used to sort transfected cells from untransfected cells by such means as FACS sorting or magnetic bead separation technology.

Polypeptides according to the present invention can also be produced by cultured mammalian cells using a viral delivery system. Exemplary viruses for this purpose include adenovirus, herpesvirus, vaccinia virus and adeno-associated virus (AAV). Adenovirus, a double-stranded DNA virus, is currently the best studied gene transfer vector for delivery of heterologous polynucleotide (for a review, see Becker et al., Meth. Cell Biol. 43:161 (1994), and Douglas and Curiel, Science & Medicine 4:44 (1997)). Advantages of the adenovirus system include the accommodation of relatively large DNA inserts, the ability to grow to high-titer, the ability to infect a broad range of mammalian cell types, and flexibility that allows use with a large number of available vectors containing different promoters.

By deleting portions of the adenovirus genome, larger inserts (up to 7 kb) of heterologous DNA can be accommodated. These inserts can be incorporated into the viral DNA by direct ligation or by homologous recombination with a co-transfected plasmid. An option is to delete the essential E1 gene from the viral vector, which results in the inability to replicate unless the E1 gene is provided by the host cell. Adenovirus vector-infected human 293 cells (ATCC Nos. CRL-1573, 45504, 45505), for example, can be grown as adherent cells or in suspension culture at relatively high cell density to produce significant amounts of polypeptide (see Garnier et al., Cytotechnol. 15:145 (1994)).

Methods for generating transgenic organisms are known in the art, cf Table 3:

Bacteria, Yeasts and Fungi

-   -   The transgenic organisms are obtained simply by introducing the         relevant expression plasmids. Methods for this are listed herein         elsewhere     -   Insects: Li W, Jin L, An L. 2003. Construction of targeting         vector and expression of green fluorescent polypeptide in the         silkworm, Antheraea pernyi. DNA Cell Biol. 22:441-6. Yamao M,         Katayama N, Nakazawa H, Yamakawa M, Hayashi Y, Hara S, Kamei K,         Mori H.1999.         -   Gene targeting in the silkworm by use of a baculovirus.Genes             Dev. 13:511-6. Allen M L, Scholl P J. 2005. Quality of             transgenic laboratory strains of Cochliomyia hominivorax             (Diptera: Calliphoridae).J Econ Entomol. 98:2301-6.     -   Plants See p. 47 of the patent (Horsch et al., Science 227:1229         (1985), Klein et al., Biotechnology 10:268 (1992), and Miki et         al., “Procedures for Introducing Foreign DNA into Plants,” in         Methods in Plant Molecular Biology and Biotechnology, Glick et         al. (eds.), pages 67 88 (CRC Press, 1993).     -   Fish Rembold M, Lahiri K, Foulkes N S, Wittbrodt J. 2006.         Transgenesis in fish: efficient selection of transgenic fish by         co-injection with a fluorescent reporter construct. Nature         Protocols 1:1133-9         -   Rahman M A, Mak R, Ayad H, Smith A, Maclean N. 1998.             Expression of a novel piscine growth hormone gene results in             growth enhancement in transgenic tilapia (Oreochromis             niloticus). Transgenic Res. 7:357-69. Uzbekova S, Chyb J,             Ferriere F, Bailhache T, Prunet P, Alestrom P,             Breton B. 2000. Transgenic rainbow trout expressed             sGnRH-antisense RNA under the control of sGnRH promoter of             Atlantic salmon. J Mol Endocrinol. 25:337-50.     -   Animals Nagashima H, Fujimura T, Takahagi Y, Kurome M, Wako N,         Ochiai T, Esaki R, Kano K, Saito S, Okabe M, Murakami H. 2003.         Development of efficient strategies for the production of         genetically modified pigs. Theriogenology. 59:95-106         -   Lai L, Prather R S. 2003. Creating genetically modified pigs             by using nuclear transfer. Reprod Biol Endocrinol. 1:82         -   Hofmann A, Kessler B, Ewerling S, Weppert M, Vogg B, Ludwig             H, Stojkovic M, Boelhauve M, Brem G, Wolf E,             Pfeifer A. 2003. Efficient transgenesis in farm animals by             lentiviral vectors. EMBO Rep. 4:1054-60.             DNA microinjection     -   (Gordon, J. W. and Ruddle, F. H. 1981. Integration and stable         germ line transformation of genes injected into mouse pronuclei.         Science 214:1244-1246),         Embryonic stem cell-mediated gene transfer Retrovirus-mediated         gene transfer     -   Transgenesis by means of blastocyst-derived embryonic stem cell         line. Proc. Natl. Acad. Sci. 83:9065-9069)     -   Jaenisch, R. 1976. Germ line integration and Mendelian         transmission of the exogenous Moloney leukemia virus. Proc.         Natl. Acad. Sci. 73:1260-1264)

The baculovirus system provides an efficient means to introduce cloned genes according to the present invention into insect cells. Suitable expression vectors are based upon the Autographa californica multiple nuclear polyhedrosis virus (AcMNPV), and contain well-known promoters such as Drosophila heat shock polypeptide (hsp) 70 promoter, Autographa californica nuclear polyhedrosis virus immediate-early gene promoter (ie-1) and the delayed early 39K promoter, baculovirus p10 promoter, and the Drosophila metallothionein promoter.

A second method of making recombinant baculovirus utilizes a transposon-based system described by Luckow (Luckow, et al., J. Virol. 67:4566 (1993)). This system, which utilizes transfer vectors, is sold in the BAC-to-BAC kit (Life Technologies, Rockville, Md.). This system utilizes a transfer vector, PFASTBAC (Life Technologies) containing a Tn7 transposon to move the DNA encoding the polypeptide according to the present invention into a baculovirus genome maintained in E. coli as a large plasmid called a “bacmid.” See, Hill-Perkins and Possee, J. Gen. Virol. 71:971 (1990), Bonning, et al., J. Gen. Virol. 75:1551 (1994), and Chazenbalk, and Rapoport, J. Biol. Chem. 270:1543 (1995).

In addition, transfer vectors can include an in-frame fusion with DNA encoding an epitope tag at the C- or N-terminus of the expressed polypeptide according to the present invention, for example, a Glu-Glu epitope tag (Grussenmeyer et al., Proc. Nat'l Acad. Sci. 82:7952 (1985)). Using a technique known in the art, a transfer vector containing a gene according to the present invention is transformed into E. coli, and screened for bacmids, which contain an interrupted lacZ gene indicative of recombinant baculovirus. The bacmid DNA containing the recombinant baculovirus genome is then isolated using common techniques.

The illustrative PFASTBAC vector can be modified to a considerable degree. For example, the polyhedrin promoter can be removed and substituted with the baculovirus basic polypeptide promoter (also known as Pcor, p6.9 or MP promoter) which is expressed earlier in the baculovirus infection, and has been shown to be advantageous for expressing secreted polypeptides (see, for example, Hill-Perkins and Possee, J. Gen. Virol. 71:971 (1990), Bonning, et al., J. Gen. Virol. 75:1551 (1994), and Chazenbalk and Rapoport, J. Biol. Chem. 270:1543 (1995). In such transfer vector constructs, a short or long version of the basic polypeptide promoter can be used. Moreover, transfer vectors can be constructed which replace the native secretory signal sequences of polypeptides according to the present invention with secretory signal sequences derived from insect polypeptides. For example, a secretory signal sequence from Ecdysteroid Glucosyltransferase (EGT), honey bee Melittin (Invitrogen Corporation; Carlsbad, Calif.), or baculovirus gp67 (PharMingen: San Diego, Calif.) can be used in constructs to replace native secretory signal sequences.

The recombinant virus or bacmid is used to transfect host cells. Suitable insect host cells include cell lines derived from IPLB-Sf-21, a Spodoptera frugiperda pupal ovarian cell line, such as Sf9 (ATCC CRL 1711), Sf21AE, and Sf21 (Invitrogen Corporation; San Diego, Calif.), as well as Drosophila Schneider-2 cells, and the HIGH FIVEO cell line (Invitrogen) derived from Trichoplusia ni (U.S. Pat. No. 5,300,435). Commercially available serum-free media can be used to grow and to maintain the cells. Suitable media are Sf900 II™ (Life Technologies) or ESF 921™ (Expression Systems) for Sf9 cells; and Ex-cellO405™ (JRH Biosciences, Lenexa, Kans.) or Express FiveO™ (Life Technologies) for T. ni cells. When recombinant virus is used, the cells are typically grown up from an inoculation density of approximately 2 to 5×10⁵ cells to a density of 1 to 2×10⁶ cells at which time a recombinant viral stock is added at a multiplicity of infection (MOI) of 0.1 to 10, more typically near 3.

Established techniques for producing recombinant polypeptides in baculovirus systems are provided by Bailey et al., “Manipulation of Baculovirus Vectors,” in Methods in Molecular Biology, Volume 7: Gene Transfer and Expression Protocols, Murray (ed.), pages 147 168 (The Humana Press, Inc. 1991), by Patel et al., “The baculovirus expression system,” in DNA Cloning 2: Expression Systems, 2nd Edition, Glover et al. (eds.), pages 205 244 (Oxford University Press 1995), by Ausubel (1995) at pages 16 37 to 16 57, by Richardson (ed.), Baculovirus Expression Protocols (The Humana Press, Inc. 1995), and by Lucknow, “Insect Cell Expression Technology,” in polypeptide Engineering: Principles and Practice, Cleland et al. (eds.), pages 183 218 (John Wiley & Sons, Inc. 1996).

Fungal cells, including yeast cells, can also be used to express the genes described herein. Yeast species of particular interest in this regard include Saccharomyces cerevisiae, Pichia pastoris, and Pichia methanolica. Suitable promoters for expression in yeast include promoters from GAL1 (galactose), PGK (phosphoglycerate kinase), ADH (alcohol dehydrogenase), AOX1 (alcohol oxidase), HIS4 (histidinol dehydrogenase), and the like. Many yeast cloning vectors have been designed and are readily available. These vectors include Ylp-based vectors, such as YIp5, YRp vectors, such as YRp17, YEp vectors such as YEp13 and YCp vectors, such as YCp19. Methods for transforming S. cerevisiae cells with exogenous DNA and producing recombinant polypeptides therefrom are disclosed by, for example, Kawasaki, U.S. Pat. No. 4,599,311, Kawasaki et al., U.S. Pat. No. 4,931,373, Brake, U.S. Pat. No. 4,870,008, Welch et al., U.S. Pat. No. 5,037,743, and Murray et al., U.S. Pat. No. 4,845,075. Transformed cells are selected by phenotype determined by the selectable marker, commonly drug resistance or the ability to grow in the absence of a particular nutrient (e.g., leucine). A suitable vector system for use in Saccharomyces cerevisiae is the POT1 vector system disclosed by Kawasaki et al. (U.S. Pat. No. 4,931,373), which allows transformed cells to be selected by growth in glucose-containing media.

Additional suitable promoters and terminators for use in yeast include those from glycolytic enzyme genes (see, e.g., Kawasaki, U.S. Pat. No. 4,599,311, Kingsman et al., U.S. Pat. No. 4,615,974, and Bitter, U.S. Pat. No. 4,977,092) and alcohol dehydrogenase genes. See also U.S. Pat. Nos. 4,990,446, 5,063,154, 5,139,936, and 4,661,454. Other examples of commonly used and/or commercially available vectors suitable for use in yeast are the pPic series (Invitrogen), the pKlac system from Kluyveromyces lactis (New England Biolabs) and S. cerevisae vectors (Patel et al., Biotechnology letters 2003 vol 25(4):331-334) as well as the pYes system for S. cerevisae (Invitrogen). In fungi, the pBAR series is useful (Pall et al., 1993 vol. 40:59-61, Functional Genetics Newsletter).

Transformation systems for other yeasts, including Hansenula polymorpha, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces fragilis, Ustilago maydis, Pichia pastoris, Pichia methanolica, Pichia guillermondii and Candida maltosa are known in the art. See, for example, Gleeson et al., J. Gen. Microbiol. 132:3459 (1986), and Cregg, U.S. Pat. No. 4,882,279. Aspergillus cells may be utilized according to the methods of McKnight et al., U.S. Pat. No. 4,935,349. Methods for transforming Acremonium chrysogenum are disclosed by Sumino et al., U.S. Pat. No. 5,162,228. Methods for transforming Neurospora are disclosed by Lambowitz, U.S. Pat. No. 4,486,533.

For example, the use of Pichia methanolica as host for the production of recombinant polypeptides is disclosed by Raymond, U.S. Pat. No. 5,716,808, Raymond, U.S. Pat. No. 5,736,383, Raymond et al., Yeast 14:11 23 (1998), and in international publication Nos. WO 97/17450, WO 97/17451, WO 98/02536, and WO 98/02565. DNA molecules for use in transforming P. methanolica will commonly be prepared as double-stranded, circular plasmids, which can be linearized prior to transformation. For polypeptide production in P. methanolica, the promoter and terminator in the plasmid can be that of a P. methanolica gene, such as a P. methanolica alcohol utilization gene (AUG1 or AUG2). Other useful promoters include those of the dihydroxyacetone synthase (DHAS), formate dehydrogenase (FMD), and catalase (CAT) genes. To facilitate integration of the DNA into the host chromosome, it is preferred to have the entire expression segment of the plasmid flanked at both ends by host DNA sequences. A suitable selectable marker for use in Pichia methanolica is a P. methanolica ADE2 gene, which encodes phosphoribosyl-5-aminoimidazole carboxylase (AIRC; EC 4.1.1.21), and which allows ade2 host cells to grow in the absence of adenine. For large-scale, industrial processes where it is desirable to minimize the use of methanol, it is possible to use host cells in which both methanol utilization genes (AUG1 and AUG2) are deleted. For production of secreted polypeptides, host cells can be used that are deficient in vacuolar pro tease genes (PEP4 and PRB1). Electroporation is used to facilitate the introduction of a plasmid containing DNA encoding a polypeptide of interest into P. methanolica cells. P. methanolica cells can be transformed by electroporation using an exponentially decaying, pulsed electric field having a field strength of from 2.5 to 4.5 kV/cm, preferably about 3.75 kV/cm, and a time constant (t) of from 1 to 40 milliseconds, most preferably about 20 milliseconds.

Expression vectors can also be introduced into plant protoplasts, intact plant tissues, or isolated plant cells. Methods for introducing expression vectors into plant tissue include the direct infection or co-cultivation of plant tissue with Agrobacterium tumefaciens, microprojectile-mediated delivery, DNA injection, electroporation, and the like. See, for example, Horsch et al., Science 227:1229 (1985), Klein et al., Biotechnology 10:268 (1992), and Miki et al., “Procedures for Introducing Foreign DNA into Plants,” in Methods in Plant Molecular Biology and Biotechnology, Glick et al. (eds.), pages 67 88 (CRC Press, 1993).

Alternatively, genes according to the present invention can be expressed in prokaryotic host cells. Suitable promoters that can be used to express polypeptides according to the present invention in a prokaryotic host are well-known to those of skill in the art and include promoters capable of recognizing the T4, T3, Sp6 and T7 polymerases, the P_(R) and P_(L) promoters of bacteriophage lambda, the trp, recA, heat shock, lacUV5, tac, lpp-lacSpr, phoA, and lacZ promoters of E. coli, promoters of B. subtilis, the promoters of the bacteriophages of Bacillus, Streptomyces promoters, the int promoter of bacteriophage lambda, the bla promoter of pBR322, and the CAT promoter of the chloramphenicol acetyl transferase gene. Prokaryotic promoters have been reviewed by Glick, J. Ind. Microbiol. 1:277 (1987), Watson et al., Molecular Biology of the Gene, 4th Ed. (Benjamin Cummins 1987), and by Ausubel et al. (1995).

Suitable prokaryotic hosts include E. coli and Bacillus subtilus. Suitable strains of E. coli include BL21(DE3), BL21(DE3)pLysS, BL21(DE3)pLysE, DH1, DH4I, DHS, DH5I, DH5IF, DH5IMCR, DH10B, DH10B/p3, DH11S, C600, HB101, JM101, JM105, JM109, JM110, K38, RR1, Y1088, Y1089, CSH18, ER1451, and ER1647 (see, for example, Brown (ed.), Molecular Biology Labfax (Academic Press 1991)). Suitable strains of Bacillus subtilus include BR151, YB886, MI119, MI120, and B170 (see, for example, Hardy, “Bacillus Cloning Methods,” in DNA Cloning: A Practical Approach, Glover (ed.) (IRL Press 1985)).

When expressing a polypeptide according to the present invention in bacteria such as E. coli, the polypeptide may be retained in the cytoplasm, typically as insoluble granules, or may be directed to the periplasmic space by a bacterial secretion sequence. In the former case, the cells are lysed, and the granules are recovered and denatured using, for example, guanidine isothiocyanate or urea. The denatured polypeptide can then be refolded and dimerized by diluting the denaturant, such as by dialysis against a solution of urea and a combination of reduced and oxidized glutathione, followed by dialysis against a buffered saline solution. In the latter case, the polypeptide can be recovered from the periplasmic space in a soluble and functional form by disrupting the cells (by, for example, sonication or osmotic shock) to release the contents of the periplasmic space and recovering the polypeptide, thereby obviating the need for denaturation and refolding.

Methods for expressing polypeptides in prokaryotic hosts are well-known to those of skill in the art (see, for example, Williams et al., “Expression of foreign polypeptides in E. coli using plasmid vectors and purification of specific polyclonal antibodies,” in DNA Cloning 2: Expression Systems, 2nd Edition, Glover et al. (eds.), page 15 (Oxford University Press 1995), Ward et al., “Genetic Manipulation and Expression of Antibodies,” in Monoclonal Antibodies: Principles and Applications, page 137 (Wiley-Liss, Inc. 1995), and Georgiou, “Expression of polypeptides in Bacteria,” in polypeptide Engineering: Principles and Practice, Cleland et al. (eds.), page 101 (John Wiley & Sons, Inc. 1996)).

Standard methods for introducing expression vectors into bacterial, yeast, insect, and plant cells are provided, for example, by Ausubel (1995).

General methods for expressing and recovering foreign polypeptide produced by a mammalian cell system are provided by, for example, Etcheverry, “Expression of Engineered polypeptides in Mammalian Cell Culture,” in polypeptide Engineering: Principles and Practice, Cleland et al. (eds.), pages 163 (Wiley-Liss, Inc. 1996). Standard techniques for recovering polypeptide produced by a bacterial system is provided by, for example, Grisshammer et al., “Purification of over-produced polypeptides from E. coli cells,” in DNA Cloning 2: Expression Systems, 2nd Edition, Glover et al. (eds.), pages 59 92 (Oxford University Press 1995). Established methods for isolating recombinant polypeptides from a baculovirus system are described by Richardson (ed.), Baculovirus Expression Protocols (The Humana Press, Inc. 1995).

As an alternative, polypeptides of the present invention can be synthesized by exclusive solid phase synthesis, partial solid phase methods, fragment condensation or classical solution synthesis. These synthesis methods are well-known to those of skill in the art (see, for example, Merrifield, J. Am. Chem. Soc. 85:2149 (1963), Stewart et al., “Solid Phase Peptide Synthesis” (2nd Edition), (Pierce Chemical Co. 1984), Bayer and Rapp, Chem. Pept. Prot. 3:3 (1986), Atherton et al., Solid Phase Peptide Synthesis: A Practical Approach (IRL Press 1989), Fields and Colowick, “Solid-Phase Peptide Synthesis,” Methods in Enzymology Volume 289 (Academic Press 1997), and Lloyd-Williams et al., Chemical Approaches to the Synthesis of Peptides and polypeptides (CRC Press, Inc. 1997)). Variations in total chemical synthesis strategies, such as “native chemical ligation” and “expressed polypeptide ligation” are also standard (see, for example, Dawson et al., Science 266:776 (1994), Hackeng et al., Proc. Nat'l Acad. Sci. USA 94:7845 (1997), Dawson, Methods Enzymol. 287: 34 (1997), Muir et al, Proc. Nat'l Acad. Sci. USA 95:6705 (1998), and Severinov and Muir, J. Biol. Chem. 273:16205 (1998)).

The present invention contemplates compositions comprising a peptide or polypeptide described herein. Such compositions can further comprise a carrier. The carrier can be a conventional organic or inorganic carrier. Examples of carriers include water, buffer solution, alcohol, propylene glycol, macrogol, sesame oil, corn oil, and the like.

Isolation of Polypeptides According to the Present Invention

The polypeptides of the present invention can be purified to at least about 80% purity, to at least about 90% purity, to at least about 95% purity, or even greater than 95% purity with respect to contaminating macromolecules, particularly other polypeptides and polynucleotides, and free of infectious and pyrogenic agents. The polypeptides of the present invention can also be purified to a pharmaceutically pure state, which is greater than 99.9% pure. In certain preparations, a purified polypeptide is substantially free of other polypeptides, particularly other polypeptides of animal origin.

Fractionation and/or conventional purification methods can be used to obtain preparations of polypeptides according to the present invention purified from natural sources, and recombinant polypeptides according to the present invention and fusion polypeptides according to the present invention purified from recombinant host cells. In general, ammonium sulfate precipitation and acid or chaotrope extraction may be used for fractionation of samples. Exemplary purification steps may include hydroxyapatite, size exclusion, FPLC and reverse-phase high performance liquid chromatography. Suitable chromatographic media include derivatized dextrans, agarose, cellulose, polyacrylamide, specialty silicas, and the like. PEI, DEAE, QAE and Q derivatives are preferred. Exemplary chromatographic media include those media derivatized with phenyl, butyl, or octyl groups, such as Phenyl-Sepharose FF (Pharmacia), Toyopearl butyl 650 (Toso Haas, Montgomeryville, Pa.), Octyl-Sepharose (Pharmacia) and the like; or polyacrylic resins, such as Amberchrom CG 71 (Toso Haas) and the like. Suitable solid supports include glass beads, silica-based resins, cellulosic resins, agarose beads, cross-linked agarose beads, polystyrene beads, cross-linked polyacrylamide resins and the like that are insoluble under the conditions in which they are to be used. These supports may be modified with reactive groups that allow attachment of polypeptides by amino groups, carboxyl groups, sulfhydryl groups, hydroxyl groups and/or carbohydrate moieties.

Examples of coupling chemistries include cyanogen bromide activation, N-hydroxysuccinimide activation, epoxide activation, sulfhydryl activation, hydrazide activation, and carboxyl and amino derivatives for carbodiimide coupling chemistries. These and other solid media are well known and widely used in the art, and are available from commercial suppliers. Selection of a particular method for polypeptide isolation and purification is a matter of routine design and is determined in part by the properties of the chosen support. See, for example, Affinity Chromatography: Principles & Methods (Pharmacia LKB Biotechnology 1988), and Doonan, polypeptide Purification Protocols (The Humana Press 1996).

Additional variations in the isolation and purification of polypeptides according to the present invention can be devised by those of skill in the art. For example, specific antibodies recognising polypeptides according to the present invention and fragments thereof, obtained as described below, can be used to isolate large quantities of polypeptide by immunoaffinity purification.

The polypeptides of the present invention can also be isolated by exploitation of particular properties. For example, immobilized metal ion adsorption (IMAC) chromatography can be used to purify histidine-rich polypeptides, including those comprising polyhistidine tags. Briefly, a gel is first charged with divalent metal ions to form a chelate (Sulkowski, Trends in Biochem. 3:1 (1985)). Histidine-rich polypeptides will be adsorbed to this matrix with differing affinities, depending upon the metal ion used, and will be eluted by competitive elution, lowering the pH, or use of strong chelating agents. Other methods of purification include purification of glycosylated polypeptides by lectin affinity chromatography and ion exchange chromatography (M. Deutscher, (ed.), Meth. Enzymol. 182:529 (1990)). Within additional embodiments of the invention, a fusion of the polypeptide of interest and an affinity tag (e.g., maltose-binding polypeptide, an immunoglobulin domain) may be constructed to facilitate purification.

Polypeptides and fragments thereof according to the present invention may also be prepared through chemical synthesis, as described above. Polypeptides according to the present invention may be monomers or multimers; glycosylated or non-glycosylated; pegylated or non-pegylated; and may or may not include an initial methionine amino acid residue.

Production of Antibodies Specific for Polypeptides According to the Present Invention

Antibodies to an ice-binding polypeptide according to the present invention, or a fragment thereof, can be obtained, for example, by using as an antigen the product produced from an expression vector comprising a gene according to the present invention in a suitable host organism, or by using a polypeptide according to the present invention isolated from a natural source or synthesised using any conventional solid phase synthesis strategy. Particularly useful antibodies “bind specifically” with a polypeptide according to the present invention. Antibodies are considered to be specifically binding if the antibodies exhibit at least one of the following two properties: (1) antibodies bind to a polypeptide according to the present invention with a threshold level of binding activity, and (2) antibodies do not significantly cross-react with polypeptides which are related to a polypeptide according to the present invention as defined herein below.

With regard to the first characteristic, antibodies specifically bind if they bind to a polypeptide, peptide or epitope with a binding affinity (K_(a)) of 10⁶ M⁻¹ or greater, preferably 10⁷ M⁻¹ or greater, more preferably 10⁸ M⁻¹ or greater, and most preferably 10⁹ M⁻¹ or greater. The binding affinity of an antibody can be readily determined by one of ordinary skill in the art, for example, by Scatchard analysis (Scatchard, Ann. NY Acad. Sci. 51:660 (1949)). With regard to the second characteristic, antibodies do not significantly cross-react with related polypeptide molecules, for example, if they detect polypeptides according to the present invention, but do not detect known polypeptides applied in similar or identical amounts in a standard Western blot analysis.

Antibodies can be produced using antigenic epitope-bearing peptides or polypeptides according to the present invention. Antigenic epitope-bearing peptides and polypeptides of the present invention preferably contain a sequence of at least four, or between 15 to about 30 amino acids contained within any of SEQ ID NOs:5 to 12. However, peptides or polypeptides comprising a larger portion of an amino acid sequence of the invention, containing from 30 to 50 amino acids, or any length up to and including the entire amino acid sequence of a polypeptide according to the invention, also are useful for inducing antibodies that bind with polypeptides according to the present invention. It is desirable that the amino acid sequence of the epitope-bearing peptide is selected to provide substantial solubility in aqueous solvents (i.e., the sequence includes relatively hydrophilic residues, while hydrophobic residues are preferably avoided). Moreover, amino acid sequences containing proline residues may be also be desirable for antibody production.

As an illustration, potential antigenic sites in polypeptides according to the present invention can be identified using the Jameson-Wolf method, Jameson and Wolf, CABIOS 4:181, (1988), as implemented by the PROTEAN program (version 3.14) of LASERGENE (DNASTAR; Madison, Wis.). Default parameters were used in this analysis.

The Jameson-Wolf method predicts potential antigenic determinants by combining six major subroutines for polypeptide structural prediction. Briefly, the Hopp-Woods method, Hopp et al., Proc. Nat'l Acad. Sci. USA 78:3824 (1981), was first used to identify amino acid sequences representing areas of greatest local hydrophilicity (parameter: seven residues averaged). In the second step, Emini's method, Emini et al., J. Virology 55:836 (1985), was used to calculate surface probabilities (parameter: surface decision threshold (0.6)=1). Third, the Karplus-Schultz method, Karplus and Schultz, Naturwissenschaften 72:212 (1985), was used to predict backbone chain flexibility (parameter: flexibility threshold (0.2)=1). In the fourth and fifth steps of the analysis, secondary structure predictions were applied to the data using the methods of Chou-Fasman, Chou, “Prediction of polypeptide Structural Classes from Amino Acid Composition,” in Prediction of polypeptide Structure and the Principles of polypeptide Conformation, Fasman (ed.), pages 549 586 (Plenum Press 1990), and Garnier-Robson, Garnier et al., J. Mol. Biol. 120:97 (1978) (Chou-Fasman parameters: conformation table=64 polypeptides; .alpha. region threshold=103; .beta. region threshold=105; Garnier-Robson parameters: .alpha. and .beta. decision constants=0). In the sixth subroutine, flexibility parameters and hydropathy/solvent accessibility factors were combined to determine a surface contour value, designated as the “antigenic index.” Finally, a peak broadening function was applied to the antigenic index, which broadens major surface peaks by adding 20, 40, 60, or 80% of the respective peak value to account for additional free energy derived from the mobility of surface regions relative to interior regions. This calculation was not applied, however, to any major peak that resides in a helical region, since helical regions tend to be less flexible.

Polyclonal antibodies to recombinant polypeptide or to isolated from natural sources can be prepared using methods well-known to those of skill in the art. See, for example, Green et al., “Production of Polyclonal Antisera,” in Immunochemical Protocols (Manson, ed.), pages 1 to 5 (Humana Press 1992), and Williams et al., “Expression of foreign polypeptides in E. coli using plasmid vectors and purification of specific polyclonal antibodies,” in DNA Cloning 2: Expression Systems, 2nd Edition, Glover et al. (eds.), page 15 (Oxford University Press 1995). The immunogenicity of a polypeptide can be increased through the use of an adjuvant, such as alum (aluminum hydroxide) or Freund's complete or incomplete adjuvant. Polypeptides useful for immunization also include fusion polypeptides, such as fusions of or a portion thereof with an immunoglobulin polypeptide or with maltose binding polypeptide. The polypeptide immunogen may be a full-length molecule or a portion thereof. If the polypeptide portion is “hapten-like,” such portion may be advantageously joined or linked to a macromolecular carrier (such as keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA) or tetanus toxoid) for immunization.

Although polyclonal antibodies are typically raised in animals such as horses, cows, dogs, chicken, rats, mice, rabbits, guinea pigs, goats, or sheep, an antibody specific for a polypeptides according to the present invention may also be derived from a subhuman primate antibody. General techniques for raising diagnostically and therapeutically useful antibodies in baboons may be found, for example, in Goldenberg et al., international patent publication No. WO 91/11465, and in Losman et al., Int. J. Cancer 46:310 (1990).

Alternatively, monoclonal antibodies specific for a polypeptides according to the present invention can be generated. Rodent monoclonal antibodies to specific antigens may be obtained by methods known to those skilled in the art (see, for example, Kohler et al., Nature 256:495 (1975), Coligan et al. (eds.), Current Protocols in Immunology, Vol. 1, pages 2.5.1 2.6.7 (John Wiley & Sons 1991) [“Coligan”], Picksley et al., “Production of monoclonal antibodies against polypeptides expressed in E. coli,” in DNA Cloning 2: Expression Systems, 2nd Edition, Glover et al. (eds.), page 93 (Oxford University Press 1995)).

Briefly, monoclonal antibodies can be obtained by injecting mice with a composition comprising a gene product, verifying the presence of antibody production by removing a serum sample, removing the spleen to obtain B-lymphocytes, fusing the B-lymphocytes with myeloma cells to produce hybridomas, cloning the hybridomas, selecting positive clones which produce antibodies to the antigen, culturing the clones that produce antibodies to the antigen, and isolating the antibodies from the hybridoma cultures.

In addition, an antibody specific for polypeptides according to the present invention of the present invention may be derived from a human monoclonal antibody. Human monoclonal antibodies are obtained from transgenic mice that have been engineered to produce specific human antibodies in response to antigenic challenge. In this technique, elements of the human heavy and light chain locus are introduced into strains of mice derived from embryonic stem cell lines that contain targeted disruptions of the endogenous heavy chain and light chain loci. The transgenic mice can synthesize human antibodies specific for human antigens, and the mice can be used to produce human antibody-secreting hybridomas. Methods for obtaining human antibodies from transgenic mice are described, for example, by Green et al., Nature Genet. 7:13 (1994), Lonberg et al., Nature 368:856 (1994), and Taylor et al., Int. Immun. 6:579 (1994).

Monoclonal antibodies can be isolated and purified from hybridoma cultures by a variety of well-established techniques. Such isolation techniques include affinity chromatography with polypeptide-A Sepharose, size-exclusion chromatography, and ion-exchange chromatography (see, for example, Coligan at pages 2.7.1 2.7.12 and pages 2.9.1 2.9.3; Baines et al., “Purification of Immunoglobulin G (IgG),” in Methods in Molecular Biology, Vol. 10, pages 79 104 (The Humana Press, Inc. 1992)).

For particular uses, it may be desirable to prepare fragments of antibodies specific for polypeptides according to the present invention. Such antibody fragments can be obtained, for example, by proteolytic hydrolysis of the antibody. Antibody fragments can be obtained by pepsin or papain digestion of whole antibodies by conventional methods. As an illustration, antibody fragments can be produced by enzymatic cleavage of antibodies with pepsin to provide a 5S fragment denoted F(ab′)₂. This fragment can be further cleaved using a thiol reducing agent to produce 3.5S Fab' monovalent fragments. Optionally, the cleavage reaction can be performed using a blocking group for the sulfhydryl groups that result from cleavage of disulfide linkages. As an alternative, an enzymatic cleavage using pepsin produces two monovalent Fab fragments and an F_(c) fragment directly. These methods are described, for example, by Goldenberg, U.S. Pat. No. 4,331,647, Nisonoff et al., Arch Biochem. Biophys. 89:230 (1960), Porter, Biochem. J. 73:119 (1959), Edelman et al. and Coligan, both in Methods in Enzymology Vol. 1, (Academic Press 1967).

Other methods of cleaving antibodies, such as separation of heavy chains to form monovalent light-heavy chain fragments, further cleavage of fragments, or other enzymatic, chemical or genetic techniques may also be used, so long as the fragments bind to the antigen that is recognized by the intact antibody.

For example, Fv fragments comprise an association of V_(H) and V_(L) chains. This association can be noncovalent, as described by Inbar et al., Proc. Nat'l Acad. Sci. USA 69:2659 (1972). Alternatively, the variable chains can be linked by an intermolecular disulfide bond or cross-linked by chemicals such as glutaraldehyde (see, for example, Sandhu, Crit. Rev. Biotech. 12:437 (1992)).

The Fv fragments may comprise V_(H) and V_(L) chains, which are connected by a peptide linker. These single-chain antigen binding polypeptides (scFv) are prepared by constructing a structural gene comprising DNA sequences encoding the V_(H) and V_(L) domains which are connected by an oligonucleotide. The structural gene is inserted into an expression vector, which is subsequently introduced into a host cell, such as E. coli. The recombinant host cells synthesize a single polypeptide chain with a linker peptide bridging the two V domains. Methods for producing scFvs are described, for example, by Whitlow et al., Methods: A Companion to Methods in Enzymology 2:97 (1991) (also see, Bird et al., Science 242:423 (1988), Ladner et al., U.S. Pat. No. 4,946,778, Pack et al., Bio/Technology 11:1271 (1993), and Sandhu, supra).

As an illustration, a scFV can be obtained by exposing lymphocytes to polypeptide in vitro, and selecting antibody display libraries in phage or similar vectors (for instance, through use of immobilized or labeled polypeptide or peptide). Genes encoding polypeptides having potential polypeptide binding domains can be obtained by screening random peptide libraries displayed on phage (phage display) or on bacteria, such as E. coli. Nucleotide sequences encoding the polypeptides can be obtained in a number of ways, such as through random mutagenesis and random polynucleotide synthesis. These random peptide display libraries can be used to screen for peptides, which interact with a known target which can be a polypeptide or polypeptide, such as a ligand or receptor, a biological or synthetic macromolecule, or organic or inorganic substances. Techniques for creating and screening such random peptide display libraries are known in the art (Ladner et al., U.S. Pat. No. 5,223,409, Ladner et al., U.S. Pat. No. 4,946,778, Ladner et al., U.S. Pat. No. 5,403,484, Ladner et al., U.S. Pat. No. 5,571,698, and Kay et al., Phage Display of Peptides and polypeptides (Academic Press, Inc. 1996)) and random peptide display libraries and kits for screening such libraries are available commercially, for instance from CLONTECH Laboratories, Inc. (Palo Alto, Calif.), Invitrogen Inc. (San Diego, Calif.), New England Biolabs, Inc. (Beverly, Mass.), and Pharmacia LKB Biotechnology Inc. (Piscataway, N.J.). Random peptide display libraries can be screened using the sequences disclosed herein to identify polypeptides which bind to .

Another form of an antibody fragment is a peptide coding for a single complementarity-determining region (CDR). CDR peptides (“minimal recognition units”) can be obtained by constructing genes encoding the CDR of an antibody of interest. Such genes are prepared, for example, by using the polymerase chain reaction to synthesize the variable region from RNA of antibody-producing cells (see, for example, Larrick et al., Methods: A Companion to Methods in Enzymology 2:106 (1991), Courtenay-Luck, “Genetic Manipulation of Monoclonal Antibodies,” in Monoclonal Antibodies: Production, Engineering and Clinical Application, Ritter et al. (eds.), page 166 (Cambridge University Press 1995), and Ward et al., “Genetic Manipulation and Expression of Antibodies,” in Monoclonal Antibodies: Principles and Applications, Birch et al., (eds.), page 137 (Wiley-Liss, Inc. 1995)).

Alternatively, an antibody specific for a polypeptide according to the present invention may be derived from a “humanized” monoclonal antibody. Humanized monoclonal antibodies are produced by transferring mouse complementary determining regions from heavy and light variable chains of the mouse immunoglobulin into a human variable domain. Typical residues of human antibodies are then substituted in the framework regions of the murine counterparts. The use of antibody components derived from humanized monoclonal antibodies obviates potential problems associated with the immunogenicity of murine constant regions. General techniques for cloning murine immunoglobulin variable domains are described, for example, by Orlandi et al., Proc. Nat'l Acad. Sci. USA 86:3833 (1989). Techniques for producing humanized monoclonal antibodies are described, for example, by Jones et al., Nature 321:522 (1986), Carter et al., Proc. Nat'l Acad. Sci. USA 89:4285 (1992), Sandhu, Crit. Rev. Biotech. 12:437 (1992), Singer et al., J. Immun. 150:2844 (1993), Sudhir (ed.), Antibody Engineering Protocols (Humana Press, Inc. 1995), Kelley, “Engineering Therapeutic Antibodies,” in polypeptide Engineering: Principles and Practice, Cleland et al. (eds.), pages 399 434 (John Wiley & Sons, Inc. 1996), and by Queen et al., U.S. Pat. No. 5,693,762 (1997).

Polyclonal anti-idiotype antibodies can be prepared by immunizing animals with antibodies or antibody fragments specific for a polypeptide according to the present invention, using standard techniques. See, for example, Green et al., “Production of Polyclonal Antisera,” in Methods In Molecular Biology: Immunochemical Protocols, Manson (ed.), pages 1 12 (Humana Press 1992). Also, see Coligan at pages 241 to 247. Alternatively, monoclonal anti-idiotype antibodies can be prepared using antibodies or antibody fragments specific for a polypeptide according to the present invention as immunogens with the techniques, described above. As another alternative, humanized anti-idiotype antibodies or subhuman primate anti-idiotype antibodies can be prepared using the above-described techniques. Methods for producing anti-idiotype antibodies are described, for example, by Irie, U.S. Pat. No. 5,208,146, Greene, et. al., U.S. Pat. No. 5,637,677, and Varthakavi and Minocha, J. Gen. Virol. 77:1875 (1996).

Host Cells and Uses Related Thereto

The host cells which may comprise a polypeptide according to the present invention can be examplified e.g. by animal cells, mammalian host cells, insect cells, fish cells, fungal cells, yeast cells, bacterial cells and plant cells.

The natural or synthetic nucleic fragments coding for an anti-freeze polypeptide according to the invention will be incorporated in polynucleotide constructs capable of introduction to and/or expression in the ultimate target expressing cell. Usually, the polynucleotide constructs will be suitable for replication in a unicellular or multicellular host, such as yeast or bacteria, but may also be intended for introduction and integration within the genome of cultured mammalian or other eukaryotic cell lines, in particular, plants, polynucleotide constructs prepared for introduction into bacteria or yeast will include a replication system recognized by the host, the polynucleotide fragment encoding the desired anti-freeze polypeptide product, transcriptional and translational initiation regulatory sequences joined to the 5′-end of the anti-freeze polypeptide encoding polynucleotide sequence, and transcriptional and translational termination regulatory sequences joined to the 3′-end of the sequence. The transcriptional regulatory sequences will include a heterologous promoter which is recognized by the host. Conveniently, available expression vectors which include the replication system and transcriptional and translational regulatory sequences together with an insertion site for the anti-freeze polypeptide encoding sequence may be employed.

The gene will include any polynucleotide segment which contains a coding sequence for anti-freeze polypeptide. Normally, the gene will include the coding sequence plus the upstream and downstream associated sequences, particularly any enhancer, promoter, ribosome binding site or transcription initiation markers. Downstream segments may also be important for message polyadenylation and processing, and thus are also contemplated in the usual instance.

The introduction of genes into cells or groups of cells for expression is another method for generally introducing the polypeptides into the sample of interest. The end product of the transformation is also included as the product of this invention, and the term “transformed cell” will include the actual cell transformed, and all progeny of that cell. In the typical case, the final organism will contain cells, each of which will contain the gene. Standard transformation procedures exist for bacteria (Maniatis), fungi (Sherman et al. (1986) in Laboratory Course Manual for Methods in Yeast Genetics CSH), plants (van den Elzen et al. (1985) Plant Mol. Biol., 5:149-154) and animals (Hanahan, (1988) Ann. Rev. Genetics, 22:479-519).

Yeast and Fungus Host Cells

Non-limiting examples of yeast host cells suitable for use in accordance with the present invention include yeasts from the Family of Saccharomycetaceae, including members of the genera Saccharomyces and Candida. Preferred examples include, but are not limited to, Saccharomyces fragilis, Saccharomyces cervisae, Saccharomyces lactis, Candida pseudotropicalis.

Bacteria

Bacterial cells are useful as host cells according to the present invention for the production of the polypeptides according to the present invention.

Bacteria, e.g. Lactobacillus as well as many yeasts and molds, have been used for thousands of years in the preparation of fermented foods, such as e.g. cheese, pickles, soy sauce, sauerkraut, vinegar, wine and yoghurt.

Anti-freeze polypeptides according to the present invention are useful in maintaining the viability of the microorganisms used to prepare such foods, as well as in the preparation of prebiotic and probiotic edible compositions, including animal feed compositions and foods for human consumption.

Examples of preferred bacteria relevant to the present invention and suitable as host cells in accordance with the present invention are, for example, Escherichia coli,

Streptococcus cremoris, Streptococcus lactis, Streptococcus thermophilus, Leuconostoc citrovorum, Leuconostoc mesenteroides, Lactobacillus acidophilus, Lactobacillus lactis, Lactobacillus bulgaricus, Bifidobacterium bifidum, Bifidobacterium breve, Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus. casei, and Bifidobacterium longum.

Bacteria can also be used as substitutes for pesticides in a biological pest control programme. The invention in one embodiment is particularly well suited for such applications and provide recombinant microrganisms harbouring polynucleotides according to the invention and producing polypeptides according to the invention capable of being used as environmentally friendly, biological pesticides. One example is Bacillus thuringiensis, a Gram-positive, soil dwelling bacterium.

Further non-limiting examples of bacterial host cells suitable for use in accordance with the present invention include Gram-positive bacteria and Gram-negative bacteria. Preferred bacterial cells can also be selected from the groups consisting of Gram-positive cocci, Gram-positive bacilli, Gram-negative cocci and Gram-negative bacilli. Examples of bacterial host cells suitable for use in accordance with the present invention include, but is not limited to, bacteria selected from the following genera: Acaricomes, Acetitomaculum, Acetivibrio, Acetobacter, Acetobacterium, Acetobacteroides, Acetohalobium, Acetomicrobium, Acetomonas, Acetonema, Achromobacter, Acidaminobacter, Acidaminococcus, Acidicaldus, Acidimicrobium, Acidiphilium, Acidithiobacillus, Acidobacterium, Acidocaldus, Acidocella, Acidomonas, Acidovorax, Acinetobacter, Acrocarpospora, Actinacidiphilus, Actinoacidiphilus, Actinoalloteichus, Actinobacillus, Actinobaculum, Actinobifida, Actinobispora, Actinocatenispora, Actinocorallia, Actinokineospora, Actinomadura, Actinomyces, Actinoplanes, Actinopolyspora, Actinopycnidium, Actinospica, Actinosporangium, Actinostreptospora, Actinosynnema, Actinotalea, Actinotelluria, Adhaeribacter, Aequorivita, Aerobacter, Aerococcus, Aerococcus-like Organism, Aeromicrobium, Aeromonas, Aestuariibacter, Afipia, Agarbacterium, Aggregatibacter, Agitococcus, Agreia, Agrobacterium, Agrococcus, Agromonas, Agromyces, Ahrensia, Albidovulum, Alcaligenes, Alcanivorax, Algibacter, Algicola, Algoriphagus, Alicycliphilus, Alicyclobacillus, Alishewanella, Alistipes, Alkalibacillus, Alkalibacter, Alkalibacterium, Alkalilimnicola, Alkalispirillum, Alkanindiges, Allisonella, Allobaculum, Allochromatium, Allofustis, Alteromonas, Alysiella, Aminobacter, Aminobacterium, Aminomonas, Ammonifex, Ammoniphilus, Amoebobacter, Amorphosporangium, Amphibacillus, Ampullariella, Amycolata, Amycolatopsis, Anaeroarcus, Anaerobacter, Anaerobaculum, Anaerobiospirillum, Anaerobranca, Anaerocellum, Anaerococcus, Anaerofilum, Anaerofustis, Anaerolinea, Anaeromusa, Anaerophaga, Anaeroplasma, Anaerosinus, Anaerostipes, Anaerotruncus, Anaerovibrio, Anaerovirgula, Anaerovorax, Ancalomicrobium, Ancylobacter, Aneurinibacillus, Angiococcus, Angulomicrobium, Anoxybacillus, Antarctobacter, Aquabacter, Aquabacterium, Aquamicrobium, Aquaspirillum, Aquicella, Aquifex, Aquiflexum, Aquimarina, Aquimonas, Arcanobacterium, Archangium, Arcicella, Arcobacter, Arenibacter, Arhodomonas, Arizona, Arsenicicoccus, Arsenophonus, Arthrobacter, Asanoa, Asiosporangium, Asticcacaulis, Astrosporangium, Atopobium, Atopococcus, Atopostipes, Aurantimonas, Aureobacterium, Avibacterium, Axonoporis, Azoarcus, Azohydromonas, Azomonas, Azorhizobium, Azorhizophilus, Azospira, Azospirillum, Azotobacter Bacillus, Bacteriovorax, Bacterium, Bacteroides, Balnearium, Balneatrix, Barnesiella, Bartonella, Bdellovibrio, Beggiatoa, Beijerinckia, Belliella, Belnapia, Beneckea, Bergeriella, Betabacterium, Beutenbergia, Bifidobacterium, Bilophila, Blastobacter, Blastochloris, Blastococcus, Blastomonas, Blastopirellula, Bogoriella, Bordetella, Borrelia, Bosea, Brachybacterium, Brachymonas, Brachyspira, Brackiella, Bradyrhizobium, Branhamella, Brenneria, Brevibacillus, Brevibacterium, Brevigemma, Brevundimonas, Brochothrix, Brucella, Bryantella, Budvicia, Bulleidia, Burkholderia, Buttiauxella, Butyribacterium, Butyrivibrio, Byssovorax, Caenibacterium, Caldanaerobacter, Caldicellulosiruptor, Caldilinea, Caldithrix, Caldocellum, Caloramator, Caloranaerobacter, Caminibacillus, Caminibacter, Caminicella, Campylobacter, Capnocytophaga, Carbophilus, Carboxydocella, Carboxydothermus, Cardiobacterium, Carnobacterium, Caryophanon, Caseobacter, Castellaniella, Cat Scratch Disease Bacillus, Catellatospora, Catellibacterium, Catellicoccus, Catenibacterium, Catenococcus, Catenulispora, Catenuloplanes, Catenulospora, Caulobacter, Cdc Enteric Group 36/37, Cdc Group Vd, Cedecea, Cellulomonas, Cellulophaga, Cellulosimicrobium, Cellvibrio, Centipeda, Cerasibacillus, Chainia, Chelatobacter, Chelatococcus, Chitinibacter, Chitinimonas, Chitinophaga, Chlorobaculum, Chlorobium, Chloroflexus, Chondrococcus, Chondromyces, Chromatium, Chromobacterium, Chromohalobacter, Chryseobacterium, Chryseomonas, Chrysiogenes, Citreicella, Citricoccus, Citrobacter, Clavibacter, Clavisporangium, Clo Group Type 2, Clostridium, Cobetia, Cohnella, Collimonas, Collinsella, Colwellia, Comamonas, Conchiformibius, Conexibacter, Coprothermobacter, Corallococcus, Coriobacterium, Corynebacterium, Couchioplanes, Crossiella, Cryobacterium, Cryptanaerobacter, Cryptobacterium, Cryptosporangium, Cupriavidus, Curtobacterium, Curvibacter, Cyclobacterium, Cystobacter, Cytophaga, Dactylosporangium, Dechloromonas, Dechlorosoma, Deefgea, Deferribacter, Defluvibacter, Dehalobacter, Dehalospirillum, Deinococcus, Deleya, Delftia, Demetria, Dendrosporobacter, Denitrovibrio, Dermabacter, Dermacoccus, Dermatophilus, Derxia, Desemzia, Desulfacinum, Desulfarculus, Desulfatibacillum, Desulfitobacterium, Desulfoarculus, Desulfobacca, Desulfobacter, Desulfobacterium, Desulfobacula, Desulfobulbus, Desulfocapsa, Desulfocella, Desulfococcus, Desulfofaba, Desulfofrigus, Desulfofustis, Desulfohalobium, Desulfomicrobium, Desulfomonile, Desulfonatronovibrio, Desulfonatronum, Desulfonauticus, Desulfonema, Desulfonispora, Desulfopila, Desulforegula, Desulforhabdus, Desulforhopalus, Desulfosarcina, Desulfospira, Desulfosporosinus, Desulfotalea, Desulfothermus, Desulfotignum, Desulfotomaculum, Desulfovermiculus, Desulfovibrio, Desulfovirga, Desulfovirgula, Desulfurella, Desulfurobacterium, Desulfuromonas, Desulfuromusa, Dethiosulfovibrio, Devosia, Dialister, Diaphorobacter, Dichelobacter, Dichotomicrobium, Dickeya, Dictyoglomus, Dietzia, Diplococcus, Dokdoa, Dokdonella, Dokdonia, Dolosicoccus, Donghaeana, Dorea, Duganella, Dyadobacter, DyeIla, Eberthella, Ectothiorhodospira, Edwardsiella, Eggerthella, Eikenella, Elizabethkingia, Elytrosporangium, Empedobacter, Enhygromyxa, Ensifer, Enterobacter, Enterococcus, Enterovibrio, Epilithonimonas, Eremococcus, Erwinia, Erysipelothrix, Erythrobacter, Erythromicrobium, Escherichia, Ethanoligenens, Eubacterium, Ewingella, Excellospora, Exiguobacterium, Faecalibacterium, Faenia, Falcivibrio, Fastidiosipila, Ferribacter, Ferrimonas, Ferrobacillus, Fervidobacterium, Filibacter, Filifactor, Filobacillus, Filomicrobium, Finegoldia, Flammeovirga, Flavimonas, Flavobacterium, Flectobacillus, Flexibacter, Flexistipes, Flexithrix, Fluoribacter, Fluviicola, Formivibrio, Francisella, Frankia, Frateuria, Friedmanniella, Frigoribacterium, Fulvimarina, Fulvimonas, Fusibacter, Fusobacterium, Gaetbulibacter, Gaffkya, Gallibacterium, Gallicola, Garciella, Gardnerella, Gariaella, Gelidibacter, Gemella, Gemmata, Gemmatimonas, Gemmobacter, Geoalkalibacter, Geobacillus, Geobacter, Geodermatophilus, Geopsychrobacter, Georgenia, Geosinus, Geospirillum, Geothermobacter, Geothrix, Geovibrio, Giesbergeria, Gillisia, Glaciecola, Globicatella, Gluconacetobacter, Gluconoacetobacter, Gluconobacter, Glycomyces, Goodfellowia, Gordona, Gordonia, Gracilibacillus, Gracilibacter, Granulicatella, Granulobacter, Grimontia, Group li D, Guggenheimella, Gulosibacter, Haemophilus, Hafnia, Hahella, Halanaerobacter, Halanaerobium, Haliangium, Haliscomenobacter, Haloactinomyces, Haloanaerobacter, Haloanaerobium, Halobacillus, Halobacteroides, Halocella, Halochromatium, Halococcus, Halolactibacillus, Halomonas, Halonatronum, Halorhodospira, Halothermothrix, Halothiobacillus, Helcococcus, Helicobacter, Heliobacillus, Heliobacterium, Heliophilum, Heliorestis, Herbaspirillum, Herbidospora, Herminiimonas, Herpetosiphon, Hespellia, Hippea, Hirschia, Hoeflea, Holdemania, Holophaga, Hongiella, Hordeomyces, Hyalangium, Hydrocarboniphaga, Hydrogenivirga, Hydrogenobacter, Hydrogenobaculum, Hydrogenomonas, Hydrogenophaga, Hydrogenophilus, Hydrogenothermophilus, Hydrogenothermus, Hydrogenovibrio, Hylemonella, Hymenobacter, Hyphomicrobium, Hyphomonas, Idiomarina, Ignatzschineria, Ignavigranum, Ilyobacter, Inflabilis, Inquilinus, Intrasporangium, lodobacter, Isobaculum, Isochromatium, Isoptericola, Jahnia, Janibacter, Jannaschia, Janthinobacterium, Jensenia, Jeotgalibacillus, Jeotgalicoccus, Jiangella, Jonesia, Kangiella, Kerstersia, Kibdellosporangium, Kibdelosporangium, Kineococcus, Kineosphaera, Kineosporia, Kingella, Kitasatoa, Kitasatospora, Kitasatosporia, Klebsiella, Kluyvera, Knoellia, Kocuria, Kofleria, Koserella, Kozakia, Kribbella, Ktedobacter, Ktedonobacter, Kurthia, Kutzneria, Kytococcus, Labrys, Laceyella, Lachnobacterium, Lachnospira, Lactobacillus, Lactobacterium, Lactococcus, Lactonifactor, Lamprocystis, Lampropedia, Laribacter, Lautropia, Leadbetterella, Lebetimonas, Lechevalieria, Leclercia, Leeuwenhoekiella, Legionella, Leifsonia, Leisingera, Leminorella, Lentibacillus, Lentzea, Leptospirillum, Leptothrix, Leptotrichia, Leucobacter, Leuconostoc, Leucothrix, Levilinea, Levinea, Limnobacter, List, Listeria, Listonella, Loktanella, Lonepinella, Longispora, Lophomonas, Luteibacter, Luteimonas, Luteococcus, Lysobacter, Macrococcus, Macromonas, Magnetospirillum, Mahella, Malikia, Malonomonas, Manjusharmella, Mannheimia, Maribacter, Maricaulis, Marichromatium, Marinibacillus, Marinilabilia, Marinilactibacillus, Marinithermus, Marinitoga, Marinobacter, Marinobacterium, Marinococcus, Marinomonas, Marinospirillum, Marinovum, Marmoricola, Massilia, Mechercharimyces, Mechercharomyces, Megamonas, Megasphaera, Meiothermus, Melittangium, Mesonia, Mesophilobacter, Mesorhizobium, Methanomonas, Methylobacillus, Methylobacter, Methylobacterium, Methylocapsa, Methylocella, Methylocystis, Methylomicrobium, Methylomonas, Methylophaga, Methylophilus, Methylopila, Methylosarcina, Methylotenera, Methylovorus, Microbacterium, Microbispora, Microbulbifer, Microcella, Micrococcus, Microcyclus, Microechinospora, Microellobosporia, Microlunatus, Micromonas, Micromonospora, Micropolyspora, Micropruina, Microscilla, Microstreptospora, Microtetraspora, Microvirgula, Millisia, Mima, Mitsuokella, Mobiluncus, Modestobacter, Moellerella, Mogibacterium, Moorella, Moraxella, Moraxella (Branhamella), Moraxella (Moraxella), Morganella, Moritella, Muricauda, Muricoccus, Myceligenerans, Mycetocola, Mycobacterium, Mycoplana, Myroides, Myxobacter, Myxococcus, Nakamurella, Nannocystis, Natroniella, Natronincola, Nautilia, Naxibacter, Neisseria, Nereida, Nesterenkonia, Nevskia, Nicoletella, Nih Group 12, Nitratifractor, Nitratireductor, Nitratiruptor, Nitrobacter, Nocardia, Nocardioides, Nocardiopsis, Nonomuraea, Novosphingobium, Obesumbacterium, Oceanibulbus, Oceanicaulis, Oceanicola, Oceanimonas, Oceanithermus, Oceanobacillus, Oceanobacter, Oceanomonas, Oceanospirillum, Ochrobactrum, Octadecabacter, Odontomyces, Oenococcus, Oerskovia, Oleiphilus, Oleispira, Oligella, Oligotropha, Olsenella, Opitutus, Orenia, Oribacterium, Ornithinicoccus, Ornithinimicrobium, Ornithobacterium, Oryzihumus, Ottowia, Oxalicibacterium, Oxalobacter, Oxalophagus, Oxobacter, Paenibacillus, Paludibacter, Pandoraea, Pannonibacter, Pantoea, Papillibacter, Parabacteroides, Paracoccus, Paracolobactrum, Paralactobacillus, Paraliobacillus, Parascardovia, Parasporobacterium, Parvibaculum, Parvimonas, Parvopolyspora, Pasteurella, Pasteuria, Patulibacter, Paucibacter, Paucimonas, Paucisalibacillus, Pectinatus, Pectobacterium, Pediococcus, Pedobacter, Pelczaria, Pelobacter, Pelodictyon, Pelomonas, Pelosinus, Pelospora, Pelotomaculum, Peptococcus, Peptoniphilus, Peptostreptococcus, Peredibacter, Persephonella, Persicivirga, Persicobacter, Petrimonas, Petrobacter, Petrotoga, Phaeobacter, Phaeospirillum, Phascolarctobacterium, Phenylobacterium, Phocoenobacter, Photobacterium, Photorhabdus, Phyllobacterium, Phytomonas, Pigmentiphaga, Pilimelia, Pimelobacter, Pirellula, Planctomyces, Planifilum, Planobispora, Planococcus, Planomicrobium, Planomonospora, Planosporangium, Planotetraspora, Plantibacter, Pleomorphomonas, Plesiocystis, Plesiomonas, Podangium, Polaribacter, Polaromonas, Polyangium, Polymorphospora, Pontibacillus, Porphyrobacter, Porphyromonas, Pragia, Prauserella, Prevotella, Proactinomyces, Promicromonospora, Promyxobacterium, Propionibacterium, Propionicimonas, Propioniferax, Propionigenium, Propionimicrobium, Propionispira, Propionispora, Propionivibrio, Prosthecobacter, Prosthecochloris, Prosthecomicrobium, Protaminobacter, polypeptideiphilum, Proteus, Providencia, Pseudaminobacter, Pseudoalteromonas, Pseudoamycolata, Pseudobutyrivibrio, Pseudoclavibacter, Pseudomonas, Pseudonocardia, Pseudoramibacter, Pseudorhodobacter, Pseudospirillum, Pseudoxanthomonas, Psychrobacter, Psychroflexus, Psychromonas, Psychroserpens, Pullulanibacillus, Pusillimonas, Pyxicoccus, Quadrisphaera Rahnella, Ralstonia, Ramibacterium, Ramlibacter, Raoultella, Rarobacter, Rathayibacter, Reinekea, Renibacterium, Renobacter, Rhabdochromatium, Rheinheimera, Rhizobacter, Rhizobium, Rhizomonas, Rhodanobacter, Rhodobacter, Rhodobium, Rhodoblastus, Rhodocista, Rhodococcus, Rhodocyclus, Rhodoferax, Rhodomicrobium, Rhodonellum, Rhodopila, Rhodopirellula, Rhodoplanes, Rhodopseudomonas, Rhodospirillum, Rhodothalassium, Rhodothermus, Rhodovibrio, Rhodovulum, Riemerella, Rikenella, Robiginitalea, Roseateles, Roseburia, Roseiflexus, Roseinatronobacter, Roseobacter, Roseococcus, Roseospira, Roseospirillum, Roseovarius, Rothia, Rubritepida, Rubrivivax, Rubrobacter, Ruegeria, Ruminobacter, Ruminococcus, Runella, Saccharibacter, Saccharococcus, Saccharomonospora, Saccharophagus, Saccharopolyspora, Saccharothrix, Sagittula, Salana, Salegentibacter, Salinibacter, Salinibacterium, Salinicoccus, Salinimonas, Salinispora, Salinivibrio, Salinospora, Salipiger, Salmonella, Samsonia, Sanguibacter, Saprospira, Sarcina, Sarraceniospora, Scardovia, Schlegelella, Schwartzia, Sebekia, Sedimentibacter, Sedimenticola, Segniliparus, Seinonella, Sejongia, Selenomonas, Seliberia, Serinicoccus, Serratia, Shewanella, Shigella, Shinella, Shuttleworthia, Silanimonas, Silvimonas, Simonsiella, Simplicispira, Simsoniella, Sinococcus, Sinorhizobium, Skermania, Slackia, Smaragdicoccus, Smithella, Sodalis, Soehngenia, Sorangium, Sphaerobacter, Sphaerophorus, Sphaerosporangium, Sphaerotilus, Sphingobacterium, Sphingobium, Sphingomonas, Sphingopyxis, Sphingosinicella, Spirilliplanes, Spirillospora, Spirillum, Spirochaeta, Spirosoma, Sporacetigenium, Sporanaerobacter, Sporichthya, Sporobacter, Sporobacterium, Sporocytophaga, Sporohalobacter, Sporolactobacillus, Sporomusa, Sporosarcina, Sporotalea, Sporotomaculum, Stackebrandtia, Staleya, Staphylococcus, Stappia, Starkeya, Stella, Stenotrophomonas, Sterolibacterium, Stigmatella, Streptacidiphilus, Streptimonospora, Streptoallomorpha, Streptoalloteichus, Streptobacillus, Streptobacterium, Streptococcus, Streptomonospora, Streptomyces, Streptomycetoides, Streptomycoides, Streptosporangium, Streptoverticillium, Subdoligranulum, Subtercola, Succiniclasticum, Succinimonas, Succinispira, Succinivibrio, Sulfitobacter, Sulfobacillus, Sulfuricurvum, Sulfurihydrogenibium, Sulfurimonas, Sulfurospirillum, Sutterella, Suttonella, Syntrophobacter, Syntrophobotulus, Syntrophococcus, Syntrophomonas, Syntrophothermus, Syntrophus, Tatlockia, Tatumella, Taxeobacter, Taylorella, Teichococcus, Telluria, Tenacibaculum, Tepidanaerobacter, Tepidibacter, Tepidimicrobium, Tepidimonas, Tepidiphilus, Terasakiella, Terrabacter, Terracoccus, Terribacillus, Terrimonas, Tessaracoccus, Tetragenococcus, Tetrasphaera, Tetrathiobacter, Thalassobacillus, Thalassobacter, Thalassobius, Thalassolituus, Thalassomonas, Thalassospira, Thauera, Thaxtera, Thermacetogenium, Thermaerobacter, Thermanaeromonas, Thermanaerovibrio, Thermicanus, Thermincola, Thermithiobacillus, Thermoactinomyces, Thermoanaerobacter, Thermoanaerobacterium, Thermoanaerobium, Thermoanaerolinea, Thermobacterium, Thermobacteroides, Thermobifida, Thermobispora, Thermobrachium, Thermochromatium, Thermocrinis, Thermocrispum, Thermodesulfatator, Thermodesulfobacterium, Thermodesulfobium, Thermodesulforhabdus, Thermodesulfovibrio, Thermoflavimicrobium, Thermohydrogenium, Thermolithobacter, Thermomicrobium, Thermomonas, Thermomonospora, Thermonema, Thermonospora, Thermopolyspora, Thermosediminibacter, Thermosiculum, Thermosinus, Thermosipho, Thermosyntropha, Thermotoga, Thermovenabulum, Thermovibrio, Thermovirga, Thermus, Thetysia, Thialkalimicrobium, Thialkalivibrio, Thioalkalimicrobium, Thioalkalivibrio, Thiobaca, Thiobacillus, Thiobacter, Thiocapsa, Thiococcus, Thiocystis, Thiodictyon, Thiohalocapsa, Thiolamprovum, Thiomicrospira, Thiomonas, Thiopedia, Thioreductor, Thiorhodoccocus, Thiorhodococcus, Thiorhodovibrio, Thiosphaera, Thiothrix, Thorsellia, Tindallia, Tissierella, Tolumonas, Trabulsiella, Treponema, Trichococcus, Trichotomospora, Truepera, Tsukamurella, Tuberibacillus, Turicella, Turicibacter, Unclassified, Ureibacillus, Uruburuella, Vagococcus, Varibaculum, Variovorax, Veillonella, Verrucomicrobium, Verrucosispora, Vibrio, Victivallis, Virgibacillus, Virgisporangium, Vitreoscilla, Vogesella, Volcaniella, Volucribacter, Vulcanibacillus, Vulcanithermus, Waksmania, Wautersia, Weeksella, Weissella, Williamsia, Wolinella, Woodsholea, Xanthobacter, Xanthomonas, Xenophilus, Xenorhabdus, Xylanibacter, Xylanibacterium, Xylanimicrobium, Xylanimonas, Xylella, Xylophilus, Yania, Yersinia, Yokenella, Zavarzinia, Zimmermannella, Zobellia, Zoogloea, Zooshikella, Zymobacter, Zymobacterium, Zymomonas and Zymophilus.

Plants Comprising Polypeptides According to the Present Invention

The use of the polynucleotides and polypeptides according to the present invention in plant host cells and other transgenic organisms can prevent the loss of valuable crops when the climatic conditions are not optimal for the production of the crops. In particular, the present invention provides novel and innovative, transgenic plants and crops capable of sustaining climatic conditions which cannot be withstood by state-of-the-art plants and crops. Examples of crops in the form of plant host cells according to the present invention comprising polynucleotides and producing polypeptides according to the present invention are are: grapes, oilseed plants, such as canola, grains (oats, barley, rye etc.), citrus fruits, and sugar cane.

The invention is also directed to trans-genic fruits and vegetables comprising the polypeptides according to the present invention. The trans-genic fruits and vegetables comprising the polypeptides according to the present invention are capable of withstanding cooler temperatures e.g. during storage and/or transport. Examples include strawberries, blueberries, raspberries, citrus fruits, bananas, grapes, kiwis, peaches, pineapples, plums, cherries, tomatoes and mangoes.

Flowers Comprising Polypeptides According to the Present Invention

Frozen flowers comptemplated in accordance with the present invention included e.g. tulips, roses, lilies.

Fish

Examples of fish suitable for the invention are Albacore Tuna (Thunnus alalunga), Arrowtooth Flounder (Atheresthes stomias), Atlantic Cod (Gadus morhua), Atlantic Cutlassfish (Trichiurus lepturus), Atlantic Salmon (Salmo salar), Atlantic Wolffish (Anarhichas lupus), Black Drum (Pogonias cromis), Black Pomfret (Parastromateus niger), Blackback Flounder (Sole, Pleuronectes americanus), Blacktip Shark (Carcharhinus limbatus), Catfish (lctalurus furcatus), Crab, Blue (Callinectes sapidus), Marlin (Makaira nigricans), Rockfish (Sebastes auriculatus), Puffer (Sphoeroides annulatus), Scorpionfish (Scorpaena guttata), Sheephead (Semicossyphus pulcher), Rockfish (Sebastes pinniger), Snapper (Lutjanus purpureus), Catfish (lctalurus punctatus), Rockfish (Sebastes goodei, Sebastes nebulosus), Chinook (Oncorhynchus tshawytscha), Chub Mackerel (Scomber japonicus), Coho Salmon (Silver, Medium Red) (Oncorhynchus kisutch), Thresher Shark (Alopias vulpinus), Grouper (Epinephelus fulva), Cusk (Brosme brosme), Mahi-mahi (Coryphaena hippurus), Sole (Microstomus pacificus), Sole (Pleuronectes vetulus), Escolar (Lepidocybium flavobrunneum), Dory (Zeus faber), Ocean Perch (Sebastes norvegicus), Snapper (Lutjanus griseus), Sole (Flounder) (Glyptocephalus cynoglossus), Barracuda (Sphyraena barracuda), Haddock (Melanogrammus aeglefinus), Tuna (Euthynnus affinis), Snapper (Lutjanus synagris), Lingcod (Ophiodon elongatus), Milkfish (Chanos chanos), Tilapia (Tilapia mossambica), Nile Tilapia (Tilapia nilotica), Puffer (Sphoeroides maculatus), Tilefish (Caulolatilus princeps), Oilfish (Ruvettus pretiosus), Orange Roughy (Hoplostethus atlanticus), Barracuda (Sphyraena argentea), (Bonito (Sarda chiliensis), Cod (Alaska Cod, Gadus macrocephalus), Jack (Caranx caninus), Jack (Selene peruviana), Ocean Perch (Sebastes alutus), Mackerel (Scomber scombrus), Spanish (Scomberomorus sierra), Snapper (Lutjanus peru), Patagonian Toothfish (Dissostichus eleginoides), Sole (Flounder, Eopsetta jordani), Pink Salmon (Humpback) (Oncorhynchus gorbuscha), Pollock (Pollachius virens), Rockfish (Sebastes maliger), Trout, Rainbow (Steelhead) (Oncorhynchus mykiss), Drum (Redfish) (Sciaenops ocellatus), Porgy (Chrysophrys auratus), Snapper (Lutjanus campechanus), Rockfish (Sebastes proriger), Sole (Flounder, Errex zachirus), Rockfish (Sebastes aleutianus), Schoolmaster (Lutjanus apodus), Sheepshead (Archosargus probatocephalus), Shark, Mako (Isurus oxyrinchus), Snapper (Lutjanus vivanus), Butterfish (Pampus argenteus), Rockfish (Sebastes brevispinis), Skipjack Tuna (Katsuwonus pelamis), Spinefoot (Siganus favus), Croaker or Corvina (Roncador steamsi), Flounder (Platichthys stellatus), Marlin (Tetrapturus audax), Bass (Morone chrysops x saxatilis), Swordfish (Xiphias gladius), Carp (Barbodes schwanefeldi), Pollock (Alaska Pollock, Theragra chalcogramma), Hake (Urophycis tenuis), Rockfish (Sebastes entomelas), Flounder (Scophthalmus aquosus), Croaker (Yellowfish, Pseudosciaena manchurica), Rockfish (Sebastes ruberrimus), Tuna (Thunnus albacares), Yellowstripe Scad (Selaroides leptolepis), Yellowtail (Seriola lalandei), Flounder (Limanda ferruginea), Rockfish (Sebastes flavidus) and Snapper (Ocyurus chrysurus) Arctic char (Salvelinus alpinus), Turbot, Greenland halibut (Reinhartdius hippoglossoides) Halibut (Hippoglossus hippoglossus).

Frozen Foods and Edible Products

Recrystallisation of frozen food products, including frozen vegetables, leads to a deteriorating taste and texture of such foods. Anti-freeze polypeptides according to the present invention can be used to treat frozen foods or foods to be frozen in order to prevent re-crystallization. Examples of foods for treatment with the invention include, but is not limited to: Ice cream, frozen yoghurt, soups, puddings, sorbets, ice cream bars, frozen desserts e.g. custards, puddings etc and other liquids or semi-liquids for freezing. Frozen vegetables such as celery, potatoes, asparagus, peas, carots, beans, broccoli, sweet corn, spinach, haricots verts etc. is also encompassed by the present invention.

The polypeptides according to the present invention may also affect the formation of lactose crystals. Hence, without being bound by any specific theory, it is believed that the polypeptides according to the present invention inhibit the crystallisation and/or growth of lactose crystals. It is well known that during freezing of ice creams the content of all ingredients (including lactose) is increasingly concentrated except for the content of liquid water, which is decreasing. When the content of lactose reaches a certain level, lactose crystals start to crystallize. This crystallisation is a slow process, which takes place at −20° C. Typically, ice creams are stored at about −20° C. However, in many cases the ice creams are not stored at a stabile and constant temperature, but temperatures fluctuating around −20° C. Hence, during periods where the temperature is higher than −20° C. crystals of lactose will grow and new crystals will be formed. Consequently, the mouth feel of the ice cream will become more coarse, and most people find this mouth feel unpleasant. It has now surprisingly been found that when the polypeptides according to the present invention is added to the ice cream prior to freezing of the ice cream markedly reduces the formation of lactose crystals and markedly prevent that new crystals are formed during storage at about −20° C. Consequently, the quality of the stored ice cream product is markedly improved.

Examples of frozen edible products according to the present invention are disclosed in more detail herein below.

Frozen Fermented Products Comprising the Polypeptide According to the Present Invention

Frozen or refrigerated foods are now a mainstay of the human diet in developed nations. Thus extensive research has and is being carried out by food scientists to ensure high quality products for the consumers. This is particularly true with regard to frozen vegetables and frozen deserts such as ice cream and yogurt.

Frozen deserts such as ice cream or yogurt are generally eaten in the frozen state. Thus, the texture of the frozen product as well as its flavor is important to consumers. Texture is to a large extent governed by the size of the ice crystals. Producers of these frozen deserts have gone to considerable effort and expense to ensure smooth textured products. However, during frozen storage the ice crystals can grow and thus roughen and spoil this texture. The growth of ice crystals during frozen storage is known as recrystallization. This problem is particularly common when the frozen storage conditions are less than ideal, such as during transportation or storage in modern frost-free home freezers. After a relatively short period of time at above-zero temperatures (i.e., above 0° C.), or even at sustained freezing temperatures, frozen foods can become less desirable or even unsuitable for human consumption due to the ice recrystallization process.

An ideal method of incorporating anti-freeze polypeptides into frozen fermented food products is to have the organism responsible for the fermentation process produce the anti-freeze polypeptides while fermenting the food.

Hence, the present invention embraces methods for preparing a frozen fermented food product. This method comprises the steps of (a) contacting a food product with a microorganism that is capable of secreting a polypeptide according to the present invention, wherein the microorganism is capable of fermenting the food product to produce the fermented food product, (b) incubating the food product with the microorganism under conditions in which fermentation takes place so that a fermented food product is produced having the anti-freeze polypeptide present in an amount effective at inhibiting recrystallization of the product; and (c) freezing the fermented food product at a temperature below −5° C., so as to produce a frozen fermented food product.

In one embodiment the food product may be a dairy product (e.g., milk) which can be fermented to produce yogurt, buttermilk or cheese.

The microorganism of the invention is usually a bacterium (e.g., Lactobacillus bulgaricus; Streptococcus cremoris, Streptococcus lactis; Bifidobacterium bifidum, Bifidobacterium longum) but may also be a fungus such as a yeast (e.g., Saccharomyces fragilis, Saccharomyces cerevisiae, Saccharomyces lactis, and others). According to the invention these microorganisms are genetically engineered so that they are capable of secreting a polypeptide according to the present invention.

In a most preferred embodiment the invention comprises incubating milk with bacterial species Lactobacillus balgaricus and Streptococcus lactis that are capable of fermenting milk to produce yogurt and capable of secreting anti-freeze polypeptides; incubating the bacteria and milk under conditions that produce yogurt; and freezing the yogurt at a temperature below −5° C., so as to produce frozen yogurt.

The invention also provides a composition comprising yogurt and a microorganism wherein the microorganism comprises a gene encoding a polypeptide according to the present invention.

As used herein, “fermentation” refers to the chemical conversion of carbohydrates or polypeptides in food products through the use of microorganisms. In this process carbohydrates are often convened to lactic acid.

As used herein “fermented food product;” refers to an edible food product prepared by a process that includes fermentation by a microorganism.

As used herein “yogurt” refers to a dairy product produced by the lactic acid fermentation of milk by the action of microorganisms.

As used herein, “recombinantly produced polypeptides” refers to a polypeptide produced using recombinant DNA techniques. Recombinant DNA techniques are well known and are characterized by the joining of at least two segments of DNA that are not naturally joined in nature (e.g., a bacterial promoter and a polypeptide coding sequence).

The reference sequence may be shorter than the full-length naturally occurring polypeptide or polynucleotide sequence but will be at least 12 residues long for the case of a polypeptide and at least 36 bases long for the case of a polynucleotide.

The present invention also provides methods for preparing a frozen fermented food product by adding a microorganism that is capable of fermenting the food product to produce the fermented food product and also is able to secrete the polypeptide according to the present invention. The use of a microorganism that both secretes the polypeptide according to the present invention and ferments the food product has several advantages over other methods for affecting ice crystal formation and freezing temperature. For example, the claimed method avoids the costly necessity for purifying the polypeptide according to the present invention prior to addition to a food product. In addition, this will eliminate any possible contamination from the purification protocol and the pyrogenicity associated with foreign microorganisms. Furthermore, because the polypeptide according to the present invention is secret by the fermenting microorganism of the claimed invention, this process requires fewer steps than other methods.

The food product of the invention is usually milk but other foods that are fermented to produce an edible fermented food may also be used. Examples include cabbage (which can be fermented to produce sauerkraut), cucumbers (which can be fermented to produce pickles) and soybeans (which can be fermented to produce miso and other products).

In one step of the method, the food product is contacted or mixed with a microorganism capable of fermenting the food product. Examples of microorganisms useful in food fermentation are well known (see, e.g., van de Guchte, 1992, FEMS Microbiology Reviews, 88:73-92).

In a preferred embodiment the food product is milk (e.g., from a cow [i.e. bovine], ewe, mare, or goat). The action of fermenting microorganisms, typically bacteria, on the milk produces yogurt, buttermilk, or certain cheeses, according to the choice of the bacteria and the conditions of incubation. In a most preferred embodiment the method of the invention will be used to produce yogurt from milk. Yogurt is referred to by a variety of names around the world. The names and country of origin of the common varieties of yogurt are listed below:

Product Name Country of Origin Jugurt/Eyran/Ayran Turkey, etc. Busa Turkestan Kissel Mleka Balkans Urgotnic Balkan Mountains Leban/Laban Lebanon/Arab countries Zabady (Zabbady) Egypt/Sudan Mast/Dough Iran/Afghanistan Roba Iraq Dahi/Dadhi/Dahee India Mazun/Matzoon/Matsun/Matsoni Armenia Katyk Transcaucasia Tiaourti Greece Cieddu Italy Mezzoradu Sicily Gioddu Sardinia Biokys Czechoslovakia Karmdinka Poland Tarho Hungary Tykmaelk/Ymer Hungary Villi (Fiili) Finland Filmjolk/Fillbunke/Surmelk/Taettemjolk/ Scandinavia Tettemelk logurte Brazil/Portugal Proghurt Chile Skyr Iceland Gruzovina Yugoslavia Kefir/Donskaya/Varentes Soviet Union Kurunga/Koumiss/Ryazhenka/Guslyanka Tarag Mongolia Shosim/Sho/Thara Nepal

Methods for yogurt production can be found in Functions of Fermented Milk edited by Nakazawa and Hosono, 1992, published by Elsevier Applied Science, London-New York, p. 32, which is incorporated herein by reference. In the United States yogurt is produced from either whole or skim milk from cows. The milk is standardized to 10.5 to 11.5% solids, heated to above 90° C. (30 to 60 minutes) to destroy any contaminating microorganisms, and then cooled. The material is then inoculated with a mixed culture of Streptococcus thermophilus and Lactobacillus bulgaricus in a 1:1 ratio. The combined action of these two organisms is usually needed to obtain the desired flavor and acid in the products. In other instances, other high fermenting bacteria including bulgarian bacteria, L. jugarti, L. acidophilus, Bifido bacterium, spp. Yeast and lactic fungi have also been used. Examples of bacteria and other organisms used for the fermentation of milk to produce yogurt are listed below:

Genus Habit Fermentation Main Species Streptococcus ^(a) Coccal chains Homo S. cremoris, lactis, thermophilus Leuconostoc ^(b) Coccal pairs Hetero L. citrovorum, mesenteroides Lactobacillus ^(c) Rods Homo L. acidophilus, bulgaricus, casei, jugurti, lactis Bifidobacterium Rods Hetero B. bifidum, breve, longum Others: Yeasts (Torulopsis holmil; Saccharomyces fragilis, cerevisiae, lactis; Candida pseudotropicalis, etc.) Fungi (Geotrichum candidum) Acetic acid bacteria (Acetobacter aceti, rasens) ^(a)Now Lactococcus lactis subsp. cremoris, Lac, lactis subsp. lactis and S. thermophilus. ^(b)Now L. mesenteroides subsp. cremoris and L. mesenteroides subsp. mesenteroides. ^(c)Now L. acidophilus, L. delbrueckii subsp. bulgaricus, L. casei subsp. casei, L. helveticus biovar. jugurti and L. delbrueckii subsp. lactis.

The microorganisms may be genetically engineered (i.e., employing the techniques of recombinant DNA technology) so that they are able to secrete the polypeptide according to the present invention.

The methods for engineering bacteria and fungi capable of expressing and secreting a heterologous polypeptide are well established (see, e.g., Maniatis et al. (1982), Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.; Berger and Kimmel, Guide to Molecular Cloning Techniques, Methods in Enzymology 152 (Academic Press, Inc., San Diego, Calif.); Simon et al., 1986, Appl. Environ. Microbiol. 52:394-395; and von Wright et a., 1985, Appl. Environ. Microbiol. 50:1100-1102, all of which are incorporated herein by reference)

The production of microorganisms capable of expressing and secreting an AFP can be carried out in a variety of ways that will be apparent to one of ordinary skill. The DNA sequence encoding the AFP will preferably be operably linked (i.e., positioned to ensure the functioning of) to an operon which allows the DNA to be transcribed (into an RNA transcript) and translated into a polypeptide in the microorganism. Promoters for both bacteria and fungi are well known in the art. Preferred operons for expression in lactic acid bacteria include the lactose operon of S. thermophilus or lac ABCDFEGX operon of L. lactic because they have been used successfully to drive foreign gene expression in the hosts (see, e.g., Simons et al., 1993, J. Bact. 175:5186-5175; Mollet et al., 1993, J. Bact. 175:4315-4324).

The polypeptide according to the present invention may be expressed as a fusion polypeptide for increased stability or other beneficial properties. Furthermore the polypeptide according to the present invention may be modified via a modification of the gene encoding the polypeptide. In general, modifications of the genes may be readily accomplished by a variety of well-known techniques, such as site-directed mutagenesis (see, e.g., Gillman and Smith, 1979, Gene 8:81-97 and Roberts et al., 1987, Nature 328:731-734).

The microorganisms of the invention are capable of secreting the polypeptide according to the present invention. Accordingly, the polypeptide according to the present invention will preferably be linked to a signal peptide sequence. Examples of suitable signal peptide sequences include those from the usp45 gene of L. lactis ssp lactis MG 1363 and the L. lactis ssp cremoris SK11 cell envelop associated protease gene (van Asseldonk et al., 1990, Gene 95:155-160; De vos et al., 1989, J. Dairy Sci. 72:3398-3405). For bacteria such as L. lactis the usp45 signal peptide is preferred since it derives from the same host. In one preferred embodiment the polypeptide gene according to the present invention is linked to a transcription termination sequence to ensure correct termination of transcription of the polypeptide according to the present invention in the host system.

A gene construct including elements described above is constructed using plasmids such as pUC19, pNZ18 and pDBN183 as vectors (Solaiman et al., 1992, Plasmid, 28:25-36). The gene construct is incorporated into the genome of a lactic acid bacterial species using homologous recombination techniques (Mollet et al., 1993, J. Bact., 175:4315-4324). The lactic acid bacteria and E. coli strains can be maintained as recommended by Maniatis et al. in Molecular Cloning, A Laboratory Manual, supra; and Chagnand et al., 1992, Can. J. Microbiol. 38:67-74.

Microorganisms comprising the polypeptide according to the present invention may be applied to food products in any conventional way. In the case of products such as milk, the bacteria or fungus can be mixed intimately with the food product that is to be fermented and frozen. It will be known by those of skill that mixtures of different microorganisms are sometimes used to produce the desired product. For example, in preparation of yogurt, S. thermophilus and L. bulgaricus are often used together.

The number of FAE microorganisms added to the food product will depend on the properties of the microorganisms and of the food. Generally, lactic acid FAE starter bacteria (10¹⁰-10¹¹ per ml) are incubated at 1-5% into pasteurized and cooled milk such that the proportion results in an appropriate amount of polypeptide according to the present invention in the product. The amount of AFP in the product should be an amount effective at preventing or inhibiting ice recrystallization (1-100 mg/liter milk). This can be determined using the splat-cooling assay described by Knight et al. (1988) Cryobiology, vol. 25, pp. 55-60.

In another step of the method, the fermented food product is frozen using conventional freezer operations, such as blast freezers (−20 to 40° C.) or contact plate freezers (−300 to 40° C.) or vacuum freeze driers. It will be apparent to one of ordinary skill that numerous variations of the aforementioned embodiments are possible.

Ice Cream Comprising the Polypeptide According to the Present Invention

In another aspect, the present invention provides an ice cream product comprising a polypeptide according to the present invention. The ice cream product can also comprise an emulsifier system together with a polypeptide according to the present invention. This emulsifier system may be any system known by the skilled person. However, systems comprising mono esters of propane-1,2-diol and fatty acids, such as the ones described in US 2005/0163902 or WO 2008/064675, are particular preferred.

In the ice cream manufactured as described herein below, the polypeptide according to the present invention may be added as a purified polypeptide, mixed with other ingredients during manufacture of the ice cream or the polypeptide according to the present invention may be present as a result of secretion from the microorganism used for fermenting the milk.

One way of manufacturing the ice cream according to the present invention is as follows.

In a first step a food intermediate is contacted with the above mentioned emulsifier system.

By the term “food intermediate” as used herein is meant a mixture of ingredients suitable for preparing the ice cream. Ingredients suitable for preparing ice cream may include water, fat such as milkfat or vegetable fat, milk solids not fat (MSNF), sweeteners, stabilisers, flavourings and colurings. Furthermore, the polypeptide according to the present invention may already be present in the milk solid, or it may be added to the mixture as a separate ingredient.

Preferably, the food intermediate comprises fat. Preferably the fat is a high lauric fat or milk fat. By the term “high lauric fat” is meant a fat in which the predominant fatty acid is lauric acid. High lauric fats, such as hardened palm kernel oil and hardened coconut oil, are β′ stable. Hence, preferably the food intermediate comprises β′ stable fats.

After the first contact step, the selected ingredients are mixed together. Typically the liquid ingredients are mixed together first and the dry ingredients are added subsequently. The liquid ingredients may be cold or may be heated to approximately 60° C. Blending requires rapid agitation to incorporate powders and often high speed blenders are used.

If butter/butter oil or vegetable fat is used, it should ideally be melted separately and added to the mix at 40° C. or via a static mixer at the entrance of the homogeniser by means of a dosing pump.

The mix is subsequently pasteurised. Pasteurisation is carried out to destroy pathogenic bacteria and spoilage organisms such as psychrotrophs. There are three distinct stages in pasteurization: pasteurization, homogenisation and cooling

Homogenisation of the mix is carried out in order to form the fat emulsion by breaking down or reducing the size of the fat globules found to less than 1 μm.

Pasteurisation may be carried out by continuous pasteurisation or batch pasteurisation.

Today the most common pasteurisation principle applied is continuous pasteurisation where the ice cream mix is typically heated for a minimum of 16 seconds at a temperature ranging from 80-90° C. in a plate heat exchanger. Continuous pasteurisation is usually performed in a high temperature short time (HTST) heat exchanger following blending of ingredients in a large, insulated feed tank. Some preheating, to 30° C. to 40° C., may be necessary for solubilisation of the components.

The HTST system is equipped with heating sections, cooling sections, and regenerative sections.

Batch pasteurisation is the old method where all mix ingredients are slowly heated in a vat equipped with a hot water jacket. In order to avoid fouling on the bottom and sides of the vat, the heating process has to be gentle with a low differential temperature (delta T) between the mix and the heating medium. As the delta T has to be low and the ratio of mix volume/vat surface is typically high, it will inevitably take several minutes just to heat the mix to a temperature of 60° C. Effective agitation of the mix is needed in order to improve the transfer of heat from the vat surface to the mix. Energy consumption for batch pasteurisation is very high and, unlike continuous pasteurisation, there is no heat recovery.

Following pasteurisation, the mix is homogenised by means of high pressures. Homogenisation typically takes place at a temperature of about 80° C. and the homogenisation pressure can be in the region of 90 bar (1300 psi) to 250 bar (3600 psi) at a temperature of 65-75° C. Batch tanks are usually operated in tandem so that one is holding while the other is being prepared. Automatic timers and valves ensure the proper holding time has been met.

Homogenisation can be carried out either before or after pasteurisation.

Subsequently the mix is cooled to refrigerated temperatures (4° C.) by passing it across a heat exchanger (plate or double or triple tube).

The mixture is cooled to the aging temperature which is about 4° C. The mix is then aged for a minimum of four hours but preferably overnight. This allows time for the fat to crystallize and for the polypeptides and polysaccharides to fully hydrate.

Following aging, the mix may be drawn into a flavour tank where any liquid flavours, fruit purees, or colours are added. The mix then enters the dynamic freezing process which both freezes a portion of the water and whips air into the frozen mix. Freezing may be carried out by a continuous freezing process or by batch freezing/whipping.

Continuous freezing may be carried out in a barrel freezer. The barrel freezer is a scraped-surface, tubular heat exchanger, which is jacketed with a boiling refrigerant such as ammonia or freon. The mix is pumped through the barrel freezer and is drawn off the other end in about 30 seconds to 3 minutes. In the case of batch freezers the process takes 10 to 15 minutes. When the mix is drawn off the other end about 50% of its water is frozen. There are rotating blades inside the barrel freezer that keep the ice scraped off the surface of the freezer. There are also dashers inside the machine which help to whip the mix and incorporate air.

Ice cream contains a considerable quantity of air, typically up to half of its volume. This gives the product its characteristic lightness. The air content is termed its overrun.

As the ice cream is drawn with about half of its water frozen, particulate matter such as fruit pieces, nuts or cookies, may be added to the semi-frozen slurry. The ice cream is then packaged and is placed into a blast freezer at −30° to −40° C. where most of the remainder of the water is frozen.

Hardening involves static (still, quiescent) freezing of the packaged products in blast freezers. The freezing rate should ideally be rapid, so freezing techniques involve low temperature (−40° C.) with either enhanced convection (freezing tunnels with forced air fans) or enhanced conduction (plate freezers).

Instead of a traditional hardening process the ice cream may be pumped from the ice cream freezer into a low temperature extruder (single or double screw extruder) which brings the temperature of the ice cream down to −12° C. to −18° C. After filling or extrusion the ice cream may be taken directly into cold storage.

The hardened ice cream should be stored below −25° C. Below about −25° C., ice cream is quite stable for long time without danger of fast ice crystal growth.

As previously mentioned, the process of the present invention comprises the step of contacting a food intermediate with an emulsifier system.

In one preferred embodiment, the process comprises the step of dissolving the emulsifier system in water. In this embodiment the emulsifier system may be dissolved in water and the food intermediate may then be contacted with water.

In one preferred embodiment, the process comprises the step of dissolving the emulsifier system in fat. In this embodiment the emulsifier system may be dissolved in fat and the food intermediate may then be contacted with fat.

In one preferred embodiment the process comprises a dynamic freezing step.

The term “dynamic freezing step” as defined herein means subjecting the food intermediate to freezing conditions whilst agitating the food intermediate. This is in contrast to a quiescent freezing step in which the food intermediate is subjected to freezing conditions whilst static.

In one preferred embodiment the process comprises a freezing step.

In one preferred embodiment the process comprises a freezing step with a drawing temperature from the freezer lower than −4° C. Preferably the drawing temperature from the freezer is about −4° C. to −7° C., preferably about −5° C. to −7° C., more preferably about −5° C. to −6° C., more preferably about −6° C. The drawing temperature is the temperature of the ice cream as it exits the ice cream freezer.

Aerated Food Products Comprising the Polypeptide According to the Invention

The present invention also provides an aerated food product comprising a polypeptide according to the invention. Ice cream, sherbet, sorbet and frozen yoghurt can be mentioned as examples of food products, which may be characterized as aerated products.

The term “aerated” means that gas has been intentionally incorporated into a mix, for example by mechanical means. The gas can be any gas, but is preferably, in the context of food products, a food-grade gas such as air, nitrogen, nitrous oxide, or carbon dioxide. The aerated food products of the invention comprise a population of gas bubbles, wherein at least 65% of the gas bubbles have a diameter of less than 20 μm. Preferably at least 75%, more preferably at least 80% of the gas bubbles have a diameter of less than 20 μm. Preferably at least 50%, more preferably at least 60%, most preferably at least 75% of the gas bubbles have a diameter of less than 10 μm.

The extent of aeration is typically defined in terms of “overrun”. In the context of the present invention, % overrun is defined in terms of the volume of the aerated product and the volume of the unaerated mix from which it was formed:

${Overrun} = \frac{\begin{pmatrix} {{{Volume}\mspace{14mu} {of}\mspace{14mu} {final}\mspace{14mu} {aerated}\mspace{14mu} {product}} -} \\ {{volume}\mspace{14mu} {of}\mspace{14mu} {unaerated}\mspace{14mu} {mixture}} \end{pmatrix} \times 100}{{Volume}\mspace{14mu} {of}\mspace{14mu} {unaerated}\mspace{14mu} {mixture}}$

Overrun is measured at atmospheric pressure. The amount of overrun present in the product will vary depending on the desired product characteristics

Preferably the food product has an overrun of at least 20%, more preferably at least 50%, most preferably at least 80%. Preferably the food product has an overrun of at most 400%, more preferably at most 200%, most preferably at most 120%.

The aerated food product may be manufactured by use of any process known in the art, such as for example by use of the pre-aeration route, which is a process for producing aerated food products comprising a large proportion of small gas bubbles starting from an unaerated mix.

In the pre-aeration route, a mix (i.e. an aqueous solution and/or suspension) comprising the polypeptide according to the present invention and optionally other ingredients, is subjected to an aeration step. The aeration step must be of a sufficiently high “intensity” so that a large number of very small gas bubbles (less than 20 μm in diameter) are created. The intensity of the aeration process depends on a number of factors, the most important of which are the rate of energy dissipation in the aeration step, the nature of the flow experienced by the mix and the gas bubbles in the aeration step, and the viscosity and temperature of the mix. In addition, the aeration step should be long enough to achieve the desired degree of aeration (i.e. overrun).

Mechanical aerating devices are often based on a rotor which shears the mix. The rate of energy dissipation is a function of the speed of rotation of the device. Generally speaking, a high rate of energy dissipation (and hence a high rotational speed) is required to produce small gas bubbles (see for example “Chemical Engineering for the Food Industry”, Fryer, Pyle and Rielly, Blackie, London, 1997).

The effectiveness of the aeration step also depends on the nature of the flow in the aerating device. Aeration is normally achieved by initially incorporating relatively large gas bubbles which are subsequently broken up into smaller ones. Elongational flow or extensional flow is known to be particularly effective at breaking up large gas bubbles, compared to simple shear flow (see e.g. Rallinson, J. M. Ann. Rev. Fluid Mech. 16, pp 45-66, 1984). Suitable high shear aeration processes and devices that can provide at least a component of elongational flow include: continuous whipping in a rotor-stator device such as an Oakes mixer (E.T. Oakes Corp), a Megatron mixer (Kinematica AG), a Mondo mixer (Haas-Mondomix BV) or a Silverson mixer (Silverson Machines Inc.); gas injection followed by mixing and dispersion in a continuous flow device such as a scraped surface heat exchanger; and batch whipping involving surface entrainment of gas, using e.g. a Hobart whisk mixer (Hobart UK), Kenwood Chef mixer (Kenwood Ltd), Ultra-Turrax mixer (IKA Werke GmbH & Co. KG) or an electrical hand-held mixer, for example a Breville kitchen hand blender.

The effectiveness of the aeration step also depends on the viscosity and/or the temperature of the mix. By increasing the viscosity and/or lowering the temperature of the mix, the size reducing effect of the aeration device on the gas bubbles is increased. Furthermore, the effectiveness of the aeration step also depends on the formulation of the mix.

Although the effectiveness of the aeration step depends on the specific details of the process and apparatus used and the mix being aerated, it is within the compass of one skilled in the art to determine the appropriate process conditions in any particular situation, by considering the factors described above. In particular, the proportion of very small gas bubbles can be increased by increasing the energy dissipated and/or by increasing the elongational flow component and/or by increasing the viscosity of the mix and/or by lowering the temperature of the mix.

The aerated mixture may optionally be subjected to freezing during and/or after aeration, for example if the final product is to be a frozen aerated product such as an ice cream or a sorbet. Freezing may take place simultaneously with aeration, for example in a scraped surface heat exchanger. Simultaneous freezing and aeration can aid the formation of small gas bubbles because of the increase in the mix viscosity as ice forms. When freezing takes place after aeration, it is preferably carried out so that little or no further gas is incorporated.

The ice content may be increased further by subsequent freezing operations, such as low-temperature extrusion, placing the aerated mixture in a mould immersed in a bath of cold liquid such as brine or glycol, dropping portions of the aerated mixture directly into a bath of cryogenic fluid such as liquid nitrogen or placing a container comprising the aerated mixture into a cold environment such as a freezer, blast freezer or cold store. The subsequent freezing step is preferably carried out at low or zero shear so that little or no further gas is incorporated.

In addition to a polypeptide according to the invention, the aerated food products of the invention (and the mixtures from which they are made) may comprise other ingredients conventionally found in food products, such as fats, milk or cream; oil or fat, notably in the form of an emulsified phase; sugars, salt, fruit and/or vegetable material, extract, juice, thickeners, such as polysaccharides, stabilisers, colours, flavours, chemical emulsifiers, such as monoglycerides; acids and preservatives. Preferred food products include ice cream, sorbet, mousse, whipped cream, aerated beverages such as milk shakes and smoothies, low-fat spreads (e.g. having a fat content of 0-60 wt %), dressings and sauces. Preferably the food product is a frozen or chilled aerated confection such as ice cream, sorbet or mousse.

Frozen aerated confections of the invention comprise the polypeptide according to the present invention and optionally one or more anti-freeze polypeptides other than the polypeptide according to the invention. In aerated products the amount of the total of anti-freeze polypeptides is typically at least about 0.0001 wt %, more preferably at least 0.0005 wt %, most preferably at least 0.001 wt %. Anti-freeze polypeptides can be used at very low concentrations and therefore preferably the confections comprise less than 0.05 wt % Anti-freeze polypeptides. A preferred range is from about 0.001 to 0.01 wt %. Anti freeze polypeptides can be used individually or in combination with other anti freeze polypeptides known in the area.

Frozen aerated products may optionally comprise coatings, such as a layer of chocolate or couverture and/or inclusions, such as nuts, fruit, toffee or chocolate pieces. In this case, the fat content of the frozen aerated confection does not include fat present in the coating or inclusion.

In one embodiment, the frozen aerated confection comprises 3 wt % or less fat, preferably 2 wt % or less, more preferably 1 wt % or less. In a preferred embodiment, the confection is fat-free, which means that the confection comprises substantially no fat (i.e. less than 0.1 wt %).

Aerated Food Products Comprising the Polypeptide According to the Invention Together with Hydrophobin and a Surfactant

The present invention also provides a frozen aerated food product, such as a confection, comprising a polypeptide according to the invention together with hydrophobin and a surfactant. Preferably the aerated food product comprises a population of gas bubbles, wherein at least 65% of the gas bubbles have a diameter of less than 20 μm.

The amount of hydrophobin present in the frozen aerated confection will generally vary depending on the confection formulation and volume of the air phase. Typically, the confection will comprise at least 0.001 wt %, hydrophobin, more preferably at least 0.005 or 0.01 wt %. Typically the confection will comprise less than 1 wt % hydrophobin. The hydrophobin can be from a single source or a plurality of sources e.g. the hydrophobin can be a mixture of two or more different hydrophobin polypeptides.

The hydrophobin is added in a form and in an amount such that it is available to stabilise the air phase. By the term “added”, is meant that the hydrophobin is deliberately introduced into the confection for the purpose of taking advantage of its foam stabilising properties. Consequently, where ingredients are present or added that comprise fungal contaminants, which may comprise hydrophobin polypeptides, this does not constitute adding hydrophobin within the context of the present invention.

Typically, the hydrophobin is added to the confection in a form such that it is capable of self-assembly at an air-liquid surface.

Typically, the hydrophobin is added to the confections of the invention in an isolated form, typically at least partially purified, such as at least 10% pure, based on weight of solids. By “added in isolated form”, is meant that the hydrophobin is not added as part of a naturally-occurring organism, such as a mushroom, which naturally expresses hydrophobins. Instead, the hydrophobin will typically either have been extracted from a naturally-occurring source or obtained by recombinant expression in a host organism.

In one embodiment, the hydrophobin is added to the confection in monomeric, dimeric and/or oligomeric (i.e. consisting of 10 monomeric units or fewer) form. Preferably at least 50 wt % of the added hydrophobin is in at least one of these forms, more preferably at least 75, 80, 85 or 90 wt %. Once added, the hydrophobin will typically undergo assembly at the air, liquid interface and therefore the amount of monomer, dimer and oligomer would be expected to decrease.

The term “surfactant” (or “surface active agent”) as used herein means a substance which lowers the surface tension of the medium in which it is dissolved and, accordingly, positively adsorbs at the liquid/vapour interfaces.

The term includes sparingly soluble substances which lower the surface tension of a liquid by spreading spontaneously over its surface. In the context of the present invention, the term “surfactant” does not include hydrophobins.

The term “surfactant” does not include trace quantities of surface active components that may be present in very small amounts in another (non-surface active) ingredient, for example stabilisers such as pectins, locust bean gum, and guar gum. In such cases, the amount of surfactant would normally be less than 0.05% by weight of the food product.

The surfactant is typically an ingredient which is used in aerated food products because of its beneficial effect on taste and/or texture. Such surfactants include (but are not limited to):

-   -   milk polypeptides such as caseins, whey (and their polypeptide         fractions), sodium caseinate, calcium caseinate, and hydrolysed         whey polypeptides;     -   other polypeptides such as gelatine, egg polypeptides, and soy         polypeptide;     -   mono- and di-glycerides of saturated or unsaturated fatty acids,         e.g. monoglyceryl palmitate;     -   polyoxyethylene derivatives of hexahydric alcohols (usually         sorbitol), glycols, glycol esters, polyglycerol esters, sorbitan         esters, stearoyl lactylate, acetic acid esters, lactic acid         esters, citric acid esters, acetylated monoglyceride, diacetyl         tartaric acid esters, polyoxyethylene sorbitan esters (such as         polysorbate 80);     -   non-ionic surfactants such as alkyl poly(ethylene oxide), fatty         alcohols, and sucrose esters;     -   phospholipids and mixtures of phospholipids (e.g. lecithin); and         mixtures of any the above.

Preferably the surfactant is present in an amount of at least 0.05% by weight of the product, more preferably at least 0.1%. Preferably the surfactant is a polypeptide, more preferably milk polypeptide, and is present in an amount of at least 0.5% by weight of the food product, more preferably at least 1%. Preferably the surfactant is present in an amount of at most 20% by weight of the food product, more preferably at most 10%, most preferably at most 5%.

The aerated food products according to the present invention may be produced by use of the “pre-aeration” route (disclosed above in further detail), which is a process for producing aerated food products comprising a large proportion of small gas bubbles starting from an unaerated mix comprising hydrophobin and surfactant. Another route, termed “post-addition” provides a process whereby the surfactant is added after aeration.

The post-addition route provides a way in which the amount of hydrophobin can be increased in relation to the amount of surfactant at the point at which the bubbles are formed whilst it remains unchanged in the final product, by adding the surfactant after aeration has taken place. Thus a mix comprising hydrophobin but not surfactant is aerated; subsequently a second mix comprising the surfactant is combined with the aerated mix. The second mix is formulated so that the combined mixes give the desired final product formulation. A mixing step may be used to improve the homogeneity of the combined mixes. The mixing step is preferably carried out at relatively low shear and for short times so that little or no further gas is incorporated (i.e. the overrun does not increase by more than 10% during the mixing step). Suitable mixing devices include: static mixers; in-line dynamic mixers such as an auger, blender or fruit-feeder; and batch mixing devices, such as a stirred vessel. In a batch process, the second (surfactant-comprising) mix would typically be injected near the end of the processing period. The mixing step could also take place in a continuous process, for example in a scraped surface heat exchanger or screw extruder by injecting the second mix into the barrel of the scraped surface heat exchanger or screw extruder close to the point at which the product exits.

The aerated mixture may optionally be subjected to freezing during and/or after aeration, for example if the final product is to be a frozen aerated product such as an ice cream or a sorbet. Freezing may take place simultaneously with aeration, for example in a scraped surface heat exchanger. Simultaneous freezing and aeration can aid the formation of small gas bubbles because of the increase in the mix viscosity as ice forms. When freezing takes place after aeration, it is preferably carried out so that little or no further gas is incorporated. When the surfactant is added after aeration (i.e. the post-addition route) freezing may take place before and/or during the mixing step. The surfactant stream may be chilled or partially frozen before mixing.

Frozen Water Confections Comprising the Polypeptide According to the Present Invention

Typically, frozen water confections are relatively small, for example having an average volume of less than 1 ml, more preferably less than 0.5 ml. By way of example, beads having a diameter of from 5 mm to 10 mm would have a volume of from about 0.065 ml to about 0.5 ml. Typically the discrete frozen confections have a minimum average volume such that each confection can be readily distinguished by a consumer. For example, the discrete frozen confection preferably has a minimum average volume of at least about 0.02 ml.

The discrete frozen water confections may be made to any shape, such as in the form of cubes or spheres. Preferably, the frozen confections are substantially spherical.

The frozen water confections may be in the form of a composite product where at least one portion or region of the product, such as a core or layer, does not contain the polypeptide according to the present invention. An example of this would be a product containing a core of ice cream which lacks the polypeptide according to the present invention, coated in a layer of water ice that does contain the polypeptide according to the present invention. Preferably, substantially the outer layer of the composition confection comprises the polypeptide according to the present invention, i.e. the region which will come into contact with other discrete frozen confections. It will be appreciated that in the case of a composite product, the wt % amount of the polypeptide according to the present invention added to the confection is calculated solely in relation to those components of the confection that contain the polypeptide according to the present invention and not in relation to the complete product.

Frozen water confections may be aerated or unaerated. By unaerated is meant a frozen confection having an overrun of less then 20%, preferably less than 10%. An unaerated frozen confection is not subjected to deliberate steps such as whipping to increase the gas content. Nonetheless, it will be appreciated that during the preparation of unaerated frozen confections, low levels of gas, such as air, may be incorporated in the product.

Water ice confections typically contain sugar, water, colour, fruit acid or other acidifying agent, fruit or fruit flavouring and stabiliser. Preferably, the total solids content is at least 6 wt %, more preferably at least 8 wt % or at least 10, 12, 15 or 20 wt % and may be as high as about 35 wt %. Preferably the total solids content is less then 35 wt %, more preferably less than 25 wt %. Water ices may be aerated or unaerated. If aerated, the overrun is typically less than about 50%, for example from about 25% to 30%. In one embodiment, the water ice confections of the invention are unaerated.

Preferably the water ice confections comprise less than 2 wt % artificial sweeteners, more preferably less than 1 wt %. In a highly preferred embodiment, no artificial sweeteners, such as aspartame or acesulfame are present in the water ice confections.

Frozen water confections of the invention typically comprise one or more stabiliser, such as one or more stabilisers selected from gums, agar, alginates and derivatives thereof, gelatin, pectin, lecithin, sodium carboxymethylcellulose, carrageenan and furcelleran. Preferably a blend of stabilisers is used, such as blend of a gum and carrageenan. In a preferred embodiment, the frozen confection comprises from 0.1 to 1 wt % stabiliser.

Frozen water confections of the invention typically comprise at least about 0.0005 wt of the polypeptide according to the present invention. The polypeptides according to the present invention can be used at very low concentrations and therefore preferably the confections comprise less than 0.05 wt % of the polypeptide according to the present invention. A preferred range is from about 0.001 to 0.01 wt %.

Frozen water confections of the invention can be manufactured using a number of techniques known in the art. For example, free-flowing beads can be manufactured by dispensing drops of the liquid mix into a freezing chamber of liquid nitrogen (see WO96/29896). Other shapes can be manufactured by moulding techniques, for example by introducing a liquid premix into a cooled mould. Moulded products may contain complex shapes and have a high degree of surface definition.

Ice cream-containing products and the like need not be subjected to a cold hardening step of below from −20° C. to −25° C., although this may be used if desired, especially if the product is a composite product with a layer or core that does not contain the polypeptide according to the present invention.

The frozen water confectionery product of the invention may be packaged in containers for sale to consumers as an individual unit. The volume of such containers is typically from 100 ml to 1000 ml, such as from 200 ml to 500 ml.

However, the product can also be packaged in larger containers for retail purposes where the product is dispensed into smaller containers at the retail premises, e.g. in fast food outlets or as a pick ‘n’ mix format where consumers can choose from frozen confections of the invention having different shapes, flavours and/or colours. These larger containers may, for example, have a volume greater than about 1000 ml, for example at least 2000 ml or 5000 ml.

Discrete Frozen Dairy Confection Comprising the Polypeptide According to the Present Invention

The present invention also provides a frozen confectionary product comprising a plurality of discrete unaerated dairy frozen confection being able to contact directly other discrete frozen confections in the product.

Ice confections are sweet-tasting fabricated foodstuffs intended for consumption in the frozen state (i.e. under conditions wherein the temperature of the foodstuff is less than 0° C., and preferably under conditions wherein the foodstuff comprises significant amounts of ice). Ice confections of the present invention comprise from 1 to 8 wt % fat and have a total solids content of from 10 to 25 wt %. These amounts of fat and total solids, in combination with a water-soluble aerating gas and the polypeptide according to the present invention, result in products having both the desired texture and appearance. Typical water ice formulations (which do not contain fat) and standard ice cream formulations (which have a total solids content of at least about 30 wt %) do not fall within the definition of discrete frozen dairy confection.

The ice confections of the present invention preferably comprise from 2 to 6 wt %, more preferably from 2.5 to 5 wt % fat. The fat may come from any suitable source, such as for example butterfat, coconut oil, palm oil, cocoa butter, sunflower oil, olive oil or rapeseed oil, and mixtures or fractions thereof.

The total solids content of an ice confection is the dry weight of the confection, i.e. the sum of the weights of all the ingredients other than water, expressed as a percentage of the total weight. It is measured as described in Ice Cream, 6th Edition, p 296. The ice confections of the present invention have a total solids content of from 10 to 25 wt % of the ice confection. Preferably the total solids content is at least 12%, more preferably at least 15%, most preferably at least 18%. Preferably the total solids content is at most 24% more preferably at most 22%.

Ice confections according to the present invention contain ice. Since the total solids content is from 10 to 25 wt %, the water content is correspondingly from 90 to 75 wt %. At a temperature of −18° C. most, but not all, of the water is frozen.

Ice confections of the invention typically comprise at least about 0.0001 wt % of the polypeptide according to the present invention, more preferably at least 0.0005 wt %. The polypeptides according to the present invention can be used at very low concentrations and therefore preferably the confections comprise less than 0.05 wt % of the polypeptide according to the present invention. A preferred range is from about 0.001 to 0.01 wt %, more preferably from 0.005 to 0.01 wt %.

An aerating agent refers to any component which because of its surface activity and/or the viscosity it imparts, aids the formation of small gas bubbles and resists their coalescence or separation. The aerating agent is to be understood not to include the aerating gas. Preferably the aerating agent is a polypeptide-based aerating agent, for example a hydrolysed milk polypeptide such as Hygel™ and Hyfoama™ (available from Kerry Biosciences); or a hydrolysed soya polypeptide such as Versawhip (available from Kerry Biosciences) and D-100™ (available from Gunter Industries). Alternatively, the aerating agent may be non-protein-based, for example a monoglyceride, such as Myverol 18-04K (a distilled 95% monoglyceride prepared from vegetable oils, available from Quest International), or a polyglycerol ester, such as PGE 55 (a polyglycerol ester of fatty acids, available from Danisco). The amount of aerating agent in the confection is at least 0.1 wt %, preferably at least 0.15 wt %.

Preferably the amount of aerating agent is less than 0.5 wt %, preferably less than 0.4 wt %, more preferably less than 0.25 wt %.

Ice confections of the invention may comprise stabiliser. Stabilisers include polypeptides such as gelatin; plant extrudates such as gum arabic, gum ghatti, gum karaya, gum tragacanth; seed gums such as locust bean gum, guar gum, tara gum, psyyllium seed gum, quince seed gum or tamarind seed gum; konjac mannan; seaweed extracts such as agar, alganates, carrageenan or furcelleran; pectins such as low methoxyl or high methoxyl-type pectins; cellulose derivatives such as sodium carboxymethyl cellulose, microcrystalline cellulose, methyl and methylethyl celluloses, or hydroxylpropyl and hydroxypropylmethyl celluloses; and microbial gums such as dextran, xanthan or β-1,3-glucan. The stabiliser may be a single stabiliser, or a mixture of two or more stabilisers. Preferably, the stabiliser is locust bean gum. The amount of stabiliser is preferably at most 0.3 wt %, more preferably at most 0.25 wt %. For example, the amount of stabiliser is typically from 0 to 0.2 wt %.

Ice confections of the invention may contain polypeptide (in addition to any polypeptide based aerating agent), preferably in an amount of at least 1 wt %, more preferably at least 1.5 wt %. Ice confections containing at least this amount of polypeptide are perceived as milk ice-type products and are more attractive to many consumers than substantially polypeptide-free ice confections. Preferably the polypeptide content is less than 8 wt %, more preferably less than 6 wt %, most preferably less than 3 wt %. Suitable polypeptides for use in the present invention include milk polypeptides, egg polypeptides and gelatine as well as vegetable polypeptides such as soya polypeptides. Particularly preferred are milk polypeptides owing to their superior flavour and heat stability. Suitable sources of milk polypeptide include milk, concentrated milk, milk powders, whey, whey powders and whey polypeptide concentrates isolates.

Ice confections of the invention typically comprise sugars e.g. sucrose, fructose, dextrose, lactose, corn syrups, sugar alcohols; they may also contain other ingredients, for example colours and flavours.

The ice confection preferably has an overrun of at least 20%, more preferably at least 40%, most preferably at least 60%. Preferably the overrun is at most 150%, more preferably at most 120%, most preferably at most 120%.

“Mix” refers to the unaerated mix prior to aeration (or following de-aeration of the melted ice confection). Overrun is measured at atmospheric pressure.

The ice confection containing of the invention may constitute an entire product or may be a component of a composite product. In a composite product the ice confection of the invention provides contrast in texture and appearance to the other component(s) of the product. Preferably such composite products contain the ice confection as a discrete element in their structure. For example, a relatively soft ice cream core can be coated with a layer of the ice confection to provide a hard, crispy layer surrounding the ice cream core. Another example is the incorporation of the ice confection as inclusions. Alternatively the ice confection may be provided with a continuous or partial coating of, for example, a water glaze, a non-aerated water ice or chocolate on at least one surface. In a composite product the determination of the total solids and the fat, aerating agent, ice structuring polypeptide, stabiliser, and polypeptides contents takes into account only the ice confection, and not other components of the composite product.

Discrete frozen dairy confection comprising the polypeptide according to the present invention may be prepared by any suitable method known in the art. Preferably, however, the discrete frozen dairy confection is manufactured by the method comprising the steps of:

-   (a) preparing a mix of ingredients; then -   (b) pasteurising and homogenising the mix; then -   (c) adding the polypeptide according to the present invention -   (d) simultaneously freezing and aerating the mix with an aerating     gas which contains at least 50% by volume of a carbon dioxide,     nitrous oxide or mixtures thereof to produce the ice confection (for     example in an ice cream freezer); -   (e) cold hardening the ice confection, wherein step (c) may take     place before, during or after step (b).

The mix is aerated with a gas containing at least about 50% by volume of carbon dioxide, nitrous oxide or mixtures thereof, preferably at least about 70%, more preferably 100%. The remainder of the aerating gas will typically be a nitrogen-containing gas such as air. Most preferably the aerating gas is 100% carbon dioxide.

After freezing, the resulting ice confection may be shaped e.g. by extrusion followed by cutting or by moulding, prior to the cold hardening step. Preferably the ice confection is extruded at a temperature of from 4° to −1.5° C., more preferably from −2.5 to −1.5° C. Relatively high extrusion temperatures result in a particularly good foam-like appearance.

Preferably the cold hardening step takes place at a temperature of about −25° C. or below, for example by blast freezing. After cold hardening, the ice confections are preferably stored at a temperature in the range of −25 to −10° C., typically about −18° C.

Low Fat Dairy Products Comprising the Polypeptide According to the Invention

The present invention also provides a frozen, low fat dairy product. Frozen dairy confections are confections that typically contain milk or milk solids, such as ice cream, milk ice, frozen yogurt and sherbet. The term “milk” includes milk substitutes such as soya milk, although mammalian milk is preferred. Preferably the frozen dairy confection is an ice cream or milk ice.

The low fat product of the present invention preferably comprises 3 wt % or less fat, preferably 2 wt % or less, more preferably less than 2 wt %, or 1 wt % or less. In one embodiment, the product is fat-free, which means that the product comprises substantially no fat (i.e. less than 0.1 wt %). Where the product is coated with a non-dairy composition such as a chocolate or couverture layer, the determination of fat content for the product should disregard the coating.

Frozen confections containing milk preferably contain at least about 3 wt % milk solid non-fat (MSNF), more preferably from about 5 wt % to about 25 wt % MSNF.

Stabilisers may be present in the frozen products of the invention although it should be noted that the stabilising effects of the polypeptides according to the present invention can allow for stabiliser replacement in some cases. However, significant levels of stabilisers may still be required, in addition to polypeptides according to the present invention, in some product formulations, such as very low fat products with less than 1 wt % fat, to produce the desired product stability. Nonetheless, the resulting products are improved over previous products because the polypeptide according to the present invention reduces or ameliorates the deleterious effects of the stabilisers on texture and taste.

Suitable stabilisers include alginates, gelatin, gum acacia, guar gum, gum karaya, locust bean gum, carageenan and salts thereof, xanthan gum, microcrystalline cellulose, cellulose ethers or mixtures thereof. The amount of stabiliser is preferably 1.5% or less by weight, more preferably 1% or less by weight such as from 0.1 to 0.8 wt %.

In one embodiment, the product comprises at least 0.5 wt % stabilisers, such as at least 0.7 wt % stabilisers. Preferably the level of fat in such a product is less than 2 or 1 wt %. In another embodiment the product comprises less than 0.5 wt % stabilisers. Preferably the level of fat in such as product is at least 1 wt % or more, more preferably at least 2 wt %.

Frozen confections of the invention typically comprise at least about 0.0001 wt % of the polypeptide according to the present invention, more preferably at least 0.0005 wt %. The polypeptides according to the present invention can be used at very low concentrations and therefore preferably the confections comprise less than 0.05 wt % polypeptide according to the present invention. A preferred range is from about 0.001 to 0.01 wt %, more preferably from 0.005 to 0.01 wt %.

The frozen confections may be aerated or unaerated, preferably aerated. By unaerated is meant a frozen confection having an overrun of less then 20%, preferably less than 10%. An unaerated frozen confection is not subjected to deliberate steps such as whipping to increase the gas content. Nonetheless, it will be appreciated that during the preparation of unaerated frozen confections, low levels of gas, such as air, may be incorporated in the product. The amount of overrun present in an aerated product will vary depending on the desired product characteristics. For example, the level of overrun in ice cream is typically from about 70 to 100%, and in confectionery such as mousses the overrun can be as high as 200 to 250 wt %, whereas the overrun in milk ices is from 25 to 30%. Aerated frozen confections preferably have an overrun of from 30% to 200%, more preferably from 50% to 150%.

Frozen confections of the invention can be manufactured using a variety of techniques known in the art. Products are typically frozen quiescently or using agitation, such as in a surface-scraped heat exchanger. Products may be moulded. Products may contain complex shapes and have a high degree of surface definition since the addition of the polypeptide according to the present invention preserves the stability of such shapes and structures.

The polypeptides according to the present invention can be added prior to, during or after freezing of the product. If added after freezing, this will take place whilst the product is still plastic so that the polypeptide according to the present invention can be mixed e.g. after extrusion from a surface-scraped heat exchanger and prior to hardening.

Ice cream products and the like can be subjected to an optional cold hardening step of below from −20° C. to −25° C.

The present invention also encompasses compositions for producing a low fat frozen confectionery product of the invention, which composition comprises the polypeptide according to the present invention, preferably at least 0.005 wt % of the polypeptide according to the present invention. Such compositions include liquid premixes and dry mixes, for example powders, to which an aqueous liquid, such as milk or water, is added.

Frozen Food Products Designed for Thawing in a Microwave, Said Products Comprising the Polypeptide According to the Present Invention

Freezing is a very common technique for preserving food. With certain notable exceptions, frozen food is usually thawed prior to use or further processing (e.g., cooking). Thawing is accomplished satisfactorily by leaving the frozen food product to stand at ambient temperature. However, even on a domestic scale, the length of time taken to accomplish satisfactory thawing is considerable. Thawing is also accomplished on an industrial scale by the application of conductive or convective heat to the frozen food product. However, the apparatus needed to accomplish such thawing is not readily available to the consumer.

Microwave ovens are increasingly widespread in both an industrial and domestic context. One of their uses is in the thawing of frozen food. Microwave thawing is more rapid than thawing at ambient temperature. It still suffers from a number of disadvantages:

-   -   the low thermal diffusivity of frozen food necessitates the use         of pulsed microwaves to allow temperature equilibrium to be         established;     -   liquid water absorbs microwave energy much more readily than         ice, tending to result in “hotspots” and uneven thawing;     -   the geometry of the food item with regard to size and shape must         be suitable;     -   because of the necessity of using only intermittent microwave         pulses, the time to thaw a food item completely is considerable.

It has been found that if a composition comprising a mesophase of water, emulsifier and the polypeptide according to the present invention is incorporated into a food product and if at least an amount of the water is present as unfrozen water in the frozen food product, an improved product is obtained.

The word mesophases herein includes both layered structures and traditional mesophases i.e. lamellar, cubic, hexagonal (1 and 2), L2 and L1 and also dispersed mesophases i.e. liposomes, cubosomes and hexosomes. Additionally, it includes the formation of micelles, which will also form such surfaces.

It has been found that the above described frozen food product may be thawed uniformly and rapidly by the application of direct microwave energy, without the necessity of using intermittent or pulsed microwaves.

It is believed that the ability of the systems of the present invention to maintain a proportion of unfrozen water when present in a frozen food product is due to the ability of the compositions to form mesophases. Mesophases are structures where the polar emulsifier and water are organised in a well-defined structure according to their polarity. The polar end group of the emulsifier is in contact with the water phase or phases. A number of different mesophase structures are believed to exist. The water close to the polar end group of the emulsifier is organised in such a way that it is protected from freezing.

The ratio of water to emulsifier in the composition of the invention will depend on the emulsifier used, and the particular application of the composition. It has been found that for any particular emulsifier/water system, the amount of liquid water present below 0° C. (“unfrozen water”) tends to increase with the proportion of water up to a maximum. Up to this maximum point, it is thought that substantially all the water in the system is unfrozen. Beyond this point, a fixed amount of the water present is unfrozen, with the balance frozen.

Preferably, the compositions of the invention comprise at least an amount of unfrozen water when present in a frozen food product at a temperature of −15° C. or below.

Preferably, the compositions of the invention comprise at least an amount of unfrozen water when present in a frozen food product at a temperature of −20° C. or below.

Preferably, the compositions of the invention comprise at least an amount of unfrozen water when present in a frozen food product at a temperature of about −25° C.

Preferably, the compositions of the invention comprise at least an amount of unfrozen water when present in a frozen food product at a temperature of about −40° C.

When present in a frozen food product, the compositions of the present invention preferably comprise an amount of unfrozen water that is thermodynamically stable at temperatures below 0° C.

Preferably, the water component is present in an amount of at least 0.1% based on the total weight of the composition. Preferably, the water component is present in an amount of at least 1% based on the total weight of the composition. Preferably, the water component is present in an amount of at least 2% based on the total weight of the composition. Preferably, the water component is present in an amount of at least 3% based on the total weight of the composition. Preferably, the water component is present in an amount of at least 5% based on the total weight of the composition. Preferably, the water component is present in an amount of at least 10% based on the total weight of the composition.

Preferably, the water component is present in an amount of at most 99.9% based on the total weight of the composition. Preferably, the water component is present in an amount of at most 50% based on the total weight of the composition. Preferably, the water component is present in an amount of at most 40% based on the total weight of the composition. Preferably, the water component is present in an amount of at most 30% based on the total weight of the composition. Preferably, the water component is present in an amount of at most 25% based on the total weight of the composition.

Preferably, the water component is present in an amount of between 0.1 and 99.9% based on the total weight of the composition. More preferably, the water component is present in an amount of between 1 and 25% based on the total weight of the composition.

Preferably, the emulsifier is present in an amount of at least 0.1% based on the total weight of the composition. Preferably, the emulsifier is present in an amount of at least 50% based on the total weight of the composition. Preferably, the emulsifier is present in an amount of at least 60% based on the total weight of the composition. Preferably, the emulsifier is present in an amount of at least 70% based on the total weight of the composition. Preferably, the emulsifier is present in an amount of at least 80% based on the total weight of the composition. Preferably, the emulsifier is present in an amount of at least 99.0% based on the total weight of the composition. Preferably, the emulsifier is present in an amount of at least 99.9% based on the total weight of the composition.

Preferably, the emulsifier is present in an amount up to 99.9% based on the total weight of the composition. Preferably, the emulsifier is present in an amount up to 99% based on the total weight of the composition. Preferably, the emulsifier is present in an amount up to 97% based on the total weight of the composition. Preferably, the emulsifier is present in an amount up to 95% based on the total weight of the composition. Preferably, the emulsifier is present in an amount up to 90% based on the total weight of the composition.

Preferably, the emulsifier is present in an amount of between 0.1 and 99.9% based on the total weight of the composition. More preferably, the emulsifier is present in an amount of between 75 and 90% based on the total weight of the composition.

Preferably, the polypeptide according to the invention is present in an amount of at least 0.001% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at least 0.01% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at least 0.1% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at least 1% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at least 5% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at least 10% based on the total weight of the composition.

Preferably, the polypeptide according to the invention is present in an amount of at most 90% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at most 50% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at most 25% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at most 15% based on the total weight of the composition. Preferably, the polypeptide according to the invention is present in an amount of at most 10% based on the total weight of the composition.

Preferably, the polypeptide according to the invention is present in an amount of between 0.001 and 90% based on the total weight of the composition. More preferably, the polypeptide according to the invention is present in an amount of between 0.01 and 10% based on the total weight of the composition.

In a preferred aspect, the composition comprises less than 25% w/w of oil. More preferably, the composition comprises less than 10% w/w of oil. More preferably, the composition comprises less than 5% w/w of oil. More preferably, the composition comprises less than 1% w/w of oil. Still more preferably the composition comprises less than 0.1% w/w of oil. Most preferably, the composition comprises substantially no oil.

Other components may also be present in the compositions of the invention, provided that they do not affect the ability to retain at least an amount of unfrozen water when present in a frozen food product.

An example of a technique of bringing into association is mixing. Mixing of water with the polypeptide according to the invention and an emulsifier may be achieved by any one of a number of means that will be apparent to one skilled in the art. Mixing in an electric mixer is one example.

If ingredients additional to polypeptide according to the invention, an emulsifier and water are present in the composition, then these may be incorporated at any appropriate stage.

Preferably, the food product comprises an amount of the composition sufficient that the amount of unfrozen water present in the food product as a whole enables uniform and rapid microwave thawing. In practice, this equates to an amount of at least 0.1% w/w of unfrozen water present in the food product as a whole.

The usage level will depend on the specific food product, the application and how much water that will be needed to preserve the food texture after freezing.

An amount of non frozen water as low as around 0.1% of the total product gives a product that rapidly and uniformly thaws when heated in a microwave oven. This even thawing results in food products with improved textural properties. To obtain 0.1% of unfrozen water according to this invention takes approximately 0.20% of PGE. The exact amount of emulsifier will depend on the nature of the emulsifier, and may readily be determined by one skilled in the art. For example, 0.14% of Dimodan® M090 or 0.14% of Grindsted® PGE 070 (Danisco, Denmark) will produce the same effects.

Preferably, the food product comprises the composition of the invention in an amount of at least 0.1% w/w. Preferably, the food product comprises the composition of the invention in an amount of at least 0.2% w/w. Preferably, the food product comprises the composition of the invention in an amount of at least 0.3% w/w. Preferably, the food product comprises the composition of the invention in an amount of at least 0.4% w/w. Preferably, the food product comprises the composition of the invention in an amount of at least 0.5% w/w.

Preferably, the food product comprises the composition of the invention in an amount of less than 10% w/w. Preferably, the food product comprises the composition of the invention in an amount of less than 5% w/w. Preferably, the food product comprises the composition of the invention in an amount of less than 4% w/w. Preferably, the food product comprises the composition of the invention in an amount of less than 3% w/w.

Preferably, the food product comprises the composition of the invention in an amount of between 0.1 and 5% w/w, more preferably between 0.5 and 3% w/w.

The mode of application of the composition of the invention to the food product will depend on the nature of the food product in question. For instance, if the food product is liquid or semiliquid at ambient temperature, the composition may be incorporated simply by mixing it with the food product.

In some embodiments of the invention, the water, the polypeptide according to the invention and emulsifier may be added to the food product separately. Water may be added followed by the polypeptide according to the invention and emulsifier; alternatively the polypeptide according to the invention and emulsifier may be added, followed by water.

It is preferred that the polypeptide according to the invention, the emulsifier and water are combined before addition to the food product.

Alternatively, the composition may be incorporated at any point during the food preparation process. For example, the composition may be sprayed on to the surface of the food product. The composition may be injected in to the food product (e.g. in the case of poultry, meat or fish).

The skilled person will be able to judge when to best achieve this incorporation.

Preferably, the food product is selected from low fat spread, mayonnaise, yoghurt, bakery fillings, margarine, reconstituted fruits, jams, fruit preparations, fruit fillings, ripples, fruit sauces, stewed fruit, coffee whitener, instant fruit dessert, confectionery (such as marsh mallow), potato based foods (such as chips, french fries and croquettes), prepared meals (such as casseroles and stews) and fine foods (such as dressings including salad dressings; ketchup, vinaigrette dressings and soups). The food product may be a beverage, raw, processed or pasteurised foods including raw meat, cooked meat, raw poultry products, cooked poultry products, raw seafood products, cooked seafood products, [raw or cooked meat, poultry and seafood products], sausages, frankfurters, ready to eat meals, pasta sauces, pasteurised soups, marinades, oil-in-water emulsions, water-in-oil emulsions, cheese spreads, processed cheese, dairy desserts, flavoured milks, cream, fermented milk products, cheese, butter, condensed milk products, cheese spreads, pasteurised liquid egg, ice cream mixes, soya products, pasteurised liquid egg, confectionery products, fruit products, and foods with fat-based or water-containing fillings. The food product may be a bakery product such as bread, cakes, fine bakery and dough.

Cosmetic and Dermatological Compositions According to the Present Invention

The present invention also provides a cosmetic or dermatological preparation which comprises the polypeptide according to the present invention—optionally in combination with one or more additional polypeptides which are selected from anti-freezing polypeptides and anti-freezing glycoproteins.

The preparation may comprise only the polypeptide according to the present invention or the preparation may comprise at least one additional anti-freezing polypeptide. Furthermore the composition may comprise at least one anti-freezing glycoprotein together with the polypeptide according to the present invention.

In the preparation the polypeptide according to the present invention in the preparation may be present in a concentration of from 0.0001% to 50% by weight, based on the total weight of the preparation, e.g., in a concentration of from 0.001% to 50% by weight, of from 0.1% to 10% by weight, or from 0.1% to 1% by weight.

In cases where one or more additional polypeptides selected from anti-freezing polypeptides and anti-freezing glycoproteins are also present in the preparation together the polypeptide according to the present invention total amount of polypeptide may amount to from 0.0001% to 50% by weight, based on the total weight of the preparation, e.g., in a concentration of from 0.001% to 50% by weight, of from 0.1% to 10% by weight, or from 0.1% to 1% by weight.

Preferably, the at least one additional anti-freezing polypeptide may comprise at least one polypeptide selected from types AFP 1, AFP 2, AFP 3 and AFP 4, for example, at least one polypeptide of type AFP 1 that is synthesized by pseudopluronectes americanus, myoxocephalus scorpius, myoxocephalus aenaeus and/or myoxocephalus scorpiodes, at least one polypeptide of type AFP 2 that is synthesized by hemitripterus americanus, osmerus mordax and/or clupea harengus harengus, at least one polypeptide of type AFP 3 that is synthesized by macrozoarces americanus, rhigophila dearbomi lycodes polaris and/or the “wolf fish” anarhichas lupus, and/or at least one polypeptide of type AFP 4 that is synthesized by myoxocephalus octodecimspinosis. Preferably, the at least one anti-freezing glycoprotein may comprise at least one polypeptide that is synthesized by trematomas borgrevinki, dissostichus mawsoni, boreogadus saida and/or gadus morhua.

In one aspect of the present invention, at least a part of the one or more polypeptides in the preparation may be encapsulated.

The present invention also provides a cosmetic or dermatological preparation which comprises the polypeptide according to the present invention and one or more polypeptides which are selected from anti-freezing polypeptides and anti-freezing glycoproteins that are synthesized by at least one of pseudopluronectes americanus, myoxocephalus scorpius, myoxocephalus aenaeus, myoxocephalus scorpiodes, hemitripterus americanus, osmerus mordax, clupea harengus harengus, macrozoarces americanus, rhigophila dearborni, lycodes polaris, anarhichas lupus, myoxocephalus octodecimspinosis, trematomas borgrevinki, dissostichus mawsoni, boreogadus saida and gadus morhua.

Preferably, the total amount of polypeptide according to the present invention in the cosmetic or dermatological preparation amounts to from 0.001% to 50% by weight, based on the total weight of the preparation, e.g., in a concentration of from 0.1% to 10% by weight. Preferably, the total amount of polypeptide in the cosmetic or dermatological preparation may amount to from 0.001% to 50% by weight, based on the total weight of the preparation, e.g., in a concentration of from 0.1% to 10% by weight.

The present invention also provides a cosmetic or dermatological product which is an o/w cream, a w/o cream, a w/o/w cream, an o cream, a w/o emulsion, a hydrodispersion, a gel cream, a w/o stick or an o stick, and which comprises the preparation of the present invention, including the various aspects thereof.

The present invention also provides a method for the treatment or prevention of undesirable skin conditions. The method comprises applying the polypeptide according to the present invention and optionally one or more polypeptides to at least parts of the skin, which polypeptides are selected from anti-freezing polypeptides and anti-freezing glycoproteins.

In one aspect, the undesirable skin conditions may include skin inflammation, pigmentation disorders, symptoms of extrinsic and intrinsic skin aging and/or skin damage caused by UV radiation.

In the technical filed of cosmetic and dermatologic preparations, the term “anti-freezing polypeptides” is used to describe polypeptides that enable an organism, even under extreme temperature conditions, to keep important cell structures functionally active. In view of their function, “anti-freezing polypeptides” in this sense also represent “frost-protection compounds” on a cellular level.

It was not foreseeable for those of skill in the art that the preparations according to the present invention protect better against structural and cellular damage in the skin due to cold better maintain or restore the barrier properties of the skin better combat drying out of the skin act better against dyschromia act better against inflammatory skin conditions act better against skin aging, and better protect the skin against environmental influences than the preparations of the prior art.

The use of a polypeptide according to the present invention optionally together with additional anti-freezing polypeptides (AFP) and/or anti-freezing glycoproteins (AFGP) or cosmetic or topical dermatological preparations with an effective content of the polypeptide according to the present invention optionally together with additional AFP and/or AFGP renders possible an effective treatment, but also a prophylaxis of structural and cellular damage in the skin due to cold, which damage with distinct climate- and weather-induced drops in temperature cause changes in the cell physiology in the cell and in the extracellular space through loss of the temperature optima of cellular enzymes,skin damage, skin redness and tight feeling of the skin and increased sensory sensitivities, induced, e.g., by cold, wind and/or UV light, temperature-sensitive skin, negative changes in the skin, the lips and the mucous membranes in the nose and mouth area and the integumentary appendage caused by environmental stress (caused by temperature changes and UV light, smoking, smog, reactive oxygen species, free radicals).

The use of the polypeptide according to the present invention optionally together with additional AFP and/or AFGP or the use of cosmetic or topical dermatological preparations with an effective content of the polypeptide according to the present invention optionally together with additional AFP and/or AFGP is an effective treatment as well as a prophylaxis of deficient, sensitive or hypoactive skin conditions or deficient, sensitive or hypoactive conditions of integumentary appendages of signs of premature aging of the skin (e.g., wrinkles, senile keratoses, telangiectases) and/or of the integumentary appendages, of environmentally induced (smoking, smog, reactive oxygen species, free radicals) and in particular light-induced negative changes in the skin and the integumentary appendages, of light-induced skin damage, of pigmentation disorders, of sensitive, irritated and itchy skin, of dry skin conditions and disorders of the horny layer barrier, of hair loss and for improved hair growth, signs of skin aging, such as, e.g., wrinkles and reduced skin regeneration, of inflammatory skin conditions, and atopic eczema, seborrhoeic eczema, polymorphous photodermatosis, psoriasis, vitiligo, to sooth sensitive or irritated skin, to stimulate the synthesis of collagen, hyaluronic acid and elastin, changes of the normal hyaluronic acid and glycosaminoglycan content of healthy skin, to stimulate the ceramide synthesis of the skin to stimulate intracellular DNA synthesis, in particular in cases of deficient or hypoactive skin conditions, to increase cell renewal and regeneration of the skin, to increase the skin's own protective and repair mechanisms (for example, for dysfunctional enzymes, DNA, lipids, polypeptides), reduction in cell-cell communication deficient, sensitive or hypoactive skin conditions or deficient, sensitive or hypoactive conditions of skin appendages, a change in the ceramide, lipid and energy metabolism of healthy skin, changes in lipid and polypeptide peroxidation, a change in the physiological transepidermal water loss, a reduction in skin hydration, normal osmoregulation and decrease in the moisture content of the skin, change in the natural moisturizing factor content, DNA damage and reduction in endogenous DNA repair mechanisms, activation of metalloproteinases and/or other proteases or inhibition of the corresponding endogenous inhibitors of these enzymes, deviations from the normal post-translational modifications of connective tissue constituents of healthy skin, dandruff formation in the hair and hair region, brittleness of the skin, loss of elasticity and skin fatigue, increase in the normal keratinocyte proliferation, reduction of the natural regeneration and structure of the skin and hair for pre- and post-treatment in cases of topical application of laser and abrasive treatments, which serve, for example, to reduce skin wrinkles and scars, to counteract the resulting skin irritations and to promote the regeneration processes in the damaged skin.

Accordingly, the use of the polypeptide according to the present invention optionally together with additional AFPs and/or AFGPs for the prophylaxis and treatment of inflammatory skin conditions-also atopical eczema-and/or for skin protection in the case of skin predisposed to be sensitive and dry is also in accordance with the invention.

Accordingly, the use of cosmetic or dermatological preparations for the production of cosmetic or dermatological preparations for the treatment and/or prophylaxis of pigmentation disorders is also in accordance with the invention.

Accordingly, the use of preparations for the production of cosmetic or dermatological preparations for the treatment and/or prophylaxis of the symptoms of intrinsic and/or extrinsic skin aging and for the treatment and prophylaxis of harmful effects of ultraviolet radiation on the skin is also according to the invention.

Hence, the use of the polypeptide according to the present invention optionally together with additional AFPs and/or AFGPs for the production of cosmetic or dermatological preparations for increasing ceramide biosynthesis is also an aspect of the invention.

Furthermore, the use of AFPs and/or AFGPs for the production of cosmetic or dermatological preparations for strengthening the barrier function of the skin is yet another aspect of the invention.

Cosmetic or dermatological preparations according to the present invention preferably contain from 0.0001% to 50% by weight, particularly preferably from 0.01% to 10% by weight, of the cited the polypeptide according to the present invention optionally together with additional AFPs and/or AFGPs or a combination of two or more of the cited AFPs and/or AFGPs, based on the total weight of the preparations.

According to the present invention, customary antioxidants can be used in the preparations that contain the active substance combinations according to the present invention.

Advantageously, the antioxidants are selected from the group of amino acids (for example, glycine, histidine, tyrosine, tryptophan, [beta]-alanine) and derivatives thereof, imidazoles (for example urocanic acid) and derivatives thereof, peptides, such as D,L-carnosine, D-carnosine, L-carnosine and derivatives thereof (for example, anserine), carotenoids, carotenes (for example, [alpha]-carotene, [beta]-carotene, lycopene) and derivatives thereof, lipoic acid and derivatives thereof (for example, dihydrolipoic acid), aurothioglucose, propylthiouracil and other thiols (for example, thioredoxin, glutathione, cysteine, cystine, cystamine and the glycosyl, N-acetyl, methyl, ethyl, propyl, amyl, butyl and lauryl, palmitoyl, oleyl, [gamma]-linoleyl, cholesteryl and glyceryl esters thereof) and salts thereof, dilauryl thiodipropionate, distearyl thiodipropionate, thiodipropionic acid and derivatives thereof (esters, ethers, peptides, lipids, nucleotides, nucleosides and salts) and sulfoximine compounds (for example, buthionine sulfoximines, homocysteine sulfoximine, buthionine sulfones, penta-, hexa- and heptathionine sulfoximine) in very low tolerated doses (for example pmol to μmol/kg), and furthermore (metal) chelating agents (for example, [alpha]-hydroxy fatty acids, palmitic acid, phytic acid, lactoferrin), [alpha]-hydroxy acids (for example, citric acid, lactic acid, malic acid), humic acid, bile acid, bile extracts, bilirubin, biliverdin, EDTA, EGTA and derivatives thereof, unsaturated fatty acids and derivatives thereof (for example [gamma]-linolenic acid, linoleic acid, oleic acid), folic acid and derivatives thereof, alanine diacetic acid, flavonoids, polyphenols, catechols, vitamin C and derivatives thereof (e.g., ascorbyl palmitate, Mg-ascorbyl phosphate, ascorbyl acetate), tocopherols and derivatives thereof (for example, vitamin E acetate), and coniferyl benzoate of benzoin resin, rutinic acid and derivatives thereof, ferulic acid and derivatives thereof, butylated hydroxytoluene, butylated hydroxyanisole, nordihydroguaiacic acid, nordihydroguaiaretic acid, trihydroxybutyrophenone, uric acid and derivatives thereof, mannose and derivatives thereof, zinc and derivatives thereof (for example, ZnO, ZnSO₄), selenium and derivatives thereof (for example selenomethionine), stilbenes and derivatives thereof (for example stilbene oxide, trans-stilbene oxide) and the derivatives of these active ingredients mentioned which are suitable according to the invention (salts, esters, ethers, sugars, nucleotides, nucleosides, peptides and lipids).

The amount of the antioxidants (one or more compounds) in the preparations is preferably from 0.001% to 30% by weight, particularly preferably from 0.05% to 20% by weight, particularly preferred from 1% to 10% by weight, based on the total weight of the preparation.

In addition, it may be advantageous to encapsulate the active ingredients according to the invention, as so-called solid lipid nanoparts using molten waxes, which may be chosen, inter alia, but not exclusively, from ester waxes, triglyceride waxes or hydrocarbon waxes. In addition, it may be advantageous to encapsulate the active ingredients according to the invention in polymers, e.g., in particles based on highly crosslinked polymethacrylates and/or cellulose triacetates and/or as core/shell particles with a shell made of poly(oxymethylurea), nylon, polyamides, polyurethane, polyester, gelatin and polyolefins.

The prophylaxis or the cosmetic or dermatological treatment with the active ingredient used according to the invention or with the cosmetic or topical dermatological preparations having an effective content of active ingredient used according to the invention may be carried out in the usual manner, by applying the active ingredient used according to the invention or the cosmetic or topical dermatological preparations having an effective content of active ingredient used according to the invention to the affected areas of the skin.

The active ingredient used according to the invention can advantageously be incorporated into customary cosmetic and dermatological preparations which may assume various forms. Thus, they may, for example, be a solution, an emulsion of the water-in-oil (W/O) type or of the oil-in-water (O/W) type, or a multiple emulsion, for example of the water-in-oil-in-water (W/O/W) type or oil-in-water-in-oil (O/W/O) type, a hydrodispersion or lipodispersion, a gel, a Pickering emulsion, a solid stick or an aerosol.

Emulsions according to the invention for the purposes of the present invention, e.g., in the form of a cream, a lotion, a cosmetic milk, and a stick, are advantageous and may comprise, for example, fats, oils, waxes and/or other fatty substances, and water and one or more emulsifiers as are customarily used for this type of formulation.

It is also possible and advantageous for the purposes of the present invention to incorporate the active ingredient used in accordance with the present invention into aqueous systems or surfactant preparations for cleansing and treating the skin and the hair.

One of skill in the art is, of course, aware that demanding cosmetic compositions are almost inconceivable without the customary auxiliaries and additives. Examples thereof include builders, fillers, perfume, dyes, emulsifiers, additional active ingredients, such as vitamins or polypeptides, light protection agents, stabilizers, insect repellents, alcohol, water, salts, and antimicrobially, proteolytically or keratolytically active substances, etc.

Corresponding requirements apply mutatis mutandis to the formulation of medicinal preparations.

Medicinal topical compositions for the purposes of the present invention generally comprise one or more medicaments in an effective concentration. For the sake of simplicity, for a clear distinction between cosmetic and medicinal application and corresponding products, reference is made to the legal provisions of the Federal Republic of Germany (e.g., Cosmetics Directive, Foods and Drugs Act).

In this connection, it is likewise advantageous to add the active ingredient used according to the invention as an additive to preparations which already comprise other active ingredients for other purposes.

Accordingly, for the purposes of the present invention, cosmetic or topical dermatological compositions can, depending on their formulation, be used, for example, as skin protection cream, cleansing milk, sunscreen lotion, nourishing cream, day or night cream, lip care stick, nasal spray, etc. In some instances it is possible and advantageous to use the compositions according to the invention as bases for pharmaceutical formulations.

It is also advantageous for the purposes of the present invention to provide cosmetic and dermatological preparations whose main purpose is not protection against sunlight, but which nevertheless have a content of UV protection substances. Thus, for example, UVA and/or UVB filter substances are usually incorporated into day creams or makeup products. Also UV protection substances, likewise antioxidants and, if desired, preservatives, provide an effective protection of the preparations against deterioration. Furthermore, cosmetic and dermatological preparations are favorable which are in the form of a sunscreen.

Accordingly, the preparations according to the present invention, in addition to one or more active ingredient combinations according to the invention, preferably additionally comprise at least one further UVA filter substance and/or UVB filter substance. The formulations can, although this is not necessary, optionally also comprise one or more organic and/or inorganic pigments as UV filter substances, which can be present in the aqueous phase and/or the oil phase.

Preferred inorganic pigments are metal oxides and/or other metal compounds which are insoluble or sparingly soluble in water, in particular the oxides of titanium (TiO₂), zinc (ZnO), iron (e.g., Fe₂O₃), zirconium (ZrO₂), silicon (SiO₂), manganese (e.g. MnO), aluminum (Al₂O₃), cerium (e.g., Ce₂O₃), mixed oxides of the corresponding metals, and mixtures of such oxides.

According to the invention such pigments can advantageously be surface-treated (“coated”) whereby, e.g., an amphiphilic or hydrophobic character of these pigments is to be formed or retained. This surface treatment can comprise providing the pigments with a thin hydrophobic layer by methods known per se.

According to the invention, e.g., titanium dioxide pigments are advantageous that are coated with octylsilanol. Suitable titanium dioxide particles are available under the trade name T805 from Degussa. Furthermore, TiO₂ pigments coated with aluminum stearate are particularly advantageous, e.g., those available under the trade name MT 100 T from TAYCA.

A further advantageous coating of the inorganic pigments comprises dimethylpolysiloxane (also: dimethicone), a mixture of completely methylated, linear siloxane polymers which are terminally blocked with trimethylsiloxy units. For the purposes of the present invention, particularly advantageous pigments are zinc oxide pigments which are coated in this way.

Also advantageous is a coating of the inorganic pigments with a mixture of dimethylpolysiloxane, in particular dimethylpolysiloxane having an average chain length of from 200 to 350 dimethylsiloxane units, and silica gel, which is also referred to as simethicone. It is particularly advantageous if the inorganic pigments have been additionally coated with aluminium hydroxide or hydrated aluminium oxide (also alumina, CAS No.: 1333-84-2). Particularly advantageous are titanium dioxides which have been coated with simethicone and alumina, it being possible for the coating to also comprise water. One example thereof is the titanium dioxide available under the trade name Eusolex T2000 from Merck.

An advantageous organic pigment for the purposes of the present invention includes 2,2′-methylenebis-(6-(2H-benzotriazol-2-yl)-4-(1,1,3,3-tetramethylbutyl)phenol) [INCI: Bisoctyltriazole], which is obtainable from CIBA Chemikalien GmbH under the trade name Tinosorb(R) M.

Advantageously, preparations according to the invention contain substances that absorb UV radiation in the UVA and/or the UVB range, whereby the total amount of the filter substances is, e.g., from 0.1% by weight to 30% by weight, preferably from 0.5 to 20% by weight, in particular from 1.0 to 15% by weight, based on the total weight of the preparations, in order to provide cosmetic preparations that protect the hair or the skin against the entire range of ultraviolet radiation. They can also be used as sunscreen for the hair or the skin.

Further advantageous UVA filter substances for the purposes of the present invention include dibenzoylmethane derivatives, in particular 4-(tert-butyl)-4′-methoxydibenzoylmethane (CAS No. 70356-09-1), which is sold by Givaudan under the trademark Parsol® 1789 and by Merck under the trade name Eusolex® 9020.

Advantageous further UVA filter substances include phenylene-1,4-bis-(2-benzimidazyl)-3,3,′5,5′-tetrasulfonic acid and its salts, particularly the corresponding sodium, potassium or triethanolammonium salts, in particular the phenylene-1,4-bis-(2-benzimidazyl)-3,3′,5,5′-tetrasulfonic acid bis-sodium salt with the INCI name Bisimidazylate, which is available, for example, under the trade name Neo Heliopan AP from Haarmann & Reimer.

Also advantageous are 1,4-di(2-oxo-10-sulfo-3-bornylidenemethyl)benzene and salts thereof (particularly the corresponding 10-sulfato compounds, in particular the corresponding sodium, potassium or triethanolammonium salt), which is also referred to as benzene-1,4-di(2-oxo-3-bornylidenemethyl-10-sulfonic acid).

Advantageous UV filter substances for the purposes of the present invention are also so-called broadband filters, i.e., filter substances which absorb both UVA and UVB radiation.

Advantageous broadband filters or UVB filter substances include, for example, bis-resorcinyltriazine derivatives. Particularly preferred are 2,4-bis{([4-(2-ethylhexyloxy)-2-hydroxylphenyl}-6-(4-methoxyphenyl)-1,3,5-triazine (INCI: Aniso Triazine), which is available under the trade name Tinosorb® S from CIBA-Chemikalien GmbH.

Particularly advantageous preparations for the purposes of the present invention that are characterized by a high or very high UVA protection preferably contain several UVA and/or broadband filters, in particular dibenzoylmethane derivatives [e.g., 4-(tert.butyl)-4′-methoxydibenzoylmethane], benzotriazole derivatives [e.g., 2,2′methylene-bis-(6-(2H-benzotriazol-2-yl)-4-(1,1,3,3-tetramethylbutyl)-phenol], phenylene-1,4-bis-(2-benzimidazyl)-3,3′,5,5′-tetrasulfonic acid and/or salts thereof, 1,4-di(2-oxo-10-sulfo-3-bornylidenemethyl)-benzene and/or salts thereof and/or 2,4-bis-{[4-(2-ethylhexyloxy)-2-hydroxy]-phenyl}-6-(4-methoxyphenyl)-1,3,5-triazine, individually or in any combinations with one another.

A further light protection filter substance which can be used advantageously according to the invention is ethylhexyl 2-cyano-3,3-diphenylacrylate (octocrylene), which is available from BASF under the designation Uvinul® N 539.

It may also be considerably advantageous to use polymer-bound or polymeric UV filter substances in preparations according to the present invention, in particular those described in WO-A-92/20690.

In addition, it may optionally be advantageous to incorporate further UVA and/or UVB filters into cosmetic or dermatological preparations according to the invention, for example, certain salicylic acid derivatives, such as 4-isopropylbenzyl salicylate, 2-ethylhexyl salicylate (-Octyl salicylate), and homomenthyl salicylate.

Of course, the list of cited UV filters which can be used for the purposes of the present invention is not intended to be limiting.

Preparations according to the invention advantageously contain substances which absorb UV radiation in the UVA and/or UVB range, in a total amount of, e.g., from 0.1% by weight to 30% by weight, preferably from 0.5% to 20% by weight, in particular from 1.0% to 15.0% by weight, based on the total weight of the preparations, in order to make available cosmetic preparations which protect the hair or the skin from the entire range of ultraviolet radiation. They can also be used as sunscreen compositions for the hair or the skin.

The cosmetic and dermatological preparations according to the invention may comprise cosmetic active agents, auxiliaries and additives, as are customarily used in such preparations, e.g., antioxidants, preservatives, bactericides, perfumes, antifoams, dyes, coloring pigments, thickeners, surfactants, emulsifiers, emollients, moisturizers and/or humectants, fats, oils, waxes and other customary constituents of a cosmetic or dermatological formulation, such as alcohols, polyols, polymers, foam stabilizers, electrolytes, organic solvents or silicone derivatives.

If the cosmetic or dermatological preparation according to the present invention is present in the form of a solution or emulsion or dispersion, the following may be used as solvents: water or aqueous solutions; oils such as triglycerides of capric or caprylic acid, preferably castor oil; fats, waxes and other natural and synthetic lipids, preferably esters of fatty acids with alcohols of low C number, for example with isopropanol, propylene glycol or glycerol, or esters of fatty alcohols with alkanoic acids of low C number or with fatty acids; alcohols, diols or polyols of low C number and their ethers, preferably ethanol, isopropanol, propylene glycol, glycerol, ethylene glycol, ethylene glycol monoethyl ether or monobutyl ether, propylene glycol monomethyl ether, monoethyl ether or monobutyl ether, diethylene glycol monomethyl ether or monoethyl ether, and analogous products.

In particular, mixtures of the above-mentioned solvents may be used. In the case of alcoholic solvents, water may be a further constituent.

The oil phase of the emulsions, oleogels or hydro- or lipodispersions in accordance with the present invention may advantageously be selected from esters of saturated and/or unsaturated, branched and/or unbranched alkanecarboxylic acids with a chain length of from 3 to 30° C. atoms and saturated and/or unsaturated, branched and/or unbranched alcohols with a chain length of from 3 to 30° C. atoms, from esters of aromatic carboxylic acids and saturated and/or unsaturated, branched and/or unbranched alcohols with a chain length of from 3 to 30° C. atoms. In this case, such ester oils may be selected advantageously from isopropyl myristate, isopropyl palmitate, isopropyl stearate, isopropyl oleate, n-butyl stearate, n-hexyl laurate, n-decyl oleate, isooctyl stearate, isononyl stearate, isononyl isononanoate, 2-ethylhexyl palmitate, 2-ethylhexyl laurate, 2-hexyldecyl stearate, 2-octyldodecyl palmitate, oleyl oleate, oleyl erucate, erucyl oleate, erucyl erucate, and synthetic, semisynthetic and natural mixtures of such esters, for example jojoba oil.

Furthermore, the oil phase may advantageously be selected from branched and unbranched hydrocarbons and hydrocarbon waxes, silicone oils, dialkyl ethers, saturated or unsaturated, branched or unbranched alcohols and fatty acid triglycerides, viz. the triglycerol esters of saturated and/or unsaturated, branched and/or unbranched alkanecarboxylic acids with a chain length of from 8 to 24, in particular from 12 to 18° C. atoms. For example, the fatty acid triglycerides may advantageously be selected from synthetic, semisynthetic and natural oils, for example olive oil, sunflower oil, soya oil, peanut oil, rapeseed oil, almond oil, palm oil, coconut oil, palm kernel oil and the like.

Any mixtures of such oil and wax components may also advantageously be employed in accordance with the present invention. If appropriate, it may also be advantageous to employ waxes, for example cetyl palmitate, as the only lipid component of the oil phase.

The oil phase may advantageously be selected from 2-ethylhexyl isostearate, octyldodecanol, isotridecyl isononanoate, isoeicosane, 2-ethylhexyl cocoate, C₁₂₋₁₅ alkyl benzoate, caprylic/capric acid triglyceride, dicaprylyl ether.

Especially advantageous mixtures are those of C₁₂₋₁₅ alkyl benzoate and 2-ethylhexyl isostearate, those of C₁₂₋₁₅ alkyl benzoate and isotridecyl isononanoate and those of C₁₂₋₁₅ alkyl benzoate, 2-ethylhexyl isostearate and isotridecyl isononanoate.

Amongst the hydrocarbons, liquid paraffin, squalane and squalene may advantageously be used according to the present invention.

The oil phase may furthermore advantageously comprise cyclic or linear silicone oils, or consist entirely of such oils, but it is preferred to use an additional content of other oil phase components, apart from the silicone oil(s).

Cyclomethicone (octamethylcyclotetrasiloxane) is advantageously employed as silicone oil to be used according to the invention. However, other silicone oils may also be used advantageously in accordance with the present invention, for example, hexamethylcyclotrisiloxane, polydimethylsiloxane, and poly(methylphenylsiloxane).

Especially advantageous mixtures are furthermore those of cyclomethicone and isotridecyl isononanoate and of cyclomethicone and 2-ethylhexyl isostearate.

If appropriate, the aqueous phase of the preparations according to the invention may advantageously comprise alcohols, diols or polyols of low C number, and their ethers, preferably ethanol, isopropanol, propylene glycol, glycerol, ethylene glycol, ethylene glycol monoethyl ether or monobutyl ether, propylene glycol monomethyl ether, monoethyl ether or monobutyl ether, diethylene glycol monomethyl ether or monoethyl ether and analogous products, furthermore alcohols of low C number, for example ethanol, isopropanol, 1,2-propanediol, glycerol, and, in particular, one or more thickeners which may advantageously be selected from silicon dioxide, aluminum silicates, polysaccharides and their derivatives, for example hyaluronic acid, xanthan gum, hydroxypropyl methylcellulose, especially advantageously from polyacrylates, preferably a polyacrylate from the group of the so-called Carbopols, for example type 980, 981, 1382, 2984 and 5984 Carbopols, in each case individually or in combination.

Gels which may be used according to the present invention usually comprise alcohols of low C number, for example ethanol, isopropanol, 1,2-propanediol, glycerol and water, or an above-mentioned oil in the presence of a thickener, which is preferably silicon dioxide or an aluminum silicate in the case of oily-alcoholic gels, and preferably a polyacrylate in the case of aqueous-alcoholic or alcoholic gels.

Solid sticks may comprise, for example, natural or synthetic waxes, fatty alcohols or fatty acid esters.

Customary basic materials which are suitable for use as cosmetic sticks in accordance with the present invention include liquid oils (for example liquid paraffin, castor oil, isopropyl myristate), semi-solid constituents (for example petrolatum, lanolin), solid constituents (for example beeswax, ceresine and micro-crystalline waxes, or ozocerite) and waxes of high melting point (for example carnauba wax and candelilla wax).

Suitable propellants for cosmetic and/or dermatological preparations in accordance with the present invention which can be sprayed from aerosol containers are the customary known volatile, liquefied propellants, for example hydrocarbons (propane, butane, isobutane), which may be employed individually or as a mixture with each other. Pressurized air may also be used advantageously.

Those of skill in the art will, of course, be familiar with the fact that there are non-toxic propellants, which would be suitable in principle for putting into practice the present invention in the form of aerosol preparations; however, it is recommended to dispense with the use of these-in particular fluorohydrocarbons and fluorochlorohydrocarbons (FCHCs)-due to their unacceptable effect on the environment or other accompanying circumstances.

Cosmetic preparations in accordance with the present invention may also take the form of gels which comprise not only an effective amount of active ingredient according to the invention and conventionally used solvents therefor, preferably water, but also organic thickeners, for example gum arabic, xanthan gum, sodium alginate, cellulose derivatives, preferably methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropyl methylcellulose, or inorganic thickeners, for example, aluminum silicates such as, for example, bentonites, or a mixture of polyethylene glycol and polyethylene glycol stearate or polyethylene glycol distearate. The gel comprises the thickener for example in an amount of between 0.1 and 30% by weight, preferably between 0.5 and 15% by weight.

It is particularly advantageous for the purposes of the present invention if the cosmetic or dermatological preparations according to the present invention contain further active substances, in particular natural active substances and/or derivatives thereof, such as, e.g., alpha-lipoic acid, phytoene, D-biotin, coenzyme Q10, alpha-glucosyl rutin, carnitine, carnosine, osmolytes, clover extract, hop extract or hop-malt extract.

The concentration of the active ingredients (one or more substances) is advantageously from 0.0001% to 30% by weight, based on the total weight of the preparations. The cosmetic or dermatological preparation according to the present invention may be prepared by any method known within the art.

Treatment of Organs and Tissue Samples

Perfusing organs or tissue samples with a composition comprising an anti-freeze polypeptide according to the present invention makes it possible the store such organs and tissue samples, or other biological materials, at a lower temperature, thereby preventing deterioration or degradation of the sample, but without the risk of generating a freeze damage to said tissues, organs, cells or other biological materials. In many instances, damage to organs and biological tissues is caused not so much by the generation of a frozen state of the organ or tissue in question, but by the recrystallization which may occur.

Examples of biological materials, organs and tissue samples include, but is not limited to, e.g. samples comprising one or more polypeptides, samples comprising one or more microsomes or micelles comprising one or more polypeptides, samples comprising whole blood, samples comprising blood plasma, samples comprising blood platelets, samples comprising red blood cells, samples comprising semen, samples comprising gametes.

Tissue culture samples can comprise any form of biological cells, including mammalian cells, such as animal cells and human cells, rodent cells and insect cells. The organ to be treated can be e.g. a kidney, a lung, a heart, a spleen or a liver. Accordingly, there is provided a method for inhibiting recrystallization of an organ or a biological sample, said method comprising the step of contacting the organ or biological sample with the polypeptide according to the present invention under conditions allowing the polypeptide to prevent recrystallization of the organ or the biological sample.

There is also provided a method for improving the preservation of an organ or a biological sample, said method comprising the step of contacting the organ or biological sample with the polypeptide according to the present invention under conditions allowing the polypeptide to contact the organ or biological sample in question, thereby allowing the organ or biological sample to be stored stored at a sub-freezing temperature as compared to the storage temperature of an untreated organ or biological sample.

A new method has recently been developed which is based on vitrification. This method suffers, however, from the fact that during de-freezing of the gameter, embryos or stem cells relaxation occurs, which means that ice crystals are formed. During the de-freezing process these ice crystals grow due to re-crystallisation. It is believed that if one or more of the polypeptides according to the present invention is present in the solvents in which the gameter, embryos or stem cells are present during this vitrification process, then the crystal formation as well as the crystal growth will be markedly reduced or may be even prevented.

Further cryo-protective uses of the polypeptides according to the present invention are disclosed in more detail in the following.

In a separate aspect the present invention relates to methods for protecting cells and their membranes from damage which they would otherwise suffer upon exposure to non-physiological conditions such as temperature abnormalities, including both hyperthermic, hypothermic and subfreezing temperatures. Improved rates of cell viability are observed over a wide range of conditions which do not involve ice formation, including temperatures above the freezing range as well as temperatures below the freezing range but in vitrification conditions. Heretofore the only known property of these polypeptides was their ability to interact with ice crystals. In conditions in which ice crystals are formed, it is further discovered that use of the polypeptides with human cells at the concentrations in which they naturally occur in the source organisms results in aggravating the injury to the cells rather than reducing it, but that the injury is lessened, and the survival rate improved, by using low concentrations. The polypeptides thus offer benefits in the preservation and improved viability of cell suspensions, tissues and whole organs. The polypeptides are further discovered to have the ability to block ion channels in mammalian cell membranes, thereby providing a further utility in the treatment of disease conditions.

The present invention makes use of the recognized but unutilized quality of anti-freeze polypeptides and their ability to interact with cells and cell membranes. The interaction occurs with cell membranes in a wide range of structures, including individual cells in cell suspensions, connected cell masses in tissues and organs, and cell structures which are pervaded with a vascular system. The interaction is a favorable one, imparting to the cell membranes and the structures which incorporate these membranes a variety of benefits, including improvements in cell viability and survival rate, prolongation of the functionality, stability and structural integrity of the cells and cell tissues, reduction of the occurrence of structural damage to membranes and cells under adverse conditions, and control of the transport of ions across the cell membranes.

The various types of interaction are unrelated to the known effects of these polypeptides on ice crystal propagation, since the beneficial effects of these interactions are observed under conditions where ice crystals do not form at all, in addition to their occurrence in the presence of ice crystals. For example, benefits are observed at temperatures ranging from cryogenic temperatures to temperatures well above physiological temperatures. The invention thus extends to situations involving physiological conditions as well as nonphysiological conditions, and to situations that involve the presence of ice crystals as well as those in which ice crystals are completely absent. Nonphysiological conditions in which beneficial effects on viable cells and cell membranes are observed therefore include: (i) hypothermal conditions defined by temperatures above the normal freezing point of water (0° C.), and therefore with no possibility of ice formation, and below the physiological temperature of the cells; (ii) vitrification conditions defined by temperatures at or below the glass formation (or glass transition) temperature, such as for example from 150K down to about 4K, and by the presence of vitrifying agents which promote vitrification and inhibit crystallization; (iii) freezing conditions, such as temperatures from the normal freezing point of water down to about 4K, which permit the formation of ice crystals; (iv) hyperthermal conditions defined by temperatures above the physiological temperature of the cells, for example temperatures within the range of the physiological temperature up to about 10° C. above the physiological temperature; and (v) conditions defined by chemical environments which differ from the physiological chemical environment of the cells, such as conditions of nonphysiological pH and other variations from the physiological chemical composition, as well as such conditions in combination with conditions of nonphysiological temperature.

Applicability of the invention aim extends to abnormal physiological conditions such as diseases associated with the instability of cell membranes and diseases associated with imbalances of ions between intracellular and extracellular spaces giving rise to abnormal ion transport across the cell membranes. The unexpected nature of this behavior is heightened by the discovery that the blockage of ion channels, such as for example those of calcium and potassium ion in epithelial cells, is achieved without interference with other metabolic functions of the cells, including ATP ion pumps and interactions with carbachol. Still further, the invention offers benefits to cells in normal physiological conditions, such as through the use of cosmetics or medications designed to restore, preserve or repair epidermal tissue.

The invention finds applicability to a wide range of living cells, including both animal cells and plant cells. A particularly unusual and interesting discovery in connection with the present invention, however, is the utility of the anti-freeze polypeptides in the treatment and preservation of mammalian cells, tissues and organs. In their natural form, these polypeptides exist in non-mammalian species only, and the differences in cell and membrane structure as well as in blood and cytoplasm composition between these species and mammalian species renders the presently discovered benefits surprising and unexpected. The invention is thus of particular interest and utility as applied to mammalian cells, tissues, organs and organisms which are exposed to conditions which differ from the normal physiological condition of the mammal. Examples of cells to which the invention is applicable are mammalian oocytes, hepatocytes, erythrocytes and leukocytes, and various types of plant cells. Examples of tissues and organs are tissue of livers, hearts, and kidneys, and the organs themselves. Examples of organisms are embryos, self-sustaining whole animals, plant seeds and whole plants.

Additional benefits arising from the invention are many and varied. Included among these are the elimination of the need to maintain a fast cooling rate during freezing to cryogenic temperatures, the ability of the polypeptides to raise the viscosity of solutions at considerably lower concentrations than known cryoprotectants, and the ability of the polypeptides to preserve foods upon freezing. Other advantages, benefits, and applications of the present invention will be apparent from the description which follows.

Within the technical field of cryopreservation, the following terms are used with the following definitions:

“Abnormal” or “non-physiological conditions” for cells, tissues, organs or organisms refer to conditions which differ from the normal physiological conditions. Abnormal or nonphysiological conditions include, but are not limited to, a temperature which is significantly higher or lower than the normal physiological temperature of the healthy organism of which the cell, tissue or organ is native, or the organism itself; an excess or subnormal amount of carbon dioxide, oxygen, inorganic salts, or organic compounds, a pH value significantly higher or lower than that of the healthy organism, and combinations of these conditions.

“Anti-freeze polypeptides,” “anti-freeze polypeptides” (“AFPs”), “anti-freeze glycoproteins” and “anti-freeze glycopeptides” (“AFGPs”) refer to macromolecules found in the body fluids of some animals, which have the commonly known property that they reduce non-colligatively the freezing point of water. Anti-freeze polypeptides, polypeptides, glycoproteins and glycopeptides are also known as “thermal hysteresis polypeptides” because the temperature at which freezing occurs is depressed to a greater degree than one could attribute to any colligative character of the polypeptides, whereas the temperature at which ice melts during melting is depressed is significantly less, in accordance solely with colligative behavior.

“Cryogenic temperatures” refers to temperatures below 0° C.

“Freezing” refers to the transition from the liquid phase of water to the solid phase of water.

“Hyperthermic” refers to temperatures higher than the normal physiological temperature of a cell, tissue, organ or organism, such as for example from slightly above the physiological temperature up to about 20° C. above, preferably to about 10° C. above, and more preferably to about 5° C. above the physiological temperature.

“Hypothermic” refers to temperatures lower than the normal physiological temperature of a cell, tissue, organ or organism, but not low enough to cause a phase transition to the solid phase.

“Isolated and purified” refers to molecular species which are extracted from the organism in which they naturally occur, and concentrated by conventional laboratory techniques such as chromatography, preferably to a concentration of at least about 85%, more preferably to at least about 95%. This invention further extends to molecules which have a molecular structure which is either the same, highly similar, or homologous to naturally occurring forms of the molecules, and which may have been synthesized either by chemical means or by recombinant DNA techniques.

“Mammal” refers to any warm blooded mammal as the term is generally used in biology, including, for example, pig, cow, rabbit, horse and human being.

“Polar fish species” refers to cold-blooded aquatic animals, particularly vertebrates, which reside in waters of the polar regions of the earth, including the regions within the Arctic and Antarctic Circles. Polar fish species of particular interest in connection with this invention are those which remain in waters which become or remain ice-laden.

“Spicule” and “spicular” refer to ice crystals and ice crystal growth in which the dominant direction of crystal propagation is along the c-axis, i.e., perpendicular to the basal plane, to form crystals having a needle-like shape.

“Viable” means capable of living, capable of surviving and developing under, or upon a return to, normal physiological conditions, or capable of germinating under conditions normally favorable to germination.

“Vitrification” refers to solidification at cryogenic temperatures in such a manner that a glass phase, i.e., a non-crystalline solid, is formed, as opposed to crystalline ice. “Apparent vitrification” refers to vitrification as determined by visual observation under a microscope. Vitrification of a biological material is generally achieved by introducing any of a variety of cryoprotective or “vitrifying” agents, including polyhydric alcohols such as glycerol and propylene glycol, or other compounds such as dimethylsulfoxide into the material. The introduction of vitrifying agents is often accompanied by relatively high rates of cooling. The optimal rates in each case vary with the composition and thermodynamics of the system. Typical cooling rates in most cases for small unorganized cells such as ova, sperm, and embryos, and for organs, generally fall within the ranges of about 100° C./min to about 2,000° C./min, preferably about 200° C./min to about 1,750° C./min, and more preferably about 700° C./min to about 1,750° C./min. Rates on the order of 1500° C./min are commonly used.

In the practice of the present invention, the anti-freeze polypeptides according to the present invention are generally used in the form of a liquid solution, and preferably an aqueous solution. The anti-freeze polypeptides according to the present invention may be used individually or in combination with other polypeptides. When the polypeptides are used in combination, it will often be most convenient to use the polypeptides in the physiological combinations in which they naturally occur in the source species, i.e., the same mixture and proportions of the polypeptide species as they are found in the fluid of the fish, insect or other organism from which they are extracted, although isolated from other components of the fluid and redissolved in a different solvent or solution, perhaps at a total concentration which differs from that in which the mixture is present in its natural environment. In certain cases, however, activity and effectiveness may be improved by fractionating the polypeptides in the source mixture and selecting and recombining fractions in an optimal manner.

The concentration of the anti-freeze polypeptides according to the represent invention in the liquid solution as used in the present invention may vary widely, although in certain cases, improved results will be obtained within certain concentration ranges, and in certain cases, the concentration must be restricted to certain ranges to avoid injury caused by the polypeptides themselves. In general, however, the polypeptides will be used in concentrations of from about 0.01 mg/mL to about 80 mg/mL, preferably from about 0.1 mg/mL to about 60 mg/mL, more preferably from about 1 mg/mL to about 40 mg/mL, and most preferably from about 1 mg/mL to about 20 mg/mL. When used with human cells, particularly under temperatures below the physiological temperature of the cells, preferred concentrations are from about 0.1 mg/mL to about 40 mg/mL, more preferably from about 0.1 mg/mL to about 3 mg/mL. In applications where the polypeptides are used to protect tissue at temperatures below the physiological temperature of the tissue, preferred concentrations are within the range of about 0.1 mg/mL to about 50 mg/mL, and when the tissue is human tissue, preferred concentrations are within the range of about 0.1 mg/mL to about 3 mg/mL. In applications where the polypeptides are used to protect cells in general at temperatures below the physiological temperature of the cells but above the freezing temperature of the cells, or below the freezing temperature of the cells but in the presence of a vitrifying agent or other non-peptide cryoprotectant, preferred concentrations are within the range of about 0.01 mg/mL to about 60 mg/mL, and more preferred concentrations are within the range of about 1 mg/mL to about 40 mg/mL. In applications where the polypeptides are used to block ion channels across cell membranes, preferred concentrations are at least about 0.01 mg/mL, more preferably at least about 0.1 mg/mL, and most preferably from about 0.5 mg/mL to about 40 mg/mL. All concentrations of anti-freeze polypeptides are expressed as totals of the concentrations of individual anti-freeze polypeptides when a solution contains a mixture of different anti-freeze polypeptides.

Aqueous solutions of the anti-freeze polypeptides for use in the present invention may further contain any of the wide variety of mixtures of salts, sugars, ions and other nutrients which are included in electrolyte solutions known in the art to be useful for preserving biological agents. These include tissue culture media, organ perfusion fluids, and the like. Electrolyte solutions are particularly useful for enhancing the biological compatibility of the polypeptides. Examples of the many electrolyte solutions known in the art are: Physiological Saline, in which the NaCl concentration is either 0.9% or 0.95% Ringer's Injection Solution (U.S.), listed in Facts and Comparisons, p. 50, Lippincott Publishing Co., St. Louis, Mo. (October 1981) Mammalian Ringer's Solution (U.K. and Canada), listed by Best and Taylor, Basis of Medical Practice, 6th ed., Baltimore (1950) Lactated Ringer's Solution (U.S.), listed in Facts and Comparisons, p. 50, Lippincott Publishing Co., St. Louis, Mo. (October 1981) Lactated Ringer's Solution (Hartmann), listed by Hartmann, A. F., J. Am. Med. Assoc. 103:1349-1354 (1934) Acetated Ringer's Solution, listed by Fox, C. L., et al., J. Am. Med. Assoc. 148:825-833 (1952) Locke's Solution, listed by Locke, F. S., Zbl. Physiol. 8:166 (1894); 14:670 (1900); 15:490 (1901) Tyrode's Solution, listed by Tyrode, M. J., Arch. Int. Pharmacodyn. 20:205 (1910) Krebs Henseleit Solution, listed by Krebs, H. A., et al., Hoppe-Seyle's Z. Physiol. Chem. 210:33-66 (1932) Krebs Ringer Phosphate Solution, listed by Krebs, H. A., Hoppe-Seyle's Z. Physiol. Chem. 217:193 (1933) Krebs Serum Substitute Solution, listed by Krebs, H. A., Biochem. Biophys. Acta 4:249-269 (1950) Krebs Improved Ringer II Solution, listed by Krebs, H. A., Biochem. Biophys. Acta 4:249-269 (1950) Krebs Improved Ringer III Solution, listed by Krebs, H. A., Biochem. Biophys. Acta 4:249-269 (1950) Krebs Liver Perfusion Solution with Bovine Serum Albumin and Red Cells, listed by Hem, R., et al., Biochem. J. 101:284 (1966) Schimassek Liver Perfusion Solution, listed by Schimassek, H., et al., Biochem. Z. 336,440 (1963) Krebs Kidney Perfusion Solution, listed by Nishiitsutsuji-Uwo, J., et al., Biochem. J. 103:852-862 (1967) Hepatocyte Incubation Solution, listed by Crow, K. E., et al., Biochem. J. 172:29-36 (1978) Bahlman Kidney Perfusion Solution, listed by Bahlman, J., et al., Am. J. Physiol. 212:77 (1967) Fulgraff Kidney Perfusion Solution, listed by Fulgraff, et al., Arch. Int. Pharmacodyn. 172:49 (1972)

The optimal choice of electrolyte solution for any particular application will vary with the application, such as, for example, the form of the cells (whether the cells are present as cell suspensions, tissues, or organs) to be treated or protected by the anti-freeze polypeptides, the animal from which the cells are derived, and the conditions to which the cells have been, or are expected to be, exposed.

In embodiments of the invention involving vitrification conditions, the anti-freeze polypeptides are used in combination with vitrifying agents which prevent or inhibit ice crystal formation during solidification of the intracellular and extracellular fluids upon cooling to sub-freezing temperatures. Various vitrifying agents are known in the art, and may be used either individually or in combination with other vitrifying agents or biologically compatible solutes. Examples of vitrifying agents are glycerol, dimethyl sulfoxide, ethylene glycol, polyvinylpyrrolidone, glucose, sucrose, propanediol, butanediol, and carboxymethyl cellulose. Polyhydric alcohols as a class are useful as vitrifying agents. Prominent examples are glycerol, ethylene glycol, propanediol, butanediol, and butanetriol. Concentrations of vitrifying agents may vary widely, depending on the concentrations of other components in the system, the cooling rate and the lowest temperature reached. In general, best results will be obtained with concentrations of from about 5% to about 35% by weight. Vitrification is usually practiced with a rapid cooling rate, such as for example a rate exceeding 100° C./min, and preferably exceeding 1,000° C./min.

In embodiments which involve the use of non-peptide cryoprotectants, without necessarily avoiding the formation of ice crystals, many of the same considerations apply. The agents listed above as examples of vitrifying agents serve as well as cryoprotectants, within similar concentration ranges.

The beneficial effect of the anti-freeze polypeptides on cells and/or cell membranes is achieved by placing the polypeptides in contact with the cells and maintaining such contact throughout, or for a substantial portion of, the period of exposure to otherwise injurious conditions. When the cells are in the form of cell suspensions, contact of this type is achieved by simply adding the polypeptides to the suspension fluid. When the cells are in the form of tissues or organs, contact is achieved by immersing the tissues or organs in a solution of the polypeptides. When the cells are in the form of tissues or organs which contain a vascular system, contact is achieved by perfusing the vascular system with a solution of the polypeptides, and once perfused, holding the polypeptide solution in the vascular system throughout the period of storage, preservation or exposure to the injurious conditions. Methods of perfusion are well known among those skilled in physiology and surgical procedures.

Cells which can benefit from treatment with the anti-freeze polypeptides in accordance with this invention include cells of a wide variety of types. Examples are oocytes, embryos, leukocytes, erythrocytes, platelets, pancreatic islets, and hepatocytes. Organs which can benefit from the present invention are also widely varied. Examples include the liver, kidney, heart, brain, lung, pancreas, spleen, ovary, and stomach. Tissues which can benefit from the invention include tissues of any of these organs, as well as skin tissue, bone marrow tissue, cornea tissue, and a wide range of others. The invention finds applicability to mammals in general, and will be of particular interest and utility when used in connection with human cells, tissues and organs.

The effect of the anti-freeze polypeptides according to the present invention in inhibiting ion transport across cell membranes extends to a variety of ions, with particular interest to Ca⁺⁺, K⁺ and Na⁺ ions, as well as two or more of these ions in combination.

Since excessive ion transport is one physiological effect which accompanies hypothermia, the ability of the anti-freeze polypeptides according to the present invention to inhibit ion transport may be related to the ability of the polypeptides to enhance cell viability under hypothermic conditions. Accordingly, the amounts and concentrations of polypeptide administered to achieve the effect of inhibition of ion transport are generally the same or similar to the amounts used in enhancing viability under hypothermic exposure.

The ability of the polypeptides to inhibit ion transport across cell membranes also renders the polypeptides useful in treating diseases and abnormal physiological conditions in which excessive trans-membrane ion transport is present. Examples of such diseases and conditions are cystic fibrosis, Kartagener's Syndrome, diabetes insipidus, diabetes mellitus, and antidiuretic hormone abnormalities. Administration of the polypeptides for this effect may be achieved by ingestion, vascular injection, localized application, and various means in general by which other drugs or treatment agents are administered when used in the treatment or management of these diseases and conditions. Again, the concentrations for useful results are generally the same as those referred to above, and the dosage or frequency of administration will be determined by the degree to which the condition being treated has progressed as well as the observed response to the treatment.

Application of the anti-freeze polypeptides comprising the polypeptide of the present invention also extend to the use of the polypeptides in the preservation of foods which have a cellular structure. Foods of particular interest for this application are meats and meat products, but other types of foods will benefit as well. For purposes of this invention, meats and meat products include fresh meat and poultry, as well as frozen, canned and dried meats and poultry. Many such foods when cooled to avoid spoilage during transport or storage tend to lose turgor, freshness and other qualities which contribute to their taste, mouthfeel and general appeal. These qualities can be preserved by treatment of the foods with solutions of the polypeptides in accordance with the present invention. The mode of treatment will vary from one type of food to the next, but will generally involve equilibration of the food with the polypeptide in solution, either by immersion, perfusion, or any other kind of absorption or other means of achieving prolonged contact. The types of solutions and the methods of immersion and perfusion described above in connection with other applications of the invention will be applicable here as well.

Fluids Comprising Polypeptides According to the Present Invention

The use of the anti-freeze polypeptides according to the present invention as an additive e.g. to fluids and liquids, such as refridgerants and many different types of aqueous solutions is provided in accordance with the present invention in order to prevent freezing of the refridgerant or the aqueous solutions. The feature of preventing freezing of a solution is beneficial in many different technical areas.

Carriers and Solid Supports Linked to the Polypeptides According to the Present Invention

A polypeptide according to the present invention can be linked to a carrier, such as a solid support or a semi-solid support. The polypeptide can be covalently or non-covalently linked to any such carrier, for example a surface of a material desirably displaying the polypeptides according to the invention. Surfaces and solid supports according to the invention can comprising one or more polypeptides according to the invention, or functional fragments thereof displaying anti-freeze activity, which are either directly or indirectly attached to the surface, such as a solid or semi-solid support.

Attachment includes in principle all state-of-the-art technologies for covalently or non-covalently attaching a polypeptides to a surface e.g. directly, through a linker residue, through entrapment of the polypeptides in a caged structure, which retains the polypeptides in reactive contact with the surface, or any other way of attaching the polypeptide(s) according to the present invention to a solid support or semi-solid support.

A number of techniques are available for attaching polypeptides to solid or semi-solid supports. The attachment of a polypeptide to a solid surface has e.g. been disclosed by Cordek et al. 1999, Anal. Chem., 71: 1529-1533; Blasi et al. 2005, Enzyme and Microbial Tech., 36: 818-823; Parrado et al. (1995), Proc. Chem., 30(8): 735-741; Yakovleva et al. (2003), Biosensors and Bioelectronics, 19: 21-34; Cao, L., Carrier-bound Immobilized Enzymes. Principles, Applications and Design, Wiley-VCH, 2005; and Immobilization of Enzymes and Cells (Methods in Biotechnology), Birkerstaff, G. F., eds., Humana Press, 1997. Other techniques are also available and well known to the skilled person.

In one aspect of the present invention there is provided a coating composition comprising one or more polypeptides according to the present invention. The coating composition can further comprise one or more further ingredients, including pigments and resins, as disclosed herein below in more detail.

Much attention has been focused on immobilization of biomolecules, including polypeptides, in silicate glass formed by the sol-gel method (Eggers et al., Protein Sci. 2001, 10, 250-261). The process involves hydrolyzing an alkoxide to produce a sol, which then undergoes polycondensation to form a gel. Biomolecules are immobilized by being entrapped in the gel during the sol-to-gel transition. The sol-gel materials offer advantages over more traditional organic polymers for biomolecule entrapment in that these materials have increased mechanical strength, chemical stability, biocompatibility, and resistance to microbial attack.

The sol-gel encapsulation of polypeptides according to the present invention can be performed by using precursors, based around polyol silicates and polyol siloxanes, especially those derived from glycerol. Poly(glyceryl silicate) (PGS) can be prepared and employed for sol-gel bioentrapment of the polypeptides, in an approach distinguished by a high degree of biocompatibility and mild encapsulation conditions, and which enables the reproducible and efficient confinement of the polypeptides inside silica.

The above-disclosed methodology can be extended to metallosilicate, alkylsiloxane, functionalized siloxane, and various composite sol-gels, thereby allowing the fabrication of a physicochemically diverse range of bio-doped polymers comprising the anti-freeze polypeptides according to the present invention.

The hybrid materials according to the present invention preferably display activities approaching those of the free anti-freeze polypeptides, together with high stabilities and robustness that characterize sol-gel bioceramics.

In one aspect of the present invention, a sol-gel process well know to those of ordinary skill in the art is used for attaching the polypeptides according to the invention to the solid or semi-solid support. The sol-gel process is conventional and typically produces a sol-gel glass, which results from an optically transparent amorphous silica or silicate material produced by forming interconnections in a network of colloidal sub-micrometer particles under increasing viscosity until the network becomes completely rigid, with about one-half the density of glass. Accordingly, a sol-gel glass comprising one or more polypeptides according to the invention is also claimed. Reference is made—among others—to Gill and Ballesteros, J. Am. Chem. Soc. 1998, 120, 8587-8598. Solution polymerization to form sol of cross-linked particles is disclosed e.g. in U.S. Pat. No. 5,863,996 and can be used in conjunction with the present invention.

The coating compositions according to another aspect of the present invention preferably comprises a resin which is compatible with the polypeptide(s) according to the present invention—i.e. allows said polypeptides to exert an anti-freeze activity when forming part of the coating composition. Resins are well-known in the art and polypeptide compatible resins are also disclosed in the prior art. See e.g. WO 01/72911 and U.S. Pat. No. 5,998,200.

There is also provided in accordance with the present invention a composition comprising a mesoporous aerogel having a three-dimensional nanoarchitecture comprising the polypeptides according to the present invention. The three-dimensional nanoarchitecture preferably comprises a colloidal metal encapsulating anti-freeze polypeptide bio-composite superstructure which is nano-glued therein. Accordingly, there is also provided a method for making a mesoporous aerogel having a three-dimensional nanostructure with a colloidal metal encapsulating anti-freeze polypeptide biocomposite nanoglued therein, said method comprising the steps of: Forming a metal encapsulating anti-freeze polypeptide biocomposite by mixing together said one or more anti-freeze polypeptides according to the present invention and said colloidal metal; forming a sol, such as a silica sol, by mixing together a catalyst and an alkoxide, such as a silicon alkoxide; forming a gel by mixing together said sol, such as a silica sol, and said biocomposite and allowing said sol to gel; and extracting and supercritically drying said gel with carbon dioxide to form said aerogel with said metal encapsulating anti-freeze polypeptide bio-composite superstructure nanoglued therein. In one embodiment, the mesoporous aerogel is a silica mesoporous aerogel. Reference is made to US 2004/0209338.

Use of the Polypeptides According to the Present Invention in the Inhibition of the Formation of Gas Hydrates.

The polypeptides according to the present invention may also find use in the inhibition of the formation of gas hydrates in the oceans. It is well known that gas hydrates are ice-like crystalline molecular complexes formed from mixtures of water and suitably sized “guest” gas molecules. The water (host) molecules, upon hydrogen bonding, form lattice structures with several interstitial cavities. The guest gas molecules can occupy the lattice cavities, and when a minimum number of cavities are filled, the crystalline structure will become stable and solid gas hydrates will form, even at temperatures well above the melting point of water ice. When gas hydrates dissociate (melt), the crystalline lattice breaks down into liquid water (or converts to ice if conditions are below the freezing point of water) and the gas is released. Commercially, the gas may be utilized for energy production. However, the phenomenon does represent an environmental risk in cases where the gas escapes in a non-controlled manner. This could be in areas where earth crakes appear from time to time.

It is well known that the problem with regard to gas hydrate formation also occurs in pipeline on the bottom of the oceans. However, due to the ice crystal formation inhibiting properties of the polypeptides according to the present invention, it is believed that the presence of said polypeptides may prevent the formation of gas hydrates, due to the fact that the structure of these hydrates are very similar to the structure of ice crystals.

Selected items of the present invention are disclosed herein below.

-   -   1. A polypeptide comprising a plurality of consecutively linked         amino acid residues, said polypeptide comprising the sequence:

X₁-X₂-X₃-X₄-X₅-X₆-X₇-X₈-X₉, (SEQ ID NO: 90)

-   -   -   wherein         -   X₁ is selected from the group of amino acid residues             consisting of S, A, G and D;         -   X₂ is selected from the group of amino acid residues             consisting of A, V, I, T and S;         -   X₃ is selected from the group of amino acid residues             consisting of non-bulky amino acid residues;         -   X₄ is selected from the group of amino acid residues             consisting of S, I, T and V;         -   X₅ is selected from the group of amino acid residues             consisting of S, A, I and T;         -   X₆ is selected from the group of amino acid residues             consisting of S, T and V;         -   X₇ is selected from the group of amino acid residues             consisting of non-bulky amino acid residues;         -   X₈ is selected from the group of amino acid residues             consisting of S, T and V;         -   X₉ is selected from the group of amino acid residues             consisting of S, A and G;         -   wherein at least one of the residues X₂, X₄, X₆ and X₈ of             SEQ ID NO:90 is T or

V;

-   -   -   wherein the total number of amino acid residues of the             polypeptide is less than 250; and         -   wherein the polypeptide possesses an ice-binding capability.

    -   2. The polypeptide according to item 1, wherein X₁ is S.

    -   3. The polypeptide according to item 1, wherein X₁ is A.

    -   4. The polypeptide according to item 1, wherein X₁ is G.

    -   5. The polypeptide according to item 1, wherein X₁ is D.

    -   6. The polypeptide according to item 1, wherein X₂ is A.

    -   7. The polypeptide according to item 1, wherein X₂ is V.

    -   8. The polypeptide according to item 1, wherein X₂ is I.

    -   9. The polypeptide according to item 1, wherein X₂ is T.

    -   10. The polypeptide according to item 1, wherein X₂ is S.

    -   11. The polypeptide according to item 1, wherein X₃ does not         contain a cyclic aliphatic side chain or an aromatic side chain.

    -   12. The polypeptide according to item 1, wherein X₄ is S.

    -   13. The polypeptide according to item 1, wherein X₄ is I.

    -   14. The polypeptide according to item 1, wherein X₄ is T.

    -   15. The polypeptide according to item 1, wherein X₄ is V.

    -   16. The polypeptide according to item 1, wherein X₅ is S.

    -   17. The polypeptide according to item 1, wherein X₅ A.

    -   18. The polypeptide according to item 1, wherein X₅ I.

    -   19. The polypeptide according to item 1, wherein X₅ T.

    -   20. The polypeptide according to item 1, wherein X₆ is S

    -   21. The polypeptide according to item 1, wherein X₆ is T.

    -   22. The polypeptide according to item 1, wherein X₆ is V.

    -   23. The polypeptide according to item 1, wherein X₇ does not         contain a cyclic aliphatic side chain or an aromatic side chain.

    -   24. The polypeptide according to item 1, wherein X₈ is S.

    -   25. The polypeptide according to item 1, wherein X₈ is T.

    -   26. The polypeptide according to item 1, wherein X₈ is V.

    -   27. The polypeptide according to item 1, wherein X₉ is S.

    -   28. The polypeptide according to item 1, wherein X₉ is A.

    -   29. The polypeptide according to item 1, wherein X₉ is G.

    -   30. The polypeptide according to item 1, wherein at least one of         the residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 is T.

    -   31. The polypeptide according to item 1, wherein at least two of         the residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 are T.

    -   32. The polypeptide according to item 1, wherein at least three         of the residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 are T.

    -   33. The polypeptide according to item 1, wherein all four of the         residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 are T.

    -   34. The polypeptide according to item 1, wherein at least one of         the residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 is V.

    -   35. The polypeptide according to item 1, wherein at least two of         the residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 are V.

    -   36. The polypeptide according to item 1, wherein at least three         of the residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 are V.

    -   37. The polypeptide according to item 1, wherein all four of the         residues X₂, X₄, X₆ and X₈ of SEQ ID NO:90 are V.

    -   38. The polypeptide according to item 1, wherein the maximum         number of amino acid residues of the polypeptide is less than         240, such as less than 230, for example less than 220, such as         less than 210, for example less than 200, such as less than 190,         for example less than 180, such as less than 150, for example         less than 140, such as less than 130, for example less than 120,         such as less than 110, for example less than 100, such as less         than 95, for example less than 90, such as less than 85, for         example less than 80, such as less than 75, for example less         than 70, such as less than 65, for example less than 60, such as         less than 55, for example less than 50, such as less than 45,         for example less than 40, such as less than 30, for example less         than 20, such as less than 15.

    -   39. The polypeptide according to item 1, wherein the minimum         number of amino acid residues of the polypeptide is 10 or more,         such as 12 or more, for example 14 or more, such as 16 or more,         for example 18 or more, such as 20 or more, for example 22 or         more, such as 24 or more, for example 26 or more, such as 28 or         more, for example 30 or more, such as 32 or more, for example 34         or more, such as 36 or more, for example 38 or more, such as 40         or more, for example 42 or more, such as 44 or more, for example         46 or more, such as 48 or more, for example 50 or more, such as         55 or more, for example 60 or more, such as 65 or more, for         example 70 or more, such as 75 or more, for example 80 or more,         such as 85 or more, for example 90 or more, such as 95 or more,         for example 100 or more.

    -   40. The polypeptide according to item 1 further comprising a         second copy of SEQ

ID NO:90, which does not overlap with the first copy of SEQ ID NO:90, wherein the second copy of SEQ ID NO:90 comprises the sequence:

X_(a)-X_(b)-X_(c)-X_(d)-X_(e)-X_(f)-X_(g)-X_(h)-X_(i), (SEQ ID NO: 90)

-   -   -   wherein         -   X_(a) is selected from the group of amino acid residues             consisting of S, A, G and D;         -   X_(b) is selected from the group of amino acid residues             consisting of A, V, I, T and S;         -   X_(c) is selected from the group of amino acid residues             consisting of non-bulky amino acid residues;         -   X_(d) is selected from the group of amino acid residues             consisting of S, I, T and V;         -   X_(e) is selected from the group of amino acid residues             consisting of S, A, I and T;         -   X_(f) is selected from the group of amino acid residues             consisting of S, T and V;         -   X_(g) is selected from the group of amino acid residues             consisting of non-bulky amino acid residues;         -   X_(h) is selected from the group of amino acid residues             consisting of S, T and V;         -   X_(i) is selected from the group of amino acid residues             consisting of S, A and G;         -   wherein at least one of the residues X_(b), X_(d), X_(f) and             X_(h) of SEQ ID NO:90 is T or V

    -   41. The polypeptide according to item 40, wherein X_(a) is S.

    -   42. The polypeptide according to item 40, wherein X_(a) is A.

    -   43. The polypeptide according to item 40, wherein X_(a) is G.

    -   44. The polypeptide according to item 40, wherein X_(a) is D.

    -   45. The polypeptide according to item 40, wherein X_(b) is A.

    -   46. The polypeptide according to item 40, wherein X_(b) is V.

    -   47. The polypeptide according to item 40, wherein X_(b) is I.

    -   48. The polypeptide according to item 40, wherein X_(b) is T.

    -   49. The polypeptide according to item 40, wherein X_(b) is S.

    -   50. The polypeptide according to item 40, wherein X_(c) does not         contain a cyclic aliphatic side chain or an aromatic side chain.

    -   51. The polypeptide according to item 40, wherein X_(d) is S.

    -   52. The polypeptide according to item 40, wherein X_(d) is I.

    -   53. The polypeptide according to item 40, wherein X_(d) is T.

    -   54. The polypeptide according to item 40, wherein X_(d) is V.

    -   55. The polypeptide according to item 40, wherein X_(e) is S.

    -   56. The polypeptide according to item 40, wherein X_(e) A.

    -   57. The polypeptide according to item 40, wherein X_(e) I.

    -   58. The polypeptide according to item 40, wherein X_(e) T.

    -   59. The polypeptide according to item 40, wherein X_(f) is S

    -   60. The polypeptide according to item 40, wherein X_(f) is T.

    -   61. The polypeptide according to item 40, wherein X_(f) is V.

    -   62. The polypeptide according to item 40, wherein X_(g) does not         contain a cyclic aliphatic side chain or an aromatic side chain.

    -   63. The polypeptide according to item 40, wherein X_(h) is S.

    -   64. The polypeptide according to item 40, wherein X_(h) is T.

    -   65. The polypeptide according to item 40, wherein X_(h) is V.

    -   66. The polypeptide according to item 40, wherein X_(i) is S.

    -   67. The polypeptide according to item 40, wherein X_(i) is A.

    -   68. The polypeptide according to item 40, wherein X_(i) is G.

    -   69. The polypeptide according to item 40, wherein at least one         of the residues X_(b), X_(d), X_(f) and X_(h) of SEQ ID NO:90 is         T.

    -   70. The polypeptide according to item 40, wherein at least two         of the residues X_(b), X_(d), X_(f) and X_(h) of SEQ ID NO:90         are T.

    -   71. The polypeptide according to item 40, wherein at least three         of the residues X_(b), X_(d), X_(f) and X_(h) of SEQ ID NO:90         are T.

    -   72. The polypeptide according to item 40, wherein all four of         the residues X_(b), X_(d), X_(f) and X_(h) of SEQ ID NO:90 are         T.

    -   73. The polypeptide according to item 40, wherein at least one         of the residues X_(b), X_(d), X_(f) and X_(h) of SEQ ID NO:90 is         V.

    -   74. The polypeptide according to item 40, wherein at least two         of the residues X_(b), X_(d), X_(f) and X_(h) of SEQ ID NO:90         are V.

    -   75. The polypeptide according to item 40, wherein at least three         of the residues X_(b), X_(d), X_(f) and X_(h) of SEQ ID NO:90         are V.

    -   76. The polypeptide according to item 40, wherein all four of         the residues X_(b), X_(d), X_(f) and X_(h) of SEQ ID NO:90 are         V.

    -   77. The polypeptide according to item 40, wherein the first and         second copy of SEQ ID NO:90 are separated by one or more amino         acid residues.

    -   78. The polypeptide according to item 40, wherein the first and         second copies of SEQ ID NO:90 are separated by 2 amino acid         residues, such as 3 amino acid residues, for example 4 amino         acid residues, such as 5 amino acid residues, for example 6         amino acid residues, such as 7 amino acid residues, for example         8 amino acid residues, such as 9 amino acid residues, for         example 10 amino acid residues, such as 11 amino acid residues,         for example 12 amino acid residues, such as 13 amino acid         residues, for example 14 amino acid residues, such as 15 amino         acid residues, for example 16 amino acid residues, such as 17         amino acid residues, for example 18 amino acid residues, such as         19 amino acid residues, for example 20 amino acid residues, such         as 21 amino acid residues, for example 22 amino acid residues,         such as 23 amino acid residues, for example 24 amino acid         residues, such as 25 amino acid residues, for example 26 amino         acid residues, such as 27 amino acid residues, for example 28         amino acid residues, such as 29 amino acid residues, for example         30 amino acid residues.

    -   79. The polypeptide according to item 40, wherein the first and         second copies of

SEQ ID NO:90 are separated by at least 2 amino acid residues, such as at least 3 amino acid residues, for example at least 4 amino acid residues, such as at least 5 amino acid residues, for example at least 6 amino acid residues, such as at least 7 amino acid residues, for example at least 8 amino acid residues, such as at least 9 amino acid residues, for example at least 10 amino acid residues, such as at least 11 amino acid residues, for example at least 12 amino acid residues, such as at least 13 amino acid residues, for example at least 14 amino acid residues, such as at least 15 amino acid residues, for example at least 16 amino acid residues, such as at least 17 amino acid residues, for example at least 18 amino acid residues, such as at least 19 amino acid residues, for example at least 20 amino acid residues, such as at least 21 amino acid residues, for example at least 22 amino acid residues, such as at least 23 amino acid residues, for example at least 24 amino acid residues, such as at least 25 amino acid residues, for example at least 26 amino acid residues, such as at least 27 amino acid residues, for example at least 28 amino acid residues, such as at least 29 amino acid residues, for example at least 30 amino acid residues.

-   -   80. The polypeptide according to item 40, wherein the first and         second copies of SEQ ID NO:90 are separated by less than 100         amino acid residues, such as less than 95 amino acid residues,         for example less than 90 amino acid residues, such as less than         85 amino acid residues, for example less than 80 amino acid         residues, such as less than 75 amino acid residues, for example         less than 70 amino acid residues, such as less than 65 amino         acid residues, for example less than 60 amino acid residues,         such as less than 55 amino acid residues, for example less than         50 amino acid residues, such as less than 45 amino acid         residues, for example less than 40 amino acid residues, such as         less than 35 amino acid residues, for example less than 30 amino         acid residues, such as less than 25 amino acid residues, for         example less than 24 amino acid residues, such as less than 23         amino acid residues, for example less than 22 amino acid         residues, such as less than 21 amino acid residues, for example         less than 20 amino acid residues, such as less than 19 amino         acid residues, for example less than 18 amino acid residues,         such as less than 17 amino acid residues, for example less than         16 amino acid residues, such as less than 15 amino acid         residues, for example less than 14 amino acid residues, such as         less than 13 amino acid residues, for example less than 12 amino         acid residues, such as less than 11 amino acid residues, for         example less than 10 amino acid residues, such as less than 9         amino acid residues, for example less than 8 amino acid         residues, such as less than 7 amino acid residues, for example         less than 6 amino acid residues, such as less than 5 amino acid         residues.     -   81. The polypeptide according to item 40, wherein the first copy         of SEQ ID NO:90 is located N-terminally to the second copy of         SEQ ID NO:90.     -   82. The polypeptide according to item 40, wherein the second         copy of SEQ ID NO:90 is located N-terminally to the first copy         of SEQ ID NO:90.     -   83. The polypeptide according to item 40 further comprising a         third copy of SEQ ID NO:90, which does not overlap with the         first and second copy of SEQ ID NO:90,         -   wherein the third copy of SEQ ID NO:90 comprises the             sequence:

(SEQ ID NO: 90) X_(aa)-X_(ba)-X_(ca)-X_(da)-X_(ea)-X_(fa)-X_(ga)-X_(ha)-X_(ia),

-   -   -   wherein         -   X_(aa) is selected from the group of amino acid residues             consisting of S, A, G and D;         -   X_(ba) is selected from the group of amino acid residues             consisting of A, V, I, T and S;         -   X_(ca) is selected from the group of amino acid residues             consisting of non-bulky amino acid residues;         -   X_(da) is selected from the group of amino acid residues             consisting of S, I, T and V;         -   X_(ea) is selected from the group of amino acid residues             consisting of S, A, I and T;         -   X_(fa) is selected from the group of amino acid residues             consisting of S, T and V;         -   X_(ga) is selected from the group of amino acid residues             consisting of non-bulky amino acid residues;         -   X_(ha) is selected from the group of amino acid residues             consisting of S, T and V;         -   X_(ia) is selected from the group of amino acid residues             consisting of S, A and G;         -   wherein at least one of the residues X_(ba), X_(da), X_(fa)             and X_(ha) of SEQ ID NO:90 is T or V.

    -   84. The polypeptide according to item 83, wherein X_(aa) is S.

    -   85. The polypeptide according to item 83, wherein X_(aa) is A.

    -   86. The polypeptide according to item 83, wherein X_(aa) is G.

    -   87. The polypeptide according to item 83, wherein X_(aa) is D.

    -   88. The polypeptide according to item 83, wherein X_(ba) is A.

    -   89. The polypeptide according to item 83, wherein X_(ba) is V.

    -   90. The polypeptide according to item 83, wherein X_(ba) is I.

    -   91. The polypeptide according to item 83, wherein X_(ba) is T.

    -   92. The polypeptide according to item 83, wherein X_(ba) is S.

    -   93. The polypeptide according to item 83, wherein X_(ca) does         not contain a cyclic aliphatic side chain or an aromatic side         chain.

    -   94. The polypeptide according to item 83, wherein X_(da) is S.

    -   95. The polypeptide according to item 83, wherein X_(da) is I.

    -   96. The polypeptide according to item 83, wherein X_(da) is T.

    -   97. The polypeptide according to item 83, wherein X_(da) is V.

    -   98. The polypeptide according to item 83, wherein X_(ea) is S.

    -   99. The polypeptide according to item 83, wherein X_(ea) A.

    -   100. The polypeptide according to item 83, wherein X_(ea) I.

    -   101. The polypeptide according to item 83, wherein X_(ea) T.

    -   102. The polypeptide according to item 83, wherein X_(fa) is S

    -   103. The polypeptide according to item 83, wherein X_(fa) is T.

    -   104. The polypeptide according to item 83, wherein X_(fa) is V.

    -   105. The polypeptide according to item 83, wherein X_(ga) does         not contain a cyclic aliphatic side chain or an aromatic side         chain.

    -   106. The polypeptide according to item 83, wherein X_(ha) is S.

    -   107. The polypeptide according to item 83, wherein X_(ha) is T.

    -   108. The polypeptide according to item 83, wherein X_(ha) is V.

    -   109. The polypeptide according to item 83, wherein X_(ia) is S.

    -   110. The polypeptide according to item 83, wherein X_(ia) is A.

    -   111. The polypeptide according to item 83, wherein X_(ia) is G.

    -   112. The polypeptide according to item 83, wherein at least one         of the residues X_(ba), X_(da), X_(fa) and X_(ha) of SEQ ID         NO:90 is T.

    -   113. The polypeptide according to item 83, wherein at least two         of the residues X_(ba), X_(da), X_(fa) and X_(ha) of SEQ ID         NO:90 are T.

    -   114. The polypeptide according to item 83, wherein at least         three of the residues X_(ba), X_(da), X_(fa) and X_(ha) of SEQ         ID NO:90 are T.

    -   115. The polypeptide according to item 83, wherein all four of         the residues X_(ba), X_(da), X_(fa) and X_(ha) of SEQ ID NO:90         are T.

    -   116. The polypeptide according to item 83, wherein at least one         of the residues X_(ba), X_(da), X_(fa) and X_(ha) of SEQ ID         NO:90 is V.

    -   117. The polypeptide according to item 83, wherein at least two         of the residues X_(ba), X_(da), X_(fa) and X_(ha) of SEQ ID         NO:90 are V.

    -   118. The polypeptide according to item 83, wherein at least         three of the residues X_(ba), X_(da), X_(fa) and X_(ha) of SEQ         ID NO:90 are V.

    -   119. The polypeptide according to item 83, wherein all four of         the residues X_(ba), X_(da), X_(fa) and X_(ha) of SEQ ID NO:90         are V.

    -   120. The polypeptide according to item 83, wherein the different         copies of SEQ ID NO:90 are separated by one or more amino acid         residues.

    -   121. The polypeptide according to item 83, wherein the second         and the third copy of SEQ ID NO:90 are separated by one or more         amino acid residues.

    -   122. The polypeptide according to item 83, wherein the first         copy of SEQ ID NO:90 and the third copy of SEQ ID NO:90 are         separated by 2 amino acid residues, such as 3 amino acid         residues, for example 4 amino acid residues, such as 5 amino         acid residues, for example 6 amino acid residues, such as 7         amino acid residues, for example 8 amino acid residues, such as         9 amino acid residues, for example 10 amino acid residues, such         as 11 amino acid residues, for example 12 amino acid residues,         such as 13 amino acid residues, for example 14 amino acid         residues, such as 15 amino acid residues, for example 16 amino         acid residues, such as 17 amino acid residues, for example 18         amino acid residues, such as 19 amino acid residues, for example         20 amino acid residues, such as 21 amino acid residues, for         example 22 amino acid residues, such as 23 amino acid residues,         for example 24 amino acid residues, such as 25 amino acid         residues, for example 26 amino acid residues, such as 27 amino         acid residues, for example 28 amino acid residues, such as 29         amino acid residues, for example 30 amino acid residues.

    -   123. The polypeptide according to item 83, wherein the first         copy of SEQ ID NO:90 and the third copy of SEQ ID NO:90 are         separated by at least 2 amino acid residues, such as at least 3         amino acid residues, for example at least 4 amino acid residues,         such as at least 5 amino acid residues, for example at least 6         amino acid residues, such as at least 7 amino acid residues, for         example at least 8 amino acid residues, such as at least 9 amino         acid residues, for example at least 10 amino acid residues, such         as at least 11 amino acid residues, for example at least 12         amino acid residues, such as at least 13 amino acid residues,         for example at least 14 amino acid residues, such as at least 15         amino acid residues, for example at least 16 amino acid         residues, such as at least 17 amino acid residues, for example         at least 18 amino acid residues, such as at least 19 amino acid         residues, for example at least 20 amino acid residues, such as         at least 21 amino acid residues, for example at least 22 amino         acid residues, such as at least 23 amino acid residues, for         example at least 24 amino acid residues, such as at least 25         amino acid residues, for example at least 26 amino acid         residues, such as at least 27 amino acid residues, for example         at least 28 amino acid residues, such as at least 29 amino acid         residues, for example at least 30 amino acid residues.

    -   124. The polypeptide according to item 83, wherein the second         copy of SEQ ID NO:90 and the third copy of SEQ ID NO:90 are         separated by 2 amino acid residues, such as 3 amino acid         residues, for example 4 amino acid residues, such as 5 amino         acid residues, for example 6 amino acid residues, such as 7         amino acid residues, for example 8 amino acid residues, such as         9 amino acid residues, for example 10 amino acid residues, such         as 11 amino acid residues, for example 12 amino acid residues,         such as 13 amino acid residues, for example 14 amino acid         residues, such as 15 amino acid residues, for example 16 amino         acid residues, such as 17 amino acid residues, for example 18         amino acid residues, such as 19 amino acid residues, for example         20 amino acid residues, such as 21 amino acid residues, for         example 22 amino acid residues, such as 23 amino acid residues,         for example 24 amino acid residues, such as 25 amino acid         residues, for example 26 amino acid residues, such as 27 amino         acid residues, for example 28 amino acid residues, such as 29         amino acid residues, for example 30 amino acid residues.

    -   125. The polypeptide according to item 83, wherein the second         copy of SEQ ID NO:90 and the third copy of SEQ ID NO:90 are         separated by at least 2 amino acid residues, such as at least 3         amino acid residues, for example at least 4 amino acid residues,         such as at least 5 amino acid residues, for example at least 6         amino acid residues, such as at least 7 amino acid residues, for         example at least 8 amino acid residues, such as at least 9 amino         acid residues, for example at least 10 amino acid residues, such         as at least 11 amino acid residues, for example at least 12         amino acid residues, such as at least 13 amino acid residues,         for example at least 14 amino acid residues, such as at least 15         amino acid residues, for example at least 16 amino acid         residues, such as at least 17 amino acid residues, for example         at least 18 amino acid residues, such as at least 19 amino acid         residues, for example at least 20 amino acid residues, such as         at least 21 amino acid residues, for example at least 22 amino         acid residues, such as at least 23 amino acid residues, for         example at least 24 amino acid residues, such as at least 25         amino acid residues, for example at least 26 amino acid         residues, such as at least 27 amino acid residues, for example         at least 28 amino acid residues, such as at least 29 amino acid         residues, for example at least 30 amino acid residues.

    -   126. The polypeptide according to item 83, wherein the first         copy of SEQ ID NO:90 and the third copy of SEQ ID NO:90 are         separated by less than 100 amino acid residues, such as less         than 95 amino acid residues, for example less than 90 amino acid         residues, such as less than 85 amino acid residues, for example         less than 80 amino acid residues, such as less than 75 amino         acid residues, for example less than 70 amino acid residues,         such as less than 65 amino acid residues, for example less than         60 amino acid residues, such as less than 55 amino acid         residues, for example less than 50 amino acid residues, such as         less than 45 amino acid residues, for example less than 40 amino         acid residues, such as less than 35 amino acid residues, for         example less than 30 amino acid residues, such as less than 25         amino acid residues, for example less than 24 amino acid         residues, such as less than 23 amino acid residues, for example         less than 22 amino acid residues, such as less than 21 amino         acid residues, for example less than 20 amino acid residues,         such as less than 19 amino acid residues, for example less than         18 amino acid residues, such as less than 17 amino acid         residues, for example less than 16 amino acid residues, such as         less than 15 amino acid residues, for example less than 14 amino         acid residues, such as less than 13 amino acid residues, for         example less than 12 amino acid residues, such as less than 11         amino acid residues, for example less than 10 amino acid         residues, such as less than 9 amino acid residues, for example         less than 8 amino acid residues, such as less than 7 amino acid         residues, for example less than 6 amino acid residues, such as         less than 5 amino acid residues.

    -   127. The polypeptide according to item 83, wherein the second         copy of SEQ ID NO:90 and the third copy of SEQ ID NO:90 are         separated by less than 100 amino acid residues, such as less         than 95 amino acid residues, for example less than 90 amino acid         residues, such as less than 85 amino acid residues, for example         less than 80 amino acid residues, such as less than 75 amino         acid residues, for example less than 70 amino acid residues,         such as less than 65 amino acid residues, for example less than         60 amino acid residues, such as less than 55 amino acid         residues, for example less than 50 amino acid residues, such as         less than 45 amino acid residues, for example less than 40 amino         acid residues, such as less than 35 amino acid residues, for         example less than 30 amino acid residues, such as less than 25         amino acid residues, for example less than 24 amino acid         residues, such as less than 23 amino acid residues, for example         less than 22 amino acid residues, such as less than 21 amino         acid residues, for example less than 20 amino acid residues,         such as less than 19 amino acid residues, for example less than         18 amino acid residues, such as less than 17 amino acid         residues, for example less than 16 amino acid residues, such as         less than 15 amino acid residues, for example less than 14 amino         acid residues, such as less than 13 amino acid residues, for         example less than 12 amino acid residues, such as less than 11         amino acid residues, for example less than 10 amino acid         residues, such as less than 9 amino acid residues, for example         less than 8 amino acid residues, such as less than 7 amino acid         residues, for example less than 6 amino acid residues, such as         less than 5 amino acid residues.

    -   128. The polypeptide according to item 83, wherein the third         copy of SEQ ID NO:90 is located N-terminally to both the first         copy of SEQ ID NO:90 and the second copy of SEQ ID NO:90.

    -   129. The polypeptide according to item 83, wherein the third         copy of SEQ ID NO:90 is located N-terminally to the first copy         of SEQ ID NO:90 and C-terminally to the second copy of SEQ ID         NO:90.

    -   130. The polypeptide according to item 83, wherein the third         copy of SEQ ID NO:90 is located N-terminally to the second copy         of SEQ ID NO:90 and C-terminally to the first copy of SEQ ID         NO:90.

    -   131. The polypeptide according to item 83, wherein the third         copy of SEQ ID NO:90 is located C-terminally to both the first         copy of SEQ ID NO:90 and the second copy of SEQ ID NO:90.

    -   132. The polypeptide according to item 83 further comprising a         fourth copy of SEQ ID NO:90, which does not overlap with any of         the first, second and third copies of SEQ ID NO:90, wherein the         fourth copy of SEQ ID NO:90 comprises the sequence:

(SEQ ID NO: 90) X_(ab)-X_(bb)-X_(cb)-X_(db)-X_(eb)-X_(fb)-X_(gb)-X_(hb)-X_(ib),

-   -   -   wherein         -   X_(ab) is selected from the group of amino acid residues             consisting of S, A, G and D;         -   X_(bb) is selected from the group of amino acid residues             consisting of A, V, I, T and S;         -   X_(cb) is selected from the group of amino acid residues             consisting of non-bulky amino acid residues;         -   X_(db) is selected from the group of amino acid residues             consisting of S, I, T and V;         -   X_(eb) is selected from the group of amino acid residues             consisting of S, A, I and T;         -   X_(fb) is selected from the group of amino acid residues             consisting of S, T and V;         -   X_(gb) is selected from the group of amino acid residues             consisting of non-bulky amino acid residues;         -   X_(hb) is selected from the group of amino acid residues             consisting of S, T and V;         -   X_(ib) is selected from the group of amino acid residues             consisting of S, A and G;         -   wherein at least one of the residues X_(bb), X_(db), X_(fb)             and X_(hb) of SEQ ID NO:90 is T or V.

    -   133. The polypeptide according to item 83, wherein X_(ab) i S.

    -   134. The polypeptide according to item 83, wherein X_(ab) is A.

    -   135. The polypeptide according to item 83, wherein X_(ab) is G.

    -   136. The polypeptide according to item 83, wherein X_(ab) is D.

    -   137. The polypeptide according to item 83, wherein X_(bb) is A.

    -   138. The polypeptide according to item 83, wherein X_(bb) is V.

    -   139. The polypeptide according to item 83, wherein X_(bb) is I.

    -   140. The polypeptide according to item 83, wherein X_(bb) is T.

    -   141. The polypeptide according to item 83, wherein X_(bb) is S.

    -   142. The polypeptide according to item 83, wherein X_(cb) does         not contain a cyclic aliphatic side chain or an aromatic side         chain.

    -   143. The polypeptide according to item 83, wherein X_(db) is S.

    -   144. The polypeptide according to item 83, wherein X_(db) is I.

    -   145. The polypeptide according to item 83, wherein X_(db) is T.

    -   146. The polypeptide according to item 83, wherein X_(db) is V.

    -   147. The polypeptide according to item 83, wherein X_(eb) i S.

    -   148. The polypeptide according to item 83, wherein X_(eb) A.

    -   149. The polypeptide according to item 83, wherein X_(eb) I.

    -   150. The polypeptide according to item 83, wherein X_(eb) T.

    -   151. The polypeptide according to item 83, wherein X_(fb) is S

    -   152. The polypeptide according to item 83, wherein X_(fb) is T.

    -   153. The polypeptide according to item 83, wherein X_(fb) is V.

    -   154. The polypeptide according to item 83, wherein X_(gb) does         not contain a cyclic aliphatic side chain or an aromatic side         chain.

    -   155. The polypeptide according to item 83, wherein X_(hb) is S.

    -   156. The polypeptide according to item 83, wherein X_(hb) is T.

    -   157. The polypeptide according to item 83, wherein X_(hb) is V.

    -   158. The polypeptide according to item 83, wherein X_(ib) is S.

    -   159. The polypeptide according to item 83, wherein X_(ib) is A.

    -   160. The polypeptide according to item 83, wherein X_(ib) is G.

    -   161. The polypeptide according to item 83, wherein at least one         of the residues X_(bb), X_(db), X_(fb) and X_(hb) of SEQ ID         NO:90 is T.

    -   162. The polypeptide according to item 83, wherein at least two         of the residues X_(bb), X_(db), X_(fb) and X_(hb) of SEQ ID         NO:90 are T.

    -   163. The polypeptide according to item 83, wherein at least         three of the residues X_(bb), X_(db), X_(fb) and X_(hb) of SEQ         ID NO:90 are T.

    -   164. The polypeptide according to item 83, wherein all four of         the residues X_(bb), X_(db), X_(fb) and X_(hb) of SEQ ID NO:90         are T.

    -   165. The polypeptide according to item 83, wherein at least one         of the residues X_(bb), X_(db), X_(fb) and X_(hb) of SEQ ID         NO:90 is V.

    -   166. The polypeptide according to item 83, wherein at least two         of the residues X_(bb), X_(db), X_(fb) and X_(hb) of SEQ ID         NO:90 are V.

    -   167. The polypeptide according to item 83, wherein at least         three of the residues X_(bb), X_(db), X_(fb) and X_(hb) of SEQ         ID NO:90 are V.

    -   168. The polypeptide according to item 83, wherein all four of         the residues X_(bb), X_(db), X_(fb) and X_(hb) of SEQ ID NO:90         are V.

    -   169. The polypeptide according to item 83, wherein the first         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by one or more amino acid residues.

    -   170. The polypeptide according to item 83, wherein the second         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by one or more amino acid residues.

    -   171. The polypeptide according to item 83, wherein the third         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by one or more amino acid residues.

    -   172. The polypeptide according to item 83, wherein the first         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by 2 amino acid residues, such as 3 amino acid         residues, for example 4 amino acid residues, such as 5 amino         acid residues, for example 6 amino acid residues, such as 7         amino acid residues, for example 8 amino acid residues, such as         9 amino acid residues, for example 10 amino acid residues, such         as 11 amino acid residues, for example 12 amino acid residues,         such as 13 amino acid residues, for example 14 amino acid         residues, such as 15 amino acid residues, for example 16 amino         acid residues, such as 17 amino acid residues, for example 18         amino acid residues, such as 19 amino acid residues, for example         20 amino acid residues, such as 21 amino acid residues, for         example 22 amino acid residues, such as 23 amino acid residues,         for example 24 amino acid residues, such as 25 amino acid         residues, for example 26 amino acid residues, such as 27 amino         acid residues, for example 28 amino acid residues, such as 29         amino acid residues, for example 30 amino acid residues.

    -   173. The polypeptide according to item 83, wherein the second         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by 2 amino acid residues, such as 3 amino acid         residues, for example 4 amino acid residues, such as 5 amino         acid residues, for example 6 amino acid residues, such as 7         amino acid residues, for example 8 amino acid residues, such as         9 amino acid residues, for example 10 amino acid residues, such         as 11 amino acid residues, for example 12 amino acid residues,         such as 13 amino acid residues, for example 14 amino acid         residues, such as 15 amino acid residues, for example 16 amino         acid residues, such as 17 amino acid residues, for example 18         amino acid residues, such as 19 amino acid residues, for example         20 amino acid residues, such as 21 amino acid residues, for         example 22 amino acid residues, such as 23 amino acid residues,         for example 24 amino acid residues, such as 25 amino acid         residues, for example 26 amino acid residues, such as 27 amino         acid residues, for example 28 amino acid residues, such as 29         amino acid residues, for example 30 amino acid residues.

    -   174. The polypeptide according to item 83, wherein the third         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by 2 amino acid residues, such as 3 amino acid         residues, for example 4 amino acid residues, such as 5 amino         acid residues, for example 6 amino acid residues, such as 7         amino acid residues, for example 8 amino acid residues, such as         9 amino acid residues, for example 10 amino acid residues, such         as 11 amino acid residues, for example 12 amino acid residues,         such as 13 amino acid residues, for example 14 amino acid         residues, such as 15 amino acid residues, for example 16 amino         acid residues, such as 17 amino acid residues, for example 18         amino acid residues, such as 19 amino acid residues, for example         20 amino acid residues, such as 21 amino acid residues, for         example 22 amino acid residues, such as 23 amino acid residues,         for example 24 amino acid residues, such as 25 amino acid         residues, for example 26 amino acid residues, such as 27 amino         acid residues, for example 28 amino acid residues, such as 29         amino acid residues, for example 30 amino acid residues.

    -   175. The polypeptide according to item 83, wherein the first         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by at least 2 amino acid residues, such as at least 3         amino acid residues, for example at least 4 amino acid residues,         such as at least 5 amino acid residues, for example at least 6         amino acid residues, such as at least 7 amino acid residues, for         example at least 8 amino acid residues, such as at least 9 amino         acid residues, for example at least 10 amino acid residues, such         as at least 11 amino acid residues, for example at least 12         amino acid residues, such as at least 13 amino acid residues,         for example at least 14 amino acid residues, such as at least 15         amino acid residues, for example at least 16 amino acid         residues, such as at least 17 amino acid residues, for example         at least 18 amino acid residues, such as at least 19 amino acid         residues, for example at least 20 amino acid residues, such as         at least 21 amino acid residues, for example at least 22 amino         acid residues, such as at least 23 amino acid residues, for         example at least 24 amino acid residues, such as at least 25         amino acid residues, for example at least 26 amino acid         residues, such as at least 27 amino acid residues, for example         at least 28 amino acid residues, such as at least 29 amino acid         residues, for example at least 30 amino acid residues.

    -   176. The polypeptide according to item 83, wherein the second         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by at least 2 amino acid residues, such as at least 3         amino acid residues, for example at least 4 amino acid residues,         such as at least 5 amino acid residues, for example at least 6         amino acid residues, such as at least 7 amino acid residues, for         example at least 8 amino acid residues, such as at least 9 amino         acid residues, for example at least 10 amino acid residues, such         as at least 11 amino acid residues, for example at least 12         amino acid residues, such as at least 13 amino acid residues,         for example at least 14 amino acid residues, such as at least 15         amino acid residues, for example at least 16 amino acid         residues, such as at least 17 amino acid residues, for example         at least 18 amino acid residues, such as at least 19 amino acid         residues, for example at least 20 amino acid residues, such as         at least 21 amino acid residues, for example at least 22 amino         acid residues, such as at least 23 amino acid residues, for         example at least 24 amino acid residues, such as at least 25         amino acid residues, for example at least 26 amino acid         residues, such as at least 27 amino acid residues, for example         at least 28 amino acid residues, such as at least 29 amino acid         residues, for example at least 30 amino acid residues.

    -   177. The polypeptide according to item 83, wherein the third         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by at least 2 amino acid residues, such as at least 3         amino acid residues, for example at least 4 amino acid residues,         such as at least 5 amino acid residues, for example at least 6         amino acid residues, such as at least 7 amino acid residues, for         example at least 8 amino acid residues, such as at least 9 amino         acid residues, for example at least 10 amino acid residues, such         as at least 11 amino acid residues, for example at least 12         amino acid residues, such as at least 13 amino acid residues,         for example at least 14 amino acid residues, such as at least 15         amino acid residues, for example at least 16 amino acid         residues, such as at least 17 amino acid residues, for example         at least 18 amino acid residues, such as at least 19 amino acid         residues, for example at least 20 amino acid residues, such as         at least 21 amino acid residues, for example at least 22 amino         acid residues, such as at least 23 amino acid residues, for         example at least 24 amino acid residues, such as at least 25         amino acid residues, for example at least 26 amino acid         residues, such as at least 27 amino acid residues, for example         at least 28 amino acid residues, such as at least 29 amino acid         residues, for example at least 30 amino acid residues.

    -   178. The polypeptide according to item 83, wherein the first         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by less than 100 amino acid residues, such as less         than 95 amino acid residues, for example less than 90 amino acid         residues, such as less than 85 amino acid residues, for example         less than 80 amino acid residues, such as less than 75 amino         acid residues, for example less than 70 amino acid residues,         such as less than 65 amino acid residues, for example less than         60 amino acid residues, such as less than 55 amino acid         residues, for example less than 50 amino acid residues, such as         less than 45 amino acid residues, for example less than 40 amino         acid residues, such as less than 35 amino acid residues, for         example less than 30 amino acid residues, such as less than 25         amino acid residues, for example less than 24 amino acid         residues, such as less than 23 amino acid residues, for example         less than 22 amino acid residues, such as less than 21 amino         acid residues, for example less than 20 amino acid residues,         such as less than 19 amino acid residues, for example less than         18 amino acid residues, such as less than 17 amino acid         residues, for example less than 16 amino acid residues, such as         less than 15 amino acid residues, for example less than 14 amino         acid residues, such as less than 13 amino acid residues, for         example less than 12 amino acid residues, such as less than 11         amino acid residues, for example less than 10 amino acid         residues, such as less than 9 amino acid residues, for example         less than 8 amino acid residues, such as less than 7 amino acid         residues, for example less than 6 amino acid residues, such as         less than 5 amino acid residues.

    -   179. The polypeptide according to item 83, wherein the second         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by less than 100 amino acid residues, such as less         than 95 amino acid residues, for example less than 90 amino acid         residues, such as less than 85 amino acid residues, for example         less than 80 amino acid residues, such as less than 75 amino         acid residues, for example less than 70 amino acid residues,         such as less than 65 amino acid residues, for example less than         60 amino acid residues, such as less than 55 amino acid         residues, for example less than 50 amino acid residues, such as         less than 45 amino acid residues, for example less than 40 amino         acid residues, such as less than 35 amino acid residues, for         example less than 30 amino acid residues, such as less than 25         amino acid residues, for example less than 24 amino acid         residues, such as less than 23 amino acid residues, for example         less than 22 amino acid residues, such as less than 21 amino         acid residues, for example less than 20 amino acid residues,         such as less than 19 amino acid residues, for example less than         18 amino acid residues, such as less than 17 amino acid         residues, for example less than 16 amino acid residues, such as         less than 15 amino acid residues, for example less than 14 amino         acid residues, such as less than 13 amino acid residues, for         example less than 12 amino acid residues, such as less than 11         amino acid residues, for example less than 10 amino acid         residues, such as less than 9 amino acid residues, for example         less than 8 amino acid residues, such as less than 7 amino acid         residues, for example less than 6 amino acid residues, such as         less than 5 amino acid residues.

    -   180. The polypeptide according to item 83, wherein the third         copy of SEQ ID NO:90 and the fourth copy of SEQ ID NO:90 are         separated by less than 100 amino acid residues, such as less         than 95 amino acid residues, for example less than 90 amino acid         residues, such as less than 85 amino acid residues, for example         less than 80 amino acid residues, such as less than 75 amino         acid residues, for example less than 70 amino acid residues,         such as less than 65 amino acid residues, for example less than         60 amino acid residues, such as less than 55 amino acid         residues, for example less than 50 amino acid residues, such as         less than 45 amino acid residues, for example less than 40 amino         acid residues, such as less than 35 amino acid residues, for         example less than 30 amino acid residues, such as less than 25         amino acid residues, for example less than 24 amino acid         residues, such as less than 23 amino acid residues, for example         less than 22 amino acid residues, such as less than 21 amino         acid residues, for example less than 20 amino acid residues,         such as less than 19 amino acid residues, for example less than         18 amino acid residues, such as less than 17 amino acid         residues, for example less than 16 amino acid residues, such as         less than 15 amino acid residues, for example less than 14 amino         acid residues, such as less than 13 amino acid residues, for         example less than 12 amino acid residues, such as less than 11         amino acid residues, for example less than 10 amino acid         residues, such as less than 9 amino acid residues, for example         less than 8 amino acid residues, such as less than 7 amino acid         residues, for example less than 6 amino acid residues, such as         less than 5 amino acid residues.

    -   181. The polypeptide according to item 83, wherein the fourth         copy of SEQ ID NO:90 is located N-terminally to the first copy         of SEQ ID NO:90.

    -   182. The polypeptide according to item 83, wherein the fourth         copy of SEQ ID NO:90 is located C-terminally to the first copy         of SEQ ID NO:90.

    -   183. The polypeptide according to item 83, wherein the fourth         copy of SEQ ID NO:90 is located N-terminally to the second copy         of SEQ ID NO:90.

    -   184. The polypeptide according to item 83, wherein the fourth         copy of SEQ ID NO:90 is located C-terminally to the second copy         of SEQ ID NO:90.

    -   185. The polypeptide according to item 83, wherein the fourth         copy of SEQ ID NO:90 is located N-terminally to the third copy         of SEQ ID NO:90.

    -   186. The polypeptide according to item 83, wherein the fourth         copy of SEQ ID NO:90 is located C-terminally to the third copy         of SEQ ID NO:90.

    -   187. The polypeptide according to any of items 1 to 186 attached         to a carrier.

    -   188. The polypeptide according to item 187 wherein the carrier         comprises an avidin moiety, such as streptavidin, which is         optionally biotinylated.

    -   189. The polypeptide according to any of items 1 to 186         attached, such as covalently bound, to a solid support or a         semi-solid support.

    -   190. The polypeptide according to any of items 1 to 186 operably         fused to an affinity tag, such as a His-tag.

    -   191. A fusion polypeptide comprising the polypeptide according         to any of items 1 to 186 operably fused to an N-terminal         flanking sequence.

    -   192. A fusion polypeptide comprising the polypeptide according         to any of items 1 to 186 operably fused to an C-terminal         flanking sequence.

    -   193. The polypeptide according to any of items 1 to 186 operably         fused to a signal peptide.

    -   194. The polypeptide according to any of items 1 to 186 operably         fused to a pro-region.

    -   195. The polypeptide according to any of items 1 to 186 operably         fused to a pre-pro-region.

    -   196. The polypeptide according to any of items 1 to 186, wherein         one or more amino acid residues are modified, said         modification(s) preferably being selected from the group         consisting of in vivo or in vitro chemical derivatization, such         as acetylation or carboxylation, glycosylation, such as         glycosylation resulting from exposing the polypeptide to enzymes         which affect glycosylation, for example mammalian glycosylating         or deglycosylating enzymes, phosphorylation, such as         modification of amino acid residues which results in         phosphorylated amino acid residues, for example phosphotyrosine,         phosphoserine and phosphothreonine.

    -   197. The polypeptide according to any of items 1 to 186, wherein         one or more amino acid residues are modified so as to preferably         improve the resistance to proteolytic degradation and stability         or to optimize solubility properties or to render the         polypeptide more suitable as a therapeutic agent.

    -   198. The polypeptide according to item 197 comprising amino acid         residues other than naturally occurring L-amino acid residues.

    -   199. The polypeptide according to item 198 comprising D-amino         acid residues.

    -   200. The polypeptide according to item 198 comprising         non-naturally occurring, synthetic amino acids.

    -   201. The polypeptide according to item 197 comprising one or         more blocking groups preferably in the form of chemical         substituents suitable to protect and/or stabilize the N- and         C-termini of the polypeptide from undesirable degradation.

    -   202. The polypeptide according to item 201, wherein the one or         more blocking groups include protecting groups which do not         adversely affect in vivo activities of the polypeptide.

    -   203. The polypeptide according to item 201, wherein the one or         more blocking groups are introduced by alkylation or acylation         of the N-terminus.

    -   204. The polypeptide according to item 201, wherein the one or         more blocking groups are selected from N-terminal blocking         groups comprising C₁ to C₅ branched or non-branched alkyl groups         and acyl groups, such as formyl and acetyl groups, as well as         substituted forms thereof, such as the acetamidomethyl (Acm)         group.

    -   205. The polypeptide according to item 201, wherein the one or         more blocking groups are selected from N-terminal blocking         groups comprising desamino analogs of amino acids, which are         either coupled to the N-terminus of the peptide or used in place         of the N-terminal amino acid residue.

    -   206. The polypeptide according to item 201, wherein the one or         more blocking groups are selected from C-terminal blocking         groups wherein the carboxyl group of the C-terminus is either         incorporated or not, such as esters, ketones, and amides, as         well as descarboxylated amino acid analogues.

    -   207. The polypeptide according to item 201, wherein the one or         more blocking groups are selected from C-terminal blocking         groups comprising ester or ketone-forming alkyl groups, such as         lower (C₁ to C₆) alkyl groups, for example methyl, ethyl and         propyl, and amide-forming amino groups, such as primary amines         (—NH₂), and mono- and di-alkylamino groups, such as methylamino,         ethylamino, dimethylamino, diethylamino, methylethylamino, and         the like.

    -   208. The polypeptide according to item 201, wherein free amino         group(s) at the N-terminal end and free carboxyl group(s) at the         termini can be removed altogether from the polypeptide to yield         desamino and descarboxylated forms thereof without significantly         affecting the biological activity of the polypeptide.

    -   209. An acid addition salt of the polypeptide according to any         of items 1 to 208, said salt being obtainable by treating the         polypeptide with an inorganic acid, such as hydrochloric acid,         hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid,         and the like, or an organic acid such as an acetic acid,         propionic acid, glycolic acid, pyruvic acid, oxalic acid, malic         acid, malonic acid, succinic acid, maleic acid, fumaric acid,         tartaric acid, citric acid, benzoic acid, cinnamic acid,         mandelic acid, methanesulfonic acid, ethanesulfonic acid,         p-toluenesulfonic acid, or salicylic acid, to provide a water         soluble salt of the polypeptide.

    -   210. A method for producing the polypeptide according to any of         items 1 to 208, said method comprising the steps of providing a         polynucleotide encoding said polypeptide and expressing said         polynucleotide either in vitro, or in vivo in a suitable host         organism, thereby producing the polypeptide according to any of         items 1 to 208.

    -   211. A polynucleotide encoding at polypeptide part of the         polypeptide according to any of items 1 to 208.

    -   212. An expression vector comprising the polynucleotide         according to item 211, said polynucleotide being optionally         operably linked to regulatory sequence controlling the         expression of said polynucleotide in a suitable host cell.

    -   213. A recombinant or transgenic host cell comprising the         polypeptide according to any of items 1 to 208 and/or the         polynucleotide according to item 211 and/or the expression         vector according to item 212.

    -   214. A method for generating a recombinant or transgenic host         cell, said method comprising the steps of providing a         polynucleotide encoding a polypeptide according to any of items         1 to 208, introducing said polynucleotide into said recombinant         or transgenic host cell and optionally also expressing said         polynucleotide in said recombinant or transgenic host cell,         thereby generating a recombinant or transgenic host cell         producing said polypeptide.

    -   215. A transgenic, mammalian organism comprising the host cell         according to item 213.

    -   216. The transgenic, mammalian organism according to item 215,         wherein said mammalian host cell is an animal cell selected from         the monophyletic group Bilateria, including a mammalian cell         belonging to any of the four major lineages Deuterostomes,         Ecdysozoa, Platyzoa and Lophotrochozoa.

    -   217. The transgenic, mammalian organism according to item 215,         wherein said mammalian host cell is an animal cell selected from         the group consisting of a Blastomere cell, an Egg cell, an         Embryonic stem cell, an Erythrocyte, a Fibroblast, a Hepatocyte,         a Myoblast, a Myotube, a Neuron, an Oocyte, an Osteoblast, an         Osteoclast, a Sperm cell, a T-Cell and a Zygote.

    -   218. A method for generating a transgenic, mammalian host cell,         said method comprising the steps of providing a polynucleotide         encoding a polypeptide according to any of items 1 to 208,         introducing said polynucleotide into said recombinant or         transgenic host cell and optionally also expressing said         polynucleotide in said transgenic, mammalian host cell, thereby         generating a transgenic, mammalian host cell producing said         polypeptide.

    -   219. A transgenic fish comprising the polypeptide according to         any of items 1 to 208 and/or the polynucleotide according to         item 211 and/or the expression vector according to item 212.

    -   220. The transgenic fish according to item 219, wherein said         fish is a salmon

    -   221. The transgenic fish according to item 219, wherein said         fish is a flounder

    -   222. The transgenic fish according to item 219, wherein said         fish is a cod

    -   223. The transgenic fish according to item 219, wherein said         fish is a herring.

    -   224. A transgenic plant comprising the host cell according to         item 213.

    -   225. The transgenic, plant host cell according to item 224,         wherein said host cell is a plant cell of the taxon Embryophyta         or Viridiplantae or Chlorobionta, preferably selected from the         group consisting of Aleurone cells, Collenchyma cells,         Endodermis cells, Endosperm cells, Epidermis cells, Mesophyll         cells, Meristematic cells, Palisade cells, Parenchyma cells,         Phloem sieve tube cells, Pollen generative cells, Pollen         vegetative cells, Sclerenchyma cells, Tracheids cells, Xylem         vessel cells and Zygote cells.

    -   226. The transgenic plant according to item 224, wherein said         plant is a potato plant.

    -   227. The transgenic plant according to item 224, wherein said         plant is a tomato plant.

    -   228. The transgenic plant according to item 224, wherein said         plant is a grape vine.

    -   229. The transgenic plant according to item 224, wherein said         plant is a cucumber plant.

    -   230. The transgenic plant according to item 224, wherein said         plant is wheat.

    -   231. The transgenic plant according to item 224, wherein said         plant is a barley.

    -   232. The transgenic plant according to item 224, wherein said         plant is rye.

    -   233. The transgenic plant according to item 224, wherein said         plant is oats.

    -   234. The transgenic plant according to item 224, wherein said         plant is a tobacco plant.

    -   235. The transgenic plant according to item 224, wherein said         plant is a citrus plant.

    -   236. The transgenic plant according to item 224, wherein said         plant is an apple plant.

    -   237. The transgenic plant according to item 224, wherein said         plant is a strawberry plant.

    -   238. The transgenic plant according to item 224, wherein said         plant is a raspberry plant.

    -   239. A method for generating a transgenic plant, said method         comprising the steps of providing a polynucleotide encoding a         polypeptide according to any of items 1 to 208, introducing said         polynucleotide into said plant and optionally also expressing         said polynucleotide in said plant, thereby generating a         transgenic plant producing said polypeptide.

    -   240. A recombinant bacterial host cell comprising the         polypeptide according to any of items 1 to 208 and/or the         polynucleotide according to item 211 and/or the vector according         to item 212.

    -   241. The bacterial host cell according to item 240, wherein said         bacterial host cell is selected from a Gram-positive bacterial         host cell and a Gram-negative bacterial host cell.

    -   242. The bacterial host cell according to item 240, wherein said         bacterial cell is a strain of Lactobacillus.

    -   243. The bacterial host cell according to item 240, wherein said         bacterial cell is a strain of Streptococcus.

    -   244. The bacterial host cell according to item 240, wherein said         bacterial cell is a strain of Bifidobacterium.

    -   245. A method for generating a recombinant bacterial cell, said         method comprising the steps of providing a polynucleotide         encoding a polypeptide according to any of items 1 to 208,         introducing said polynucleotide into said bacterial cell and         optionally also expressing said polynucleotide in said bacterial         cell, thereby generating a recombint bacterial cell producing         said polypeptide.

    -   246. A recombinant yeast cell comprising the polypeptide         according to any of items 1 to 208 and/or the polynucleotide         according to item 211 and/or the vector according to item 212.

    -   247. The yeast host cell according to item 246, wherein said         yeast host cell belongs to the genera of Saccharomyces,         Scizosacchomyces or Pichia.

    -   248. The yeast host cell according to item 246, wherein said         yeast is a Saccharomyces cerevisiae.

    -   249. The yeast host cell according to item 246, wherein said         yeast is a Scizosacchomyces pompe.

    -   250. The yeast host cell according to item 246, wherein said         yeast is a Pichia pastoris.

    -   251. A method for generating a recombinant yeast cell, said         method comprising the steps of providing a polynucleotide         encoding a polypeptide according to any of items 1 to 208,         introducing said polynucleotide into said yeast cell and         optionally also expressing said polynucleotide in said yeast         cell, thereby generating a recombint yeast cell producing said         polypeptide.

    -   252. A recombinant fungal host cell comprising the polypeptide         according to any of items 1 to 208 and/or the polynucleotide         according to item 211 and/or the vector according to item 212.

    -   253. The fungal host cell according to item 252, wherein said         fungal cell belongs to the genus of Aspergillus.

    -   254. A method for generating a recombinant fungal cell, said         method comprising the steps of providing a polynucleotide         encoding a polypeptide according to any of items 1 to 208,         introducing said polynucleotide into said fungal cell and         optionally also expressing said polynucleotide in said fungal         cell, thereby generating a recombint bacterial cell producing         said polypeptide.

    -   255. An antibody, or a binding fragment thereof, specific for         the polypeptide according to any of items 1 to 208.

    -   256. The antibody according to item 255, wherein said antibody         is polyclonal.

    -   257. The antibody according to item 255, wherein said antibody         is monoclonal.

    -   258. The antibody fragment according to item 255, wherein said         antibody fragment comprises a portion of an antibody selected         from the group consisting of F(ab′)₂, F(ab)₂, Fab′ and Fab.

    -   259. The antibody fragment according to item 255, wherein said         antibody fragment is synthetic or a genetically engineered         polypeptide that binds to a specific antigen.

    -   260. The antibody fragment according to item 255, wherein said         antibody fragment is selected from the group consisting of         antibody fragments comprising or consisting of the light chain         variable region, antibody fragments comprising or consisting of         a “Fv” fragment consisting of the variable regions of the heavy         and light chains, antibody fragments comprising or consisting of         recombinant single chain polypeptide molecules in which light         and heavy variable regions are connected by a peptide linker         (“scFv polypeptides”) and antibody fragments comprising or         consisting of minimal recognition units consisting of the amino         acid residues that mimic the hypervariable region.

    -   261. The antibody according to item 255, wherein said antibody         is a chimeric antibody in the form of a recombinant polypeptide         that contains the variable domains and complementary determining         regions derived from a rodent antibody, while the remainder of         the antibody molecule is derived from a human antibody.

    -   262. The antibody according to item 255, wherein said antibody         is a humanized antibody in the form of a recombinant polypeptide         in which murine complementarity determining regions of a         monoclonal antibody have been transferred from heavy and light         variable chains of the murine immunoglobulin into a human         variable domain.

    -   263. The antibody according to any of items 255 to 262 further         comprising or being associated with a detectable label in the         form of a molecule or atom which can be conjugated to an         antibody moiety to produce a moiety which can be more easily         detected.

    -   264. The antibody according to item 263, wherein the label is         selected from the group consisting of chelators, photoactive         agents, radioisotopes, fluorescent agents and paramagnetic ions.

    -   265. A method for generating a polyclonal antibody, or a binding         fragment thereof specific for the polypeptide according to any         of items 1 to 208, said method comprising the steps of         immunizing a mammalian subject with the polypeptide according to         any of items 1 to 208 under conditions eliciting an antibody         response, identifying an antibody which bind specifically to the         polypeptide, and optionally isolating said antibody or binding         fragment thereof from said mammalian subject.

    -   266. A method for generating a monoclonal antibody specific for         the polypeptide according to any of items 1 to 208, said method         comprising the steps of immunizing a mammalian subject with the         polypeptide according to any of items 1 to 208 under conditions         eliciting an antibody response, preparing a hybridoma producing         a monoclonal antibody specific for the polypeptide according to         any of items 1 to 208, and identifying an antibody which bind         specifically to the polypeptide.

    -   267. A composition comprising the polypeptide according to any         of items 1 to 208 and a carrier.

    -   268. An ice repelling surface comprising the polypeptide         according to any of items 1 to 208 or the composition according         to item 267.

    -   269. A refrigerator comprising the ice repelling surface         according to item 268.

    -   270. A freezer comprising the ice repelling surface according to         item 268.

    -   271. A window comprising the ice repelling surface according to         item 268.

    -   272. A windmill wing comprising the ice repelling surface         according to item 268.

    -   273. A radio detection and ranging device (radar) comprising the         ice repelling surface according to item 268.

    -   274. An automobile comprising the ice repelling surface         according to item 268.

    -   275. A heat pump comprising the ice repelling surface according         to item 268.

    -   276. A sailing vessel comprising the ice repelling surface         according to item 268.

    -   277. A road surface comprising the ice repelling surface         according to item 268.

    -   278. A pipe for diverting a liquid source, such as water, said         pipe comprising the ice repelling surface according to item 268.

    -   279. The pipe according to item 278 made of plastic.

    -   280. The pipe according to item 278 made of metal.

    -   281. A roof construction comprising the ice repelling surface         according to item 268.

    -   282. A bottle comprising the ice repelling surface according to         item 268.

    -   283. A can comprising the ice repelling surface according to         item 268.

    -   284. An antenna device comprising the ice repelling surface         according to item 268.

    -   285. A windshield wiper comprising the ice repelling surface         according to item 268.

    -   286. A rubber tire comprising the ice repelling surface         according to item 268.

    -   287. An air-conditioning installation comprising the ice         repelling surface according to item 268.

    -   288. A railroad track comprising the ice repelling surface         according to item 268.

    -   289. A train wheel comprising the ice repelling surface         according to item 268.

    -   290. A power cable comprising the ice repelling surface         according to item 268.

    -   291. An ice nucleating surface comprising the polypeptide         according to any of items 1 to 208 or the composition according         to item 267.

    -   292. A refrigerator comprising the ice nucleating surface         according to item 291.

    -   293. A freezer comprising the ice nucleating surface according         to item 291.

    -   294. A window comprising the ice nucleating surface according to         item 291.

    -   295. A windmill wing comprising the ice nucleating surface         according to item 291.

    -   296. A radio detection and ranging device (radar) comprising the         ice nucleating surface according to item 291.

    -   297. An automobile comprising the ice nucleating surface         according to item 291.

    -   298. A heat pump comprising the ice nucleating surface according         to item 291.

    -   299. A sailing vessel comprising the ice nucleating surface         according to item 291.

    -   300. A road surface comprising the ice nucleating surface         according to item 291.

    -   301. A pipe for diverting a liquid source, such as water, said         pipe comprising the ice nucleating surface according to item         291.

    -   302. The pipe according to item 301 made of plastic.

    -   303. The pipe according to item 301 made of metal.

    -   304. A roof construction comprising the ice nucleating surface         according to item 291.

    -   305. A bottle comprising the ice nucleating surface according to         item 291.

    -   306. A can comprising the ice nucleating surface according to         item 291.

    -   307. An antenna device comprising the ice nucleating surface         according to item 291.

    -   308. A windshield wiper comprising the ice nucleating surface         according to item 291.

    -   309. A rubber tire comprising the ice nucleating surface         according to item 291.

    -   310. An air-conditioning installation comprising the ice         nucleating surface according to item 291.

    -   311. A railroad track comprising the ice nucleating surface         according to item 291.

    -   312. A train wheel comprising the ice nucleating surface         according to item 291.

    -   313. A power cable comprising the ice nucleating surface         according to item 291.

    -   314. A method for lowering the freezing point of an aqueous,         liquid composition, said method comprising the steps of         contacting the polypeptide according to any of items 1 to 209         and said aqueous, liquid composition, wherein said contacting         results in lowering the freezing point of said aqueous, liquid         composition.

    -   315. The method of item 314, wherein said liquid composition         comprises a paint composition.

    -   316. The method of item 314, wherein said liquid composition         comprises an anti-freeze for use in freezers.

    -   317. The method of item 314, wherein said liquid composition         comprises an anti-freeze for use in refrigerators.

    -   318. The method of item 314, wherein said liquid composition         comprises an anti-freeze for use in engines.

    -   319. The method of item 314, wherein said liquid composition         comprises a windshield wash.

    -   320. The method of item 314, wherein said liquid composition         comprises a silicone.

    -   321. The method of item 314, wherein said liquid composition         comprises a coating composition, such as a paint, a lacquer or a         varnish.

    -   322. The method of item 314, wherein said liquid composition         comprises a vax.

    -   323. The method of item 314, wherein said liquid composition         comprises an oil.

    -   324. The method of item 314, wherein said liquid composition         comprises cement.

    -   325. The method of item 314, wherein said liquid composition         comprises concrete.

    -   326. The method of item 314, wherein said liquid composition         comprises a soap.

    -   327. The method of item 314, wherein said liquid composition         comprises an anti-freeze composition for use in a lock.

    -   328. A method for reducing or eliminating recrystallisation of         an aqueous, liquid composition, said method comprising the step         of contacting the polypeptide according to any of items 1 to 209         and the aqueous liquid composition prior to freezing, thereby         reducing or eliminating recrystallisation of the aqueous, liquid         composition.

    -   329. The method of item 328, wherein said liquid composition         comprises a paint composition.

    -   330. The method of item 328, wherein said liquid composition         comprises an anti-freeze for use in freezers.

    -   331. The method of item 328, wherein said liquid composition         comprises an anti-freeze for use in refrigerators.

    -   332. The method of item 328, wherein said liquid composition         comprises an anti-freeze for use in engines.

    -   333. The method of item 328, wherein said liquid composition         comprises a windshield wash.

    -   334. The method of item 328, wherein said liquid composition         comprises a silicone.

    -   335. The method of item 328, wherein said liquid composition         comprises a lacquer.

    -   336. The method of item 328, wherein said liquid composition         comprises a vax.

    -   337. The method of item 328, wherein said liquid composition         comprises an oil.

    -   338. The method of item 328, wherein said liquid composition         comprises cement.

    -   339. The method of item 328, wherein said liquid composition         comprises concrete.

    -   340. The method of item 328, wherein said liquid composition         comprises a soap.

    -   341. The method of item 328, wherein said liquid composition         comprises an anti-freeze composition for use in a lock.

    -   342. A method for preserving and/or lowering the freezing point         of a biological sample or an organ by contacting the polypeptide         according to items 1 to 209 and the biological sample or the         organ, thereby allowing storage of the biological sample or the         organ in a super cooled condition or a frozen condition.

    -   343. The method of item 342, wherein the biological sample         comprises polypeptide

    -   344. The method of item 342, wherein the biological sample         comprises microsomes or micelles.

    -   345. The method of item 342, wherein the biological sample         comprises whole blood.

    -   346. The method of item 342, wherein the biological sample         comprises blood plasma.

    -   347. The method of item 342, wherein the biological sample         comprises blood platelets.

    -   348. The method of item 342, wherein the biological sample         comprises red blood cells.

    -   349. The method of item 342, wherein the biological sample         comprises semen.

    -   350. The method of item 342, wherein the biological sample         comprises gametes.

    -   351. The method of item 342, wherein said sample comprises a         cell culture of insect cells.

    -   352. The method of item 342, wherein said sample comprises a         cell culture of mammalian cells.

    -   353. The method of item 352, wherein the mammalian cells are         rodent cells.

    -   354. The method of item 352, wherein the mammalian cells are         human cells.

    -   355. The method of item 352, wherein said biological sample         comprises or consists of an organ.

    -   356. The method of items 355, wherein the organ is selected from         the group consisting of kidney, a lung, a heart, a spleen, and a         liver.

    -   357. A method for inhibiting recrystallization of a biological         sample or an organ during storage thereof, said method         comprising the step of contacting the polypeptide according to         items 1 to 209 to said biological sample or organ, thereby         inhibiting recrystallization and allowing storage of the         biological sample or organ in a super cooled condition or a         frozen condition.

    -   358. The method of item 357, wherein the biological sample         comprises polypeptide

    -   359. The method of item 357, wherein the biological sample         comprises microsomes or micelles.

    -   360. The method of item 357, wherein the biological sample         comprises whole blood.

    -   361. The method of item 357, wherein the biological sample         comprises blood plasma.

    -   362. The method of item 357, wherein the biological sample         comprises blood platelets.

    -   363. The method of item 357, wherein the biological sample         comprises red blood cells.

    -   364. The method of item 357, wherein the biological sample         comprises semen.

    -   365. The method of item 357, wherein the biological sample         comprises gametes.

    -   366. The method of item 357, wherein said sample comprises a         cell culture of insect cells.

    -   367. The method of item 357, wherein said sample comprises a         cell culture of mammalian cells.

    -   368. The method of item 367, wherein the mammalian cells are         rodent cells.

    -   369. The method of item 367, wherein the mammalian cells are         human cells.

    -   370. The method of item 357, wherein said biological sample         comprises or consists of an organ.

    -   371. The method of items 370, wherein the organ is selected from         the group consisting of kidney, a lung, a heart, a spleen, and a         liver.

    -   372. A method for preserving an edible or drinkable composition,         such as a food, by contacting the food with the polypeptide         according to any of items 1 to 209, thereby allowing improved or         prolonged storage of the edible or drinkable composition in a         non-frozen state at a temperature at which the food would         otherwise be in a frozen state.

    -   373. The method of item 372, wherein the composition is selected         from the group consisting of milk, a fermented milk product,         cheese, minced meat, minced fish, yoghurt, sorbet, sherbet,         pudding, a vegetable puree, a fruit puree, a dough, ice milk,         custard, water-ices, slush ice, smoothies, ice cream, granitas,         paste and meat.

    -   374. A method for reducing or inhibiting recrystallisation of         ice crystals on an edible or drinkable composition, such as a         food, by contacting the food with the polypeptide according to         any of items 1 to 209, thereby reducing or inhibiting         recrystallisation of ice crystals formed on the composition         during storage at a temperature at which ice crystals would         otherwise have been formed.

    -   375. The method of item 374, wherein the composition is selected         from the group consisting of milk, a fermented milk product,         cheese, minced meat, minced fish, yoghurt, sorbet, sherbet,         pudding, a vegetable puree, a fruit puree, a dough, ice milk,         custard, water-ices, slush ice, smoothies, ice cream, granitas,         paste and meat.

    -   376. A method for increasing the cold resistance of a cosmetic         product capable of being applied to the skin of an individual,         said method comprising the step of contacting the polypeptide         according to any of items 1 to 209 to a cosmetic product,         thereby increasing the cold resistance of the cosmetic product         while being applied on the skin.

    -   377. A method for increasing the moisture content of a product         capable of absorbing water, said method comprising the step of         contacting the polypeptide according to any of items 1 to 209,         thereby increasing the moisture content of the product.

    -   378. A method for liminating a tumour by surgery, said method         comprising the step of injecting the polypeptide according to         any of items 1 to 209 into the tumour prior to subjecting said         tumour to a freezing step, thereby enhancing the killing of the         tumour.

    -   379. A method for controlled removal of adipose tissue by         surgery, said method comprising the step of injecting the         polypeptide according to any of items 1 to 209 into the adipose         tissue prior to removing the adipose tissue.

    -   380. A method for inhibiting clathrate formation in a crude oil         product, said method comprising the step of adding the         polypeptide according to any of items 1 to 209 to the crude oil         product, thereby inhibiting clathrate formation.

    -   381. A method for stabilising a biological sample during drying         or during subjection to high or low osmolalities, said method         comprising the step of contacting the polypeptide according to         any of items 1 to 209, thereby stabilizing the biological sample         during drying or during subjection to high or low osmolalities.

    -   382. The method of item 381, wherein the biological sample         comprises one or more of a polypeptide, microsomes, micelles,         whole blood, blood plasma, blood platelets, red blood cells,         semen, gametes.

    -   383. The method of item 381, wherein the biological sample         comprises a cell culture.

    -   384. The method of item 383, wherein the cell culture comprises         one or more of insect cells, mammalian cells, rodent cells and         human cells.

    -   385. A method for purifying one or more molecules from a         composition comprising different molecules, said method         comprising the step of performing said purification in the         presence of a composition comprising the polypeptide according         to any of items 1 to 209, thereby allowing the purification to         take place at temperatures below the freezing point of the         composition comprising the different molecules.

    -   386. The method of item 385, wherein the molecules of the         composition comprising different molecules are selected from the         group consisting of polypeptides, peptides, amino acids, sugars,         fatty acids, DNA molecules, RNA molecules, phospholipids,         organels, such as e.g. mithocondria, ribosomes etc., adenosine         triphosphate.

    -   387. A method for improved dehydration of a composition to be         dehydrated, said method comprising the step of contacting said         composition with the polypeptide according to any of items 1 to         209, and dehydrating said composition.

    -   388. A composition comprising the polypeptide according to items         1 to 209.

    -   389. A composition comprising the polypeptide according to items         1 to 209, wherein the polypeptide is distributed superficially,         either homogenously or heterogenously.

    -   390. A composition comprising the polypeptide the polypeptide         according to items 1 to 209, wherein the polypeptide is         distributed throughout said composition, either homogenously or         heterogenously.

    -   391. A composition intended for freezing comprising the         polypeptide according to items 1 to 209, wherein the polypeptide         is added prior to freezing.

    -   392. A composition intended for freezing comprising the         polypeptide according to items 1 to 209, wherein the polypeptide         is added after freezing.

    -   393. A pharmaceutical composition comprising the polypeptide         according to items 1 to 209.

    -   394. A surface comprising the polypeptide according to items 1         to 209, wherein said surface is ice-repelling.

    -   395. A surface comprising the polypeptide according to items 1         to 209, wherein said surface is ice-binding.

    -   396. A biological sample comprising the polypeptide according to         items 1 to 209.

    -   397. An edible composition comprising the polypeptide according         to items 1 to 209

    -   398. A liquid composition comprising the polypeptide according         to items 1 to 209.

    -   399. A solid composition comprising the polypeptide according to         items 1 to 209.

    -   400. A solid composition comprising the polypeptide according to         items 1 to 209, wherein the polypeptide is distributed         superficially.

    -   401. A solid composition comprising the polypeptide according to         items 1 to 209, wherein the polypeptide is distributed         throughout the sample, either homogenously or heterogenously.

The invention will now be described in more detail by describing the recombinant expression, purification and characterization of antifreeze proteins from Rhagium mordax

Examples

Two different strategies were used for the identification and purification of anti-freeze polypeptides from R. mordax.

1) The method published in Kristiansen et al. (2005) was used for purifying the anti-freeze polypeptides.

2) Two degenerated primers were designed to match regions in the N- and C-terminal region of the R. Inquisitor AFP (FIG. 1). These were used to amplify cDNA regions from R. mordax RNA isolated from winter-collected animals in a conventional RT-PCR reaction. Such cDNA encodes the central region of putative AFP's from R. mordax. By this approach, the sequence of central portions (99-108 aa) of two types (families) of polypeptides were obtained.

3) Full length AFP sequences were subsequently obtained for 9 isozymes. Primers specific for each of the central portions of the cDNA encoding putative AFP's were employed in combination with amplification of the pertinent 5′ ends (5′RACE) using the Clontech Smart RACE cDNA amplification kit. Once the 5′ ends of each cDNA is obtained, this sequence will be used for obtaining full length clones. This is done using a primer matching the extreme 5-end of each cDNA in combination with a primer matching the mRNA poly-A tail in a RT-PCR reaction of R. mordax RNA.

In total, 9 full length cDNAs encoding various isoforms of the group I AFP's were obtained and the encoded polypeptides deduced.

Expression and Purification

Six isoforms of antifreeze proteins (AFPs) from Rhagium mordax RmAFP has been cloned. FIG. 5 shows an SDS-PAGE gel of wild type (wt) soform RmAFP1 and deletion variants (Δ 2-9, WC (Trp-Cys)). The RmAFP variants were constructed as deletions of the ice binding domains one at a time from the C-terminal of RmAFP1 (Δ2-Δ9) (for example Δ4 indicates that putative ice binding domain 4-9 has been deleted) and a variant containing a Trp and Cys residue in the C-terminal (WC). These were cloned into pGEX vector system (GE Healthcare) and transformed in E. coli (strains: Origami, BL21). The proteins were expressed as a fusion protein to Glutathione-S-transferase (GST) containing a thrombin cleavage site between GST and RmAFP. The cells were lysed by French press and the purification was based on affinity chromatography using reduced glutathione covalently attached to sepharose beads and size exclusion chromatography. The arrows in FIG. 5 indicate the positions of the deletion derivatives of AFP1.

Activity Measurements

Wild type isoforms of Rhagium mordax antifreeze proteins and variants of these were tested for activity using a nano-litre osmometer. The results are shown in the table 4 below. The RmAFP variants were constructed as deletions of the ice binding domains one at a time from the C-terminal of RmAFP1 and a variant containing a Trp and Cys residue in the C-terminal. TH_(app) is the thermal hysteresis estimated directly from the assay without considering concentration of AFP, purified or as fusion protein or ice fractionation in the individual measurement; except for the asterisk marked TH which was estimated by extrapolation of a straight line through the points in a plot of TH versus the ice fraction. n is the number of measurements. Not determined, but in progress (N.D.) The fact that we find detectable activity in Δ5-9 and Δ4-9 demonstrates that truncated versions of AFP1 containing 4 and 3 putative ice-binding motifs, respectively, retains the ability to prevent growth of ice crystals. The specific activity of deletion derivatives has not been determined and their activity relative to wt protein is at present unknown.

TABLE 4 AFP Variant # Purification step TH app (° C.) n 1 Purified; fusion 8.0*; 2-6 4 protein 2 Cell lysate/fusion 1.0 3 protein 3 Cell lysate/fusion 1.4 4 protein 4 Cell lysate/fusion 1.1 3 protein 5 Cell lysate/fusion 1.4 3 protein 8 Purified fusion 2.0 3 protein Δ2-9 Cell lysate/fusion <0.1 2 protein Δ3-9 Cell lysate/fusion N.D. — protein Δ4-9 Cell lysate/fusion 0.79 3 protein Δ5-9 Cell lysate/fusion 0.39 2 protein Δ6-9 Cell lysate/fusion N.D. — protein Δ7-9 Cell lysate/fusion N.D. — protein Δ8-9 Cell lysate/fusion N.D. — protein Δ9 Cell lysate/fusion <0.1 1 protein WC Cell lysate/fusion >1 2 protein

Visual Inspection of RmAFP Activity

FIG. 6 illustrates the progression of the “ice growth-explosion” which occurs at the hysteresis freezing point in 1) a solution of RmAFP1 and 2) in serum of the eel pout Zoarces viviparus (a danish fish with type 3 AFP). In both cases a small ice crystal was allowed to anneal in the solution before the temperature was lowered. When decreasing the temperature in the RmAFP solution the initial ice crystal (at the arrow in 1A) does not grow or change shape before the hysteresis freezing point is reached. At the hysteresis freezing point (1B) the ice crystal bursts and due to the super cooled surroundings the solution freezes. Note that in this case the ice growth pattern at the hysteresis freezing point is cauliflower like (1B, 1C). This is in contrast to the events seen in 2) (the Z. viviparus serum) where, upon cooling, the ice crystal slowly changes shape and become a bipyramid with a hexagonal base plane (2A). At the hysteresis freezing point the ice growth is spicular (2B) and all ice in the solution is growing as spicules (2C). In conclusion the RmAFP is able to inhibit all ice growth or change of ice crystal shape (1). This is significantly different from fish antifreeze protein solutions which in general follow the pattern seen in (2).

Physico-Chemical Characterization of RmAFP1-Mass Spectrometry (MALDI-ToF)

Molecular weight determination of cloned and purified Rhagium mordax AFP1 is shown in FIG. 7. The analysis by mass spectrometry of the purified recombinant rRmAFP1 was performed by MALDI-ToF analysis by Alphalyse (Odense, Denmark). Molecular weight determination of rRmAFP1 gave an average value of 12555 Da. This discrepancy may be due to the use of external references when calibrating the apparatus.

Size-Exclusion Chromatography

Dimerization of rRmAFP1 has also been investigated and the result is shown in FIG. 8. Under native conditions rRmAFP behaves as a dimer, since when it is passed over a size exclusion column (Superdex 75, 10/30, GE Healthcare) it has shorter retention times than other proteins in the same Mw range. Mw estimation from SEC of rRmAFP1 gave 28 KDa when the Superdex 75 was calibrated with bovine serum albumin (68 KDa, BSA), trypsin (25 KDa), and human cystatin C (13 KDa). This is shown in FIG. 8A. Likewise RmAFP behaves as a dimer when run in an SDS PAGE giving an estimated Mw of 28 KDa. Lane 1: Bovine serum albumin (BSA); 2: Trypsin; 3: RNAse A; 4 Cystatin C; 5: Lysozyme; 6 RmAFP1; 7: Glutathione S transferase (GST). This is shown in FIG. 8B.

Circular Dichroism Spectroscopy

Circular dichroism spectroscopy (CD) was performed at University of Copenhagen. The results can be seen in FIG. 9. According to FIG. 9A the far UV CD spectrum indicates that the recombinant protein has a defined structure. The near UV CD spectrum, which is shown in FIG. 9B suggests that rRmAFP has a high content of beta-sheet structure.

Differential Scanning Calorimetry

Temperature stability was investigated by differential scanning calorimetry (DSC; Scal, Moscow, Russia). The results are shown in FIG. 10. The transition temperature, Tm, of RmAFP1 was estimated to 47.7° C. The first scan was performed within the temperature range 6-100° C. and the succeeding 11 scans were performed within the temperature range 6-70° C. The asymmetric shape of the curve suggests a dissociation of the protein dimer followed by the unfolding of the monomers. The overall similarity of the curves resulting from 11 scans indicate that the RmAFP is capable of denaturing and renaturing multiple times upon successive heating/cooling cycles without any loss of material. The lower line gives the result of a similar heating cycle performed on a buffer control. Subsequently a sample that had undergone 12 heating-cooling cycles was analyzed for activity and gave a thermal hysteresis, TH_(app), at 0.94° C. which did not differ from the the activity of the staring material. These studies suggest a moderately, stable protein, however, with the noticeable feature of renaturation into a biologically active molecule after denaturation.

Influence of pH on Activity and Stability

The influence of pH on activity and stability has also been investigated. The effect of pH on stability of RmAFP shown as TH at pH 7.2 when pre-incubated 1 h at the indicated pH values are shown in FIG. 11. 

1. An isolated polypeptide comprising or consisting of a sequence of amino acid residues selected from the group consisting of SEQ ID NO:1; SEQ ID NO:2; SEQ ID NO:3; SEQ ID NO:4; SEQ ID NO:5; SEQ ID NO:6; SEQ ID NO:7 and SEQ ID NO:8, wherein said polypeptide is capable of reducing or inhibiting the formation and/or growth of ice crystals.
 2. The polypeptide according to claim 1, wherein the sequence is SEQ ID NO:1.
 3. The polypeptide according to claim 1, wherein the sequence is SEQ ID NO:2.
 4. The polypeptide according to claim 1, wherein the sequence is SEQ ID NO:3.
 5. The polypeptide according to claim 1, wherein the sequence is SEQ ID NO:4.
 6. The polypeptide according to claim 1, wherein the sequence is SEQ ID NO:5.
 7. The polypeptide according to claim 1, wherein the sequence is SEQ ID NO:6.
 8. The polypeptide according to claim 1, wherein the sequence is SEQ ID NO:7.
 9. The polypeptide according to claim 1, wherein the sequence is SEQ ID NO:8.
 10. An isolated polynucleotide comprising a sequence of nucleotides encoding the polypeptide according to any of claims 1 to
 9. 11. The polynucleotide according to claim 10 further comprising an expression signal capable of directing the expression in a suitable host cell of the sequence of nucleotides encoding the polypeptide according to any of claims 1 to 9
 12. A vector comprising the polynucleotide according to any of claims 10 and
 11. 13. An isolated, recombinant cell comprising the polynucleotide according to any of claims 10 and 11 or the vector according to claim 12 or the polypeptide according to any of claims 1 to
 9. 14. An edible product comprising the polypeptide according to any of claims 1 to
 9. 15. The edible product according to claim 14, wherein the edible product is frozen.
 16. The edible product according to claim 14, wherein the edible product is a frozen confectionary product.
 17. The edible product according to claim 14, wherein the edible product is an ice cream product or bread.
 18. A solid support material comprising the polypeptide according to any of claims 1 to
 9. 19. A method for producing the polypeptide according to any of claims 1 to 9, said method comprising the steps of i) providing the polynucleotide according to any of claims 10 and 11 or the vector according to claim 12, ii) providing a host cell suitable for the production of the polypeptide according to any of claims 1 to 9 by recombinant expression of the polynucleotide provided in step i), iii) producing the polypeptide according to any of claims 1 to 9, and optionally iv) purifying and/or isolating said polypeptide.
 20. A method for in situ production of a polypeptide according to any of claims 1 to 9, said method comprising the steps of i) providing a fermentable starting material ii) providing a microorganism capable of fermenting said fermentable food starting material and capable of producing the polypeptide according to any of claims 1 to 9 under suitable conditions when fermenting said fermentable food starting material, iii) fermenting said food starting material in the presence of said microorganism, thereby producing a fermented, edible product, wherein said fermented, edible product comprises the polypeptide according to any of claims 1 to
 9. 21. A method for reducing or inhibiting ice crystal formation in a frozen, edible product, said method comprising the steps of i) providing a frozen edible product, or one or more ingredients required for the production thereof, and ii) contacting said product and/or said ingredients, prior to, during, or after, the production of the product, as the case may be, with the polypeptide according to any of claims 1 to 9, thereby reducing or inhibiting ice crystal formation in the frozen, edible product.
 22. A method for reducing or inhibiting ice crystal growth in a frozen, edible product, said method comprising the steps of i) providing a frozen edible product, or one or more ingredients required for the production thereof, and ii) contacting said product and/or said ingredients, prior to, during, or after, the production of the product, as the case may be, with the polypeptide according to any of claims 1 to 9, thereby reducing or inhibiting ice crystal growth in the frozen, edible product.
 23. A method for structuring ice crystals in a frozen, edible product, said method comprising the steps of i) providing a frozen edible product, or one or more ingredients required for the production thereof, and ii) contacting said product and/or said ingredients, prior to, during, or after, the production of the product, as the case may be, with the polypeptide according to any of claims 1 to 9, thereby structuring ice crystals in the frozen, edible product.
 24. A method for modulating the texture or organoleptic qualities of a frozen, edible product, said method comprising the steps of i) providing a frozen edible product, or one or more ingredients required for the production thereof, and ii) contacting said product and/or said ingredients, prior to, during, or after, the production of the product, as the case may be, with the polypeptide according to any of claims 1 to 9, thereby modulating the texture or organoleptic qualities of the frozen, edible product.
 25. A method for monitoring ice crystal formation during the manufacture or storage of a frozen, edible product, said method comprising the steps of i) providing a frozen edible product, or one or more ingredients required for the production thereof, and ii) contacting said product and/or said ingredients, prior to, during, or after, the production of the product, as the case may be, with the polypeptide according to any of claims 1 to 9, and iii) monitoring ice crystal formation at different time points during the manufacture or storage of the frozen, edible product.
 26. A method for performing an in vitro fertilisation (IVF) treatment in a female individual, said method comprising the steps of i) removing one or more oocyte(s) from a female individual, optionally together with a biological sample comprising follicular fluid; ii) freezing the one or more oocyte(s), optionally together with the biological sample, in the presence of the polypeptide according to any of claims 1 to 9; iii) fertilising one or more of the removed oocytes in vitro; and iv) implanting one or more of the fertilized oocytes into the female individual.
 27. A method for increasing the likelihood or probability of pregnancy in a female individual, said method comprising the steps of ii) removing one or more oocyte(s) from a female individual, optionally together with a biological sample comprising follicular fluid; iii) freezing the one or more oocyte(s), optionally together with the biological sample, in the presence of the polypeptide according to any of claims 1 to 9; iv) fertilising one or more of the removed oocytes in vitro; and v) implanting one or more fertilized oocytes into the female individual, wherein the freezing of the one or more oocyte(s) in the presence of the polypeptide according to any of claims 1 to 9 increases the likelihood or probability of pregnancy.
 28. The method of any of claims 26 and 27, wherein the sample further comprises granulosa-lutein cells or follicular cells and optionally also other ovarian cells recovered from the ovarian follicles of the female individual.
 29. The method of claim 28, wherein the sample further comprises frozen cells from the environment of an oocyte. 